LOCUS       BGC0002580              1782 bp    DNA     linear   BCT 25-FEB-2020
DEFINITION  Lactococcus cremoris strain 9B4 lactococcin MN gene cluster, partial
            sequence.
ACCESSION   BGC0002580
VERSION     BGC0002580
KEYWORDS    .
SOURCE      Lactococcus cremoris
  ORGANISM  Lactococcus cremoris
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Lactococcus.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   van Belkum,M.J., Hayema,B.J., Jeeninga,R.E., Kok,J. and Venema,G.
  TITLE     Organization and nucleotide sequences of two lactococcal bacteriocin
            operons
  JOURNAL   Appl. Environ. Microbiol. 57 (2), 492-498 (1991)
   PUBMED   1901707
REFERENCE   2  (bases 1 to 1782)
  AUTHORS   Britton,A.P., van der Ende,S.R., van Belkum,M.J. and
            Martin-Visscher,L.A.
  TITLE     The membrane topology of immunity proteins for the two-peptide
            bacteriocins carnobacteriocin XY, lactococcin G, and lactococcin MN
            shows structural diversity
  JOURNAL   Microbiologyopen 9 (1), e00957 (2020)
   PUBMED   31667956
REFERENCE   3  (bases 1 to 1782)
  AUTHORS   van Belkum,M.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUL-2019) Chemistry, University of Alberta, 11227
            Saskatchewan Drive, Edmonton, AB T6G 2G2, Canada
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            ##antiSMASH-Data-START##
            Version               :: False
            Run date              :: 2025-08-15 22:11:19
            NOTE :: This is a single region extracted from a larger record!
            Orig. start           :: 0
            Orig. end             :: 1782
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     gene            <1..285
                     /gene="lcnD"
     CDS             <1..285
                     /codon_start=1
                     /gene="lcnD"
                     /note="bacteriocin secretion protein"
                     /product="LcnD"
                     /protein_id="QIC51114.1"
                     /transl_table=11
                     /translation="STQISGMKVGQKVRFTVQQNLPQPEILTGIINQIDSAPTAFKEGN
                     AYKVSATTTINAKDLPNIRYGLQGKTVTIIGKKTYFNYFLDKIMGRGNQ"
     source          1..1782
                     /db_xref="taxon:1359"
                     /isolation_source="starter culture"
                     /mol_type="genomic DNA"
                     /organism="Lactococcus cremoris"
                     /strain="9B4"
     misc_feature    <1..>1782
                     /note="lactococcin MN gene cluster"
     region          1..1782
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /subregion_numbers="1"
                     /tool="antismash"
     subregion       1..1782
                     /aStool="mibig"
                     /contig_edge="True"
                     /label="ribosomal"
                     /subregion_number="1"
                     /tool="antismash"
     cand_cluster    1..1782
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..1782
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[358:568](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      359..568
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            359..568
                     /gene="lcnM"
     CDS             359..568
                     /codon_start=1
                     /gene="lcnM"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IIc"
                     /gene_kind="biosynthetic"
                     /note="bacteriocin"
                     /product="lactococcin M"
                     /protein_id="QIC51115.1"
                     /sec_met_domain="Bacteriocin_IIc (E-value: 2.2e-13,
                     bitscore: 38.6, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKNQLNFEILSDEELQGINGGIRGTGKGLAAAMVSGAAMGGAIGA
                     FGGPVGAIMGAWGGAVGGAMKYSI"
     gene            594..827
                     /gene="lcnN"
     CDS             594..827
                     /codon_start=1
                     /gene="lcnN"
                     /note="bacteriocin"
                     /product="lactococcin N"
                     /protein_id="QIC51116.1"
                     /transl_table=11
                     /translation="MKKDEANTFKEYSSSFAIVTDEELENINGSGSIWGAIAGGAVKGA
                     IAASWTGNPVGIGMSALGGAVLGGVTYARPVH"
     gene            846..1310
                     /gene="lciM"
     CDS             846..1310
                     /codon_start=1
                     /gene="lciM"
                     /note="immunity protein lactococcin MN"
                     /product="LciM"
                     /protein_id="QIC51117.1"
                     /transl_table=11
                     /translation="MKNDNFLINRFLGIPSKAILSEEGSIKIGKTLSIYGVVSFLFNII
                     IFAFISLLLINNNNALYIVLFGFIANMVLPLPFLYYIKRHNYDEITNKFEIKNKIPWGT
                     GIIITIVYYVLSVFFMPNIPNNAFDGKTALISIGFGIFMTVVFYFIKKVK"
ORIGIN
        1 agtactcaaa tttctggaat gaaagtcggt caaaaagtga gatttacagt acagcaaaat
       61 ttacctcaac ctgaaatttt aactggaata atcaaccaaa tagatagtgc tcccacagct
      121 tttaaagagg gaaatgctta taaagtttct gcgacaacca ctatcaatgc aaaagacctc
      181 ccaaatatcc gatatggtct tcaagggaaa acagtaacca ttataggaaa gaaaacttat
      241 ttcaattact ttttagataa aataatggga agaggcaatc agtagagtta ttaacatttg
      301 ttaacgagtt ttatttttat ataatctata atagatttat aaaaataagg agattattat
      361 gaaaaatcaa ttaaatttcg aaatcctatc agatgaggag ttacaaggta tcaatggcgg
      421 tatcagaggt actggtaagg gcttagctgc cgcaatggtt tcgggggccg caatgggtgg
      481 agctatagga gcttttggtg gtccagtagg tgctattatg ggcgcttggg gcggtgctgt
      541 tggtggtgca atgaaatatt ctatataaaa attgaaagga ttcaggtact aaaatgaaaa
      601 aagatgaagc aaatacattt aaagaatatt cctcatcctt cgctattgtt acggatgaag
      661 aactagaaaa tattaatgga tctggatcaa tctggggagc gattgctgga ggagctgtaa
      721 aaggggcaat cgctgcctct tggactggta atcctgtcgg tatagggatg tcagcattag
      781 gaggcgccgt tttgggtgga gtaacttatg ctagaccagt acactaaaga aaggcttaca
      841 aattaatgaa aaatgataat tttttaataa atagattctt aggaatacct tcaaaagcta
      901 ttttaagtga agaaggttct ataaaaatag gaaaaacatt aagtatttat ggagttgttt
      961 catttttatt taatataatt atctttgctt ttatatctct attattaata aataataata
     1021 acgctttata tattgttttg tttggtttta ttgccaatat ggtattacct ctaccttttt
     1081 tatactatat aaaacgacat aattatgatg aaatcacaaa taaatttgaa ataaaaaata
     1141 aaatcccttg gggaacaggc attattataa caattgtata ttacgtttta tccgtttttt
     1201 tcatgccaaa tatacctaac aatgcttttg acgggaaaac tgctctaata tcaattggtt
     1261 ttggtatatt catgacagta gtattttact ttataaagaa agtaaaatag taggaagatt
     1321 ttcctattgt gcactgtaat ttttggtata tatactttac aatcccaaag gttattatcg
     1381 tgcaatttcg tttctatgtt acatgtaccc caccgagttg atatattatt tggaggtttg
     1441 agcggtggaa ctcaaggttg ataagggtct atgaactctt atccatttag aaagtagtta
     1501 tatgatctat gatgaaatac aaaccttatt taataatgat tggttctttt gttgctgtag
     1561 taattttaaa tcaactatat gcaaaattga gttttactct agccactgtg ttttcaatcc
     1621 tacttatgat tatttatttt attcttttta gactattttt aaatgatgat aaagaaaagt
     1681 aatcacttat agaattaatc attacttacc attaaaagcc agtcgagtta tgaatttgac
     1741 tggcttttaa aaatcattct atcaaaattt aaaaatatcg at
//
