LOCUS       BGC0002554              7849 bp    DNA     linear   BCT 09-DEC-2020
DEFINITION  Streptomyces exfoliatus 2419-SVT2 DNA, carquinostatin A biosynthetic
            gene cluster.
ACCESSION   BGC0002554
VERSION     BGC0002554
KEYWORDS    .
SOURCE      Streptomyces exfoliatus
  ORGANISM  Streptomyces exfoliatus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 17:59:20
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 7849
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..7849
                     /db_xref="taxon:1905"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces exfoliatus"
                     /strain="2419-SVT2"
     misc_feature    1..7849
                     /note="carquinostatin A biosynthetic gene cluster"
     protocluster    1..7849
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="True"
                     /core_location="[3822:4836]"
                     /cutoff="20000"
                     /detection_rule="(Terpene_synth or Terpene_synth_C or
                     phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7
                     or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      3823..4836
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(Terpene_synth or Terpene_synth_C or
                     phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7
                     or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
     cand_cluster    1..7849
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(Terpene_synth or Terpene_synth_C or
                     phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7
                     or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)"
                     /kind="single"
                     /product="terpene"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..7849
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0002554"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..7849
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="terpene"
                     /region_number="1"
                     /rules="(Terpene_synth or Terpene_synth_C or phytoene_synt
                     or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps
                     or fung_ggpps2 or trichodiene_synth or TRI5)"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            145..1104
                     /gene="cqsB1"
     CDS             145..1104
                     /codon_start=1
                     /gene="cqsB1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) fabH"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1084:3-oxoacyl-(acyl carrier protein) synthase III
                     (Score: 203.4; E-value: 7.6e-62)"
                     /gene_kind="biosynthetic-additional"
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="BBF24923.1"
                     /sec_met_domain="fabH (E-value: 1e-44, bitscore: 142.1,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPERVVSNSELPSEGGDLSDHAFFAGVHERRFAWPDYTSAQLGAK
                     ALEKLLERNGVAAADVDLIICASQLNDAFSPGVGTAIQYAVGATKAAVLQVDNGCCSWI
                     SSINTARAFIDSGQYRTVAVVTVTNFVSRLEEFQKAPESRVLGDGASATLVTAGDEPTI
                     LSIHEQAFGENWGALKVEPDTVEGMEIPYWSAGSGALTVKFDQSMLARLYTVTMEQLPQ
                     AVDIALEKAGVENKDIAFLITHQPNEKYVAEWRKRCGFDDSRAHDTLGVYGNMFQGTLP
                     VTFADALDKKMISPGDLIAFATFSHGGELVGSMVWRWN"
     gene            1182..1862
                     /gene="cqsB2"
     CDS             1182..1862
                     /codon_start=1
                     /gene="cqsB2"
                     /product="hypothetical protein"
                     /protein_id="BBF24924.1"
                     /transl_table=11
                     /translation="MSQRVPDESGLAQNYVLDRSDLQGLDLVWNENTGMDDMMKLMESK
                     TKETYDHGEIFGQYCSLAEHINVPYDIVFEYAANARSLEEWTYSIRNMKHLGGGLYRAD
                     EMIQPNTDIYIRAEAQKGPEHGLVVYPCAWDQGHELWMRYYMTIIDSSKVLDKPGTVVL
                     WTNCKHPYYDRSTENVPDYIAEGRARTDRVWVGDIWPVFHAGHSIEMGNLKRILEHRFG
                     AGKA"
     gene            1935..3713
                     /gene="cqsB3"
     CDS             1935..3713
                     /codon_start=1
                     /gene="cqsB3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TPP_enzyme_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TPP_enzyme_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1055:pyruvate oxidase/decarboxylase (Score: 374.8;
                     E-value: 1.3e-113)"
                     /gene_kind="biosynthetic-additional"
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="BBF24925.1"
                     /sec_met_domain="TPP_enzyme_N (E-value: 9.3e-43, bitscore:
                     134.9, seeds: 75, tool: rule-based-clusters)"
                     /sec_met_domain="TPP_enzyme_C (E-value: 4.3e-30, bitscore:
                     93.7, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRLADALVTVLRDLDTGYVFGVSGANIEHVHDAIERLGQGRLTSV
                     LAKREDGAANMADARARVHRTLGVCCATSGGGMMNLVAGLAESYAESVPVLALVGQPPA
                     ALDGRGSFQDSSGIGRTVDGQALLGAITKKTVRVTGAEGFWDLLRDAIGTCLSGRKGPV
                     ALLLPRDVYELEVGDLPADWPAHLGAFIEPEPLPEQDVAALFDRLREATAPVLLLGHGN
                     RRSCDPEAVRVFAERTGIPVVTTMGARGEFPHDSDLYLGVVGEGGHPSAAEYVAKSDLV
                     VLVGTGLAMMTRRALGDWDPARAGAVNIDLGEIERSGLADTTVRGDIGEVFRLLERLRA
                     ERPFTAPPVAGYELTRFVARPAVPVPGRTGRKDEDALLQSEAVRLLQEHLPQGGHLLYD
                     AGNCAVAAMHYGSVPAGSSSTIALGMGGMGYSVGAAVGAQLGSPEGTRTVVFCGDGAFL
                     MSGLEAHTAVELGLPILYVVFNNGMHGMCATRQQRFFDSRITAVDYTPVDVSTVARGLG
                     TPDRLWVGSAGTAAELTRQLDEYRRHTHLPGVLELRLTVEEEPPFASFMPAGAPTVPVP
                     GQRQAAVSYEMTGSAL"
     gene            3823..4836
                     /gene="cqsB4"
     CDS             3823..4836
                     /codon_start=1
                     /gene="cqsB4"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: phytoene_synt"
                     /gene_kind="biosynthetic"
                     /product="phytoene synthase"
                     /protein_id="BBF24926.1"
                     /sec_met_domain="phytoene_synt (E-value: 3.4e-37, bitscore:
                     117.2, seeds: 8, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTVQELDLAGITDSGLRADYIASSQLFRKIGRGRFLGRYMMHPAK
                     RPYFDTFFSFVCYIDDLADDINLSVDVRARRLDEWQRTYLAIAKGEDLTSEHPLSLSEQ
                     TDAALARALVHTLRTWDLPYLRVPEFVDGHRKALTTYEYADQEELDDFLETVTLLPAIW
                     INQIFEPISEDAEELCRHTITAFQLLDFIWDLREDLDLGRLYLPLDHLARFGLTRADLD
                     RQIGSGYISDALRELIQFEIDIAREHMNAGRSWPQTLHPTARIFMETDIQTHDSMFPEM
                     IKDDYAFFKSPRRGLDFVSGRMIPRTAKAIARARKANQQATRAGYRIRPPYRGTEA"
     gene            4833..5843
                     /gene="cqsB5"
     CDS             4833..5843
                     /codon_start=1
                     /gene="cqsB5"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) fabH"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1084:3-oxoacyl-(acyl carrier protein) synthase III
                     (Score: 375.6; E-value: 3.1e-114)"
                     /gene_kind="biosynthetic-additional"
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="BBF24927.1"
                     /sec_met_domain="fabH (E-value: 4.6e-115, bitscore: 373.2,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTRGAILSGLGTWLPPQVVTNDMLSSELDTTDEWIRTRTGIARRH
                     IASPGMSTGHLATEAARRALKSAGTDRVDVVIVATSTPDRPCPATAPVVASSLGLTGTG
                     AFDVAAVCTGFVYALATAAGLIAGQVADSALVIGADTFSTILDPRDRTTRVIFGDGAGA
                     VVLRAGDQAEEGALRGFDLGSDGTGVDLITVPGGGSEQRLSGAEAAPGDRYFHMDGPQV
                     FTRAVRYMTASVRKVLDAAALAPEDVAHLVPHQANVRILDTCARELGLPAERVVKNIDL
                     VGNTVAASIPLALAAGAADGRLRAGDPVVLTGFGGGLTWGSALLTWPGGLTVIDG"
     gene            5878..6117
                     /gene="cqsB6"
     CDS             5878..6117
                     /NRPS_PKS="Domain: PP-binding (26-68). E-value: 1.8e-09.
                     Score: 29.9. Matches aSDomain:
                     nrpspksdomains_cqsB6_PP-binding.1"
                     /codon_start=1
                     /gene="cqsB6"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_kind="biosynthetic-additional"
                     /product="acyl carrier protein"
                     /protein_id="BBF24928.1"
                     /sec_met_domain="PP-binding (E-value: 2.5e-10, bitscore:
                     29.9, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNTIDQNLKALLDERFPQSTDRLFEGASFKDAGMDSMGVVEFIVA
                     LERQFGTPTLNGNELGAESTLQDARDLLTSVGVG"
     aSDomain        5956..6081
                     /aSDomain="PP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_cqsB6_PP-binding.1"
                     /evalue="1.80E-09"
                     /label="cqsB6_PP-binding.1"
                     /locus_tag="cqsB6"
                     /protein_end="68"
                     /protein_start="26"
                     /score="29.9"
                     /tool="antismash"
                     /translation="ASFKDAGMDSMGVVEFIVALERQFGTPTLNGNELGAESTLQD"
     gene            6117..7094
                     /gene="cqsB7"
     CDS             6117..7094
                     /NRPS_PKS="Domain: Aminotran_1_2 (13-323). E-value:
                     4.4e-40. Score: 129.7. Matches aSDomain:
                     nrpspksdomains_cqsB7_Aminotran_1_2.1"
                     /codon_start=1
                     /gene="cqsB7"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_1_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1019:aminotransferase (Score: 105.5; E-value:
                     4.9e-32)"
                     /gene_kind="biosynthetic-additional"
                     /product="aminotransferase"
                     /protein_id="BBF24929.1"
                     /sec_met_domain="Aminotran_1_2 (E-value: 6.5e-41, bitscore:
                     129.7, seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPRPHLLTLNEHPLPPLDSVAAEIARHAAAVNRYPEFYPERLRGI
                     VADWLGLPGEQVVLGAGSVGVALQALQASVPAGGGLAYGWRNFDAYPLLADMVGARRIE
                     VPLLPGGHQDLEALAKAVQDADAVIVCNPHNPTGQLVPGDELREFIGRVPEHVLVVLDE
                     AYIEFVAPSGRTDSLGWVREFPNLLVLRTFSKAWGLANLRVGYGVTSPRWAARIGRYQL
                     PYALSTLSAVAVETSLRAQPELEERIAVIVGERERLSAGLARGGWTVLPSHTNFLWLDE
                     PERAEDIRLALAGEGVQARVYAGEGVRLTVGDSDANDSVLRALA"
     aSDomain        6156..7085
                     /aSDomain="Aminotran_1_2"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_cqsB7_Aminotran_1_2.1"
                     /evalue="4.40E-40"
                     /label="cqsB7_Aminotran_1_2.1"
                     /locus_tag="cqsB7"
                     /protein_end="323"
                     /protein_start="13"
                     /score="129.7"
                     /tool="antismash"
                     /translation="LPPLDSVAAEIARHAAAVNRYPEFYPERLRGIVADWLGLPGEQVV
                     LGAGSVGVALQALQASVPAGGGLAYGWRNFDAYPLLADMVGARRIEVPLLPGGHQDLEA
                     LAKAVQDADAVIVCNPHNPTGQLVPGDELREFIGRVPEHVLVVLDEAYIEFVAPSGRTD
                     SLGWVREFPNLLVLRTFSKAWGLANLRVGYGVTSPRWAARIGRYQLPYALSTLSAVAVE
                     TSLRAQPELEERIAVIVGERERLSAGLARGGWTVLPSHTNFLWLDEPERAEDIRLALAG
                     EGVQARVYAGEGVRLTVGDSDANDSVLRA"
     gene            complement(7167..7739)
                     /gene="cqsB8"
     CDS             complement(7167..7739)
                     /codon_start=1
                     /gene="cqsB8"
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="BBF24930.1"
                     /transl_table=11
                     /translation="MTTETSGGPAAEPILLELVDEEGRTIGVAEKLSAHQAPGHLHRAF
                     SVFLFDTEGRMLLQQRALTKYHSPGVWSNTCCGHPYPDEPAQEAAARRVGEELGLHGVP
                     LEPAGTVTYEHPDEISGLVEKEYNHLFVGLVKDTPEPDPAEIHDYRFVTSGELAALRAE
                     TSFSAWFPTVLEAALPGVRRVAPGGGW"
ORIGIN
        1 gggaagcggg gccattcaac gatctctctc attgttctct cggggccccg acatatgtcc
       61 gatcggcatt tccgcgggca gcatcagtac aaagacgggg aaaaattcgt gtcacaaact
      121 cgcattgtcg acgtggttaa tttcatgccg gagcgggtgg tcagtaacag cgagctgccg
      181 agcgagggcg gcgacctcag cgaccacgcg ttcttcgcgg gcgtgcacga gcggcgcttc
      241 gcctggcccg actacacgtc agcccagctg ggcgcgaagg cactcgagaa gctcctggag
      301 cgaaacggcg tagcggcggc cgacgtcgac ctcatcatct gcgcctccca gctgaacgac
      361 gccttctcac ccggcgtcgg caccgccatc cagtacgcgg tcggcgccac caaggcggcc
      421 gtgctccagg tggacaacgg ctgctgttcc tggatctcct ccatcaacac ggcccgggcc
      481 ttcatcgact cgggccagta ccgtacggtc gcggtggtca cggtcaccaa cttcgtctcc
      541 cgcctggagg agttccagaa ggcgcccgag tcccgggtgc tcggtgacgg cgcgtccgcc
      601 accctcgtca cggccggcga cgaaccgacc atcctctcca tccacgagca ggccttcggg
      661 gagaactggg gagcgctcaa ggtcgagccg gacaccgtgg agggcatgga gatcccctac
      721 tggtcggccg gttccggcgc gctcaccgtg aagttcgacc agtccatgct ggcgcggctg
      781 tacaccgtca ccatggaaca gcttccccag gcggtcgaca tcgcgctgga gaaggccggt
      841 gtggagaaca aggacatcgc tttcctgatc acccaccagc cgaacgagaa atacgtcgcc
      901 gagtggcgca agcgctgcgg attcgacgat tcccgggcgc acgacacgct cggcgtctac
      961 ggcaacatgt tccagggcac tcttccggtg acattcgccg acgccctcga caagaagatg
     1021 atctcgcccg gcgatctcat cgccttcgcg accttctcgc acggcgggga actcgtcggt
     1081 tccatggtct ggcgctggaa ctgactttcc cgggcacccc cgcctgcccg ctccctttca
     1141 cttcatttcg ttcattcctt taccactgca tgaggagaag tatgtcccag cgcgttcccg
     1201 acgagtcggg tctggcccag aactacgtcc tggaccggtc cgaccttcag ggcctggacc
     1261 tggtgtggaa cgagaacacc ggcatggacg acatgatgaa gctcatggag agcaagacga
     1321 aggagacgta cgaccacggc gagatcttcg gtcagtactg ctccctcgcc gagcacatca
     1381 acgtccccta cgacatcgtc ttcgagtacg cggccaacgc ccgctccctg gaggagtgga
     1441 cgtacagcat ccgcaacatg aagcacctcg gcggcggtct gtaccgcgcg gacgagatga
     1501 tccagccgaa cacggacatc tacatccgcg ccgaggcgca gaagggtccc gagcacggtc
     1561 tcgtggtcta cccgtgcgcc tgggaccagg gccacgagct gtggatgcgc tactacatga
     1621 cgatcatcga ctcgtcgaag gtgctcgaca agccgggcac cgtggtgctg tggaccaact
     1681 gcaagcaccc gtactacgac cgttcgacgg agaacgtccc ggactacatc gccgagggcc
     1741 gggcgcgcac cgaccgcgtc tgggtcggtg acatctggcc cgtcttccac gccggccaca
     1801 gcatcgagat gggcaacctc aagcggatcc tggagcaccg cttcggcgcc ggcaaggcct
     1861 gagcccgccg cggggcggct gcggccgccc cgctttcgca ccacgacgaa gcacacgcca
     1921 cacatggggg atttatgcgg ctggccgacg ccttggtcac ggttctgcgc gatctggaca
     1981 ccggatacgt tttcggcgtc agcggcgcga acatcgagca cgtccacgat gcgatcgaaa
     2041 ggctcggtca gggccgtctg acgtccgtgc tggcgaagcg cgaggacggg gcggccaaca
     2101 tggcggacgc ccgcgcccgg gtgcaccgca cgctgggcgt gtgctgtgcc acctccggcg
     2161 gcgggatgat gaacctggtc gccgggctgg ccgagtccta cgccgagtcg gtgccggtcc
     2221 tcgcgctggt cgggcagccg ccggccgccc tggacggccg gggttccttc caggactcct
     2281 ccgggatcgg ccggaccgtg gacggccagg ccctgctcgg agccatcacc aagaagaccg
     2341 tccgggtcac cggggcggag ggcttctggg acctgctgcg cgacgccatc ggcacctgcc
     2401 tgagcgggcg caagggcccg gtggccctgc tgctcccccg ggacgtctac gagctggagg
     2461 tcggtgacct cccggccgac tggccggccc acctcggcgc cttcatcgag cccgagccac
     2521 tgcccgagca ggacgtggcc gccctcttcg accggctgcg cgaggccact gccccggtgc
     2581 tgctgctcgg acacggcaac cgccgctcct gcgaccccga ggcggtgcgc gtcttcgccg
     2641 agcggaccgg gatacccgtg gtgaccacca tgggcgcccg cggcgagttc ccccacgaca
     2701 gcgacctgta cctgggcgtc gtcggcgagg gcggccaccc ctcggccgcc gagtacgtgg
     2761 ccaagtcgga cctggtggtc ctggtgggaa ccggcctggc catgatgacc cggcgggcgc
     2821 tgggcgactg ggacccggca cgggccggcg cggtcaacat cgacctcggt gagatcgagc
     2881 gctcgggcct cgcggacacc acggtccgcg gagacatcgg cgaggtgttc cggctgctgg
     2941 aacggctgcg cgccgagcgc ccgttcacgg ccccgccggt cgccggctac gagctgaccc
     3001 gcttcgtcgc ccggcccgcc gtacccgtcc cgggacgcac cggccggaag gacgaggacg
     3061 cgctgctgca gagcgaggcc gtccggctgc tccaggaaca cctgccgcag ggcggccacc
     3121 tgctctacga cgccgggaac tgcgccgtcg ccgccatgca ctacgggtcc gtcccggccg
     3181 gctcctcctc caccatcgcg ctgggcatgg gcggcatggg ctacagcgtc ggggcggccg
     3241 tcggcgccca gctgggctcg ccggagggca cccggaccgt ggtgttctgc ggtgacgggg
     3301 cgttcctgat gagcggcctg gaggcgcaca ccgccgtgga gctgggcctg cccatcctgt
     3361 acgtcgtctt caacaacggc atgcacggca tgtgcgccac gcgccagcag cggttcttcg
     3421 actcccggat caccgccgtg gactacacgc ccgtcgacgt gtccacggtc gcgcggggcc
     3481 tcggcacccc ggaccggctg tgggtcggca gtgccgggac cgccgccgag ctgacccggc
     3541 agctggacga gtaccggcgc cacacgcatc tgcccggggt cctcgaactg aggctcaccg
     3601 tggaggagga gccgcccttc gcctccttca tgcccgccgg cgcccccacc gtgccggtgc
     3661 ccgggcagcg acaggccgcc gtctcgtacg agatgaccgg cagcgctctc tgacgcttcg
     3721 ccctccgtac gccttcgtcc acactccccg tcagccaccc cgccgccgca tccgggcgag
     3781 cggggccccg gggacgcgcg gaccccatcg gaaagaactc acatgacagt gcaagagctg
     3841 gacctcgcag gtatcacgga ctccgggctg cgcgccgact acatcgcgag ctcccagctg
     3901 ttccgcaaga tcgggcgggg ccgcttcctc ggccgctaca tgatgcaccc ggccaagcgg
     3961 ccctacttcg acaccttctt ctcgttcgtc tgctacatcg acgacctggc cgacgacatc
     4021 aacctcagcg tcgacgtccg ggcccgccgg ctggacgagt ggcagcgcac ctacctggcc
     4081 atcgccaagg gcgaggacct caccagcgag cacccgttgt cgctgtcgga gcagacggac
     4141 gccgccctcg cccgcgccct ggtccacacc ctgcgcacct gggacctgcc ctacctgcgc
     4201 gtgcccgagt tcgtcgacgg ccaccgcaag gccctgacga cgtacgagta cgccgaccag
     4261 gaggagctgg acgacttcct ggagacggtc acgctgctgc ccgcgatctg gatcaaccag
     4321 atcttcgagc cgatcagcga ggacgccgag gagctgtgcc ggcacaccat cacggccttc
     4381 cagctgctgg acttcatctg ggacctgcgc gaggacctgg acctgggccg gctgtacctc
     4441 ccgctggacc acctggcccg cttcggcctg acccgggcgg acctggaccg ccagatcggc
     4501 agcggctaca tcagcgacgc gctgcgcgag ctgatccagt tcgagatcga catcgcccgc
     4561 gagcacatga acgccggccg gtcctggccg cagacgctgc acccgacggc gcggatcttc
     4621 atggagaccg acatccagac gcacgactcc atgttcccgg agatgatcaa ggacgactac
     4681 gcgttcttca agagcccccg gcgcggcctc gacttcgtct cgggccggat gatcccgcgc
     4741 acggccaagg ccatcgcccg cgcccgcaag gccaaccagc aggccacccg cgccggttac
     4801 cgcatccggc cgccgtaccg gggcaccgag gcatgacccg cggggcgatc ctgagcggcc
     4861 tgggcacctg gctccccccg caggtggtca ccaacgacat gctctcgtcg gaactggaca
     4921 ccaccgacga gtggatccgg acccgcaccg gcatcgcccg gcggcacatc gcgtcgccgg
     4981 gcatgagcac cgggcacctc gccaccgagg cggcgcgccg agcgctgaag tcggccggca
     5041 ccgaccgcgt ggacgtcgtc atcgtcgcca cgagcacccc ggaccgcccc tgccccgcga
     5101 ccgccccggt ggtcgcctcc tcgctcgggc tgaccgggac cggcgccttc gacgtggccg
     5161 cggtgtgcac gggcttcgtg tacgcgctgg ccacggcggc ggggctgatc gccggtcagg
     5221 tggcggacag cgccctggtg atcggggcgg acaccttctc caccatcctc gacccgcggg
     5281 accgcaccac ccgggtgatc ttcggcgacg gcgccggcgc ggtcgtcctg cgcgccggcg
     5341 accaggccga ggaaggcgcc ctgcgcggct tcgacctggg cagcgacggc accggcgtgg
     5401 acctcatcac ggtgcccggc gggggctccg agcagcggct gagcggggcc gaggcggccc
     5461 cgggcgaccg gtacttccac atggacggcc cgcaggtctt cacccgtgcc gtccggtaca
     5521 tgaccgcatc ggtgcgcaag gtcctggacg ccgccgcgct ggcccccgag gacgtcgccc
     5581 acctggtccc gcaccaggcg aacgtcagga tcctcgacac ctgcgcccgg gaactcgggc
     5641 tgcccgccga gcgggtcgtg aagaacatcg acctggtcgg caacaccgtg gccgcctcca
     5701 tcccgctggc gctcgccgcg ggtgcggccg acggacggct gcgggcgggc gatccggtgg
     5761 tcctcaccgg cttcggcggc ggcctgacct ggggctcggc gctgctcacc tggcccggcg
     5821 gcctcacggt gatcgacggc tgacggcacc ccggcaccct ccacagagaa gagagtcatg
     5881 aacaccatcg accagaacct gaaggcgctg ctcgacgagc ggttccccca gtccaccgac
     5941 cgcctcttcg agggcgcgtc gttcaaagac gccggcatgg actcgatggg ggtcgtcgag
     6001 ttcatcgtgg ccctggagcg gcagttcggc accccgacgc tgaacggcaa cgaactcggc
     6061 gccgagtcca cgctccagga cgcgcgcgac ctgctgacct cggtgggcgt cggctgatgc
     6121 cccggccgca tctgctcacc ctcaacgagc acccgctgcc gccgctcgac tccgtcgccg
     6181 ccgagatcgc ccggcacgcc gccgcggtca accggtaccc ggagttctat cccgagcggc
     6241 tgcgcgggat cgtcgccgac tggctggggc tgcccggtga gcaggtcgtg ctcggcgcgg
     6301 ggtccgtggg cgtcgcgctc caggcgctcc aggcgagtgt gcccgccggc ggggggctgg
     6361 catacgggtg gcgcaacttc gacgcctatc ccctgctcgc cgacatggtg ggggcgcgcc
     6421 gtatcgaggt gcccctgctc ccgggcgggc accaggacct ggaggcgctg gcgaaggcgg
     6481 tccaggacgc ggacgcggtc atcgtgtgca acccgcacaa cccgaccggc caactggtcc
     6541 ccggcgacga gctgagggag ttcatcggcc gggtccccga gcacgtcctg gtggtcctgg
     6601 acgaggcgta catcgagttc gtcgccccgt ccggccgtac ggactcgctg ggctgggtcc
     6661 gggagtttcc gaacctgctg gtgctgcgca ccttctccaa ggcctggggg ctggccaacc
     6721 tgcgtgtcgg ctacggcgtg acgtcccccc ggtgggccgc gcggatcggc cggtaccagc
     6781 tgccgtacgc cctgagcacc ctgtcggcgg tggcggtgga gacgtccctg cgggcccagc
     6841 cggagctcga ggagcggatc gccgtcatcg tcggcgagcg cgagcggctg tccgccgggc
     6901 tggcccgcgg cggctggacg gtgctgccca gccacaccaa cttcctgtgg ctggacgagc
     6961 cggagcgggc cgaggacatc cggctggccc tggcgggcga gggcgtccag gcccgcgtct
     7021 acgccggcga gggcgtccgc ctcaccgtcg gcgacagcga cgccaacgac agcgtgctgc
     7081 gcgccctggc ctgacgcgct catccgaaag agccgctgcc cacggtctcc ccgtgggcag
     7141 cggctctttc gggtgtcagg acgtggtcac cagccgccgc ccggcgcgac gcgccgtacg
     7201 cccggcaggg ccgcctccag gacggtgggg aaccaggccg agaagctggt ctcggcccgc
     7261 agcgcggcca gttccccgga ggtcacgaac cggtagtcgt ggatctcggc ggggtccggc
     7321 tccggggtgt ccttcaccag gccgacgaag aggtggttgt actccttctc gaccaggccg
     7381 gagatctcgt ccgggtgctc gtaggtgacc gtgccggccg gctccagcgg gacgccgtgc
     7441 agacccagct cctcgccgac gcggcgggcc gccgcctcct gcgcgggctc gtccgggtac
     7501 gggtgcccgc agcaggtgtt ggaccacacg cccggcgagt ggtacttcgt cagcgcccgc
     7561 tgctggagca gcatgcgtcc ctcggtgtcg aacaggaaca ccgagaaggc gcggtgcaga
     7621 tggccgggcg cctggtgcgc cgagagcttc tcggcgacac cgatcgtccg gccctcctcg
     7681 tcgacgagtt cgagcaggat cggttccgcg gcggggccgc cggaggtttc cgtggtcatc
     7741 ggatcagccc acccggggag tcgtggtgac ggggagccgg gccgggccgc gcagccacag
     7801 gccgggcttc agcgccacct cgtcgtcgtc cggcttgatc gaggcgcag
//
