LOCUS       BGC0002609             18619 bp    DNA     linear   PLN 05-OCT-2016
DEFINITION  Aspergillus welwitschiae strain ITEM 7468 ochratoxin biosynthetic
            gene cluster, complete sequence.
ACCESSION   BGC0002609
VERSION     BGC0002609
KEYWORDS    .
SOURCE      Aspergillus welwitschiae
  ORGANISM  Aspergillus welwitschiae
            Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina;
            Eurotiomycetes; Eurotiomycetidae; Eurotiales; Aspergillaceae;
            Aspergillus.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 18:05:07
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..18619
                     /db_xref="taxon:1341132"
                     /mol_type="genomic DNA"
                     /organism="Aspergillus welwitschiae"
                     /strain="ITEM 7468"
     misc_feature    1..18619
                     /note="ochratoxin biosynthetic gene cluster"
     subregion       1..18619
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0002609"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..18619
                     /contig_edge="True"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            <250..>1025
                     /gene="ASPWELWI_7468_1151230"
     mRNA            join(<250..314,362..>1025)
                     /gene="ASPWELWI_7468_1151230"
                     /product="hypothetical protein"
     CDS             join(250..314,362..1025)
                     /codon_start=1
                     /gene="ASPWELWI_7468_1151230"
                     /product="hypothetical protein"
                     /protein_id="AOS89728.1"
                     /transl_table=11
                     /translation="MLLATPKSFRNNIGPLIAIVKIAMLSFACIYDMQPIWDCLYSESV
                     RSSSLLRNLVSRAALGNGGSLFQKQTMDGILDVYEAVSRLDQFFHADRSSTSSIECEWA
                     HILARSSPTTPWTDRHNYLTSSEKLNACCHLSCSIFWTTLRRRQLHDEQAPGSTDQDQL
                     NQVLLNYILQVEPLYWIQNAPEAFTWVAFTGAAASTQPGLRANFINHAATVITAIDGED
                     LTLIRQGWRYFGLIKRLGGA"
     gene            complement(<1080..>2495)
                     /gene="ASPWELWI_7468_1068681"
     mRNA            complement(join(<1080..1353,1414..1706,1832..1885,
                     1946..1985,2095..>2495))
                     /gene="ASPWELWI_7468_1068681"
                     /product="putative short chain dehydrogenase/reductase"
     CDS             complement(join(1080..1353,1414..1706,1832..1885,
                     1946..1985,2095..2495))
                     /codon_start=1
                     /gene="ASPWELWI_7468_1068681"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001:short-chain dehydrogenase/reductase SDR (Score:
                     110.6; E-value: 1.5e-33)"
                     /gene_kind="biosynthetic-additional"
                     /product="putative short chain dehydrogenase/reductase"
                     /protein_id="AOS89729.1"
                     /transl_table=11
                     /translation="MGKDLLSAIAIPELTGKVVIVTGGHTGLYVDSVGSNNNHLLLFGF
                     PLVLVIERTGSSSLVPLLTLSSASGFGTSIELARHGARVYIASRSQTRFEHARRDIIAE
                     CSEREVDVRFLNLDLSSMSSVRHAAEQFIRQESRLHLLVNNAGIMCVPYDETRDGFEMQ
                     LAVNYIGHFLFTSLLLPTLQQTAAEAADKGSVRIVNVSSDGHAKLAPKEGIIFSDMNMK
                     AFSVWARYGHSKLANVLHSRELAKRYPDILALSVHPGTVKTGLAAGPISSTPLYRLIKP
                     LVELGAPGPRKGAASILFAAVSPQLKLECHNGAYLLPIGRLSAPSKAGSDAKMAEELWE
                     WTVNALSHVKVDE"
     gene            complement(<2954..>4066)
                     /gene="ASPWELWI_7468_1120344"
     mRNA            complement(<2954..>4066)
                     /gene="ASPWELWI_7468_1120344"
                     /product="putative medium chain dehydrogenase/reductase"
     CDS             complement(2954..4066)
                     /codon_start=1
                     /gene="ASPWELWI_7468_1120344"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_zinc_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1040:alcohol dehydrogenase (Score: 111.3; E-value:
                     7.6e-34)"
                     /gene_kind="biosynthetic-additional"
                     /product="putative medium chain dehydrogenase/reductase"
                     /protein_id="AOS89730.1"
                     /sec_met_domain="ADH_N (E-value: 5e-09, bitscore: 24.8,
                     seeds: 66, tool: rule-based-clusters)"
                     /sec_met_domain="ADH_zinc_N (E-value: 1.6e-12, bitscore:
                     36.3, seeds: 86, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTHKAAVIHARDKPLVVENVPRPVASTGDAIVRILAADIVPYMR
                     EVITNQRPYPLSIPMTPGNTAIARIHEVGPDCVTLTPGQLVFCDITIRARDNPAVTMLY
                     GIHGAIHPAAQKLMDGVWRNGTYAEYTRFPLENLYPLDEEVLLERLGYSITDLCLLTAY
                     LVPFGGLAEVDVKPGEVVIVAPATGRFGGGAVGAALAMGATVIAAGRNRAALDKLEHIH
                     GTQRLTTVQITGDEASDTQSFQTAVGHPDGANVYLDLSPPTIKDSSLLVAGIRALGSFG
                     RCVLMGGHLGNIQLPYGEIMFKGIRIQGRFMYDRPHVFRLIQMVRSGLLPLGEKGGVTH
                     TEEFGLDEIERALDAAGKLSGWGGQVVLKP"
     gene            <6817..>10115
                     /gene="ASPWELWI_7468_1173614"
     mRNA            join(<6817..7372,7434..7510,7583..7896,7949..>10115)
                     /gene="ASPWELWI_7468_1173614"
                     /product="putative nitric oxide synthase"
     CDS             join(6817..7372,7434..7510,7583..7896,7949..10115)
                     /codon_start=1
                     /gene="ASPWELWI_7468_1173614"
                     /product="putative nitric oxide synthase"
                     /protein_id="AOS89731.1"
                     /transl_table=11
                     /translation="MPSTEINHTRGATPSPRCPFVEYERILEKNPILAPTGCTDKFCQA
                     GRLIHSDEPKVGENRPLEVVQNEAVAFLWQLWQDGVYTEDCYLERRTQVLEEIARSAKQ
                     TIVWEQESKRVDTTSIWTQSPEELRYGLRLAWRNSRKCIMRSRYQELELCDLRHIHTSV
                     GMATALLEEVTKAFNHGQIKPTVFVFPPKSVDGSGPMFWNKQLLNFAGYELEDGSIMGD
                     PSNVKLTQDIVDLGWTPPRAKTRWDLLPIVAMAENDAPALVEVPDDLRRLIEIQHPDYP
                     SFNELGLMWYQFPALSRLGFDIGGVQYTATPFIGWYMDAEIGVRNLADTFRFDALPEVV
                     KAIGFDIDAYRAKSEYAEVQELEDLPDYEQLRWQSRAQAELNYAVRWSFLQQGVTCVGT
                     LAASADWCQFDDEHAAKKGYRLNSDPYWLSPPQGSIVPLWHRGGAPNYQPKPMICRNRY
                     DPVNSWQRRKGSTDADPVLLIRKGNEWVTKPLTGFLADIPSRKRRKLTESQREEKPSTV
                     HIYYCSQGTTARTLAERLHRRVKRSARGLHVTFGSLNALDLTQIEASDIILLVASTTGS
                     GAFPANGADFAKAMGHFCESLKTDFSRVRLSVFGVGDSAYPNFNAAAITAYNLFHQLNV
                     LPIAGGLIKADVASETLPLRMFNHWSDAVESALVGGKGELSSDPEESFVSHAEMLRNFG
                     RATVISKTCSGADDGLITLTLDGIECADMAHLRLLPTNSASHVARAITALGISDPQLLM
                     PFSSGALQQCSYGIFFTRFVDLEDRGKLPSWAIESNNNSGHEKESILERLEQFSVSLQP
                     SQLSLELRHKICFGCPLLRPRAFSVASSSRYIGEKKVELLIKPHRRGRFSDIYLSTLQP
                     GGSIEYAIVPASPASFLPAVQKPLVVISTGSGFAPVRSLLQQRIQIAREWGEESSTPHP
                     FHTSPISLFAGFRLVDESIIHAVVDLAERYRLLDLACLAPSNPEKKRVQGYLLDKRDTI
                     RAKLVEQEGYVYVCGSAAMTEGVRVNLSEILAGEVKQIMGERYVEEVF"
     gene            <11839..>12747
                     /gene="ASPWELWI_7468_1086206"
     mRNA            join(<11839..12100,12194..>12747)
                     /gene="ASPWELWI_7468_1086206"
                     /product="hypothetical protein"
     CDS             join(11839..12100,12194..12747)
                     /codon_start=1
                     /gene="ASPWELWI_7468_1086206"
                     /product="hypothetical protein"
                     /protein_id="AOS89732.1"
                     /transl_table=11
                     /translation="MWLYHLLLIALLERLVAGTTCYFPEGNVAEDYTPCSDNGVSFCCN
                     KDSICLSNGLCTSMHQPYVLGRGACTSQSWNDTSVCDDVCHGITTAWSSACSLILYGTV
                     NGVKLYCPNSIISNGTDSLGCANGVSPISVGTGKPIAGKAYLASYSLLPTASTATSTAT
                     ATSTTEPDTSSPSTTTADESCSSSTAANSNQVSRATVTAVGAGVGVPLGLLALASLGYG
                     FNERRKRLQLQNASPNMYQMPAASSTSYRSRNVGAKELSGHIPLHELDT"
     gene            <14543..>18325
                     /gene="ASPWELWI_7468_1173616"
     mRNA            join(<14543..15423,15480..15521,15625..>18325)
                     /gene="ASPWELWI_7468_1173616"
                     /product="hypothetical protein"
     CDS             join(14543..15423,15480..15521,15625..18325)
                     /codon_start=1
                     /gene="ASPWELWI_7468_1173616"
                     /gene_functions="other (smcogs) SMCOG1272:TPR
                     repeat-containing protein (Score: 45.6; E-value: 8.4e-14)"
                     /gene_kind="other"
                     /product="hypothetical protein"
                     /protein_id="AOS89733.1"
                     /transl_table=11
                     /translation="MASGPKASHHDYTVAWICALPVELAAAQALLDEIHDQLPAGPADT
                     NVYTLGCIYGHRIVLTCLPSCVCGTISAAIVATQLLSTFHSIQFALLVGIGGGIPTGSA
                     DIRLGDVVVARPTDKHGGVVQYDFGKATPTGFQRTGILNSPPRSLLQALSKLEANHLSH
                     VGQFSSFLSELERRLPGQGALVFSRPVTEDHLYLADYHHVGTQSDGCENCDKSRTAARP
                     VRCDDLPVVHYGLIASGNQVVKDSHLRNKLGQELGAYCVEMEAAGLINHLPCLVVRGIC
                     DYADSHKNDAWHGYATPYGLFDRQYVGNFVGRGANLHELWEILRPNTAMARKAVVIYGM
                     GGLGKTQLAAHFARIHKEDFTSIFWLHGKDETTLNASFADLVARVVELAAINSTNHHAM
                     REGPGLCAKTALEWLSKKNNAEWLLIYDDVEARDIEKWLPTADHGSIIVTTRSQQIADS
                     GMIAHPLKPLPFEEALQLLTGEPGPGDSTYSRCQNDPSSEALARRLHGLPLALALAGSY
                     IHRTGMSCSKYLEYYQREWCSLQAAAEPLREYRNGNLQTAWRVSYEAVKQTSPLAAQSF
                     FVLSLFHHEDIWYELLNSAMQSHALPPWLSEAMSNEIQFSKLMQILLEFSLVQQSSRNG
                     SYCIHPVIQDWCKNELPTIDPDLFELGTKTFAIVAVAVGSNARTALDTNDWSLQHRLLY
                     HANRLTPLLRAKPGESRDAEVLSAVHTIGRLYWTHGRYERAEEMYQEALAGREMVFGLD
                     HNVTLQTVHNMGLLYHDRGDLRSAELMFQRALSGYNCTENDNAHLEALDTLQSLANLYH
                     AQGRLDEAERLCYNALTGYQSLLTANSALVMDAMHNLANIYFSQHQLPAAEELYDQALK
                     GKQRLLGEYHTSTLDTTHNLGVVYFEQGRLQEAEEMCDRALSGKVRVMGEDHTSVFDTL
                     FQLGTLYRSQGRSKAAEEMYQRALLGRQKVLGVSHSSTLHTIHHIGNLYLRQGRLQEAE
                     QMQERALNGYDSTFGHDHTYTLELAHTLAVVCCQRGKLAKAESLFQRVLAAKEQTDGKR
                     SAPVLAILNNLGNVYREQGRLVEAEKTYKLVLNEWQKLRRTHPAALGALSNLGIIYQDR
                     NQLKEAERVFKESLNGYNSELGPDHALTLDTVCNLGDLYRDQHKAHRAKELYQRALTGY
                     ENILGPDHPRTQETANKVRLLCNYSKPTERHLIARLWKGGR"
ORIGIN
        1 gtcagcaaaa cactgcggta accctgtatc accagacctg ggctatacgc ttgcttaacg
       61 agcggttggc acagccagaa ccggtcctga cctatggaac actgggagca gtgataccgt
      121 tactatacta taatgttcgt gccgtccatg tttcccccac cgttcagagt agactgttct
      181 gaaggattat tccagatggt cgcacttgac cacgactccg ctgtcgctca tcagaggggt
      241 ctagtaaaaa tgctccttgc cacgcccaaa tccttccgca acaacattgg gccactcata
      301 gccatcgtga agatgtacgt ggccattacc cccagactgt ggcttgtgac taagatgtta
      361 gtgctatgct gtcatttgca tgtatctacg acatgcaacc tatttgggac tgtctgtact
      421 ctgagtctgt tcgctcctct agcctgcttc gaaatctagt gtcaagagcg gctttgggga
      481 acggcggatc tctgttccaa aaacagacta tggatgggat tttggatgtt tacgaagccg
      541 tctcacggct cgatcagttc ttccacgccg accgcagcag tacctccagt atagaatgtg
      601 agtgggcgca tattctggca agatctagtc caacgactcc ctggaccgac cgccataact
      661 atcttacttc atctgaaaaa ctcaatgcat gctgtcacct ctcttgttcc atattctgga
      721 caaccctgcg acgacggcaa ttgcacgacg agcaagcccc aggctctacg gaccaggatc
      781 agctcaatca ggtgctccta aactatattc tgcaggtcga accactatac tggattcaga
      841 atgctcccga ggcattcacc tgggttgcat ttacaggcgc tgctgcgtcc actcaaccag
      901 gtctgcgagc caacttcatc aaccacgcgg caactgtaat cacggccatc gacggcgagg
      961 acttgactct gatacgacag ggatggaggt attttggact gatcaaaagg ctcggaggag
     1021 catgaaaggt gagcaacata tccgcatgac tgctagtata tctctcgaat agtttacgac
     1081 tattcatcga ccttcacatg tgatagcgca ttaacggtcc actcccagag ttcctccgcc
     1141 atttttgcat ctgatcccgc cttactggga gccgacagtc ttccgatcgg aagcagatac
     1201 gccccattat ggcattcgag cttcaactgc ggcgatacag ccgcgaagag aatgctggct
     1261 gctcctttgc gtggacctgg cgcacccagc tcgaccagcg gctttatcaa tctatagagc
     1321 ggggtagatg aaatcgggcc tgcagccagg cctctggtat gatgttagga atgtcagagg
     1381 tatatgaatg gaacccggga gaagagcacc tacgtcttaa cggttccggg gtgcactgag
     1441 agtgctagga tgtccggata cctcttggcc agctccctgc tgtgtaggac attggcaagc
     1501 ttcgaatggc catatcgagc ccagactgag aaggctttca tattcatatc ggagaagata
     1561 atgccttcct tcggggcgag tttggcatgt ccgtcactag acacatttac gatgcgcacg
     1621 cttcccttgt cggctgcctc ggctgctgtc tgctgcaggg ttgggagaag taaactggta
     1681 aataggaaat ggccgatgta gttgacctac atgagcttat cattagcgtc aagggttttc
     1741 tgtttgtatg cttctttccg ttttgttata gacaacggcg atgaaagtag aactgtttta
     1801 catgatggtg gcgagaagaa ggtacactca cagctagctg catctcaaaa ccatctcgtg
     1861 tttcgtcgta cggaacacac ataatctggt tgaagtgagc tgagaaatct tttacgagag
     1921 gttcagtcag gcctgggtca ccaacaccgg cattgttcac cagcaaatgt agcctcgact
     1981 cctgcctaca tcaccaggtc cggtgtatca gtcgtgagca ccgcaaacga ttgggcaatg
     2041 aaagaataag ggggattaaa gaaaatatca ataacagaga aaccgaagac acaccgaatg
     2101 aattgctcag ctgcatgacg aacgctactc atgctggaca ggtcgagatt gagaaacctc
     2161 acgtctactt cacgctccga gcattccgcg atgatgtccc gcctggcatg ctcgaaccgt
     2221 gtttgggatc gactggcaat atacacccgg gcgccatgtc ttgcgagttc gatacttgtg
     2281 ccaaaaccac tggcggagga gagagttagt aagggtacga gggaactact accagtccgt
     2341 tcgatgacaa gaacaagagg aaagccgaaa agtagcaaat gattattatt ggaaccaaca
     2401 gagtccacgt acagtcctgt gtgcccgcca gtgacgatga cgactttacc ggtcagctcg
     2461 gggatggcga tggcggaaag gaggtctttg cccatcgcga ggtctttttg gcagagtcaa
     2521 tgatagcgta aggggaacct tcagtatccc atagggggtg tagacatgta cagtaaacaa
     2581 atatatactt caaacctact gtcttcttag tatagaagct cctgctacta cacaccaggc
     2641 ggctggctgg tttgggctgg acgtgcgggc gcatctgcgg ccgcacccgc agccgtaact
     2701 gcagcatcca caacaccctt ttttttggtt cagctggatg gaatcctctc gcaggagatg
     2761 accagtgctg atgtacggaa agcttagacg catcacccta tacggttgtc gcttcgagag
     2821 tatcggtcaa ccgaaggaca acgcactact gatatgccaa gtagacaaaa cagagaaaga
     2881 agaataagaa taatcaaaga gatacaaatt taaacctaag aacaaggagc atgaagaaga
     2941 actagatagc agcctacggc ttcagcacaa cctgtccccc ccagccactc agctttcctg
     3001 ccgcatccaa tgcgcgctca atctcatcca acccaaattc ctcggtgtga gtgacgcccc
     3061 ccttctcgcc cagcggaagc aatccagacc gcaccatctg aatcagccga aacacatgcg
     3121 gacgatcata catgaaccgt ccctggatgc gaatgccctt gaacatgatc tccccgtacg
     3181 gcagctgaat attccccaaa tggccgccca tgaggacaca ccgaccgaac gaacccaggg
     3241 cgcgaatgcc ggcaactaat aaggacgagt ccttgatggt cgggggcgaa aggtccaggt
     3301 acacattggc accgtcgggg tggccgacgg ctgtctggaa ggactgggta tcgctggcct
     3361 cgtcgccagt gatttgcacg gttgtaagtc gttgcgtgcc gtggatgtgt tcgagcttgt
     3421 ccaaagctgc cctgttgcgc ccggccgcga tgacggtggc ccccatggcc agggctgcac
     3481 ccaccgcgcc accgccaaaa cgccccgtag ccggggccac gatcaccacc tcccccggtt
     3541 tcacgtccac ctcggcgagc ccgccaaaag gaaccaggta tgcggtcaga aggcagaggt
     3601 cggtgatgct gtatcccaat cgctccaaga gaacttcctc gtccagcgga tacaagttct
     3661 ccagggggaa ccgcgtgtat tcggcgtacg ttccgttgcg ccagactcca tccatgagtt
     3721 tctgtgccgc cgggtgaatt gccccgtgaa tcccatagag catggtcacg gcggggttat
     3781 cgcgcgcgcg gatggtaatg tcgcagaaga cgagctgacc cggcgtgagg gtgacacagt
     3841 cgggtccaac ttcatggatg cgcgcgatgg ccgtgttccc gggggtcatg gggatggaca
     3901 gcgggtaagg gcgttggttg gtgatgacct ccctcatgta ggggacaata tcagcagcca
     3961 ggatgcggac gatggcgtca ccagtcgatg ccacggggcg agggacattt tccacaacaa
     4021 gcggcttgtc ccgggcatga atcaccgcgg ctttatgggt tgacatcttg gtcgattcac
     4081 tcgtctggga tgattgctgg tggtccctga tgtgtagagc attctgatgc ccgtcctttg
     4141 cccttcatat aagatagaca ggtccatggt aggcgagaat cttatatccg caaactccga
     4201 ccaaccccca ccaactaagt ctggtacact actgtctact gcggagtact gagactccat
     4261 ccgtcacagt tgtgacaaaa gtggacactt ataacaacca ataaacgcca agtatatccc
     4321 tggatggtgg gttttaatca accttaacac ctctgcaact gcttgggcgg ctctggattc
     4381 atgaaaacgg cgataaactc acactccccg ccgtaactcc gagcccctct accaactagc
     4441 ctcgtatctc atgaatccga gggcattgta cgacctgcaa tgccgcacac cgcacccgca
     4501 cccgcatcga aagaggtggt cagatggttg agtggtgact ttctggcagt ccctaaacag
     4561 atatttggtg gtaattctat ggattcacac tcgcggcgca aagcacttgg tcttcttcgc
     4621 gcggtctgga gccagcggtg cggcggcata cgagttgtgt gccgatctgc gacgtgcgga
     4681 atgtgcagtg tccgatatgg tctgtgatat tactgatgca gaggctgttg agacttgaga
     4741 atgctatggc gcagtgtcaa gcgtctatgc ctcccatgcg gggttgtctc cagacttcaa
     4801 tgattctcga ggtaggtgca ttgtggggaa tgacgggtct atgctggggg cacgcaacta
     4861 acatggactg caggacttga ggctctcgac tacggaccat actcggttcc tgaaggtcat
     4921 caaaccgaag gtgcagagta ccattcacgt ggcctctgcc ctatccccca acaaatccaa
     4981 gctagattcc tttgtcatgc tctcctcatt gataagaatc attggcaacc gcggtcaggt
     5041 caactatgcg gccgccaaca ccttcctaaa tgtctttgcg gctcaactgg tcacccgggg
     5101 ctatctaccc ggccacctct atcagtctgg acagcgtgct cagggcaggg ctgggtcgcg
     5161 gagaaccagc ataaagtgcc gatggcgctt gcctacgggg cgctttcgga ggagcttttg
     5221 ctctctatcc tggaatatca tatggacacg gcctggaaag ctgcgcgaag cacccagaca
     5281 tgtcacacgg tcgttggaat tcgatcggcg caaaacttcc aacgccagtg cttccctctg
     5341 cccgggttca tggcccatcc attattcacc ccactctggg ccattgcagg cagccggaca
     5401 agtggccgag gcgccggttt cgcacgggtt gcgcgaggca acaaccatgg aggcggcggt
     5461 aaaagtcgcg acgcgagcca tcgtgcacga agtcgcgcgt attatggcac tgtcagtgca
     5521 ggaaattgat ccacagtggt cgctgggctc gtatggagta gattctcgga tgacggtgga
     5581 cttcaaggcc tggttccaac gggaagtggg gatgtccatg ggaacagcgg agctgttggg
     5641 ggagctggcc atgacccagt tggcccagca ggcggcagat gcgtcgcaat tcttgccggc
     5701 ggagctccgg ggaggttgat aaagaggccg gtatacaatt aggaaaatat atatcggaaa
     5761 tgtaattgca taatggacgc acaaaaagat aatgccagga agaatgaagc ctcgacgatg
     5821 agaaagacgg gctggcgcca tcagcaaagc attcaggcat tcaggcatca gtgctaacta
     5881 tcggaggaag atcgggccga tgatcaggtg atttgcactg aggcagagaa gtggcactag
     5941 ccccaaatag gcaacgtagc tgagaccttc tgttttggga tgaacagacg agcaaatgat
     6001 agcctgacca attgattctc aattctatcc caatatacac ctcccctgat aggaagattg
     6061 cgagatgggc aataactact actcctatag accactcgtc gatggttaag gctttaccct
     6121 cctcgccatg cttatctctg tgatcaggcc attatgggcc tccttatggg ggaacctaga
     6181 ctaggaccat ctaagcgcca taaacatctc gcttagtgga gatccaacga cgagacacaa
     6241 tcgactagcc gcaaacatgt gatgtctccg gaatggggcc cagagtccag gcatccagac
     6301 ttgattatcc ttgctggtcc ggtggtgttc ttcgattctg gctgccctac gatcatagtt
     6361 gaaatcataa cgtgtatcta agaaaaatgt gccgcaaaga aacatatggc caaaatggta
     6421 atctcgagca atccaggacc attgaccaac aggagactgc aagaaaaatc aagaccggca
     6481 cacgacgtcc gccattggcc tgtaataaaa ccgtggatcg accaccgaca caccgacgct
     6541 ccccttgaca tagcttagac caagccctgc ctcccaatag gtatagtata ctatcataat
     6601 tgtggatcac gatcattttg caacacattc ttgccgcact aacgaccgca aagtctgatt
     6661 cacgacacct catacccatg ttatgccact tgtccgtata ttcccgtctc tataagaggt
     6721 caatcttccc gagcagtaaa gcgggtcgaa tgcttggtcc tcaaacgtcg aaaacaagct
     6781 ttttgttcat gttccaattg tcctgcctag gaggacatgc cgagtacgga gatcaatcat
     6841 accagagggg ccacaccgtc cccgcggtgc cccttcgttg agtatgaacg aatcctagag
     6901 aagaacccca tcttggcccc gactggctgt acagacaaat tctgtcaagc tggacgtctc
     6961 atccacagcg atgagccaaa agtcggggag aatcgtcccc ttgaagtggt gcagaatgaa
     7021 gcagtggcat ttctctggca gctgtggcag gatggcgtgt acactgagga ctgttaccta
     7081 gaaaggcgca cacaagtcct ggaggaaatc gctcggtcgg cgaagcaaac aatcgtctgg
     7141 gagcaggagt ccaagcgagt ggacacgacc tcgatctgga cacagagccc cgaagaactt
     7201 cgctatggac tgcggctggc ctggaggaac tcgcgcaagt gcatcatgcg ctcgcggtat
     7261 caagagctgg aactgtgtga cctgcgacat attcatacca gcgtcggaat ggccacagca
     7321 ctacttgagg aagtcactaa agccttcaac catggccaga ttaagccgac aggtaggatc
     7381 aattcagtat aattgccact gtatcgcatg gtgatctgaa acgtcttctc cagtgtttgt
     7441 ctttccgccg aagtctgtgg atggcagtgg tccgatgttt tggaataaac agctgctgaa
     7501 cttcgctggt gtatgttctc cttccctctc ccccctgggg atgcctatta gtgctgatgt
     7561 gaccggactc cgcaacccgc agtacgagct cgaggatggc tctatcatgg gtgacccgag
     7621 taatgtcaag ctgacccaag acatcgttga cttgggctgg acgccgccac gggccaagac
     7681 gcgatgggat cttctaccaa ttgttgccat ggccgaaaac gacgcaccgg ctcttgtgga
     7741 ggtgccagac gacctccgtc ggttgatcga gatccaacat ccggactacc ccagtttcaa
     7801 tgaattaggc cttatgtggt accaattccc agctctgagt cgattgggtt ttgatatagg
     7861 tggagttcag tatacggcaa cgccatttat cggatggtac tagccttctc ttcatgatcg
     7921 gtttatgact atggctgacc ctgacaaggt acatggatgc ggagatcgga gtgcgtaacc
     7981 tggccgacac gttccgcttc gacgccctgc ccgaggtggt taaagccatt gggtttgaca
     8041 tcgacgccta tcgtgcaaaa tcggagtacg cggaagttca agaactggag gatctaccgg
     8101 actatgagca gctgcggtgg cagagccgcg cccaggccga gctaaattac gccgtgcgtt
     8161 ggtccttcct gcaacagggt gttacttgtg tgggaacgtt ggctgcgtcc gcggactggt
     8221 gtcagtttga cgatgaacat gccgcgaaga agggctaccg actaaactcg gatccctact
     8281 ggctgtcacc gccccaggga tcgattgtgc ctttgtggca tcggggtggt gccccgaact
     8341 accagccgaa gcctatgatc tgtcggaatc gttatgatcc cgttaactcg tggcagcgta
     8401 gaaagggctc cacggatgcg gatccagtcc ttctgataag gaaagggaat gaatgggtca
     8461 ccaaacctct gactggtttc ctcgctgata tcccaagccg aaagaggcgt aagctgaccg
     8521 agtctcaaag ggaagagaag ccctccacgg tgcatatcta ttactgcagc cagggtacca
     8581 cagcacggac tctagctgaa agactacatc ggcgagtgaa gcgcagtgcc agaggacttc
     8641 acgtgacatt tggttctttg aacgccttgg acttgactca gatcgaagca tccgatatca
     8701 tccttctagt tgcgtctacc accggcagtg gtgcctttcc tgccaatgga gctgactttg
     8761 cgaaagcgat gggacatttt tgcgagtccc tcaagaccga cttctcgcgt gtccgactgt
     8821 ctgtctttgg tgttggggat agcgcctatc ccaacttcaa tgctgcagca atcaccgcgt
     8881 acaacctttt tcaccagctg aacgtcttgc ccatagctgg tggattgatt aaggccgatg
     8941 tggcatctga gacgctgccg ttgcgcatgt tcaatcactg gtcggacgcg gtggagtccg
     9001 cattagtcgg agggaaaggc gaattgagca gcgatcccga agaatcgttt gtgtcacacg
     9061 ctgagatgct gcgcaacttt ggccgggcta cggtcatctc taaaacatgc agcggtgcag
     9121 atgatggctt aatcaccctg acacttgacg gcatcgagtg tgccgacatg gcccacctga
     9181 ggctgctccc cacgaattcc gcatctcatg tcgctcgagc catcactgcc ctgggaatct
     9241 cagatcctca gcttctcatg ccgttctcta gcggggcact ccagcagtgc agctatggca
     9301 tcttcttcac caggttcgtc gatctggagg atagaggtaa actacccagc tgggcaatcg
     9361 agtcgaacaa taacagtggg cacgagaaag aaagtatcct tgaaagactg gagcaatttt
     9421 cagtcagtct acagccatca cagttgtctc ttgagctccg tcataagatc tgctttggtt
     9481 gcccactcct tcgcccccga gccttttccg tggcatcatc ctcgcggtac atcggcgaga
     9541 agaaggtgga gctcttgatt aagccacacc ggcgaggccg attcagcgac atctacctct
     9601 ccacccttca gcctggcgga tcaatcgaat atgccatcgt ccctgcctcc cctgcatctt
     9661 ttcttcccgc tgtccaaaag cccctcgtcg tcatcagcac tggctctgga tttgcaccag
     9721 tccgcagtct acttcagcag cgcattcaga ttgcacgaga atggggagaa gagagtagca
     9781 cgccacatcc gtttcatact tctcccatca gccttttcgc agggtttcgg ctagtggatg
     9841 agagcatcat tcacgcagta gtcgacctgg cggagaggta tcgtctgttg gatctggcct
     9901 gtctcgctcc tagtaacccc gaaaagaaac gagttcaagg ctacctgctg gacaaacgcg
     9961 acacaatccg ggcgaagttg gtagaacagg aaggatatgt ctatgtttgt gggagtgccg
    10021 ctatgactga aggggtgcga gtcaacctca gcgagattct cgcgggagag gtgaaacaga
    10081 tcatgggaga acgatacgtg gaggaagtgt tttgaggggg cgagacacta acttccgctg
    10141 ggatcaacag tgctcacatc aatcaaactc ctccgtcata atgctccccc tcgtccaggc
    10201 gcccatgtca aggcgtagaa ccacctgcac tctcaggatt gtcaccatca ctatatcctc
    10261 tcttggctct gcacaaaact cctggtgcaa aggaatagta tataccgtac gtgaaatgag
    10321 tcgagacgtc ataccgtttg catgcgaggt aattttgcca agtaaccgat atccgcatag
    10381 aagcaaacgc ataccgaccg actggaaacc aaatatccca gccaaacatt ggaaagggtg
    10441 acaccatact cctcgcatgg gttttgtatt gtgctatgtt gattggtgta ggccaaccgg
    10501 gatgcgtacc gaaaaggtcc ggagatatac atatggctgc caaaggctgg gggaactcat
    10561 aagtacttat gcaatgtcca gaaaggctag acacgaacat taaatgttcc tctgtggaga
    10621 tggaggaact gaagccctcg gtccacatat gtgcagaaca cacggtgaga cccggcagag
    10681 ttaacgagaa gcatagagaa tgagaactgt tttcttccaa gtcgtgggat gcctgcatac
    10741 ttcaatgcgg gttatgtcat tacgtgacgg ggactgaagg tagctctcgg gagaaatata
    10801 taattactag tactgttacg aactactggt catttgggtg acggtttcac tagtagacat
    10861 ttgacatctc gtcctggcat gagagacaat tgtcgagcca gaaatctgtt tgccactatt
    10921 gatcgttaca tctttgaggt ccaatacgag gatatatgct tattcgatga tagtaactta
    10981 accaactcaa acaattttat cctccatgcc agtgcttaca taacgccatt tcccgggctt
    11041 ccaatcacgt gatgaccaag gccgccaagg cagcatctca cgtcagccag cctccagggg
    11101 tgatgagtga gttatacctc gtctttcact gatcgcgcct agagggacag tcgcctctct
    11161 ctttatcatc cgctagcaac cgattcctct ctcagtttat atacttctta tacatacatc
    11221 tactaatgtg acgtagacat ccatcaattc agctgggaag attctgaaca ctggagcccc
    11281 tgaaagggtc gtcgggtgtg ccctagtggc ttggcttggc ttcattttcc ctcttcgcac
    11341 ggcccccaaa acccactgac ccctcgggtg tggcagaagc agcatcagag agtgcaaggg
    11401 attggcccat tcggcagact gacattcctg gcccccccgc ccacagccaa ccggctgcct
    11461 caccgatgtc accctcgtcc ctccggcctc ttcagattga actcgcgagg ttctccctca
    11521 tgcctacctg ggatggcgtg ctcgcgtgtg tccttgatag tctgccaatc gtccgggctt
    11581 gaaaattatc tggctcttga aagggatctt ggcccccacc agcctcggca cggcccccat
    11641 tcaatcagct ttagtgtgag cctggcgcgc tgaccagcga cggtcatttc cccagatgaa
    11701 aaactccctt cgtctcgcct attctccttt cactctgttg ctgaaatgtg agatggcaca
    11761 gctattgctg ctgctgccgg cctgaaatca aatcccacct tgctttgaga gagaaaagtt
    11821 tggggggtgt tcgtcagcat gtggctttat catctgctgc tgatcgccct tctggaacgg
    11881 ctggtggccg gcaccacctg ctacttccct gagggcaacg tggccgagga ttacacgcct
    11941 tgctcggaca atggtgtctc gttctgctgc aacaaggact caatttgcct cagcaatggc
    12001 ctctgcacct ccatgcatca accgtatgtt ctcggccgcg gcgcctgtac ctcccaaagt
    12061 tggaacgata cctcggtctg cgacgatgtc tgtcacggaa gtacgtggga tacctcgctc
    12121 cccttatagt ttggcttcaa cttcttctac tgttcttctt caatgcgact gaccaaatgt
    12181 cgcaaaataa cagtcaccac cgcgtggagc agcgcctgct ccctaatcct ctacggcacc
    12241 gtgaacggag tcaagttgta ctgccccaac tcaattattt ccaacggaac cgactccctc
    12301 ggatgcgcca atggtgtctc acccatttca gtgggcactg gcaagcccat tgccggcaaa
    12361 gcctatctgg ccagctactc cctcctccct actgctagca ccgccaccag cacggcgacc
    12421 gccacttcca ccaccgaacc cgatacttcc tccccctcca ccaccacagc cgacgaaagc
    12481 tgcagcagct cgaccgcggc taatagcaac caagtcagcc gggcgaccgt gactgccgtt
    12541 ggggccgggg tcggcgtccc gcttgggctg ctggcgttgg catcgctggg ctatggcttt
    12601 aatgagagaa gaaagcgact acaactccag aatgcttcac caaacatgta ccagatgccg
    12661 gcggcctcat caacctccta ccgatcgaga aatgtgggcg cgaaggagct gtctggtcat
    12721 ataccgctcc atgagctgga cacgtgattg cgcatgagcc cgtgcatata attgttcgac
    12781 aagcgtttgg tgaaaaggga tgacagaaag ggagagtggc atacagaatc ttggtgacgt
    12841 tttgaggtca cagcaactat atgaatgatg ttgcatgtat ctccgttacc ccaacaattg
    12901 atgtcggacc gctggcttag acccgctgaa cctgtacttt tggggactca gcaagaacga
    12961 accgtaaaga taataatact aataagcttc ctccaagcag ttcatgtaca ggactaaaac
    13021 cctaatagcc ttgaatggat ccgctaaaac tccataccgt aagaaggctg cgacccggag
    13081 tgaacggggt tgagatagtg agacactttt acagccgatg tgcactgctt atcctccctc
    13141 aaaggcgtct ctgatcgtac acagaaccca tttagcagtc aagtagaata tgccgccaga
    13201 acaactctga cacaaggaga gaaacccgcg ataagtagtg ccaacagaac tagcatatga
    13261 ctcctaatgt agggtgaatc ggagaagccc tgatggaacc aaacagggat catctggcca
    13321 tgctgactgg gaaaagacgt acccgcgacc aaggaagtat attaatcttc cttcagcggc
    13381 tggagatcta cggttccagg atgttaaacc agtggtccct gattttttga agatcctacc
    13441 gcaggtccac cggaaatggt cgacgaagtc ctagccataa ccatgccgca aaaccttatc
    13501 cgacatcgga atatgtgact taaccttgct tgaacatcac acgcaaatat ctacagcagc
    13561 catgccctcc accccatgcg caagttttag gtttgatgat tcggcatact cagcgccaat
    13621 tacatgatac ttcgaaacaa gaagactcaa atgcatctgt cggagccgaa taagctatgt
    13681 gcgtggcccg acccagagat caggtttatg atgccgcccc caatccgtgg cagacatatt
    13741 tatggtgata aactaggtat ggatacacaa agaaaacctt tcgaacaaaa catgctgtcc
    13801 acgaaggaag gtattatcat ttggccggca tgcagtatcc gcatatattg tggttacaga
    13861 tggcacatct tggctggtta tcccagctct tcggaccgtc accacactgg cactatggaa
    13921 tcggtgagca ttcgtcaaat tatcaggacg atatgcatcg aagttaacct acacagaaat
    13981 ataaagtggg gaatgcatgg tacggtgcga gattgtcatg aacgcccaca acctgctcaa
    14041 tcgaccttag agccgagctg gctaagggaa ggtgtcgtat ggatggccgt ttacagagct
    14101 taaacagatc catattacct cccgatctcg aaatcaaaag acagggcgtt gcatcggact
    14161 cgtgctgctc tggtgaatgt tgatgatcta tcaccccctc gttgtctggg agaagatcga
    14221 gggagatatc atagagtatg ggagtcatgt tatagaatta tgaaagggca agatcgccta
    14281 gaactagcga cagacagacg cctagattcc tcgcatgtgg gaggtcatag actagctggc
    14341 ggggcaagga tgctgctcgt acctgtattt aatcagcggt tcttgtggct atgtcccccg
    14401 ccacagctac gattgggtag cagtcaggca ggaagataga tcggccgacg ctccagctgc
    14461 gacgcacatt atctcagcaa cacgacgtat gattttcctc gatgagaaga cacgcttagc
    14521 accacatccc cggagcttca taatggcatc cgggccgaaa gcttctcacc atgattatac
    14581 cgtggcgtgg atatgcgcac taccggtgga gctggcagct gcccaagctc ttctggatga
    14641 gattcacgat cagctaccgg ccggaccagc tgacacgaat gtctacaccc tagggtgcat
    14701 atatggccac aggattgtcc tcacctgctt accgagctgt gtatgcggga cgatctctgc
    14761 ggccattgtc gcgacgcagc ttttgtcaac tttccattcc atccaatttg ccctcctggt
    14821 cggcattggc ggaggcatac cgactgggag cgcagatatt cgcctcggcg atgttgtcgt
    14881 tgcaaggcct actgacaagc acggcggtgt tgtacaatat gactttggca aagcaacacc
    14941 cactggcttt cagcgtacgg gcatcctaaa tagcccgcct agatctctgt tacaagcgct
    15001 ctctaaatta gaagcaaatc atttatcaca tgttggtcaa ttcagttctt tcctttctga
    15061 gttggaaagg agactgcctg gtcaaggggc attggtcttc tctcggccgg tgacggaaga
    15121 ccatttgtat cttgcagatt accatcatgt tggcacccag tccgatggat gtgaaaattg
    15181 tgataagtca agaacggctg cacggcctgt ccgatgcgat gacttaccag tagttcatta
    15241 cggtttgatt gcgtcgggaa accaggtggt caaagacagc cacttgcgta acaagttagg
    15301 gcaggaacta ggggcgtact gtgtcgagat ggaagcagcc ggcttgatta accatttgcc
    15361 ctgtctggtg gtccgcggca tatgtgacta tgctgattca cacaagaatg acgcctggca
    15421 cgggtatgct gccgccacag ctgcagcgta tgcgaaggaa ctactttctg tgataccagt
    15481 tacgcaacac catatggctt attcgacagg caatacgtgg ggtaagcaac ttgaaggtct
    15541 cttgacagag catctcaccg ctaacagcta gaggccagac aactaccaca ttccatttca
    15601 gctgactgac gtacccacaa ttagcaattt tgtcggtcgt ggagcaaatc tccacgagct
    15661 ctgggagatc ttgcgcccaa acacagcgat ggcgcgcaaa gccgtagtaa tatatggaat
    15721 gggtgggcta ggcaaaacac agcttgcggc ccactttgct cgcatacaca aagaggactt
    15781 cacatcaatc ttttggctgc atggcaagga cgagactacc ttgaatgcgt cctttgcaga
    15841 ccttgttgct cgtgtcgtag aattggctgc aatcaatagt acaaatcatc atgcaatgcg
    15901 cgaagggcca ggattgtgtg caaaaacagc tttagagtgg ctctcgaaga agaacaatgc
    15961 tgagtggctt ctaatctacg acgatgtaga agcccgtgat attgaaaagt ggctgccgac
    16021 cgcagatcat ggttcaatta tcgtcacaac tcgatcacag caaatcgctg acagcggcat
    16081 gatagcccac ccactcaagc cgcttccctt tgaggaagca ttgcaactac tcactggtga
    16141 accaggccct ggggacagca catacagccg atgccaaaat gacccaagct ccgaagcttt
    16201 agctagacgt ttgcacggac tacctttagc gctggcactt gcaggctctt atatccaccg
    16261 gacaggtatg agctgctcca aatacttgga atactaccaa cgggaatggt gcagccttca
    16321 ggcagcggca gaaccgcttc gagagtaccg caacgggaat ttgcagacgg cgtggagagt
    16381 ttcttacgag gcagttaagc aaacctctcc actggcggcc cagtccttct ttgttctttc
    16441 attatttcac catgaggata tatggtatga gctactcaac agcgccatgc aatcccacgc
    16501 gttaccccca tggctctctg aagccatgtc gaacgagatt cagttctcaa agttgatgca
    16561 gatattgctg gaattctcgc tggtacaaca gtcgtcgcgc aatggaagtt attgcataca
    16621 ccccgtcata caggattggt gtaagaatga gcttccaaca atcgatccag acctctttga
    16681 gcttggcacg aaaactttcg ccattgtcgc agtcgcagtg gggtcgaacg cccgaactgc
    16741 gctagacacg aacgactggt cgctgcaaca ccgcctgctg tatcacgcaa acagactcac
    16801 gccactatta cgagccaaac caggagaaag tcgagatgcc gaggtccttt cggcagttca
    16861 tacaattgga cgcctgtatt ggacacatgg aaggtatgaa cgtgctgagg agatgtacca
    16921 agaggcactc gctgggagag agatggtctt cggcctagat cacaatgtca ctctccagac
    16981 tgtccacaac atgggcttgc tttaccacga ccggggcgac ttgagatctg ccgagctgat
    17041 gtttcaacgg gcgctttccg gatataattg caccgagaat gataatgccc acctggaggc
    17101 tctggatact cttcaaagcc ttgcgaatct ctaccatgct caagggagac tagacgaggc
    17161 tgaaaggctg tgctacaacg cactgacagg ataccaaagt ttattgacag cgaatagtgc
    17221 cttggtcatg gatgcaatgc acaacctcgc aaacatctac ttttcccaac accagttacc
    17281 cgcagcagaa gagttgtacg accaagcctt aaagggcaaa cagagactgc ttggtgaata
    17341 tcacacatcg acgcttgaca caactcataa tttaggggtt gtttattttg aacaaggtcg
    17401 acttcaggaa gctgaagaaa tgtgcgatcg tgcactttct gggaaggtga gagtgatggg
    17461 agaagaccac acgtccgtgt tcgacacgct attccaatta ggaaccctgt atcgctccca
    17521 gggtagatcg aaagcggccg aggaaatgta ccaacgagct ttgttgggac gacagaaagt
    17581 cctgggtgtc tctcactcct cgactcttca tactatccat catattggaa acctttattt
    17641 gcggcaaggc aggctgcaag aggcggagca gatgcaagaa cgagcactca acggctatga
    17701 cagtaccttc ggtcacgacc acacctatac gcttgaacta gcccataccc tggctgttgt
    17761 ctgttgccaa cgagggaagc ttgctaaagc agaatccctg ttccaacgcg tcctggccgc
    17821 taaggagcag accgatggga agcgtagtgc accagtgctt gctattctga acaacctagg
    17881 aaacgtatac agagagcaag gcagactcgt ggaagccgaa aagacatata aattggtctt
    17941 gaacgaatgg cagaaactca ggcgaaccca tcctgcggca ctcggggctc tgagcaatct
    18001 cggtatcatc tatcaagacc ggaaccagct gaaagaggcg gaaagagtgt tcaaagaatc
    18061 tctcaacgga tacaatagcg agctcggtcc tgatcatgcc ctcacactgg acacggtatg
    18121 taacctaggg gatttgtatc gggaccagca caaggcccac agggcaaagg aactctacca
    18181 gcgcgctctt accggctatg agaatattct gggtccagac cacccgagga cgcaagaaac
    18241 cgcaaacaag gtacgcttgt tatgcaatta ttcaaaaccg accgagcgac acctcattgc
    18301 ccgactttgg aaaggtggtc gttaaaacat actttgccgt gaacttcaat tatccatcag
    18361 gtttagccaa acctcatcag ataaaaggta ctgatgaact atattttgct acgaagccat
    18421 atttggttct ggtcacaatc cttctgtacc tagtcacgtc gtcctttgta tacgtatgtc
    18481 cgacaactat atcaatatct gattactctc cattacccgt taatcgggag caagtatgct
    18541 atacacggta ctgatatgcc taggacaagc atagaaaaga gcattgcaga atcggttgta
    18601 atgctctgaa aaggcaacg
//
