LOCUS       BGC0002602             15136 bp    DNA     linear   PLN 17-DEC-2018
DEFINITION  Aspergillus flavus culture SRRC:0537 aspergillic acid gene cluster,
            complete sequence.
ACCESSION   BGC0002602
VERSION     BGC0002602
KEYWORDS    .
SOURCE      Aspergillus flavus
  ORGANISM  Aspergillus flavus
            Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina;
            Eurotiomycetes; Eurotiomycetidae; Eurotiales; Aspergillaceae;
            Aspergillus; Aspergillus subgen. Circumdati.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 18:04:26
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 15136
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..15136
                     /culture_collection="SRRC:0537"
                     /db_xref="taxon:5059"
                     /mol_type="genomic DNA"
                     /organism="Aspergillus flavus"
     misc_feature    1..15136
                     /note="aspergillic acid gene cluster"
     protocluster    1..15136
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[4677:7743]"
                     /cutoff="0"
                     /detection_rule="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX))"
                     /neighbourhood="20000"
                     /product="NRPS-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      4678..7743
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="0"
                     /detection_rule="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX))"
                     /neighbourhood="20000"
                     /product="NRPS-like"
                     /protocluster_number="1"
     cand_cluster    1..15136
                     /SMILES="NC(CC(C)C)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX))"
                     /kind="single"
                     /product="NRPS-like"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..15136
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0002602"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..15136
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS-like"
                     /region_number="1"
                     /rules="cds((PP-binding or NAD_binding_4) and (AMP-binding
                     or A-OX))"
                     /subregion_numbers="1"
                     /tool="antismash"
     mRNA            complement(join(<407..1347,1400..>1475))
                     /product="aspergillic acid biosynthesis Ankyrin domain
                     protein, putative (AsaA)"
     CDS             complement(join(407..1347,1400..1475))
                     /codon_start=1
                     /note="11571_g"
                     /product="aspergillic acid biosynthesis Ankyrin domain
                     protein, putative (AsaA)"
                     /protein_id="AZL87942.1"
                     /transl_table=11
                     /translation="MGAPHEEIQALKRRREQNRLAQRRRRDNVRRRLRDLGLDTGSPAS
                     ARQTSLCSSRDSRVTLNSHQSLRSTDSLSLETPNSDTEMSPYDPPLQSKLRLDSQIPLA
                     EISFPSYASSVSPSSSAGRLSSSPSPSQRPFIDSTDLTSLQSVYNLTSLAVHLDESSMP
                     CGEADIPILQDSNFPRTPNLESLMSGCNDPSAHQPWILTSSTVGEQMSSQALPHSPGPQ
                     HCSTPLPAETRPRWTTALHMAVSQGNFSVMRLLLSYGADPNAVNSEGATALHVGVMNGN
                     HTMVAELLQRGADPTLTNAAGWLPLHQAVHAGDEGCVRVLLEADQPVDYPISDFDYT"
     mRNA            join(<3168..3445,3545..>4163)
                     /product="GA4 family aspergillic acid hydroxylase (AsaB)"
     CDS             join(3168..3445,3545..4163)
                     /codon_start=1
                     /note="11570_g"
                     /product="GA4 family aspergillic acid hydroxylase (AsaB)"
                     /protein_id="AZL87943.1"
                     /transl_table=11
                     /translation="MRFRQQVETCLNYWPAEGPVQKELILGTVGAYRRPIDSRPVLIQD
                     VRGQEGTFTLDIHGFQFIKHISQHVASFDEAYVKTHMYPEAESILKNVTGATRAHVFSH
                     ITRTAPYESVEAMADSADPDAKATSVMVPARHVHVDQSESGAFEVLKDNMTAVEAERLL
                     KTRWAIVNIWRPLKPVPRDPLAVSDAGSFHDEDLLEIYGRVPGKQVKKDYDAATKGSGF
                     GMLYGKYSPGQKWFYMSDMKPDEALLIKCYDSRDDGRTARRTPHTAFVDPRTRDVKEAR
                     ESLELRCLVFFEDQPLV"
     mRNA            complement(<4678..>7743)
                     /product="aspergillic acid non-ribosomal peptide
                     sythetase-like (NRPS-like) (AsaC)"
     CDS             complement(4678..7743)
                     /NRPS_PKS="Domain: AMP-binding (16-418). E-value: 8.2e-87.
                     Score: 283.2. Matches aSDomain:
                     nrpspksdomains_AZL87944.1_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (536-601). E-value: 8.1e-17. Score:
                     53.1. Matches aSDomain: nrpspksdomains_AZL87944.1_PCP.1"
                     /NRPS_PKS="Domain: TD (643-889). E-value: 3.1e-64. Score:
                     208.4. Matches aSDomain: nrpspksdomains_AZL87944.1_TD.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRPS-like: PP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRPS-like: AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRPS-like: NAD_binding_4"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002:AMP-dependent synthetase and ligase (Score: 310;
                     E-value: 4.1e-94)"
                     /gene_kind="biosynthetic"
                     /note="11569_g"
                     /product="aspergillic acid non-ribosomal peptide
                     sythetase-like (NRPS-like) (AsaC)"
                     /protein_id="AZL87944.1"
                     /sec_met_domain="AMP-binding (E-value: 9.5e-94, bitscore:
                     302.9, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 7.3e-16, bitscore:
                     47.3, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="NAD_binding_4 (E-value: 8.7e-63, bitscore:
                     200.4, seeds: 102, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSIFSSISSLGLEACYRHHVRTSPNATAVVDGDQSMTYRELDTRV
                     NDLASILGRENIEEEEPIGILVPMGITHVVAQAAVLRLGGSCVPMDLSFPDQRINDLLR
                     ALKTRIVLTVESEKARFAEFQTILVDSKYANLHQNGYHEDKIPAVETGRNHRTHILHTS
                     GTTGLPKPVEIMSKGITRMAFNTQCVEFKSTDRVAQISAPSFDAALFEIWTTLARGAAI
                     VLLPKNVVIDPVALHDSLRKYRITSILVTTALLNHVVSAIPNAFEDLDYVLTGGEAANP
                     SVMQVILENGPPKKLVHAYGPTECTIITTYHLTTLEEVRRGQTPIGRPLDNTTVYILDD
                     NLRPVKEGIVGELYIGGDAVARGYLGRPEANAKSFLEVSHLSKDGSPVRIYRSGDLVRM
                     LDTGAIEFVARADNMVKIRGFRIEPAEIEGALLKSEMVQGTVVLPVHRPGKETYIVAFV
                     IPKHDGAFSLEQLDEYLRRRLPAYMMPRLEAVASLPLTVHGKIDRVAVMKKHMEETKRA
                     EQQVLISSNVKDAGDSVTWLRTLWTSVLGISNIDNETSFFHLGGSSLQAAALLVHIRRR
                     FGLTLTMQQIYDSPTLLGLASVIDAGHAKSKVDHSRLGIFIADSQLAKDIPVLSKEAPD
                     WRSPSEGKVFLTGATGFLGTYFLRELIDRPDVRNVQCLVRASDAHSARARLLGALEKYG
                     LGWADNLDKVTAIAGDLGKDLFGLSETEFHELALWTSVIFHVGAHVNYVQPYEKHRNTN
                     VYGTLNCIKLATTGRTKALHYTSTAAVTGPVSHFTGADKIPEDVDLGEFQGWLPYDIGY
                     TQSKWVSEQLIHSMIAKGLPAIVFRPGFIMGDSLRGKGNCDDFMCRVFIGSIKLGYRPI
                     LPNQSKIMIPVDFITTALLHITSNPYNFGQTFHLVPQTPEEDTDIETSWNMLKELGYDL
                     KAVEYKDWLEILSKDKDLLTNPLLPMLPVLQEPVRKHLTRWELYEDMATYDVTNTRRSL
                     ADRGNLKSGIGLEDLRRHVEDWVARGLVPSRN"
     aSDomain        complement(5077..5814)
                     /aSDomain="TD"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AZL87944.1_TD.1"
                     /evalue="3.10E-64"
                     /label="AZL87944.1_TD.1"
                     /locus_tag="AZL87944.1"
                     /protein_end="889"
                     /protein_start="643"
                     /score="208.4"
                     /tool="antismash"
                     /translation="VFLTGATGFLGTYFLRELIDRPDVRNVQCLVRASDAHSARARLLG
                     ALEKYGLGWADNLDKVTAIAGDLGKDLFGLSETEFHELALWTSVIFHVGAHVNYVQPYE
                     KHRNTNVYGTLNCIKLATTGRTKALHYTSTAAVTGPVSHFTGADKIPEDVDLGEFQGWL
                     PYDIGYTQSKWVSEQLIHSMIAKGLPAIVFRPGFIMGDSLRGKGNCDDFMCRVFIGSIK
                     LGYRPILPNQSKIMIPVDFITTAL"
     aSModule        complement(5077..7695)
                     /complete
                     /domains="nrpspksdomains_AZL87944.1_AMP-binding.1"
                     /domains="nrpspksdomains_AZL87944.1_PCP.1"
                     /domains="nrpspksdomains_AZL87944.1_TD.1"
                     /final_module
                     /locus_tags="AZL87944.1"
                     /monomer_pairings="Leu -> Leu"
                     /starter_module
                     /tool="antismash"
                     /type="nrps"
     aSDomain        complement(5941..6135)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AZL87944.1_PCP.1"
                     /evalue="8.10E-17"
                     /label="AZL87944.1_PCP.1"
                     /locus_tag="AZL87944.1"
                     /protein_end="601"
                     /protein_start="536"
                     /score="53.1"
                     /tool="antismash"
                     /translation="LRTLWTSVLGISNIDNETSFFHLGGSSLQAAALLVHIRRRFGLTL
                     TMQQIYDSPTLLGLASVIDA"
     CDS_motif       complement(6460..6525)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AZL87944.1_0003"
                     /evalue="3.80E-10"
                     /label="NRPS-A_a8"
                     /locus_tag="AZL87944.1"
                     /protein_end="428"
                     /protein_start="406"
                     /score="31.9"
                     /tool="antismash"
                     /translation="FVARADNMVKIRGFRIEPAEIE"
     aSDomain        complement(6490..7695)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AZL87944.1_AMP-binding.1"
                     /evalue="8.20E-87"
                     /label="AZL87944.1_AMP-binding.1"
                     /locus_tag="AZL87944.1"
                     /protein_end="418"
                     /protein_start="16"
                     /score="283.2"
                     /specificity="substrate consensus: Leu"
                     /tool="antismash"
                     /translation="RHHVRTSPNATAVVDGDQSMTYRELDTRVNDLASILGRENIEEEE
                     PIGILVPMGITHVVAQAAVLRLGGSCVPMDLSFPDQRINDLLRALKTRIVLTVESEKAR
                     FAEFQTILVDSKYANLHQNGYHEDKIPAVETGRNHRTHILHTSGTTGLPKPVEIMSKGI
                     TRMAFNTQCVEFKSTDRVAQISAPSFDAALFEIWTTLARGAAIVLLPKNVVIDPVALHD
                     SLRKYRITSILVTTALLNHVVSAIPNAFEDLDYVLTGGEAANPSVMQVILENGPPKKLV
                     HAYGPTECTIITTYHLTTLEEVRRGQTPIGRPLDNTTVYILDDNLRPVKEGIVGELYIG
                     GDAVARGYLGRPEANAKSFLEVSHLSKDGSPVRIYRSGDLVRMLDTGAIEFVARADNMV
                     KIR"
     CDS_motif       complement(6619..6702)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AZL87944.1_0002"
                     /evalue="3.40E-14"
                     /label="NRPS-A_a6"
                     /locus_tag="AZL87944.1"
                     /protein_end="375"
                     /protein_start="347"
                     /score="44.9"
                     /tool="antismash"
                     /translation="GIVGELYIGGDAVARGYLGRPEANAKSF"
     CDS_motif       complement(6817..6846)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AZL87944.1_0001"
                     /evalue="4.00E-03"
                     /label="NRPS-A_a5"
                     /locus_tag="AZL87944.1"
                     /protein_end="309"
                     /protein_start="299"
                     /score="9.9"
                     /tool="antismash"
                     /translation="YGPTECTIIT"
     mRNA            join(<8333..8503,8564..8751,8807..>10007)
                     /product="deoxyaspergillic acid cytochrome P450 oxidase
                     (AsaD)"
     CDS             join(8333..8503,8564..8751,8807..10007)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1034:cytochrome P450 (Score: 187.1; E-value: 9.2e-57)"
                     /gene_kind="biosynthetic-additional"
                     /note="11568_g"
                     /product="deoxyaspergillic acid cytochrome P450 oxidase
                     (AsaD)"
                     /protein_id="AZL87945.1"
                     /sec_met_domain="p450 (E-value: 1.2e-56, bitscore: 180.9,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPSVPNLGLSIQNGLTKPEMLYPFLFGIFAVASLCIATLLFPASF
                     SAASRAISWVLSIYLELKNPIRHTETGRNIPGPSYVWPNGQGDVEKYVQGRTRSEQWQR
                     KYGNVYRIWAGMTPEVVLTRPEQLHAIFKDSDKHTKATNSDSGYFMSRILGQCLGLMAG
                     PRWKLLKGIAAPPFMHPTAVRSIGRIQEHVRAHFHDLETDGNLREGRIHPVQDLKMLPF
                     FIVAEANYGSLTPAMKSELDSLAPARENLMKFVLFGGLARFNISRFFPTEANRQLRRFR
                     SQWRAFNRAAYERAREKHPSAMVVQMYDAVHKGVLTEEQVAQTMDETLYANLDVTTGGL
                     SWNLVFLAANPACQARLHDEISALTPAEEEGYISRNGTYLAACVLESSRLRPALPFTIP
                     QSAPTERVVDGYRIPAGTNYVVDTWGLNVRDEFWAPDNSTYRPERFLNSSNTDLRYHFW
                     RFGFGPRQCIGRYTADVVIRAILLHLVKHYELQMLEEGDFTQDPECWITHPDLQVKCVR
                     RT"
     mRNA            join(<10378..11196,11249..>12334)
                     /product="aspergillic acid biosynthesis C6 transcription
                     factor, putative (AsaR)"
     CDS             join(10378..11196,11249..12334)
                     /codon_start=1
                     /note="11567_g"
                     /product="aspergillic acid biosynthesis C6 transcription
                     factor, putative (AsaR)"
                     /protein_id="AZL87946.1"
                     /transl_table=11
                     /translation="MERSCTQCHQRKVRCSKTLPCSACVRLGATCRFPTTDANAHRPRR
                     VQKITITDRIAQLERSLTILANSAGPLQGPENPNVRDTSSTLLPEPPQSPNRAQEVLVP
                     DGASTRYINETFLSQILDKEKALYKVIGTPRETEEITSGLRPEGLLASSRRPTHWSNEL
                     ELSRWQSAQLWQIYRNNVDPVVKILHLPTVEPLVYATMNGDGSDDCRALLFAIYFAAVT
                     SLSEVDAANLLGRDRRSCLLDFQARIEKVVIDAACLDAPTMLSLQALAIYITCLRAHRT
                     SRSGWIMNGVLIRAAQSIGLHRDGTHFNLTPFDAEVRRRLWWQIIVLDYRAAEDHGLAV
                     HGFGSRSDTRLPLHVNDSDLSPELRILPGPRAKWTEMSLFLLTSEIAIAFQKVYHTTVD
                     HTDNAQRLQTVQELTAYLESTYLCHCDTNIPIQKVAWLSTRSLLSKFEFFVYQLSLNNE
                     QSQEAVSSAAERTLISACSCLEQSMELQTDDLLRGFRWLFASYNQFHCLMYVLWHLCAQ
                     PTGPHVVRVWNIVDLVFNVTENDPTRPDPGPTWKVLHHLREKAAQRRGNVAMSSPPQAH
                     GPSDERVPESRTAEQHSGQDLEGTGDGFGDLLAPPLDPSSLTEWINLSENLGMYRFEP"
     mRNA            join(<12933..13137,13199..13305,13366..13540,13591..>14432)
                     /product="aspergillic acid MFS transporter, putative
                     (AsaE)"
     CDS             join(12933..13137,13199..13305,13366..13540,13591..14432)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1137:Major
                     facilitator superfamily MFS 1 (Score: 374.6; E-value:
                     8.2e-114)"
                     /gene_kind="transport"
                     /note="11566_g"
                     /product="aspergillic acid MFS transporter, putative
                     (AsaE)"
                     /protein_id="AZL87947.1"
                     /transl_table=11
                     /translation="MDRSRTSSQDRDVLPPRGNEGRISPSLDKEKSPGPENQPDAPPDG
                     GLTAWLVVVGAWCTSFCSFGWVNSVGIFQNYYESHLLKHLSSSTISWIPSLQIFFMFAM
                     GPIVGKLYDTFGARYLIIGGTFFHVFGLMMASISTQYYQLLLSQGICSAIGAAAIFQPA
                     LSAVSAWFNRKRGIAFATLSTGSSVGGVIFPIMVDRLVAKVGFGWSMRISAFMILFLLG
                     IAIVTVKARRPPQQGPKPSGVQLLQPFKEPVFIVTLLGYMLLTYGVFIPINYVIVQAVA
                     SGMNADLASYLVPMLNGASLFGRLGAGFMSDRYGKYNIFIVMCIVAGVLVLALWIPASS
                     NAPIIVFATLFGFASGAYVSLSPALIAQISPLKEVGYRTGLLFLFASVGGLTTSPIAGA
                     ILQNAGGDYTHMKIFSGVMLLGGTAFIITARIVGTGLKLVVKY"
ORIGIN
        1 gtcgtatcag ataagatagt cggcgcctcg gagggacttc aaccaccgag ccttggcgtt
       61 acgaaaggca tcctgcaagc atccgagttt gggaagtaaa atgttctaga cccgagacta
      121 agcgaattac tctcctggaa ggcagcaatc aaatgtaacc agtccgtagt ggcgtctggg
      181 atgtatttaa ccaggaccaa gcatggaatt ggagtagtga acatgggttg gaatccctca
      241 gtaggctagg cttgggggag cattcgtcat taggtaaaat aaaagggaaa aagagggaaa
      301 ctacaacaag aaacagacga aaagaaagga atagaaaaaa aattcatttc cactcttcat
      361 tgggaggcga acgtggatat catgtgacca gcaaccatgt agccagtcaa gtataatcaa
      421 aatcagagat aggataatca acaggctggt cggcctcaag caacacccgt acacacccct
      481 catcacccgc atgaacggcc tgatgcagtg gaagccagcc cgccgcattt gtaagagtgg
      541 gatcagcccc ccgctggagg agctcggcca ccatcgtgtg atttccattc atcaccccaa
      601 catgaagagc cgtagcccct tcgctgttga ccgcgttggg gtctgctcca tagctcagca
      661 aaagacgcat caccgagaag ttgccctgcg agacggccat atgtagcgcc gtggtccatc
      721 gtgggcgtgt ctcggccggt aatggcgtgc tgcaatgttg tgggcctgga ctgtggggaa
      781 gagcttgaga gctcatttgt tcaccaaccg ttgacgaggt taatatccag ggctgatggg
      841 ccgatggatc attgcagcca gacatcagac tctctaggtt tggagttcga gggaaattgc
      901 tgtcttgcag aatagggata tcagcctcac cgcacggcat actactttca tcgaggtgca
      961 ccgcgagtga tgttagatta tagacagact gcaaggaagt gagatctgtt gaatcaatga
     1021 atgggcgctg actgggagac ggcgaacttg agagtcggcc tgctgacgat gatggcgaga
     1081 cagacgaggc gtatgacggg aatgagatct cggcgagtgg aatttggcta tctagccgta
     1141 gtttgctttg gagaggtgga tcgtatggcg acatctctgt gtcagaattg ggtgtttcga
     1201 gcgataggga gtccgttgat ctgaggctct gatgagaatt tagcgtgact ctactgtctc
     1261 ttgatgagca aagtgacgtt tgacgggcag aggctggact gccagtgtcg agacccagat
     1321 ctctcaaccg ccttcggaca ttatcgccta ggtgaggtta gctgggcatt atagaaggta
     1381 ttcgagggtt gaaacgtacg ccgacgtctc tgtgcgagtc ggttttgctc ccggcgtctt
     1441 ttcagggctt gtatttcttc gtgaggcgcg cccatgttgg ctacagccgt gtacaacgag
     1501 agatctgtgt ctgatcctct cggtgactgc actgggtgga catccggaaa tatatagagc
     1561 aaggaagcac caaaactgga agttgcagcg caatcaacgg ctggtgccga gaaaggattt
     1621 tttacacatt tctaaaaagg gaaacaccag ctgccaagtt tcagtgagaa aataatacag
     1681 taggccataa ggtaatcctg aagggccttg ttatagaagg gagtgtaata aagtacaacg
     1741 gggaggtgga aagtactggc cgcagaggat tctgaaggga cttcagtgga ccaatggaat
     1801 acagctgaac agccacagaa aggctggcct cgtatcatcc ccttggctca gtggcacctc
     1861 gtattaagct gcaactcttc aacgaacgtc tcacggaggc tttccgggag gagccgcttc
     1921 cctctgcttg ttcactgctt agccaaagta tcatatagtc ttgtcaagac agccatgata
     1981 tatgtgtcca gttgccaaac taacgcttgg ccgaggaaca ctcaatcgga aatgaaaggc
     2041 agggcagcac aaggtaaagc ccgacatata tagacagtgc gacagtggac catgcgtgtt
     2101 catccactcg gcgcacattt gcctccgata ctacaatccc tgtctttact atgcggattg
     2161 cgtgaagaat gtctgtgatc tgcggtgggg aacaaacctc atgtccccac cgactagact
     2221 cggactccgt tccagcaggc tcttccgacg gccgagacaa accgaaacct ttggcctatt
     2281 gtcatgttgt gttgttcaac atggtagcag atatgggaaa gttcccagct ctgggtgttt
     2341 gacagactcg tattattggt tcattacata tacaagttca tttatgaatt ggaatgatta
     2401 aactgcgaca tttacaatcc tgtttcatca tgttaagtaa gcaacgtctt ctgtttgagc
     2461 caggacatgg tatgagttgt cgcagagact gtcctccccg ggatggagag agactcgcac
     2521 ttgcagtatg atcgatcaga catcagccga tgatcatctc tacacaagca atagccattg
     2581 acgccatgga acatgccaag acgacttatc tgatgatcat catcctgcat gtcatgcctc
     2641 tcatccctta tagcacagtt cgcattcgat cctatcattc cacgccactc gatcatcaag
     2701 catgaatatg agtttcaaga gacaaattgt gacgttatcg agatgtatat ttcgctctgg
     2761 accaatcgct cggtattctc ggagcgacga tcactgcctc atcaccctga agctgaatag
     2821 accatgtcgt atttatgcaa cgtgatcctt gaacgttaca tctccaaagc aactggcaac
     2881 caactcggcg tctcggtgta caacacagtc gccatcggtt tacgatcaag agtacaccgt
     2941 cggattgcga gtgcgaattg attcaggttg ccggaggtct gcgccatgac cccatcaagc
     3001 atttaaaggg tccctttggc atcgtcgaag aaagatggca taccaaccac atcattcacc
     3061 ctttatcact gaacagtgaa atatcggcat ccctagactc tgtcgactgc acgtcaatcc
     3121 caggacttcg gcttgcttcc cctagcttga ttgctccggc cataaccatg cgctttcgtc
     3181 aacaagttga gacctgcttg aactattggc ccgcagaagg ccctgtgcaa aaggagctta
     3241 tactgggcac agttggtgca tatcgccgac ccattgatag tcgacccgtc ttaatccaag
     3301 atgtgcgtgg ccaagaggga acattcaccc tggacatcca tggctttcag ttcatcaaac
     3361 atatcagcca gcatgtcgcg tcctttgatg aagcttatgt gaagacacat atgtaccccg
     3421 aagcggagag tatcctgaag aacgtgtaag tatagctttt cagtctatgt tcattccacc
     3481 ttgccccttt caggtcagcc tctattatac gtctataagg cccctgctca cgtgtctcat
     3541 aaagaactgg tgcgacccgc gcccacgtct tttcgcacat tactcgcact gctccctacg
     3601 aaagcgtaga agcaatggcc gattccgccg acccggatgc gaaagcgacg agtgtgatgg
     3661 tacctgcgcg ccatgtccat gtggatcagt cagagtccgg ggctttcgag gttctgaagg
     3721 acaacatgac tgcggtggaa gccgagcgct tgttgaagac ccggtgggct atcgtcaaca
     3781 tatggcgccc actgaagccg gttcctcggg accctttggc tgtatcggat gccggttcct
     3841 tccatgatga ggatctcctt gagatttatg gacgggttcc tggcaagcaa gtgaagaaag
     3901 actacgatgc cgcgacgaag ggcagtggtt ttggtatgct ttacggcaaa tattcacctg
     3961 gccagaagtg gttttatatg tccgacatga agccagacga ggcgctgttg atcaaatgtt
     4021 acgattcgag ggatgatggc cggacagcta gaagaacgcc tcatacggcc tttgtggatc
     4081 caagaacgcg ggatgtgaag gaagctcgcg agagcttgga attgagatgt ctagtcttct
     4141 ttgaagacca gccactggtc taaatgcaag tgatgtagta ccctttggca cgctgacgac
     4201 ggggatcatg tggttatgag aaagacagag gtactctaag agcttggaca ttgcgataaa
     4261 gtcaagtaca tgaaatgtca ctacatgtta tcgaggtcgc gtttacgtgg cacctcatgt
     4321 acctttgcgg gtcatacatg tacatataag tcacaaaagg atcaacataa cgccagggat
     4381 ggataatgtg gctctaagat ctcagcccgt tacctatacg ctctcctatt acaatcgcag
     4441 agaacatcca ttgctcgttt aaatcgttgc taaaaaagcg tagaggttgt ttaagcgctt
     4501 ataagacgat agtcttcatt ctccttcgca actctctttc ctttactagc tagtagttac
     4561 tgttttatga gccattctac aggtgcacgt ttttccttct acaaccagag tatatttaag
     4621 ctagataaag acagtgatct ccacaggtgg tcctaccaca tatgccatgt agaggtatta
     4681 gttcctcgaa ggcactagtc ctcgagcgac ccaatcctca acatgtcgac gtaagtcctc
     4741 cagcccaatg ccggacttca gatttccacg gtctgctagg gaccttctgg tatttgtcac
     4801 atcgtaagtg gccatgtcct cgtaaagctc ccatcgcgtc agatgcttcc gaactggctc
     4861 ctgcagtact ggtaacattg gcaacaatgg gttggtaagc agatccttgt ccttcgataa
     4921 tatctccaac caatccttgt attcaacggc tttcaggtca taccctagtt ccttgagcat
     4981 gttccaagag gtttcaatgt ccgtgtcctc ctcgggcgtt tgtgggacca ggtggaaagt
     5041 ctgcccgaag ttgtagggat tcgaggtgat atgcagcaga gcggtcgtga taaaatcaac
     5101 aggaatcatg atcttgcttt gattcgggag aatgggacga tatcccaact tgatagagcc
     5161 aataaacacc cggcacatga agtcgtcaca gttccccttg ccacgaagac tgtcacccat
     5221 gatgaagcct ggccggaata caatcgccgg taggcccttt gcgatcatag aatggatcaa
     5281 ttgctccgat acccacttgc tctgcgtgta accgatatca tagggcagcc agccctggaa
     5341 ctcaccaagg tctacatcct ctgggatctt gtctgcgcct gtgaagtgcg acactggccc
     5401 tgtgacggcg gccgtcgatg tataatgaag tgccttggtt cgtccggtcg tggcaagctt
     5461 gatgcagttc agagttccat agacgttagt gttacggtgc ttctcgtacg gttggacgta
     5521 gtttacatgc gcgccgacgt gaaagatgac gctagtccag agagcgagtt catggaactc
     5581 ggtctcggac agtccgaaca aatccttgcc cagatctccc gcaatagctg tcaccttgtc
     5641 cagattgtcc gcccatccca gcccgtactt ttccaaagcg ccgagaagtc gcgcccgggc
     5701 agaatgcgcg tccgaagcac gaactaggca ctggacgttt ctgacatccg ggcggtcgat
     5761 caactcacgc aagaagtacg tcccaaggaa tccagttgct ccagtcagaa acaccttacc
     5821 ttcggagggg cttctccaat ccggggcttc tttggacaga acaggaatgt ccttcgctaa
     5881 ctgggagtcg gcgatgaaga ttccaagccg ggagtggtcg actttgcttt tggcatgacc
     5941 agcatcaatc acgctggcca ggccaagcag ggtgggagag tcgtagattt gctgcatggt
     6001 caatgtcagc ccgaagcgcc gacgaatgtg gaccagcagc gcggctgcct gtagtgagct
     6061 gcctcccaag tggaagaaac tggtctcgtt atcaatgttg ctaatgccga gcactgatgt
     6121 ccacaacgta cgtaaccagg ttacactgtc cccggcgtct ttgacgttgg aggatataag
     6181 aacctgttgt tcggcccttt ttgtttcttc catatgcttc ttcatgactg ccacacgatc
     6241 gatcttacca tgaacggtca atggtagcga ggcgacagcc tccaaccggg gcatcatgta
     6301 cgccggtagc cgccgccgca gatattcgtc gagctgctcc agagaaaatg caccatcatg
     6361 tttgggaatt acaaaagcca cgatatacgt ttcttttccc gggcgatgaa caggcagcac
     6421 aactgtaccc tggaccatct cgctcttcag gagtgctccc tcgatctccg ctggctcgat
     6481 gcggaatcca cggatcttga ccatgttgtc tgcacgagcc acaaactcaa tggcgcccgt
     6541 gtcaagcatg cgtactagat cgcccgatcg gtagatgcga accggtgacc catccttcga
     6601 taaatgggac acttcgagga agctcttggc atttgcttct gggcgcccca gataaccgcg
     6661 tgcaactgcg tcgccaccga tatagagttc gccgacaatg ccttccttga caggccggag
     6721 gttatcatcc aggatataga ccgtggtatt gtccaatggt ctcccaatag gggtttgccc
     6781 ccggcgaacc tcctccaacg tcgtgaggtg gtaggttgtg atgatggtgc attcggtagg
     6841 tccgtaggcg tggacaagct tcttcggtgg gccattctcc aagatcactt gcatgacact
     6901 gggattggcc gcctcgccgc cggtgagcac ataatccagg tcctcaaaag cattgggaat
     6961 ggccgacacg acatggttca atagtgcagt cgtcaccaga atggacgtga tacggtattt
     7021 gcgcaggctg tcatgaaggg cgacaggatc aataacgacg ttctttggca ggagaacaat
     7081 ggcggctcct cgagcaagag tggtccatat ctcaaacaga gcggcatcaa aactgggggc
     7141 tgaaatctgc gcaactcgat ccgtgctttt gaactcaaca cactgcgtgt tgaacgccat
     7201 tcgtgtgatc ccctttgaca tgatctccac gggcttgggc agacctgttg tcccagaagt
     7261 gtgcaggata tgagtccggt gattgcgccc ggtctcgacg gcggggatct tgtcttcatg
     7321 atatccattt tgatggaggt tggcatactt ggaatccacg aggatcgttt ggaactcggc
     7381 aaagcgagcc ttctcactct cgactgtgag aacaatgcgc gttttcaagg cgcgtaaaag
     7441 gtcattgatg cgctggtcgg ggaatgagag atccatgggc acacaagatc ctcccaagcg
     7501 cagcacagcc gcttgggcta cgacatgtgt aatgcccatc ggcacaagaa tgccaattgg
     7561 ctcctcctct tcaatattct cccgacctag aattgaggcc aaatcgttga cacgggtatc
     7621 gagctcccga tatgtcatcg attggtctcc gtccactacc gcagtagcgt tgggagaggt
     7681 gcgtacatga tgccggtaac atgcctcgag gccaagtgat gagatgctgg aaaagattga
     7741 cattgtgagt gtggtttgtc agggagcaga atcaacctgg agatatgata aggtcgaatg
     7801 attgtgatgg acttcgctac ataccattac ttgggcttct gacctgaatt tatactctct
     7861 acaactgtac tttggcctca tttgcgtact cttcctgtat tactttcctc ctctccacaa
     7921 acttctcgat gacctgcaac aatcatcctt cgccatgtcc agttgtgggt ccttagccct
     7981 atgcttcata cacaatccta gccaatctga ctactcgctt ggcaagctaa attccagggc
     8041 tcggcgtcga agggtctcac attttgaaac tttatatacg agtcaggtca ggtcatgtca
     8101 tgtcatgtca tgtcagcaag tctctctgtg atcatattcg ctcattctag gcagccatat
     8161 gtacgaacat caattgtcca ttaccccatc atttcgcatc tcagacctac atccattttt
     8221 tctgcgtctg ttactactat ataggctgaa gggaccacga acttccggtt gtgtctaacg
     8281 cacctctcgg gttgggagag atcatagtac aacaagttcc tagactccaa ccatgccttc
     8341 cgtgccaaac ctgggccttt ccattcagaa tgggttaaca aagccggaaa tgttataccc
     8401 gtttctcttt ggcatctttg ccgtcgccag tctctgcatt gcaacattgc tcttcccagc
     8461 gtccttctct gctgcttcta gagctatctc ctgggtcctc agcgtatgta acatatccca
     8521 cgcctactct acatatagct caactgaccc ggttgggata tagatctatt tagagctaaa
     8581 gaacccgatt cggcatacag agaccggtcg gaacattccc ggaccgtcat atgtgtggcc
     8641 gaatgggcaa ggtgatgtag agaagtatgt tcaggggaga accaggagtg aacaatggca
     8701 aaggaaatat ggcaacgtct accgcatttg ggcgggaatg acccccgaag tgttcgtata
     8761 gcctcgtgtc atcggacaaa cgacaatctg actgacagac aactagggta ttgacgagac
     8821 ccgagcaact tcatgcgatc tttaaagatt cagacaaaca tacgaaagcc acgaacagtg
     8881 attctggcta cttcatgagc cgtatcctag ggcagtgctt ggggctcatg gcgggtccgc
     8941 gctggaagct tttaaagggg attgcggcac cacctttcat gcacccaaca gctgtcagat
     9001 ccatcggtcg aattcaagaa catgttcggg cccatttcca tgatcttgaa acagacggga
     9061 atctcagaga aggccggata caccccgttc aggacctgaa gatgcttccg tttttcatcg
     9121 ttgcggaggc caactatggc tccctgacgc cagccatgaa atccgaactc gattccctgg
     9181 cgccggctcg agaaaatctc atgaaattcg ttcttttcgg tggcctcgcc cgatttaata
     9241 tctctcgctt ctttcccacg gaagccaacc gtcaattgcg gcgattccgc tctcaatggc
     9301 gggcttttaa ccgcgccgca tacgagcgtg cccgggagaa acacccgtcg gcgatggtcg
     9361 tccagatgta cgacgccgtc cacaagggcg tcctcacgga ggaacaggta gcacaaacga
     9421 tggacgagac tctgtacgcc aacctggacg tcacaacggg gggtctgtcc tggaatctgg
     9481 tgttcttggc tgccaacccg gcctgccaag cacgactcca cgacgaaata tctgccctga
     9541 cacctgccga ggaagaagga tatatctctc gtaatggaac ctatcttgca gcatgtgtgt
     9601 tagagtcctc ccggctccgt ccagctctcc cgtttaccat tccgcaatcg gcgccgacag
     9661 agcgtgtagt cgatgggtat cgcatccctg ctggaaccaa ttatgtagtc gatacatggg
     9721 gactgaacgt gcgagatgag ttctgggcgc ccgataatag cacatatcgc ccagagaggt
     9781 ttctaaacag ctccaacacg gatctacgat accacttctg gcggtttgga tttggccctc
     9841 gccagtgcat aggccgctac actgcggacg tggtgatcag ggccatttta ctgcatctgg
     9901 tcaagcatta tgagttgcag atgctggagg aaggggattt cacgcaggac cctgagtgtt
     9961 ggatcacgca tccagattta caggtgaagt gtgttcggcg aacctgatga gtgtggcgag
    10021 ttgtaaaata tgtgggtttc ctcggctatc tttagaccat tagtgaagca caaaatatat
    10081 cttgttatca ccatgactac aggcctaact aggattcgtg atgagagtct taacttaggg
    10141 taacatcgac caaagagatt ccattatgta atatatagct caagaccttg gatgatcccc
    10201 gatccatgtc aatctatgat tctcgtatct gataaccaat tcctgcagct cggtgccttg
    10261 gctgcttggc acgaccgagc caccgagcac gacctctacg acgtccagca gctttccgtt
    10321 gagaatttta gacacacccc agcgttatat ccatttttgt actttaccca ccagactatg
    10381 gagcgatcat gcacccagtg tcatcagcgc aaagttcgat gtagcaagac gctgccctgc
    10441 tcggcctgcg tgagactcgg ggcgacgtgt cgattcccca ccaccgacgc caacgctcat
    10501 cggcctcgga gggttcaaaa aatcaccatc accgatcgga ttgctcaact ggaacggagt
    10561 ctgacgattt tagcaaacag tgctggaccc ttgcaaggtc ctgagaatcc gaacgtccga
    10621 gacacgtcct caacgttact gccagaacca ccgcaatctc ccaatcgcgc acaggaggtt
    10681 cttgttccgg atggtgcttc gactcgctac atcaatgaaa cattcctgtc gcagatcctg
    10741 gacaaagaaa aggcccttta caaagtcata ggtactccac gggagacgga ggaaatcacc
    10801 tcaggtttgc gaccggaagg cctgttggca agctccagac gccctacgca ttggagtaac
    10861 gagttggagc tttcgcgctg gcagtccgca cagttatggc agatctatcg caacaatgtc
    10921 gatcccgtag tcaaaatcct tcacctaccg acggtggagc cgctggtgta tgcgaccatg
    10981 aatggcgacg gttcggacga ctgcagggct cttctgtttg ccatttactt tgctgctgtg
    11041 accagcctgt ccgaggtcga cgccgcaaac ctcctcggtc gcgatagacg atcctgctta
    11101 ctggacttcc aagcccgtat tgaaaaggtc gtgatcgacg ccgcctgtct ggacgcgccg
    11161 accatgttgt ccctgcaggc cctagcaatc tatatcgtga gattagcctg tcccatccct
    11221 gcgcaatcga actgacttct gttacgagac ttgcctccgc gcacaccgga caagtcgatc
    11281 tgggtggatc atgaacgggg tcctcatccg tgctgcccag tccataggtc tccaccgcga
    11341 cggaacgcat ttcaatctca cccctttcga cgccgaggtg cgccggcgtc tctggtggca
    11401 gatcatcgtg ttggactatc gggctgccga ggaccatggt ctcgcggtac atggctttgg
    11461 cagccgatca gatactcgtc tccccctgca cgtcaacgat agtgatctat cgcccgaact
    11521 gcgaatcctg ccgggacccc gtgcgaaatg gactgaaatg agcctctttc tcctcacatc
    11581 ggagatcgcc atcgcgtttc aaaaggtata ccacactact gtggaccaca cagacaatgc
    11641 ccagcgactc caaactgtcc aggaactgac agcctacctg gaaagtacct acctgtgtca
    11701 ctgcgatacg aacattccca tccagaaggt tgcgtggctg tccacgcgct ctcttctttc
    11761 caagtttgag tttttcgtct accagctcag cctcaataac gaacaatcgc aggaggcggt
    11821 ttcctccgcc gccgagcgaa cactgatatc ggcatgttcc tgcttggaac aaagcatgga
    11881 actccaaacc gacgacctgc tacgtgggtt ccgctggtta ttcgcgagct acaaccagtt
    11941 ccactgcttg atgtatgttc tatggcacct gtgcgcacag cccaccgggc ctcacgtggt
    12001 tcgagtgtgg aatattgtcg acctcgtctt taatgtgacc gagaatgacc caactcgacc
    12061 tgatccgggg ccgacgtgga aggtgctgca tcacttgcgg gaaaaggcag cacaacggag
    12121 agggaacgtc gcgatgtcct ccccgcctca ggctcatggc ccgtctgacg aacgtgtccc
    12181 ggagtcacgg acggctgaac agcattcagg acaggatttg gaaggcacag gagacggatt
    12241 cggggatctc ttggctcctc cgctggatcc aagctccttg accgagtgga ttaacctttc
    12301 ggagaacttg ggaatgtatc ggtttgagcc gtagattgga aatgtatgtc attattgagg
    12361 gtgatagtag ttttaatgag tttatgaaat ctatcttgtg cactagaagc agcaaggctc
    12421 ccatcgatac gattcccagc gcaccctaca gatatatatc tctgaatgta gagctagtat
    12481 actagtagca cgacttccaa gagattaagc atccatagag gtgtcgacag agaaattgca
    12541 agaatgatct actcctgaag gagcctctgc ctacgtaagt ttgtatctaa gatccggtag
    12601 caccgttgca acctattcta caatggcacg aatatgacga cacatacgtg acgcacgtac
    12661 tagttcctat tgccccttat gcacaggggc ccagccgaag acgagcccta ctggtaatcg
    12721 ccacttggac tctcgaggcg tcggcttggt gtccctctgt cgccgagccg agctgatcgc
    12781 caaggaaccc cgaagccgag atgacttcac taccatgacc gcgataaaca aggcgccaaa
    12841 atatgtcatc cctatataac tgctggcttg accagagcat cctgaatgag cgcatcattc
    12901 attcctctgg ttaacacaga ctggccgaca acatggatcg gagccgtacc agttcccaag
    12961 accgtgatgt ccttccgcca cggggtaacg aggggcgcat ttcaccatca ctggacaagg
    13021 agaaatctcc gggtcctgaa aatcagccgg atgcacctcc tgatggggga ttgactgcgt
    13081 ggctggtggt ggtaggtgcg tggtgcacgt ctttctgcag cttcggatgg gtcaatagtg
    13141 agtgtctctc cacgatcatg aaatatctta gaagacagca tcactaatcg tataccaggt
    13201 gtcgggatat tccagaatta ttacgagtct cacctactga agcatctctc ttccagtacc
    13261 atctcctgga ttccatcctt gcaaatcttc ttcatgtttg ctatggtatg tcagcatcac
    13321 gaaccggtat actttatcca gtttccatat tcacagacca cccagggccc tattgttggg
    13381 aagctgtatg acacatttgg cgctcgatac ctgattatcg gagggacatt tttccatgtc
    13441 ttcggtctaa tgatggcgtc aatctcaaca caatactacc aactcttgct ctctcaggga
    13501 atctgcagtg ccatcggagc ggccgctata tttcaaccag gtaatatctt tttccttccc
    13561 tcgattcgcg gtggagactg accaaactag ctctaagcgc cgtaagcgct tggtttaatc
    13621 gcaaacgggg cattgctttt gctactctat cgacaggctc cagcgtcggt ggggtgatct
    13681 tccctattat ggtcgaccgt ctcgtcgcca aagtcggatt tggctggagc atgcgaattt
    13741 ccgccttcat gatcttgttc ctgcttggga tcgccatcgt gaccgtcaag gcacggcgtc
    13801 ccccacagca gggcccaaaa ccttccggtg tgcagttact gcaaccattc aaggagcccg
    13861 tgttcatcgt taccttgctg ggatacatgc tgcttaccta tggcgtcttt atccccatca
    13921 actatgtcat cgtccaggcg gtcgcaagcg ggatgaacgc cgacctcgca tcctacttgg
    13981 tccccatgtt gaacggggcc agcctttttg gccgcctcgg agctggattt atgtccgatc
    14041 ggtacggtaa atacaatatc ttcattgtca tgtgtatcgt cgctggcgtg ctcgtacttg
    14101 ctctttggat cccggctagc tccaatgcgc ctatcatcgt gtttgcgact ctctttggct
    14161 ttgcatctgg agcgtatgtg agcctttcgc ccgcgctgat cgctcagatc tcacccttga
    14221 aagaggtcgg gtatcgcact ggtctgctat tcttgtttgc atcggtgggt gggctgacga
    14281 caagtccaat tgccggagcg atcctgcaga acgctggtgg agactatact catatgaaga
    14341 tcttctcggg ggtgatgctc ttaggaggaa cggcattcat cattaccgcg cggatagttg
    14401 ggacaggtct gaaattggtg gtgaagtatt agatcgattc cgactgtggg gtgaggctgt
    14461 taaaattcaa acgaagcctc tagcttgaac tgtattctgt cctgtcccgc atctctcgtg
    14521 tttgaaggct tccagcaaag attccagcag gttttgcgag accaaaagat cctatgtttg
    14581 gtgttctatg tcattagcgc tagtgttaat agactaatta atcattcaat cttaatgtca
    14641 taatccgatg tttgcaggaa ctgctatcac tatgtagaaa ggcacgtaca aggacacata
    14701 attagccctc cgctaccaag tctgaaaggt atacgaggac agggatgggt gaattaaacg
    14761 tatcatatcg gcagcgtggg gttattaggt cggtgggata tcgaagccac acataatcta
    14821 tatcacaccg cgagtcgata gtcgtcatcc acgaacatcc tcccacgata cggagaaaac
    14881 atggcacaat ccatgctgca gcctcacaac atggcttaac tcgcgaaaag agtccgttta
    14941 ggaagtaaaa tcgccagtcc gaccggcact aactttggag atatcacaaa aatcgataga
    15001 ctgatagaaa aggggtagaa tacaaagttc ttcaggcgca tcctgccaaa cggatagttt
    15061 caagcccttg ccgctcacca ggaagcttgt gagcaatacg gagtaacgcg gcccaagcaa
    15121 tgactgtgga ttccca
//
