LOCUS       BGC0002582              7207 bp    DNA     linear   BCT 01-MAY-2021
DEFINITION  Streptomyces chrestomyceticus strain NA04264 F420H2-dependent
            reductase, non-ribosomal peptide synthetase, and cytochrome P450
            genes, complete cds.
ACCESSION   BGC0002582
VERSION     BGC0002582
KEYWORDS    .
SOURCE      Streptomyces chrestomyceticus
  ORGANISM  Streptomyces chrestomyceticus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 18:02:46
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             1..489
                     /codon_start=1
                     /product="F420H2-dependent reductase"
                     /protein_id="QUF98524.1"
                     /transl_table=11
                     /translation="MLPAMSRPDRERFLAEPRVGVLGVTDSRGGDRGPLIVPVWYSYEP
                     GGEVVIETGRQTVKAQLLRAAGRFSLCAQDEGRPYRYVSVEGPVTAIDDPLDPALREAL
                     AHRYLDAEEARGYLAATSGQLTEDIAFRMRPQHWRSADFAAFAADFSEGGAGEEAAQR"
     source          1..7207
                     /db_xref="taxon:68185"
                     /mol_type="genomic DNA"
                     /note="scolopendra"
                     /organism="Streptomyces chrestomyceticus"
                     /strain="NA04264"
     protocluster    1..7207
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[547:3772]"
                     /cutoff="0"
                     /detection_rule="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX))"
                     /neighbourhood="20000"
                     /product="NRPS-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      548..3772
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="0"
                     /detection_rule="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX))"
                     /neighbourhood="20000"
                     /product="NRPS-like"
                     /protocluster_number="1"
     cand_cluster    1..7207
                     /SMILES="NC([*])C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX))"
                     /kind="single"
                     /product="NRPS-like"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..7207
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0002582"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..7207
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS-like"
                     /region_number="1"
                     /rules="cds((PP-binding or NAD_binding_4) and (AMP-binding
                     or A-OX))"
                     /subregion_numbers="1"
                     /tool="antismash"
     CDS             548..3772
                     /NRPS_PKS="Domain: AMP-binding (36-437). E-value: 1.7e-95.
                     Score: 311.8. Matches aSDomain:
                     nrpspksdomains_QUF98525.1_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (552-620). E-value: 7e-10. Score:
                     30.9. Matches aSDomain: nrpspksdomains_QUF98525.1_PCP.1"
                     /NRPS_PKS="Domain: TD (664-905). E-value: 1.1e-90. Score:
                     295.0. Matches aSDomain: nrpspksdomains_QUF98525.1_TD.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRPS-like: AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRPS-like: PP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRPS-like: NAD_binding_4"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002:AMP-dependent synthetase and ligase (Score:
                     323.3; E-value: 3.7e-98)"
                     /gene_kind="biosynthetic"
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="QUF98525.1"
                     /sec_met_domain="AMP-binding (E-value: 4.8e-101, bitscore:
                     326.6, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 1.9e-07, bitscore:
                     20.1, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="NAD_binding_4 (E-value: 3.7e-67, bitscore:
                     214.5, seeds: 102, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTDTYVSSRPLARRPQAPGARTPAPGYPRDSRIPELFEAQAAALP
                     QAPAARHGDRTLTYGQLDAHADALADRLVAGGVRPGDLIGVCGSRSLEALVALLGILKA
                     GCAYVPLDEELPPARLRAMAEDAGISAAVTLPGSTRRVRGLRVSVEVGSLDRPVPERAA
                     GPVPGRATGSAADCAYVAFTSGTTGRPKPVALSHRGVVRLVLSDPGLTPPAPGDGVLHA
                     YSLSSDASTIEIWSALLTGACLVVADREELLSPTALERLFHAGGVTVAYLTTSVFHLVA
                     RTRPEALAGLRFVSAGGEAMDPRLANAVLAACPRTTVVNFYGPTENAVVSTAHVLTPLP
                     EDAAHVPLGRPFGASTCHVLRADGSPARPGEEGELYVGGDGLALGYLGDPQLTAERFVT
                     LPEVEPGGPLYRTGDRAVRHADGLLEYRGRLDRQVKLRGARIELDEVETRLRAHPEVGE
                     AAVEVDGHSLTAYVTATVPGRPLPLADLRAYCAKWLPPQAVPALIPLDRFPVTSGGKID
                     RSRLKPTAPPPGPGDAAEAARRPDEPEATDGLSGLLTEVWHQVLRVRPTPQDDFFLLGG
                     DSLLASEAVTRTLAVLGLDAALGSTLIRALLAAPTLESFTAAVRGVRGGTGGPAGGQEP
                     AVDFAAETGLGFALPPAEGPAPNPHDPKDVLLTGASGFVGGFLLHRLLHATAARVHCPV
                     RATSPAHARQRVRTALTRYGLHLDEADWQRVECFPGDLTQPRLGLDHERADALAQRLDL
                     IVHNGARVNFLYPYQQLRPANVDGTREVVRIAARRRVPVHFVSTVAVVAGFGTAGVREV
                     DEDLPPAHADGLTMGYAESKWVAEGVLRRAAEQGLPVAVYRPYEVTGDRTHGACNTETA
                     ICSLFKMIADTGLAPDIELPMDFVPVDHLAESLVHIATHRPADGRVYHLTNPRPAMLSD
                     VLDRMRAAGFTLRTLPYDAWVAELVRHVAENPTSATAPFVSLCVDRSRTADMSVKEMYL
                     KGTFPVLGRRNAEEALADSGLHCPPVDAALLDRYLEYFFTSGYLTRPAAGPGPASGTGR
                     IPGSEPVSGAEPVSGTAAAPRTEHSR"
     misc_feature    578..580
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     aSDomain        656..1858
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_QUF98525.1_AMP-binding.1"
                     /evalue="1.70E-95"
                     /label="QUF98525.1_AMP-binding.1"
                     /locus_tag="QUF98525.1"
                     /protein_end="437"
                     /protein_start="36"
                     /score="311.8"
                     /specificity="substrate consensus: X"
                     /tool="antismash"
                     /translation="FEAQAAALPQAPAARHGDRTLTYGQLDAHADALADRLVAGGVRPG
                     DLIGVCGSRSLEALVALLGILKAGCAYVPLDEELPPARLRAMAEDAGISAAVTLPGSTR
                     RVRGLRVSVEVGSLDRPVPERAAGPVPGRATGSAADCAYVAFTSGTTGRPKPVALSHRG
                     VVRLVLSDPGLTPPAPGDGVLHAYSLSSDASTIEIWSALLTGACLVVADREELLSPTAL
                     ERLFHAGGVTVAYLTTSVFHLVARTRPEALAGLRFVSAGGEAMDPRLANAVLAACPRTT
                     VVNFYGPTENAVVSTAHVLTPLPEDAAHVPLGRPFGASTCHVLRADGSPARPGEEGELY
                     VGGDGLALGYLGDPQLTAERFVTLPEVEPGGPLYRTGDRAVRHADGLLEYRGRLDRQVK
                     LR"
     aSModule        656..3262
                     /complete
                     /domains="nrpspksdomains_QUF98525.1_AMP-binding.1"
                     /domains="nrpspksdomains_QUF98525.1_PCP.1"
                     /domains="nrpspksdomains_QUF98525.1_TD.1"
                     /final_module
                     /locus_tags="QUF98525.1"
                     /monomer_pairings="X -> X"
                     /starter_module
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       842..883
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_QUF98525.1_0001"
                     /evalue="3.40E-06"
                     /label="NRPS-A_a2"
                     /locus_tag="QUF98525.1"
                     /protein_end="112"
                     /protein_start="98"
                     /score="20.0"
                     /tool="antismash"
                     /translation="LGILKAGCAYVPLD"
     CDS_motif       1079..1138
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_QUF98525.1_0002"
                     /evalue="9.40E-08"
                     /label="NRPS-A_a3"
                     /locus_tag="QUF98525.1"
                     /protein_end="197"
                     /protein_start="177"
                     /score="24.4"
                     /tool="antismash"
                     /translation="AYVAFTSGTTGRPKPVALSH"
     CDS_motif       1253..1285
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_QUF98525.1_0003"
                     /evalue="9.90E+01"
                     /label="NRPS-A_a2"
                     /locus_tag="QUF98525.1"
                     /protein_end="246"
                     /protein_start="235"
                     /score="-2.9"
                     /tool="antismash"
                     /translation="SALLTGACLVV"
     CDS_motif       1652..1738
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_QUF98525.1_0004"
                     /evalue="8.30E-15"
                     /label="NRPS-A_a6"
                     /locus_tag="QUF98525.1"
                     /protein_end="397"
                     /protein_start="368"
                     /score="46.9"
                     /tool="antismash"
                     /translation="PGEEGELYVGGDGLALGYLGDPQLTAERF"
     CDS_motif       1823..1888
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_QUF98525.1_0005"
                     /evalue="6.60E-09"
                     /label="NRPS-A_a8"
                     /locus_tag="QUF98525.1"
                     /protein_end="447"
                     /protein_start="425"
                     /score="28.0"
                     /tool="antismash"
                     /translation="YRGRLDRQVKLRGARIELDEVE"
     aSDomain        2204..2407
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_QUF98525.1_PCP.1"
                     /evalue="7.00E-10"
                     /label="QUF98525.1_PCP.1"
                     /locus_tag="QUF98525.1"
                     /protein_end="620"
                     /protein_start="552"
                     /score="30.9"
                     /tool="antismash"
                     /translation="LTEVWHQVLRVRPTPQDDFFLLGGDSLLASEAVTRTLAVLGLDAA
                     LGSTLIRALLAAPTLESFTAAVR"
     aSDomain        2540..3262
                     /aSDomain="TD"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_QUF98525.1_TD.1"
                     /evalue="1.10E-90"
                     /label="QUF98525.1_TD.1"
                     /locus_tag="QUF98525.1"
                     /protein_end="905"
                     /protein_start="664"
                     /score="295.0"
                     /tool="antismash"
                     /translation="VLLTGASGFVGGFLLHRLLHATAARVHCPVRATSPAHARQRVRTA
                     LTRYGLHLDEADWQRVECFPGDLTQPRLGLDHERADALAQRLDLIVHNGARVNFLYPYQ
                     QLRPANVDGTREVVRIAARRRVPVHFVSTVAVVAGFGTAGVREVDEDLPPAHADGLTMG
                     YAESKWVAEGVLRRAAEQGLPVAVYRPYEVTGDRTHGACNTETAICSLFKMIADTGLAP
                     DIELPMDFVPVDHLAESLV"
     CDS             3769..5022
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007:cytochrome P450 (Score: 377.7; E-value: 1e-114)"
                     /gene_kind="biosynthetic-additional"
                     /product="cytochrome P450"
                     /protein_id="QUF98526.1"
                     /sec_met_domain="p450 (E-value: 6.8e-21, bitscore: 62.8,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSRTEAAAPDAPVPDAAPETLIPVPETAPGTAGPPCAYARLRTEA
                     PVVKAQLPNGETGWLISRYEDARAAFADPRLVRPLLSAWPPREGADAPPPCLPTFLEMT
                     GAHHERVRRTVLPMFGRRQLSFMEPRIRAMAGELVDTMVAGADGGTADLVASYAEPLPL
                     RVLCETVGLPYEDREIYLPHTLALLGAAGLTMEEVLAALYALQDYAADLIARKERDGDG
                     EDYIRLLLALARRPDSEITRDDVVSFVVTMLMAGYKTNIQHTGNALLALLTHPEQLRTL
                     RAAPERIPTAVEELLRYVPLMNAINILVATEDFTLHGQHIRAGDAVVPVPASANRDPAA
                     FAEPDRLDLARTPNPHIAFGHGPHACTGGNLTRMQLTIAIQVLLERLPDLELAVPAESI
                     DWDESTPLRAPARLPVRW"
     CDS             5103..6332
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007:cytochrome P450 (Score: 299.7; E-value: 5.2e-91)"
                     /gene_kind="biosynthetic-additional"
                     /product="cytochrome P450"
                     /protein_id="QUF98527.1"
                     /sec_met_domain="p450 (E-value: 1.4e-22, bitscore: 68.3,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTPPQAPASGQAAHEEPLPRYPFSTRGDRLAPELDELRGRCPVA
                     RVNTNSGEQAWLVTGYELARQVLRDRSFARSVLGEADRPTQDAPILAPELLDAMNHLQQ
                     AGLRDEVLKALGRDQPDLPADWVAEITAEGLDTMVRDGSPGDLQRHFADWVAAQCMCRL
                     LGLPFADRDWLAVRADLDLTMVTPTPEELARNWEEIRAYMDAHMSDRRPGEPRGLIDRL
                     ADLNATHEGLTRRQLSNIVAVLFVSGYEDFASFLGVAAYNLLQRPEVMSAVRAEPETMP
                     QCVEELLRCSVVLGNAIPRFVTKDAEIGPAQVKEGEMILLSLDAVNYDSAAFPDPKSFT
                     PARTPNPHLRFGYGRHHCPGAHLVRRQSEVAFRVLLDRLPGIRLAVPPAEVPWHPNRMA
                     IMPAEVPVTW"
     CDS             6458..7207
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abhydrolase_6"
                     /gene_kind="biosynthetic-additional"
                     /product="cytochrome P450"
                     /protein_id="QUF98528.1"
                     /sec_met_domain="Abhydrolase_6 (E-value: 8.6e-10, bitscore:
                     28.0, seeds: 455, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPTKTLQIPTPDGQADAFAAFPDDGERHPGVLLYPDAFGVRPELE
                     RKARELAGHGYYVLVPNLYYRRGPAPVIDLPEHIGEEARPAVFAQVMPLMEEHTTERVL
                     RDAEAYLTFLTSQPEVGPGPVAVLGYCLGAVLAMRTATAHPGRVAAVAGFHPAPLVTDA
                     PDSPHRLVSELTAEVHIGLAEGDMTPEALSEFTRALDTAGVAHTTETYPDTVHGFTMSD
                     TAAYSPAGLQRHWDRLLPLLERTLTKG"
ORIGIN
        1 atgctgcccg ccatgtcgcg ccccgaccgt gaacgtttcc tcgccgaacc gcgcgtgggg
       61 gtcctgggtg tgacggactc cagaggcggt gaccgggggc ccctgatcgt gccggtctgg
      121 tacagctacg agcccggcgg cgaggtcgtc atagagaccg gccgccagac cgtcaaggcc
      181 cagctcctgc gcgccgccgg gcggttcagt ctctgcgcgc aggacgaggg acggccgtac
      241 cgctacgtca gcgtcgaggg acccgtcacc gcgatcgacg acccgctgga cccggccctg
      301 cgcgaggccc tggcccaccg ctacctggac gccgaggagg cccgcggcta cctggcggcg
      361 acctccggcc agctcacgga ggacatcgcc ttccggatgc gcccgcagca ctggcgcagc
      421 gccgacttcg cggccttcgc ggccgacttc tcggaagggg gagccgggga ggaagccgcc
      481 cagcgctgag cggacccgcc cgcgcaccgc cgtaccgcac gcaagctgtt ggagcgagag
      541 agaccacatg accgacacgt acgtttcttc acgcccgtta gccaggcggc cccaggcccc
      601 gggcgcccgc acgccggcac ccggctaccc acgggacagc cgcatcccgg agctgttcga
      661 ggcacaggcc gcggcgctgc cgcaggcccc ggcggcccgg cacggggacc gcaccctgac
      721 ctacggccaa ctcgacgccc acgccgacgc gttggcggac cgtctggtcg ccggcggggt
      781 ccggccaggt gatctgatag gcgtgtgcgg cagccgctcc ctggaagcgc tggtggcgct
      841 gctgggcatc ctcaaggccg gctgcgcgta cgtaccgctc gacgaggaac tgccgccggc
      901 ccggctgcgg gccatggccg aggacgcggg catcagcgcc gcggtcaccc tgccgggcag
      961 cacgcgccgg gtgcgcggtc tacgcgtgtc agtcgaggtc ggctccctcg accggcccgt
     1021 ccccgagcgc gcggccggcc ccgtccctgg ccgggccacc ggctccgccg ccgactgcgc
     1081 ctacgtcgcc ttcacttccg gcacgaccgg ccggcccaag cccgtggcgc tgtcccaccg
     1141 cggcgtggtc cgcctcgtgc tctccgaccc cggcctcacg ccgcccgccc cgggcgacgg
     1201 agtgctgcac gcctacagcc tgtcctccga cgcctcgacc atcgagatct ggagcgcgct
     1261 gctgaccggc gcctgcctgg tcgtcgccga ccgcgaggaa ctgctctcgc ccaccgccct
     1321 ggagcggctg ttccacgcgg gcggcgtcac cgtggcgtac ctgacgacga gcgtcttcca
     1381 cctcgtcgcc cggacccgcc ccgaggcgct ggccggcctg cggttcgtct ccgcgggcgg
     1441 cgaggcgatg gacccgcgcc tggccaacgc cgtcctcgcg gcctgccccc gcaccacggt
     1501 ggtcaacttc tacggcccga ccgagaacgc cgtggtctcc accgcccatg tgctcacccc
     1561 cctccccgag gacgccgcgc acgtccccct gggacgcccc ttcggcgctt ccacctgcca
     1621 cgtcctgcgg gccgacggct cgcccgcgcg gccgggcgag gagggggagc tgtacgtcgg
     1681 cggcgacggg ctggcgctgg gctacctcgg cgacccgcag ttgaccgccg agcggttcgt
     1741 gacgctgccc gaggtcgagc cgggcgggcc gctgtaccgc accggcgacc gggccgtacg
     1801 gcacgccgac gggctgctgg agtaccgcgg acggctcgac cgccaggtca agctgcgcgg
     1861 cgcccgcatc gagctggacg aggtggagac gcgcctacgg gcccaccccg aggtcggcga
     1921 agcggccgtc gaggtcgacg ggcactccct gaccgcctac gtcacggcca ccgtccccgg
     1981 ccgcccgctg ccgctggccg acctgcgcgc gtactgcgcc aagtggctgc ccccgcaggc
     2041 cgtcccggcc ctgatacccc tggaccgctt cccggtcacc agcggcggca agatcgaccg
     2101 cagccgtctg aagccgaccg cgccaccgcc cggccccgga gacgccgcgg aggccgcccg
     2161 gcgcccggac gagccggagg ccaccgacgg cctgtccggc ctcctcacgg aggtgtggca
     2221 ccaggtgctg cgcgtccggc ccacgcccca ggacgacttc ttcctcctcg gcggcgactc
     2281 tctgctcgcc tcggaggccg tcacccgcac cctcgccgta ctcggcctcg acgcggccct
     2341 gggctccacc ctcatcaggg cgctgctggc cgcgcccacc ctcgaaagct tcaccgccgc
     2401 cgtacgcgga gtccgcggcg gcaccggcgg accggccggc ggccaggaac cggccgtcga
     2461 cttcgccgcg gagaccggac tcggcttcgc cctcccgccc gccgaaggcc cggcaccgaa
     2521 cccgcacgac cccaaggacg tcctgctcac gggcgcttcc ggcttcgtcg gcggcttcct
     2581 gctgcaccgt ctgctgcacg cgacggccgc ccgcgtccac tgccccgtac gggcgacgag
     2641 ccccgcccac gcccggcagc gggtccgcac cgccctcacc cgctacggtc tgcacctcga
     2701 cgaggccgac tggcagcgcg tggagtgctt ccccggggac ctgacccagc cgcggctggg
     2761 gctcgaccac gagcgcgccg acgcgctggc ccagcgcctg gacctgatcg tgcacaacgg
     2821 cgcccgggtc aacttcctct acccctacca gcagttgcgc ccggcgaacg tcgacggaac
     2881 ccgtgaggtc gtccggatcg ccgcgcggcg ccgggtgccg gtgcacttcg tgtccaccgt
     2941 cgcggtcgtc gcgggcttcg gcaccgccgg ggtgcgcgag gtggacgagg acctgccgcc
     3001 ggcccacgcc gacgggctga ccatggggta cgcggagagc aagtgggtcg ccgaaggggt
     3061 gctgcggcgg gcggccgagc agggcctgcc ggtggccgtc taccggccgt acgaggtcac
     3121 gggcgaccgg acgcacggcg cgtgcaacac cgagacggcc atctgctcgc tgttcaagat
     3181 gatcgccgac acggggctgg cccccgacat cgagctgccg atggacttcg tgcccgtcga
     3241 ccatctcgcc gagtccctgg tgcacatcgc cacccaccgg ccggccgacg gccgggtcta
     3301 ccacctgacc aacccgcgcc cggcgatgct gtcggacgtc ctcgaccgga tgcgcgcggc
     3361 gggcttcacc ctgcgcaccc tgccgtacga cgcgtgggtc gccgagctcg tccggcacgt
     3421 cgccgagaac ccgacgagcg ccacggcgcc gttcgtgtcc ctgtgcgtgg accgcagccg
     3481 caccgccgac atgtccgtca aggagatgta cctcaagggc accttcccgg tcctggggcg
     3541 gcgcaacgcc gaggaggcgc tggccgacag cgggctgcac tgcccgccgg tcgacgccgc
     3601 cctgctggac cgctacctgg agtacttctt cacctccggc tacctcacgc gcccggcggc
     3661 cggccccggg cccgcatccg ggaccgggcg aataccgggg agtgagccgg tctccggggc
     3721 cgagccggta tccgggaccg cagccgcacc acgcacggag cacagccgat gagccgaacc
     3781 gaagccgccg ccccggacgc ccccgtcccg gacgccgctc ccgaaaccct catccccgtc
     3841 ccggagaccg cccccggcac cgccgggccg ccgtgcgcct acgcccgcct gcggaccgaa
     3901 gcacccgtcg tcaaggccca gctcccgaac ggcgagacgg gctggctcat cagccgctac
     3961 gaggacgcgc gcgccgcgtt cgccgacccg cggctcgtcc ggcccctgct gtcggcctgg
     4021 ccgccccgcg aaggggccga cgccccgccg ccgtgcctgc cgaccttcct ggagatgacg
     4081 ggcgcgcacc acgagcgtgt acggcgcacc gtcctgccga tgttcggccg gcggcaactg
     4141 tccttcatgg agccccgcat ccgcgcgatg gccggggaac tcgtcgacac gatggtggcc
     4201 ggggcggacg gcggcaccgc cgacctcgtc gcctcctacg ccgagccgct gcccctgcgg
     4261 gtgctgtgtg agaccgtcgg cctgccgtac gaggaccggg agatctacct gccgcacacc
     4321 ctcgcactcc tgggcgccgc cgggctgacg atggaggagg tgctcgccgc cctgtacgcc
     4381 ctgcaggact acgccgccga cctcatcgcc cgcaaggaac gcgacggcga cggcgaggac
     4441 tacatccggc tcctgctggc cctggcccgg cggccggaca gcgagatcac ccgcgacgac
     4501 gtggtgagct ttgtcgtcac catgctcatg gccggctaca agaccaacat ccagcacacg
     4561 ggcaacgccc tgctcgcgct gctcacccac cccgagcagt tgcgcaccct gcgtgcggcg
     4621 cccgagcgga ttcccaccgc ggtggaggaa ctgctgcgct acgtcccgtt gatgaacgcc
     4681 atcaacatcc tcgtggcgac cgaggacttc accctgcacg ggcagcacat ccgggccggt
     4741 gacgcggtcg tgcccgtacc ggcgtccgcc aaccgcgacc cggcggcgtt cgccgagccc
     4801 gaccgcctcg acctggcccg gacccccaac ccgcacatcg ccttcgggca cggcccgcac
     4861 gcgtgcaccg gcgggaacct gacccgtatg cagttgacca tcgcgatcca ggtgctgctg
     4921 gaacggctcc cggacctcga actcgccgtc ccggccgaga gcatcgactg ggacgagtcc
     4981 accccgctgc gcgcaccggc ccggcttccc gtgcgctggt gagaccggcc cgcccgggcc
     5041 ccgtgcccgg gcgggccctg tccgcgacat ccccacagca acgagaaaga ggctcatcct
     5101 ccgtgtccac accaccgcaa gcgcccgcgt ccggccaggc agcccacgag gagcccctcc
     5161 cgcgctaccc cttcagcacc cggggcgacc ggctcgcccc cgaactcgac gagctgcgcg
     5221 gccgctgccc cgtggcccgg gtgaacacca actccggtga acaggcgtgg ctggtgaccg
     5281 ggtacgaact ggcccggcag gtactgcggg accgttcctt cgcccgctcc gtgctgggcg
     5341 aggcggaccg ccccacccag gacgccccga tcctggcgcc cgagctgctc gacgccatga
     5401 accacctcca gcaggccggg ctgcgcgacg aagtgctcaa ggcgctcggc cgcgaccagc
     5461 ccgacctgcc cgccgactgg gtcgcggaga tcaccgccga ggggctggac accatggtcc
     5521 gggacggcag ccccggcgac ctccagcgcc acttcgcgga ctgggtcgcc gcccagtgca
     5581 tgtgccggct gctcgggctg cccttcgcgg accgcgactg gctcgccgta cgggccgacc
     5641 tggacctgac catggtcacc ccgacccccg aggaactcgc ccgcaactgg gaggagatcc
     5701 gcgcgtacat ggacgcgcac atgtccgacc ggcgccccgg cgagccgcgc ggcctcatcg
     5761 accgcctcgc cgacctcaac gccacccacg aagggctgac gcggcgccag ctctccaaca
     5821 tcgtggccgt cctgttcgtc agcggttacg aggacttcgc gagcttcctc ggtgtcgcgg
     5881 cgtacaacct gctccagcgg cccgaggtca tgagcgcggt gcgggccgag ccggagacga
     5941 tgccgcagtg cgtggaagag ctgctgcggt gcagcgtcgt cctgggcaac gcgattcccc
     6001 gcttcgtcac caaggacgcg gagatcgggc cggcccaggt gaaggagggc gagatgatcc
     6061 tgctctccct ggacgcggtc aactacgact ccgccgcctt cccggacccc aagtccttca
     6121 cccccgcccg cacccccaac ccgcatctgc gcttcggcta cggccgccac cactgccccg
     6181 gggcccactt ggtccgccgc cagtccgaag tcgccttccg cgtcctgctc gaccggctgc
     6241 ccggcatccg cctggccgta ccgccggccg aggtcccctg gcacccgaac cgcatggcca
     6301 tcatgccggc cgaggtcccg gtcacctggt gaccaggcgt gcccgacgag gtcgggctcg
     6361 gccgactcgt cgagcacgac cgacgatgta cattcgctcg ggccgggaac gtcagtgcag
     6421 cgaacgttgc ccgagtgaac ccagcggaag gacgactgtg cccaccaaga ccctgcagat
     6481 ccccaccccg gacggccagg ccgacgcgtt cgccgccttc cccgacgacg gcgagcggca
     6541 cccgggagtg ctgctgtacc cggacgcctt cggcgtacgg cccgagttgg agcggaaggc
     6601 ccgagaactg gccgggcacg ggtactacgt gcttgtcccc aacctctact accggcgtgg
     6661 cccggccccg gtgatcgacc tccccgagca catcggggaa gaggcacgcc ccgcggtctt
     6721 cgcccaggtg atgccgctga tggaggaaca caccaccgag cgggtcctgc gcgacgccga
     6781 ggcctacctc acgttcctca cctcccagcc cgaggtcggc cccgggccgg tcgccgtgct
     6841 gggctactgc ctgggcgccg tcctggcgat gcgtaccgcg accgcccacc ccggccgggt
     6901 ggccgccgtc gccggattcc accccgcccc cctggtcacc gacgcgcccg acagtccgca
     6961 ccgcctggtg tccgaactca ccgccgaggt ccacatcggt ctcgccgaag gcgatatgac
     7021 gcccgaggct ctcagcgagt tcacccgggc tctggacacc gccggggtcg cccacaccac
     7081 ggagacctac cccgacaccg tccacggctt caccatgtcc gacaccgcgg cctacagccc
     7141 cgccggactc cagcgccact gggaccgcct gcttccgctg ctggagcgca ccctgaccaa
     7201 gggctga
//
