LOCUS       BGC0002586             11905 bp    DNA     linear   BCT 06-APR-2020
DEFINITION  Lactiplantibacillus plantarum strain A-1 plasmid pA1_ASM1, complete
            sequence.
ACCESSION   BGC0002586
VERSION     BGC0002586
KEYWORDS    .
SOURCE      Lactiplantibacillus plantarum
  ORGANISM  Lactiplantibacillus plantarum
            Bacteria; Bacillota; Bacilli; Lactobacillales; Lactobacillaceae;
            Lactiplantibacillus.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 18:03:03
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..11905
                     /db_xref="taxon:1590"
                     /mol_type="genomic DNA"
                     /organism="Lactiplantibacillus plantarum"
                     /plasmid="pA1_ASM1"
                     /strain="A-1"
     protocluster    1..11905
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[5761:5956]"
                     /cutoff="20000"
                     /detection_rule="(glycocin or sublancin)"
                     /neighbourhood="10000"
                     /product="glycocin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5762..5956
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(glycocin or sublancin)"
                     /neighbourhood="10000"
                     /product="glycocin"
                     /protocluster_number="1"
     cand_cluster    1..11905
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(glycocin or sublancin)"
                     /kind="single"
                     /product="glycocin"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..11905
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0002586"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..11905
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="glycocin"
                     /region_number="1"
                     /rules="(glycocin or sublancin)"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            complement(212..568)
                     /gene="asmH"
     CDS             complement(212..568)
                     /codon_start=1
                     /gene="asmH"
                     /note="hypothetical protein; putative ASM1 immunity
                     protein"
                     /product="AsmH"
                     /protein_id="AOF43519.1"
                     /transl_table=11
                     /translation="MITKICVIPAVVTGIQESSISGNVIIDSAITAHFTHRLKKALHEI
                     SDTLKIFSLSEFEPDLTPDSNTLYVFSENVYHNLPASFLKNAKYVKFRNSQIFSSSLED
                     LAHAINIKLLQYRS"
     repeat_region   order(342..353,355..366)
                     /rpt_type=inverted
     gene            618..1904
                     /gene="asmA"
     CDS             618..1904
                     /codon_start=1
                     /gene="asmA"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_2"
                     /gene_kind="biosynthetic-additional"
                     /note="UDP-GlcNAc glycosyltransferase family 2"
                     /product="AsmA"
                     /protein_id="AOF43520.1"
                     /sec_met_domain="Glycos_transf_2 (E-value: 8e-25, bitscore:
                     77.4, seeds: 256, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKNKTTHTDTYLNNHLKSITDSFDFACVQNISPAKNDIALSYIVI
                     CKNSQSTIERCLNSIYKNMTARDELIVLDTGSTDRTVQLIEKTVPKVRILVTNWTNDFA
                     RARNIALKSASKDWVFFVDSDEWLDSEDGTSLEAIIKKAQTKKLNFVINPTFSDSTGQI
                     YQTVGRIFPTNASFHYYAKIHEEIRKNDKKLGYDVKHFVSADVIIYHDGYNKEVMQKKN
                     KIKRNISLLQEMVHEEPQNARWTYLLARDGFAVFSPAQVEQLIKKTLELVSADNRQKKY
                     SLVAQKLLGRILFKEGKTANALLSFQNVLKMTNGTDSDALYYITVIKINNLLLETNSIE
                     KELLNYLSSHDQTIDVNSDISGNYFHIAQIILECDLISANYEHLLPLISEIPKEFSNNI
                     KNKIKAASKIYSKLQGDSKDANNKTNRSK"
     misc_feature    738..1271
                     /gene="asmA"
                     /inference="profile:Pfam:PF00535"
                     /note="Region: glycosyl transferase family 2"
     misc_feature    order(984..986,990..992)
                     /gene="asmA"
                     /note="metal-binding site"
     misc_feature    1873..2283
                     /gene="asmB"
                     /inference="profile:Pfam:PF03412"
                     /note="Region: peptidase C39 family"
     gene            1873..3981
                     /gene="asmB"
     CDS             1873..3981
                     /codon_start=1
                     /gene="asmB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288:ABC
                     transporter related protein (Score: 158.2; E-value:
                     5.4e-48)"
                     /gene_kind="transport"
                     /note="C39-ABC exporter of bacteriocin ASM1"
                     /product="AsmB"
                     /protein_id="AOF43521.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 1.4e-19, bitscore:
                     60.0, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRIIKQIDQNDCGPAAVATMLGMLSKNRISDWQIKQIICPTRYGT
                     NFISIISGLKSVRIKSTVQQCVKDIAVFDEIEFPVLTQIMQHGYLHFVVITKCTRSKVY
                     WADPGIGKIESNYKSNFLTFWNPIILTIPKSQSYEFLKAKIKALKRPTLWLSFKKHWLA
                     LLIYWILSIIVFAITLFLATMYAAYFNRIVPNKFISAIPTMTSVYLTIQAAALLLEVIN
                     AILIAFTGNAISNELYKDIFTAFPHKKRSVIESFKDGEIITRFNAIYQIAGRQLVLLGD
                     LPIDIITMSITLFLLGRINPFLTILLLIPMILMSLLFYLSSDTIKKRSTNLFKSQEIFS
                     QDIIEQITNFSTIKTFHAGTYITSKITKSMEKYIIANRNFSLYDRLQQIARNGVSQFFS
                     IILFSVGAFLVIKGNIVLGTLLSFYALTGNVLNPFIKIITMQVNLSQGKVATERYLDLL
                     ISSEDNTNMINQLTADSIELNKVTFSYDSITPIINNLTGVFSKFPTAIIGNSGGGKSTI
                     AKLIAGFYPVDGLSIGGISYTKLGAKTISSTITYVEQSPQIFSDTIMNNITLGRKNITE
                     KSVYEIANKIGFSTFLKQLPDGLNTRLGSSGYTLSGGQKQLLNIIRSMIVPSKFIIFDE
                     ITNGLDINTKNKVENYLLSQNEVHCIFITHDLTLAMKCIDIFTVKNGKILNVTRTKHTV
                     KSLERTLL"
     misc_feature    2362..3174
                     /gene="asmB"
                     /inference="profile:Pfam:PF00664"
                     /note="Region: ABC transporter transmembrane region"
     misc_feature    3370..3918
                     /gene="asmB"
                     /inference="profile:Pfam:PF00005"
                     /note="Region: ATP-binding domain of ABC transporter"
     gene            3978..4397
                     /gene="asmC"
     CDS             3978..4397
                     /codon_start=1
                     /gene="asmC"
                     /note="putative thioredoxin domain protein"
                     /product="AsmC"
                     /protein_id="AOF43522.1"
                     /transl_table=11
                     /translation="MKKITIAIIAIIAVALIGAYSWKKLTSVQAPLYQTVKAKKLQSDI
                     KNKKNEIVYFYQKGCEGCEHSTPILNRFIRNKHVTIQAIDINADPSKDFILGTLGISST
                     PTVLFIRKGKITQRINQIFGYEDLTTKYNKFRGDF"
     misc_feature    3984..4046
                     /gene="asmC"
                     /note="transmembrane region"
     misc_difference 4059
                     /citation=[1]
                     /gene="asmC"
                     /note="conflict, replace t13 in GenBank accession AB474371
                     with G4059"
     misc_feature    4074..4379
                     /gene="asmC"
                     /inference="profile:Pfam:PF00085"
                     /note="Region: thioredoxin domain"
     misc_feature    order(4155..4157,4164..4166)
                     /gene="asmC"
                     /note="thioredoxin CXXC motif; active site"
     gene            4398..4931
                     /gene="asmD"
     CDS             4398..4931
                     /codon_start=1
                     /gene="asmD"
                     /note="putative thioredoxin domain protein"
                     /product="AsmD"
                     /protein_id="AOF43523.1"
                     /transl_table=11
                     /translation="MKTLIKNKTSLILLSLLIFSVVVGIFSLALTKHNSILHEQTLQSE
                     IRSGSTTNTLIYKDLNSITVPSFKKMVAEKNNFIVYIGRPTCSDCNLFDPILINELKKE
                     HMTTQVTFLNVAWERQKKWATWVQFKKNYGFEQTPALIHYRNGKVFSIIQWEQNNGISR
                     EALHLWLTNQKKTI"
     misc_feature    4428..4493
                     /gene="asmD"
                     /note="transmembrane region"
     misc_feature    4572..4916
                     /gene="asmD"
                     /inference="profile:Pfam:PF00085"
                     /note="Region: thioredoxin domain"
     misc_feature    order(4656..4658,4665..4667)
                     /gene="asmD"
                     /note="thioredoxin CXXC motif, active site"
     misc_difference 4840..4841
                     /citation=[1]
                     /gene="asmD"
                     /note="conflict; GenBank accession AB474371 contains an
                     extra base (a795) inserted between A4840 and G4841"
     misc_difference 4883..4884
                     /citation=[1]
                     /gene="asmD"
                     /note="conflict; GenBank accession AB474371 contains an
                     extra base (t839) inserted between T4883 and C4884"
     gene            4901..5680
                     /gene="asmE"
     CDS             4901..5680
                     /codon_start=1
                     /gene="asmE"
                     /note="putative LytTR response regulator"
                     /product="AsmE"
                     /protein_id="AOF43524.1"
                     /transl_table=11
                     /translation="MAYQSEKNNLIPQTRIFVISNQPGRQDFNKSLLQEMGNLSDIIKF
                     VHPIQLAHSTHEKYTDVNCSLSIIILNVDEEDNLLKKLEAIVEQLPLSIKYFIYVGPSK
                     KTLENLIRCHYMPYDALITKDFDYKTFTILAADIYNILKKSSLSLKDSDIITISVQRQK
                     YFLKLDDILFIITDSHPHRLIFHLKNTEYQTRMTLKQVYHQCKKLTVCHEGALVNLQNV
                     TKFNKHKRILYFENGDICEVSKRYIKSISEKIPMKTV"
     misc_difference 4967
                     /citation=[1]
                     /gene="asmE"
                     /note="conflict, g923 in GenBank accession AB474371 with
                     C4967"
     repeat_region   order(5149..5160,5164..5175)
                     /gene="asmE"
                     /rpt_type=inverted
     misc_feature    5381..5665
                     /gene="asmE"
                     /inference="profile:Pfam:PF04397"
                     /note="Region: LytTR DNA-binding domain"
     sig_peptide     5762..5824
                     /gene="asmF"
                     /gene_synonym="bactA1 (GenBank locus AB474371)"
                     /note="double glycine leader peptide"
     gene            5762..5956
                     /gene="asmF"
                     /gene_synonym="bactA1 (GenBank locus AB474371)"
     CDS             5762..5956
                     /codon_start=1
                     /gene="asmF"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     glycocin: glycocin"
                     /gene_kind="biosynthetic"
                     /gene_synonym="bactA1 (GenBank locus AB474371)"
                     /note="precursor of diglycosylated bacteriocin ASM1"
                     /product="AsmF"
                     /protein_id="AOF43525.1"
                     /sec_met_domain="glycocin (E-value: 4.7e-51, bitscore:
                     160.5, seeds: 2, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSKLVKTLTVDEISKIQTNGGKPAWCWYTLAMCGAGYDSGTCDYM
                     YSHCFGVKHSSGGGGSYHC"
     misc_feature    order(5837..5839,5906..5908)
                     /gene="asmF"
                     /gene_synonym="bactA1 (GenBank locus AB474371)"
                     /note="Cys5-Cys28 in mature (secreted) ASM1; disulfide
                     bond"
     misc_feature    order(5858..5860,5885..5887)
                     /gene="asmF"
                     /gene_synonym="bactA1 (GenBank locus AB474371)"
                     /note="Cys12-Cys21 in mature (secreted) ASM1; disulfide
                     bond"
     misc_feature    5876..5878
                     /gene="asmF"
                     /gene_synonym="bactA1 (GenBank locus AB474371)"
                     /note="GlcNAc moiety beta-O-linked to Ser18 in mature
                     (secreted) ASM1; glycosylation site"
     misc_feature    5951..5953
                     /experiment="NMR structure (PDB ID: 2MVI)"
                     /gene="asmF"
                     /gene_synonym="bactA1 (GenBank locus AB474371)"
                     /note="GlcNAc moiety S-linked to Cys43 in mature (secreted)
                     ASM1; glycosylation site"
     repeat_region   order(5979..5999,6001..6021)
                     /note="putative transcription terminator (asmF gene)"
                     /rpt_type=inverted
     repeat_region   6127..6144
                     /rpt_type=inverted
                     /rpt_type=tandem
     repeat_region   6433..6492
                     /note="One of two 60-bp imperfect direct repeats (the other
                     is 11435-11494) that separate the bacteriocinogenic region
                     and the replication/mobilisation region of pA1_ASM1"
                     /rpt_type=direct
                     /rpt_type=dispersed
     CDS             6487..6852
                     /codon_start=1
                     /inference="EXISTENCE: similar to AA
                     sequence:INSD:EPD25299.1"
                     /note="orf1"
                     /product="hypothetical protein"
                     /protein_id="AOF43526.1"
                     /transl_table=11
                     /translation="MQDTPETAEFAKRAEEASQRARKMKTELRKKRRADKLKLQQQLGA
                     ELMKETNIVSAQQLKQYLQDNLNYEAEAENQQDYQQALIELNTFANQLTWDEERGYWRV
                     ANLRTLTDWLAGFRTNN"
     gene            7309..7626
                     /gene="mob"
     CDS             7309..7626
                     /codon_start=1
                     /gene="mob"
                     /inference="EXISTENCE: similar to AA
                     sequence:INSD:EPD25296.1"
                     /product="putative mobilization protein"
                     /protein_id="AOF43527.1"
                     /transl_table=11
                     /translation="MTAQKKRTQINVRLDELELEKVKYSADVLGLSVGQYVKQVVTKSP
                     LIEPKFSPDFQKTAIRQLVGIANNLNQLTRLAHINGTTSTQQAIDQLRKEVDLLWQQLA
                     K"
     gene            7605..8936
                     /gene="mobA"
     CDS             7605..8936
                     /codon_start=1
                     /gene="mobA"
                     /inference="profile:Pfam:PF03432"
                     /product="relaxase superfamily protein"
                     /protein_id="AOF43528.1"
                     /transl_table=11
                     /translation="MATIGKITTASSGSAAIDYAYGRNKMPAATKDWLKQQGVDPATVE
                     QLQNRAVVMSGLNVDPEYAKTQMKATRMAFSTRGKQTTRVIQSFPTIDLDPADPHDWQT
                     ANQLGLELAKQAFPDYQVAVYTQLDGKGHKLHNHIIVNMPNIRTGEKYHEHQSWQRLSR
                     INDEISQEHNLSIIDRNTRAPERRTMAEWQLNAKNEYVWKDDLRRRIDKAILESSKNSY
                     KDFSEHLTQSGIIIHDRGKNFSYEFLDADKKHRRARGKTLGADYEKETILNELERQNRQ
                     PATELPRLIEPNRQIEQRKRAVESREPISESRKRTTAKSGQSVDSLTIAIGRTRQTART
                     VANNLNKQNSRKQRFRERITSFTKRFREIGHQINQFIQRLDCYNRTVNNFQQRLKADQQ
                     AKQQAFQSFQQRFEADQKVQKQHELEQQQLNKSFPIEEDHGIER"
     gene            8926..9360
                     /gene="mobB"
     CDS             8926..9360
                     /codon_start=1
                     /gene="mobB"
                     /inference="EXISTENCE: similar to AA
                     sequence:INSD:KRK08994.1"
                     /note="truncated lacks 80 AA C-terminal region of
                     homologues"
                     /product="putative mobilization protein"
                     /protein_id="AOF43529.1"
                     /transl_table=11
                     /translation="MNVNDREIITQIMDAYRNGTLDWANVPAFQAIIRQEIRKALTDSH
                     FQAQQQTQLKNDHTWYHLEIERAIEKTAEQVNTEQIKQTVEKDIAEQQHKLSLQLLNNM
                     NMQAKLEKQQKLWIPVLLTVLPTMAFLLISFCAIWFIFIR"
     CDS             9371..9811
                     /codon_start=1
                     /note="orf2"
                     /product="hypothetical protein"
                     /protein_id="AOF43530.1"
                     /transl_table=11
                     /translation="MPKYFKTYHLSRYLLLVVTLWCVLGVYGSFTVGRIHAHQVRVQRQ
                     EKRLKAKRQAIKQRKQVEMKRQVSKLQAQVNELQNQATSLNQRNKHIILPSDADEKLKS
                     KIVNQVNHLNPTIAELDTKFTGVPALQKSYRQLQHLTEGYGF"
     misc_feature    10102..10131
                     /note="associated with origin of replication; Region:
                     AT-rich 1"
     misc_feature    10170..10193
                     /note="associated with origin of replication; Region:
                     AT-rich 2"
     repeat_region   10241..10323
                     /note="22-bp direct tandem repeats (iterons) characteristic
                     of class A theta replicons"
                     /rpt_type=direct
                     /rpt_type=tandem
     gene            10418..11197
                     /gene="repB"
     CDS             10418..11197
                     /codon_start=1
                     /gene="repB"
                     /inference="profile:Pfam:PF01051"
                     /inference="similar to AA sequence (same
                     species):INSD:ABG23030.1"
                     /product="Rep3 superfamily initiator replication protein"
                     /protein_id="AOF43531.1"
                     /transl_table=11
                     /translation="MQGKISKKADVAFSSLLSRQDYLVVQGNDLAKAFGNLSAFEHQVL
                     DYCFSFVTQNSKAEDEYSVEAIDLIHHLGLNSSGHNYERIAKAFKALNEKTALYFHTES
                     DGVPGILMTQLFSHIKFFKNGHVTFQFSQDAAPYVFDLRSNYYSFKLSELSRVKSKYSL
                     IMLKLIEANRMGNQNPITIKGTLEDWQGWFLGADRRWPAGRFMQKAIDVAIDELYQKMG
                     LVFTVTTLKHKRSVVGYVIDVTKPEENRKIKKVIRGD"
     repeat_region   11435..11494
                     /note="One of two 60-bp imperfect direct repeats (the other
                     is 6433-6492) that separate the bacteriocinogenic region
                     and the replication/mobilisation region of pA1_ASM1"
                     /rpt_type=direct
                     /rpt_type=dispersed
ORIGIN
        1 gcagatatat cagctaatat tgaacaagct tatcatgatg ggattttatc tggaaaatca
       61 acgcaacgat tacaagatgg gatggttgtt agttcctatc taattgcgta aaaaaagaac
      121 ggttattatg tcaaaagctt atgcgaacag ctaaaactgc cgataagccg gccctacctg
      181 ctgaattgaa agtacaagct gtacactata tttaagatct atattgtaat aattttatat
      241 taattgcgtg ggctaaatct tcgaggcttg acgaaaagat ctgactatta cgaaatttca
      301 catattttgc gttttttaaa aaagaagccg gtaaattatg atatacgttt tctgagaaaa
      361 cgtataatgt gttggaatct ggggttaaat ccggttcaaa ttctgacaaa gaaaaaatct
      421 ttagagtatc tgaaatttca tgtaacgcct ttttcaatct atgggtaaaa tgagctgtga
      481 ttgctgaatc tattatcaca ttaccagata tgctactttc ttgaattcct gtcacaactg
      541 ctggtataac acaaatcttt gtaatcattt gttgcctctt ttcttaaata taaaatacca
      601 cggaagtagg tcttgaaatg aagaataaaa caactcatac cgacacttat ttaaataatc
      661 atttaaaatc tattacagat tcatttgatt ttgcatgtgt acaaaatata tctcctgcaa
      721 aaaatgacat tgctttatct tacattgtaa tctgtaagaa ttctcagtca actattgaaa
      781 gatgtttaaa ttcaatttac aaaaacatga ctgctagaga tgaattaata gttttagaca
      841 ctggctcgac cgatagaaca gttcagttga ttgagaaaac tgttccaaaa gttaggatat
      901 tagtaacaaa ctggacaaat gacttcgcaa gagccagaaa tatagcatta aaatcagctt
      961 caaaagactg ggtttttttt gttgatagcg acgaatggct cgatagcgaa gatgggacta
     1021 gcctagaagc aattataaaa aaagctcaaa caaaaaaact taatttcgtt attaatccaa
     1081 cattttccga tagtacggga caaatatatc aaactgttgg tcgtattttc ccgacaaatg
     1141 cgtcctttca ttattatgcg aaaattcatg aagagattcg aaagaacgat aaaaaactag
     1201 ggtatgatgt taagcatttc gtaagtgccg acgtgattat ctatcatgat ggctataaca
     1261 aagaagtaat gcagaagaaa aataaaatca agagaaacat tagtttatta caagaaatgg
     1321 ttcacgaaga accccaaaat gccaggtgga cgtatttatt agcacgcgat ggtttcgctg
     1381 tattctctcc tgctcaagtt gagcagctaa taaaaaaaac attagagtta gttagcgcag
     1441 ataaccgtca gaaaaaatat tcattggttg ctcaaaaact acttgggcgt attctattta
     1501 aagagggtaa aacagctaat gctctgctat cttttcaaaa tgttttaaaa atgaccaatg
     1561 gaacggatag tgatgcacta tattacatca cagttattaa aatcaacaat ctattgttgg
     1621 aaacaaactc tattgaaaaa gaactattga actatttaag ttcacacgat caaacaattg
     1681 atgtcaatag tgatatatcg ggaaactact ttcatattgc ccagattatt ttggaatgcg
     1741 acttaatcag cgccaactat gaacatcttt taccactaat ctcagaaatc ccaaaagaat
     1801 tctcaaataa tataaaaaat aaaatcaaag cagcatcaaa aatatattca aaattacaag
     1861 gtgacagcaa agatgcgaat aataaaacaa atagatcaaa atgactgcgg accagcagca
     1921 gttgcgacaa tgctgggtat gttaagcaag aatagaatat ctgattggca aataaaacaa
     1981 attatatgtc caacacgtta tggaaccaac tttattagca taatatctgg attaaagtcc
     2041 gttagaatta aatcaacagt acaacaatgc gtcaaagata ttgcagtttt tgatgaaatt
     2101 gaatttcctg tattaaccca aattatgcaa catggttact tacattttgt agtcatcaca
     2161 aaatgtactc gttccaaagt atattgggca gaccctggga tcggcaaaat tgagagtaat
     2221 tacaagtcta acttcctaac cttctggaat cccatcattt taacaatacc taaatcacag
     2281 agctatgaat ttcttaaagc caaaattaaa gcattgaaga gaccgactct gtggcttagt
     2341 ttcaaaaaac attggctagc tttattgatc tactggatac tttcaataat tgtatttgct
     2401 ataacacttt ttctagcaac tatgtatgct gcttatttca accgtatagt acctaataag
     2461 tttatttctg caattcctac catgacctct gtgtatttaa ccattcaggc cgccgctctt
     2521 ttactcgaag ttattaatgc aatactaatt gctttcacag gtaacgcaat tagtaatgag
     2581 ttatataaag atatctttac cgcttttcca cacaaaaagc gttcggtcat agagtctttc
     2641 aaagatggag aaattattac aagatttaat gccatatatc aaattgctgg tagacaattg
     2701 gtacttttgg gtgatcttcc aattgatatt atcaccatga gtataaccct ttttcttctt
     2761 ggtcgtatca atccattttt aacaattttg cttcttattc caatgatatt aatgtcgtta
     2821 ttattttatt taagctcgga cactatcaaa aaaaggagca caaatttatt taaatctcag
     2881 gaaattttca gtcaagacat tattgaacaa ataactaatt tttctactat aaaaaccttt
     2941 catgctggaa cctatatcac ttctaaaata acaaaaagta tggagaaata tattatagcc
     3001 aatcgtaact tcagcttgta cgatagatta caacaaatag ctcgaaatgg cgtttcacag
     3061 ttttttagca ttatattgtt ttcagttgga gcttttttag tcataaaagg gaatatcgtt
     3121 cttggaacat tgctcagttt ttacgcactg acaggtaacg ttctaaatcc cttcataaaa
     3181 ataataacta tgcaagtaaa cctttctcaa gggaaagtgg ctacagaacg ttacttagat
     3241 ttattgattt cttcagaaga caatacgaac atgataaatc agctaactgc agattccatc
     3301 gaactaaata aagtcacatt ttcatacgat agtattacac caataatcaa taatctcact
     3361 ggtgtttttt ctaagtttcc aacagccata attggcaata gtggaggtgg taaatcaacc
     3421 attgctaaac taatagcagg attttaccca gtagatggtc tgtcaatagg cggaatttcc
     3481 tatacaaaac tcggtgcaaa gacaataagc agtacaatta cctatgttga acaatcgcca
     3541 caaatatttt cggacacaat catgaataat ataacccttg gcagaaagaa tatcaccgaa
     3601 aagtcagttt atgaaatagc taacaaaatt ggatttagca cgtttttaaa gcaattgcca
     3661 gatgggctaa atacacgatt aggatcttct ggatatacat tatctggggg acaaaaacaa
     3721 ttattaaaca tcataagatc aatgatagtt ccaagtaaat ttattatttt cgacgaaatc
     3781 accaatgggt tggacattaa tacaaagaac aaagttgaaa actacttact atcacaaaat
     3841 gaagtccatt gtatatttat aactcacgat ttaacacttg ctatgaaatg tattgacatc
     3901 tttacagtaa agaatggaaa aattctaaat gtaacacgta ctaaacacac tgttaaatca
     3961 ttagagagga cattgttatg aaaaaaataa ctattgctat cattgctatt atcgctgttg
     4021 cactaatagg agcttattct tggaaaaagc ttacatcagt ccaggctcct ttataccaaa
     4081 cagtaaaagc aaaaaaacta caatctgata tcaaaaataa gaaaaatgaa attgtttact
     4141 tttatcaaaa aggttgtgaa ggctgtgagc atagtactcc gattctaaat cgttttattc
     4201 gaaataaaca tgtaactatc caagcaatcg atataaacgc agatccaagt aaagatttca
     4261 tcttgggaac attgggaata tcatcaacac caaccgtatt atttatccgc aaaggaaaaa
     4321 taactcaacg tataaatcaa atctttggat atgaagattt aacaactaaa tacaataaat
     4381 tccgaggtga tttttaaatg aaaacattaa taaaaaataa gacatcttta attttgttat
     4441 cacttctcat tttttcagtg gtcgtgggaa tcttttcatt agctctgaca aaacataact
     4501 ctattttaca tgaacaaaca ctccaatctg aaatcagatc gggttcaaca acaaacactc
     4561 taatttataa ggatttaaat tcaataacag tcccatcatt taaaaaaatg gttgccgaga
     4621 aaaataattt tattgtatat attggtcgcc ctacttgttc ggattgtaat ttattcgatc
     4681 ctattttaat taacgagtta aaaaaagaac atatgaccac tcaagttaca tttctcaatg
     4741 tcgcctggga acggcaaaaa aagtgggcaa catgggttca attcaaaaag aactatggat
     4801 ttgaacaaac acctgcttta attcactacc ggaatgggaa ggtattcagt attatccaat
     4861 gggaacagaa taacggaata agtcgggagg cattacactt atggcttacc aatcagaaaa
     4921 aaacaatcta attccacaaa ctaggatatt cgttatatca aaccagccag gtcgacaaga
     4981 ttttaataaa tcactgctac aagaaatggg aaatttgtct gatattatca aattcgttca
     5041 ccctatacag ttggcgcact caacccatga aaaatatact gatgttaatt gctcactttc
     5101 aattattatt ttgaatgtag atgaagaaga taatctatta aaaaaattag aggcaattgt
     5161 cgaacaattg cctctttcaa taaagtattt tatttacgtc ggaccaagta aaaaaactct
     5221 tgaaaatcta attagatgtc attatatgcc ttatgatgca ttaataacta aagattttga
     5281 ctataaaact ttcactatac tggctgctga tatttataat attcttaaaa aaagctcatt
     5341 gtccttaaaa gattcagata ttataacaat ttcggttcaa agacaaaaat actttttaaa
     5401 attagacgat attttgttta taataactga cagtcaccca catcgactaa tctttcattt
     5461 gaaaaacact gagtatcaaa caagaatgac tctaaagcag gtgtaccatc aatgcaaaaa
     5521 attaaccgtt tgtcatgaag gagcattagt gaatttacaa aatgtcacta agttcaacaa
     5581 gcacaaaaga attttatatt ttgaaaatgg tgatatttgt gaagtttcaa aacgatatat
     5641 caaatcaatt agtgagaaaa tcccgatgaa gacggtttag ttcccgatga agataagaac
     5701 tccaaatata agtgttttcg aattataatt gcactagtaa aaaatttagg aggttacgac
     5761 catgagtaaa ctagttaaaa cattaactgt cgatgaaatc tctaagattc aaaccaatgg
     5821 tggaaaacct gcatggtgtt ggtacacatt ggcaatgtgc ggtgctggtt atgattcagg
     5881 cacttgtgat tatatgtatt cacactgctt tggtgtaaaa cactctagcg gtggtggcgg
     5941 tagctaccat tgttagcctt aaaagaatat ttcgttataa gttggcttta tatttaactc
     6001 agtttaatat aaagccaact ttttaatact cttaaaattg ttgattaacg ttaggcttgt
     6061 agacttatct gaaatttaaa aacagcgtcc tttatcaagc ttccgctcat aatccattgt
     6121 ttgcatcgat tcaggcctga atcgtttgca ctctgccacc ccttgaccat gtcgttaaat
     6181 gcttggtttt atcaattaag ttaattagta acccacttga gagtatctag attttctgtc
     6241 gtcatctatt tatcttgcaa ataacacgac catttgtacc aggtgttagc atcagcgtgc
     6301 tgcaaggcaa cttatcgggg aagattactg atagtatcgt gttttacaac atgcaaactg
     6361 gcctctacaa cacttatcta aatcaaataa ctattaaacc taaaagacta aaagtgaaat
     6421 atgagcgcag ttatcaagaa tatgtgacta agaaaactaa actaatactg aaaggattgg
     6481 attaacatgc aagatacacc tgaaactgcc gagtttgcca aacgcgccga agaagctagt
     6541 caacgtgcaa gaaagatgaa aaccgagtta cgtaagaaac ggcgagctga taaattaaaa
     6601 ttacaacaac aactgggtgc tgaactaatg aaagagacta acattgtatc agctcaacaa
     6661 ctcaaacaat atctacaaga taacctgaat tatgaagctg aggctgagaa tcaacaagat
     6721 taccagcaag ctctaattga gttaaacacc tttgctaatc aattaacttg ggatgaagaa
     6781 cgaggctact ggcgagttgc taacctgcga acgttaaccg attggctagc aggctttagg
     6841 acaaacaatt aattattgta aagttggcaa acacgtcaac ctcacaataa ttattggttc
     6901 taatcactaa tttacgagta gtaaatttag tgatttaatt tttaccctta gtgccatcaa
     6961 gcagacattt gcgtaaagta aactgactgt tggtggcact ttttcggttt ccttttgcta
     7021 aaaggtaagg tgtgacgggg ttgactgttg gtggcgttgt gtgaagcaca atcgacacgg
     7081 ctttgacttt gggggggagt taagaggggg agcgttgcgc cctctcttac aagcgcaaga
     7141 gggagacaag gagcgtagcg atattgtcta cacattgccc cgcttgtcat acgactttaa
     7201 actgttataa tgatggcgtg aatagcgtca ttactagtta ccgtttctta gctaacatgc
     7261 aaccgcgtag ggcgcacgtt ggcttagcca aggaggtgtt tttgactttt gactgcacaa
     7321 aaaaagcgca cgcaaattaa cgtccgtctt gatgaacttg aattagaaaa agtgaaatat
     7381 tcagctgacg ttctcggtct gtctgtcggc caatatgtta aacaagttgt gacaaagtca
     7441 ccgttgatcg aacccaaatt ttcgcccgat tttcaaaaaa cagctattcg acaattggtc
     7501 ggtattgcta acaatttaaa ccagttaacc cgtttagctc acatcaatgg cactacttca
     7561 acccaacaag ctattgacca actacgaaag gaagttgacc tgctatggca acaattggca
     7621 aaataacgac tgctagtagt ggatcggcgg ctattgacta cgcttacggc cgcaataaaa
     7681 tgccagccgc aactaaagat tggcttaaac agcaaggtgt tgacccagct accgttgagc
     7741 aactgcaaaa tcgagcagtc gtcatgtctg gtctgaacgt tgaccctgaa tatgctaaaa
     7801 cccaaatgaa agccactaga atggctttta gcacccgtgg taaacaaacg actcgtgtta
     7861 ttcagtcctt cccaacgatt gacttagacc ctgctgatcc tcatgactgg cagacagcta
     7921 accaattagg cttagaactc gccaaacaag cttttcctga ttatcaagtt gctgtttata
     7981 cccagctaga cggcaaggga cacaagctcc acaaccacat tatcgttaat atgccgaata
     8041 ttagaaccgg tgaaaagtat catgaacatc aaagctggca acggttatct agaattaatg
     8101 acgaaattag tcaagaacat aacctatcaa tcattgaccg caataccaga gcccctgaac
     8161 gacgaaccat ggctgaatgg caactcaacg ccaaaaatga gtacgtctgg aaagatgatt
     8221 tacgtcgtcg gattgataaa gcaatattag agagttctaa aaactcatat aaggacttct
     8281 cagagcattt aactcaatct ggcataatta tccatgacag aggtaaaaat ttctcatacg
     8341 agtttttaga cgctgataaa aagcaccggc gtgctcgtgg taaaacttta ggtgcagatt
     8401 atgaaaagga gacgatactt aatgagttgg agcgacaaaa tcgacaacca gcaaccgagc
     8461 taccaagact tattgaacct aatcgacaaa ttgaacaacg aaaacgggct gttgaatcaa
     8521 gagaaccaat ctctgaatca agaaaacgaa caactgcaaa atcaggtcaa agtgttgaca
     8581 gtcttacaat cgcaattggc cgaactcgac aaacagctcg aacagtggca aacaatctca
     8641 acaaacaaaa cagccgaaaa caacgattta gagagcgaat tacaagcttt acaaaacgtt
     8701 tcagagaaat tggacaccaa attaaccagt ttatccaacg actagactgt tataaccgaa
     8761 ccgttaataa cttccaacaa cggctcaaag ctgaccagca agcgaaacaa caagcctttc
     8821 aaagtttcca gcagcgcttt gaggctgacc aaaaagttca aaaacaacat gagcttgaac
     8881 aacaacagct aaataaatca tttccaatcg aggaggatca tggtattgaa cgttaacgac
     8941 cgagaaatca tcactcaaat aatggacgcc tatcgtaatg gcacgcttga ttgggctaat
     9001 gtcccggctt ttcaagcaat tatcagacaa gaaatccgaa aagcactgac cgatagtcat
     9061 ttccaagcac aacaacaaac ccagttaaaa aatgaccaca cttggtatca tctagaaatc
     9121 gaacgggcca tcgaaaaaac cgccgagcaa gtgaatacgg agcaaattaa acaaaccgtc
     9181 gaaaaggata ttgccgagca acagcacaaa ttaagcctac aactacttaa taacatgaac
     9241 atgcaagcta aactagaaaa gcaacagaag ctttggatac cagtattgct gactgtctta
     9301 ccaaccatgg ccttcttgct aattagtttt tgtgctatct ggttcatctt catacgctag
     9361 gaggcttagt atgcctaaat acttcaaaac ctatcacttg tcacgatact tgttgttagt
     9421 agtcacacta tggtgcgtac taggtgtcta tggctcgttt accgttggcc gaatacatgc
     9481 gcaccaagta cgggttcaaa gacaagaaaa acgactaaaa gctaaacggc aagcgattaa
     9541 gcaacgcaaa caagttgaga tgaagcgtca agtctctaag ctacaagcac aagttaatga
     9601 actgcaaaat caagctacca gtctgaatca acgcaacaag catatcatac taccctctga
     9661 cgccgatgaa aaattaaaat cgaagatagt taatcaggtt aaccatctca atcctacaat
     9721 tgcagagctt gatactaagt tcactggtgt tcctgcctta cagaagagct atcggcagtt
     9781 acaacatctt acagaggggt atggatttta gactatgtcg ctgttatcaa gatatatatg
     9841 gcattttaga acactacggt ttacatgttc ttaccccttg atacgactag tatcttgaat
     9901 agtaaggact acctcagctc ctagtcgtgg ttaactctgc ctgagggcgt gcccgaagtt
     9961 tccctaacaa cgggcttgat gttgacccta gcaacgacca acgggaggcg cagttaaatg
    10021 tgagcgtgtt ttgcgctcac tcctttttag cgaggggggg aactggggac ggcgttacgt
    10081 cccctagaca gtcttcaatc gttaattttt ttaagattct taaaattttt tgttcgcgcg
    10141 cgcgttaatt tagttacttt cagacatact tttaattact tttaaatact ttaggagcct
    10201 tagaaatggc ttaaccacgc gggttacagg cgtcgaaaat acctttttgt acgtgatatg
    10261 taaccttttt gtacgtgata tgtaaccttt ttgtacgtga tatgtaacct ttttgtacgt
    10321 gattattata taagattgca taatatccta attaagggta ttattctgtt tgtatttata
    10381 aatgactttt ttgtacgttc ctttaggagg tgatttaatg caagggaaaa tttccaagaa
    10441 agctgatgtt gctttttcaa gtttattatc acgtcaagat tatttagtcg ttcaaggtaa
    10501 cgacttagct aaagcatttg gcaacttgtc ggcttttgaa catcaggtac tagactattg
    10561 ttttagcttt gtcactcaaa acagtaaagc agaagatgaa tactctgtgg aagctattga
    10621 cttgattcat catcttggtt tgaactcttc aggacacaat tatgagcgta tcgccaaggc
    10681 ttttaaggct ttaaatgaga aaactgcctt atactttcac actgaaagtg atggcgtacc
    10741 gggaatcctc atgacccaat tattcagtca tatcaagttc tttaagaacg ggcatgttac
    10801 ctttcaattc tcgcaagacg cggccccgta tgtgtttgat ttacgttcaa attactattc
    10861 attcaagctt agtgagttgt cgcgtgtcaa atccaagtac agccttatca tgttaaagct
    10921 cattgaagcc aatcgaatgg gaaatcagaa cccaattacg ataaaaggta ctttagaaga
    10981 ttggcaaggc tggtttctcg gtgctgaccg ccgatggcca gctggccgat ttatgcaaaa
    11041 ggcaattgac gtagcaattg atgaactcta tcaaaagatg ggactagtct ttaccgttac
    11101 taccttaaag cataaacgtt cagtagtagg ctatgtgatt gatgtgacca aaccagaaga
    11161 aaatagaaaa ataaaaaaag tcatcagggg cgactgaaac cgcaatccct gatgacttaa
    11221 gctctggcaa ctacttacaa tagttacttt agcgaactta gcaaagagca ttacctacct
    11281 ttcttgctgg ataggggtaa tgctccaacc accggacaag ccggcttctg tattatagca
    11341 tgtaccggtt tgtcctcaac attaggagga taaaaccatg aaccaaaata ctttagcttt
    11401 caaacaaact gccaaacacg ttctgagcaa aaacatcaaa aatatatggc taggaaagct
    11461 aaactaatac tgaaaggatt gagataatat gcaattctta gaaatcagag ctaacggaca
    11521 ttacgcatgg attagcgcca atgacccaag tcaatcgtca cttaataatt ccatcgaaca
    11581 ccacaggtta actggttaaa cgagatacca gagaaacaaa cgtggcaagc ttatatgaat
    11641 accagcaaca atcctgcccc agcacgtcgt catgttctcc aacaacgagc cacaatttgg
    11701 ctagatatct tctaactgtg aacctatcac ctacctataa gtatgataag atcatagtgt
    11761 aaggggcgtc gcgaaagggc aataggatat cttttactga ggaggataca aaatgaaatt
    11821 caaacaatga ctgttacctg ttatcatttc actgataagt ttgtcgttgc ttattttaat
    11881 aacaagttat actactgttg ttcat
//
