LOCUS       BGC0002577              4918 bp    DNA     linear   BCT 01-JUN-2009
DEFINITION  Lactococcus lactis strain LMGT 2081 lactococcin G gene locus,
            complete sequence.
ACCESSION   BGC0002577
VERSION     BGC0002577
KEYWORDS    .
SOURCE      Lactococcus lactis
  ORGANISM  Lactococcus lactis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Lactococcus.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 18:02:20
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4918
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..4918
                     /db_xref="taxon:1358"
                     /mol_type="genomic DNA"
                     /organism="Lactococcus lactis"
                     /strain="LMGT 2081"
     misc_feature    1..4918
                     /note="lactococcin G gene locus"
     protocluster    1..4918
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[535:3475]"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      536..3475
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     cand_cluster    1..4918
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..4918
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0002577"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..4918
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            536..700
                     /gene="lagA"
     CDS             536..700
                     /codon_start=1
                     /gene="lagA"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Antimicrobial17"
                     /gene_kind="biosynthetic"
                     /note="bacteriocin precursor peptide"
                     /product="lactococcin G alpha peptide"
                     /protein_id="ACR43769.1"
                     /sec_met_domain="Antimicrobial17 (E-value: 1.2e-21,
                     bitscore: 65.0, seeds: 2, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKELSEKELRECVGGGTWDDIGQGIGRVAYWVGKAMGNMSDVNQA
                     SRINRKKKH"
     gene            746..928
                     /gene="lagB"
     CDS             746..928
                     /codon_start=1
                     /gene="lagB"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: LcnG-beta"
                     /gene_kind="biosynthetic"
                     /note="bacteriocin precursor peptide"
                     /product="lactococcin G beta peptide"
                     /protein_id="ACR43770.1"
                     /sec_met_domain="LcnG-beta (E-value: 5.4e-26, bitscore:
                     78.9, seeds: 2, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKNNNNFFKGMEIIEDQELVSITGGKKWGWLAWVDPAYEFIKGFG
                     KGAIKEGNKDKWKNI"
     gene            1027..1359
                     /gene="lagC"
     CDS             1027..1359
                     /codon_start=1
                     /gene="lagC"
                     /product="immunity protein lactococcin G"
                     /protein_id="ACR43771.1"
                     /transl_table=11
                     /translation="MFNNIVVFINFLSFVFILVGVDIKYNDNRIKIVHVTFFISFILVM
                     LTSLISHNSIAYSLSQILEILCIICILLLFYILKKTNSLSNRANVVFIIFIVTQVIIII
                     NQLFIR"
     gene            1364..3475
                     /gene="lagD"
     CDS             1364..3475
                     /codon_start=1
                     /gene="lagD"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR01193"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288:ABC
                     transporter related protein (Score: 290.6; E-value:
                     4.1e-88)"
                     /gene_kind="biosynthetic"
                     /product="ABC-transporter"
                     /protein_id="ACR43772.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 3.4e-49, bitscore:
                     154.6, seeds: 36, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR01193 (E-value: 1.9e-252, bitscore:
                     828.0, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKIIYQQDEKDCGVACIAMILKHYGTEITIQRLRELSGTDLDGT
                     SAFGIKKTFEKLGFDAPAFKAGDETWQEKDIPLPLIAHIISEQKYQHYVVVYKVKGDEI
                     WIADPAKGKIRKTISEFSKEWTGVLLFPKPKAEYKPSIERVDSLSTFFPILIKQKSLFI
                     TIFGILSSYYFQGLLDNIIPNQARSTLNILSIGLIFVYLFRVLFEYSRSYLLLLIGQRM
                     SMSIMLGYFKHVLSLPLSFFATRKSGEIISRFLDANKIIDALASATLSLILDIGMVILV
                     GTTLAIQSTQLFLLTLAFLPFYILVVYVFIRSYDKANTEEMSAGAEVNSSIIESLKGIE
                     TIKSYNGENHVYDRVDSEFVTLMKKSFKSVTLDNVQQSLKMVIELISSVLILWLGSSYV
                     IDGKISLGQLITYNALLVFFTEPLQNIINLQVKMQKARVANKRLNEIMSISPEQRNTNI
                     NISKNIFNKDIKLDKVSFSYNMKLPVLRDVSLEIYSKSKVALVGVSGSGKSTLAKLLVK
                     FYDPSEGNITYGDINCQDIENHKLRNHVTYVPQESFFFNGTIIDNLTFGLSHQPEFEKI
                     FRACKAACLVDFINQQPLRFDSVLEEGGNNLSGGQKQRLAIARAILNDSEIIIFDEATS
                     GLDTLLEKEILEYLIKLQDKTIIFIAHHLSIAKACDEIIVLDQGILVGRGTHEELSEKE
                     GVYRRLLNA"
     gene            3498..4868
                     /gene="lagE"
     CDS             3498..4868
                     /codon_start=1
                     /gene="lagE"
                     /product="putative bacteriocin secretion protein"
                     /protein_id="ACR43773.1"
                     /transl_table=11
                     /translation="MNQNNWQNTSQFYSQSHKHFYRWIIYPTVVLFLFILLFLFFGKKE
                     IIIKSQAQITSDLVSKIQIPIESKIIENKLSENLSVKKGEALVKLDIESLKQQKSSLEK
                     EIPLVQNQILANQTFIDSLKQGKSLFTSDDDFGYSNQLQSLLSENTASDYAFKQSIIDH
                     ESEKSTYQNSKNSIQKSISKKQVEKNDWEKIKSAWSSQSNLSGASEDTTSQYLNFQEQL
                     KASSKEEKEQVKVTISSSINDKISQIDQELEQLEMEQSKLTPPASYDNEKSSQEYKKKQ
                     LVEQTIATAKQKRIEFKEAQEKYNLELQEVNKQIQDEIITSPIDGFVHINYNVKDQKII
                     PKGEVIAEIYPEIKPGKIEFTSQIEASDLTQVKSGMRVHFKLDSKGNSPIIMEGKIKEI
                     SANAETSERGSFYVVKGFLKQTNKTPFNSRYGLNGRLSLIVGKKSYFNVLKEMIIKN"
ORIGIN
        1 tactcaaaaa ttatggggaa tttgactatg actacgttag taaactaatt gtagaagaaa
       61 aaataacaga aaataccgtc aagcgtcgag caagtacggt ctgttcatgg ataaattgga
      121 tattatcggt tgtggagtag atattttaat attgaaaagt atattgtgtt gactattcga
      181 acaataataa gatttataaa taaatttatt aaataaaaaa tcactggcaa taatatctag
      241 tgatttttta tttagtgaat tttgatatat aaatatgatt taatactttc gtagaggtta
      301 aagaatagta actatgttgc ctatacaaaa attcaaggag atgtctccac aaatatggaa
      361 aggcgcaaaa aatatctctt tatcaagcat ttattttaat tatttccatc gcaatagtaa
      421 gattggcact taataaaaaa taatactcga tttttagaat aaaattaaat atgatattga
      481 tttatgttac taaatatgat ataatattct aataataaat aaaaggagaa tttttatgaa
      541 agaattatca gaaaaagaat tacgagaatg cgttggcggt ggaacttggg atgatattgg
      601 tcaaggaata ggaagagtcg cttattgggt tggaaaagcc atgggaaata tgagcgatgt
      661 taatcaagct tctagaatta atagaaaaaa gaaacactaa tttataagac ttataatatt
      721 tagcaaaata tggagagtga agtatatgaa aaataataat aattttttca agggtatgga
      781 aataattgaa gatcaagaat tagtttcaat aactggaggg aaaaaatggg gctggctagc
      841 ttgggtagac ccagcttatg aatttatcaa gggctttggt aaaggtgcaa ttaaagaagg
      901 aaataaagat aagtggaaaa atatctgacg agtaactttt gttagattaa ataaggcaag
      961 tagtttagtt aagctatttg cttttattat ggttgaagaa tttacaaaaa gaagggataa
     1021 caatttttgt ttaataatat agtagttttt ataaattttt taagctttgt atttattctt
     1081 gtaggtgtag atattaaata taatgacaat cggataaaga tagtacatgt tacttttttt
     1141 attagtttca ttttagtaat gctaacaagt ttaatatcac ataatagcat tgcatatagc
     1201 ttatcacaaa ttttggaaat tttatgtata atttgtattt tgcttttgtt ttatattttg
     1261 aaaaaaacta actcactaag taatagagct aatgtggtat ttattatttt tatcgttact
     1321 caagttatta taatcataaa tcaattattt attaggtgat tttatgaaaa aaataatata
     1381 tcaacaggat gaaaaagatt gtggagtagc ttgtatagcc atgattttaa aacattatgg
     1441 taccgaaatt actattcaaa ggttgcgtga actttctggg acagatttag atggcacgtc
     1501 tgcttttgga ataaaaaaaa catttgaaaa attaggattt gatgcaccag cattcaaagc
     1561 tggtgatgaa acatggcaag aaaaagatat acccttgcct ttgatagctc acataataag
     1621 tgaacaaaag tatcaacact acgtagtggt ttataaagtt aaaggtgatg agatttggat
     1681 tgctgaccca gcaaagggaa agattagaaa aactatttct gaattttcta aagagtggac
     1741 aggtgtctta ctttttccta aaccaaaagc agaatacaaa ccgtctattg aaagagtaga
     1801 tagtttatca acgttctttc ctatactaat aaaacagaag tcactcttca tcacgatttt
     1861 tggaatctta agttcttact attttcaagg cttattggat aatattattc caaatcaggc
     1921 tcggtcgact ttaaatattc tttccatagg acttattttt gtttatcttt ttcgtgtgct
     1981 ttttgaatat agtcgtagct atcttctact tttaattggg caaagaatga gtatgagcat
     2041 aatgcttggt tattttaaac acgttttgtc actacctctg agtttctttg caaccagaaa
     2101 gtcaggagaa attatttctc gcttcttgga tgctaataaa attattgatg ctcttgctag
     2161 tgccacctta tctttaattc tggatattgg aatggtaatt ctggttggca cgacacttgc
     2221 gattcaaagt actcagcttt ttttgctcac ccttgccttt ctaccttttt atattttggt
     2281 agtatatgtg tttattagga gttacgataa agcaaataca gaagaaatga gtgcaggagc
     2341 tgaagttaat tctagtatta ttgaaagtct aaaaggaatt gaaactatta aatcttacaa
     2401 tggagaaaat catgtctatg atcgtgtaga ttcagaattt gtaactttaa tgaaaaagtc
     2461 ttttaaatcg gtcacacttg ataatgtaca acagagttta aaaatggtta ttgaacttat
     2521 aagtagtgta ttgatactat ggctagggtc aagttatgtt atagatggaa aaataagtct
     2581 aggacaatta attacctata atgctttact tgtatttttt actgaacctt tacaaaatat
     2641 tattaatttg caagtgaaaa tgcaaaaagc acgtgtagca aataaacgtt tgaacgaaat
     2701 catgtcaata tctccagaac aaagaaatac gaatattaat atatcgaaaa atatatttaa
     2761 taaggatata aaattagata aagtaagttt ttcttataat atgaagcttc ccgttttaag
     2821 agatgtttct ttagaaatat attccaaaag taaggttgct cttgttggtg tgagcggttc
     2881 aggcaagtct acactagcta aactattagt aaaattctat gatccctctg agggaaatat
     2941 cacttatggt gatataaatt gtcaagatat tgaaaatcat aaattaagaa atcatgttac
     3001 ttatgttcct caggaatctt tcttttttaa tggtacaatt atagataatt taacttttgg
     3061 tcttagtcat caaccagagt ttgaaaaaat ttttagagca tgtaaagctg cttgtcttgt
     3121 tgattttatt aaccaacaac ctttaagatt tgattcagtt cttgaagaag gaggaaataa
     3181 tctatcagga ggacaaaagc aacgcttagc aatagctaga gctattttaa atgattctga
     3241 aataattatt tttgatgaag caactagtgg tcttgatacc ttattagaaa aagagatttt
     3301 agaatattta attaagttac aggataaaac tatcattttt attgcccacc atctatcaat
     3361 agctaaagcc tgtgatgaaa tcattgttct agatcaagga atattggttg ggagaggaac
     3421 acacgaagaa ttatctgaaa aagagggtgt atataggaga ttattaaacg cataacaact
     3481 tacggagtaa tacaaacatg aatcaaaata attggcaaaa tacttcacag ttctatagtc
     3541 aaagtcacaa acatttctat cgctggatta tttatcctac agtagtttta tttcttttta
     3601 ttcttctttt tttattcttc gggaagaaag aaattattat taaatcccaa gcacaaatta
     3661 catcagattt agtttctaaa atacagattc caattgaatc taaaataata gaaaataaac
     3721 tttctgaaaa tttgtctgtg aaaaaagggg aagcattagt taagcttgat atagagtctc
     3781 ttaaacaaca aaaaagcagt ttagaaaagg aaattccttt agttcaaaac caaattttag
     3841 ctaatcaaac tttcattgat agtctgaagc aaggaaaaag tttgttcact tcagatgatg
     3901 attttggtta tagtaatcaa ttgcaaagct tactatcgga gaatactgct tctgattatg
     3961 catttaaaca aagcataatt gatcatgaga gtgaaaaaag tacttatcaa aattctaaaa
     4021 attcaattca gaaaagcatc tctaaaaaac aagtagaaaa aaatgattgg gaaaaaataa
     4081 aaagtgcttg gagttctcag tcaaatcttt cgggggcttc agaagatact acttcacaat
     4141 atttgaattt tcaagaacaa ctaaaggcta gttcaaaaga agaaaaagaa caagtaaagg
     4201 taactatttc ttctagtatt aatgataaaa tttcacaaat tgaccaggaa ttggaacagt
     4261 tagaaatgga gcagtcaaaa ctaacacctc cagcttctta cgataatgag aaaagtagtc
     4321 aggaatataa aaagaaacag cttgttgaac aaactattgc aacggcaaag caaaaaagaa
     4381 tagaattcaa agaagcgcaa gaaaaatata atttagaact tcaagaagtc aataagcaaa
     4441 tacaagatga aataattacc tcacctattg atggttttgt tcatattaat tataatgtca
     4501 aagatcagaa gattatccct aagggagaag ttattgccga aatttatcca gaaattaaac
     4561 cagggaaaat tgaatttact tcccaaatcg aagcatccga cttaacacaa gttaaatcag
     4621 ggatgcgggt tcattttaaa cttgattcaa agggaaatag ccctatcata atggagggaa
     4681 aaatcaaaga aatctctgcg aatgctgaaa cttcggagcg aggaagcttt tatgttgtaa
     4741 aagggttctt aaagcagaca aataaaactc cttttaatag tcgatatggc ttaaacggtc
     4801 gattatcact tattgttggt aaaaaatcgt attttaatgt tttaaaagaa atgattataa
     4861 aaaactaaaa atatctagtt tttaattagc agtttatcac atgtaacaat ttacaaaa
//
