BGC0002552 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0002552 (Amycolatopsis sp. CP2808)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS,NRPS-like	1	82,440	High	ansacarbamitocin	PKS

BGC0002552 - Region 1 - NRPS-like,T1PKS

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Location: 1 - 82,440 nt. (total: 82,440 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX) and not alpha_am_amid)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 82,441
Label		BGC0002552

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase oxygenase

QGJ79685.1: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

acm11: monooxygenase FAD-binding

smcogs: SMCOG1050: monooxygenase FAD-binding

acm13: 3-hydroxybutyryl-CoA dehydrogenase

smcogs: SMCOG1095: 3-hydroxybutyryl-CoA dehydrogenase

acm15: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

acm44: oxidoreductase

smcogs: SMCOG1079: oxidoreductase

Transferases: aminotransferase glycosyltransferase halogenase

acm12: conventional flavin-dependent halogenase

halogenases: conventional flavin-dependent halogenase
smcogs: SMCOG1119: halogenase

acm25: glycosyltransferase, MGT family

smcogs: SMCOG1062: glycosyltransferase, MGT family

acm43: DegT/DnrJ/EryC1/StrS aminotransferase

smcogs: SMCOG1056: DegT/DnrJ/EryC1/StrS aminotransferase

Hydrolases: hydrolase phosphatase

acm16: FkbH like protein

smcogs: SMCOG1256: FkbH like protein

acm45: HAD-superfamily hydrolase, subfamily IA, variant

smcogs: SMCOG1115: HAD-superfamily hydrolase, subfamily IA, variant

Ligases: synthase

acm47: 3-dehydroquinate synthase

smcogs: SMCOG1183: 3-dehydroquinate synthase

acmA: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

acmB: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

acmC: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

acmD: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002552.2	1.68	PKS	ansacarbamitocin	Amycolatopsis sp. CP2808
BGC0001276.3	1.39	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001273.3	1.14	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	1.11	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0002011.3	1.00	PKS	ansacarbamitocin A	Amycolatopsis alba DSM 44262
BGC0002191.2	0.98	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001284.4	0.98	PKS	alternariol	Parastagonospora nodorum SN15
BGC0000041.3	0.97	PKS	oronofacic acid	Pseudomonas syringae
BGC0002341.2	0.94	other (other)	3,5-dibromo-p-anisic acid	Planctomycetales bacterium 10988
BGC0002192.3	0.92	PKS	FR901512	fungal sp. No.14919

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002552.2	1.00	PKS	ansacarbamitocin	Amycolatopsis sp. CP2808
BGC0002011.3	0.77	PKS	ansacarbamitocin A	Amycolatopsis alba DSM 44262
BGC0001273.3	0.76	PKS	asperlactone	Aspergillus ochraceus
BGC0002341.2	0.76	other (other)	3,5-dibromo-p-anisic acid	Planctomycetales bacterium 10988
BGC0001276.3	0.76	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001244.3	0.76	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001275.3	0.76	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0002191.2	0.66	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001284.4	0.66	PKS	alternariol	Parastagonospora nodorum SN15
BGC0000037.4	0.66	PKS	cichorine	Aspergillus nidulans FGSC A4

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 82440:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (AHBA - Me-redmal - ccmal) + (Me-ccmal - mal) + (Me-ohmal) + (Me-ccmal - ccmal)
Non-colinear ordering:: acmA + acmB + acmC + acmD

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 0 - 40003:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (Me-ohmal) + (Me-ccmal - ccmal)
Non-colinear ordering:: acmC + acmD

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 34587 - 82440:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (AHBA - Me-redmal - ccmal) + (Me-ccmal - mal)
Non-colinear ordering:: acmA + acmB

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

acmD: mmal - mal

PKS_AT (549..840): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 192.9
Ethylmalonyl-CoA: 167.2
Methoxymalonyl-CoA: 159.3
2-Methylbutyryl-CoA: 101.8
trans-1,2-CPDA: 100.1
Malonyl-CoA: 96.6
Propionyl-CoA: 86.0
CHC-CoA: 83.7
Isobutyryl-CoA: 62.8
Benzoyl-CoA: 59.8
Acetyl-CoA: 41.8
fatty_acid: 34.3
3-Methylbutyryl-CoA: 27.7
inactive: 26.8

PKS_AT (2160..2434): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 79.2%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 181.7
inactive: 77.0
Methoxymalonyl-CoA: 65.6
Methylmalonyl-CoA: 58.2
Isobutyryl-CoA: 41.8
Propionyl-CoA: 26.6
Ethylmalonyl-CoA: 25.1
Benzoyl-CoA: 19.5
Acetyl-CoA: 18.4
fatty_acid: 7.9
trans-1,2-CPDA: 6.8
CHC-CoA: 6.6
2-Methylbutyryl-CoA: 6.1
3-Methylbutyryl-CoA: 0.0

acmC: mmal

PKS_AT (556..847): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 195.2
Ethylmalonyl-CoA: 172.8
Methoxymalonyl-CoA: 163.9
Malonyl-CoA: 114.7
trans-1,2-CPDA: 103.2
2-Methylbutyryl-CoA: 99.7
CHC-CoA: 92.8
Propionyl-CoA: 87.0
Isobutyryl-CoA: 72.9
Benzoyl-CoA: 54.9
fatty_acid: 48.6
3-Methylbutyryl-CoA: 47.4
Acetyl-CoA: 31.7
inactive: 26.8

acmA: AHBA - mmal - mal

CAL_domain (34..432): AHBA

minowa: AHBA

Prediction, score:

AHBA: 451.3
shikimic_acid: 279.4
Acetyl-CoA: 11.7
fatty_acid: 0.0
NH2: 0.0

PKS_AT (1421..1712): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 198.2
Ethylmalonyl-CoA: 175.5
Methoxymalonyl-CoA: 165.6
2-Methylbutyryl-CoA: 132.6
Malonyl-CoA: 103.8
Propionyl-CoA: 100.7
CHC-CoA: 94.2
trans-1,2-CPDA: 86.6
Isobutyryl-CoA: 80.5
Benzoyl-CoA: 61.6
Acetyl-CoA: 49.7
3-Methylbutyryl-CoA: 49.0
fatty_acid: 37.5
inactive: 19.6

PKS_AT (3314..3585): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 70.8%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 194.7
inactive: 80.3
Methoxymalonyl-CoA: 66.0
Methylmalonyl-CoA: 48.6
Isobutyryl-CoA: 34.8
Propionyl-CoA: 24.8
Ethylmalonyl-CoA: 24.8
Acetyl-CoA: 18.7
fatty_acid: 16.6
Benzoyl-CoA: 14.7
trans-1,2-CPDA: 10.1
CHC-CoA: 6.8
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

acmB: mmal - mal

PKS_AT (547..840): mmal

ATSignature: Ethylmalonyl-CoA, Methylmalonyl-CoA, Methoxymalonyl-CoA

Top 3 matches:: Ethylmalonyl-CoA: 95.8%; Methylmalonyl-CoA: 95.8%; Methoxymalonyl-CoA: 95.8%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 182.9
Ethylmalonyl-CoA: 171.5
Methoxymalonyl-CoA: 169.1
2-Methylbutyryl-CoA: 101.4
trans-1,2-CPDA: 97.6
Malonyl-CoA: 96.1
CHC-CoA: 86.6
Propionyl-CoA: 79.2
Isobutyryl-CoA: 77.1
Benzoyl-CoA: 54.2
3-Methylbutyryl-CoA: 29.8
Acetyl-CoA: 28.6
fatty_acid: 20.3
inactive: 19.4

PKS_AT (2192..2480): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 79.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 179.4
inactive: 73.9
Methoxymalonyl-CoA: 66.6
Methylmalonyl-CoA: 42.8
Propionyl-CoA: 28.4
Ethylmalonyl-CoA: 23.0
fatty_acid: 18.0
Acetyl-CoA: 15.8
Isobutyryl-CoA: 10.3
2-Methylbutyryl-CoA: 10.0
Benzoyl-CoA: 6.6
CHC-CoA: 6.0
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

Link to PARAS substrate predictor