LOCUS       BGC0002610              6468 bp    DNA     linear   PLN 03-NOV-2015
DEFINITION  Aspergillus stellatus DNA, stellatic acid biosynthetic gene cluster,
            complete sequence, strain: NBRC 32302.
ACCESSION   BGC0002610
VERSION     BGC0002610
KEYWORDS    .
SOURCE      Aspergillus stellatus (Emericella variecolor)
  ORGANISM  Aspergillus stellatus
            Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina;
            Eurotiomycetes; Eurotiomycetidae; Eurotiales; Aspergillaceae;
            Aspergillus.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 18:05:10
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 6468
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     gene            complement(1..2640)
                     /gene="Stl-TS"
                     /gene_synonym="EvSS"
     CDS             complement(join(1..321,403..548,610..711,774..937,
                     992..1146,1197..1896,1954..2210,2270..2292,2364..2640))
                     /codon_start=1
                     /gene="Stl-TS"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1182:Polyprenyl synthetase (Score: 243.7; E-value:
                     3.6e-74)"
                     /gene_kind="biosynthetic-additional"
                     /gene_synonym="EvSS"
                     /note="terpene cyclase/geranylfarnesyl pyrophosphate
                     synthase"
                     /product="stellatatriene synthase"
                     /protein_id="BAT32889.1"
                     /transl_table=11
                     /translation="MEYKFSTVVDPGTYETHGLCEGYEVRYHKNAELEDIGCLRCQEHW
                     RQSVGPLGAFKGTLGNPFNLLSLVIPECLPDRLSIVGFANELAFIHDDVTDIVQYGDAH
                     NNDFKEAFNSMATTGSMENAASGKRALQAYIAREMVRIDKERAIPTIKAWAKFVDYGGR
                     QETTRFTSEKEYTEYRIQDIGLWFWYGLLSFAMALDVPEHEREMCHEVCRTAYVQIMLV
                     HDLASWEKEKLNAAALGKDVITNIIFVLMEEHGISEEEAKERCRETAKTLAADYLKIVE
                     EYKARDDISLDSRKYIESWLYTISGNTVWSFICPRYNSSGSFSDHQLELMKNGVPKDPA
                     SGSTNGTSNGTSNGTSHVAVNGNGHVTNDDLSANGIKTDGELLSAITMEHLKNRNSFKL
                     GDHDQEVKSLHGHGQALDPRVLQAPYEYITALPSKGLREQAIDALNVWFRVPTAKLEII
                     KSITTILHNASLMLDDVEDGSELRRGKPATHNIFGLGQTINSANYQLVRALQELQKLGD
                     ARSLLVFTEELHNLYVGQSMDLYWTSNLVCPSMHEYFQMIEHKTGGLFRLFGRLMAVHS
                     TNPVQVDLTDFTNHLGRYFQTRDDYQNLVSAEYTKQKGFCEDFEEGKFSLPMIHLMQTM
                     PDNLVLRNVWTQRRVNGTATHGQKQTILNLMKEAGTLKFTQDSLGVLYSDVEKSVAELE
                     SKFGIENFQLRLIMELLKTG"
     misc_feature    1..6468
                     /note="stellatic acid biosynthetic gene cluster"
     subregion       1..6468
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0002610"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..6468
                     /contig_edge="True"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            order(4384..4651,4741..4869,4937..5207,5303..5337,
                     5446..5474,5551..6221,6303..6468)
                     /gene="Stl-P450"
     CDS             join(4384..4651,4741..4869,4937..5207,5303..5337,
                     5446..5474,5551..6221,6303..6468)
                     /codon_start=1
                     /gene="Stl-P450"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1034:cytochrome P450 (Score: 254; E-value: 4.7e-77)"
                     /gene_kind="biosynthetic-additional"
                     /note="cytochrome P450 monooxygenase"
                     /product="stellatic acid synthase"
                     /protein_id="BAT32890.1"
                     /sec_met_domain="p450 (E-value: 3.4e-26, bitscore: 79.0,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEGSINDARRTNYLVLLRTCYEIYGIYEPLLALFAVYSVAVVVYR
                     LYLHPLARFPGPKLAAATGWYEFYHDVFRGGQYLYEIESMHRKYGPIIRINPHELVVND
                     PDFYNTVFVAANTRRTDKWSGLEGIGLRGSLAFTRDHDLHRIRRKRYEPFFSRLSVSRI
                     EPIIVDEAKLLAKQLEASSKTGRVIELEHVMSAFTGDVITTLCSEKSPDMIRHPEFGKG
                     WHTSLYNFPSCFRAGAFNSFLEYSTDHINTAKREMLSVDKLEQNNKSSVFRYVLSTDMP
                     QAERDTERLAREAALLFGAGSVTTTRFFSVTIYYTLRNRQIRDRLSAELKDVMAGYPST
                     LPTWQELDRLPYLHAIVKEGLRYACPFVLSSSLAAWSSLSHKFSSRLSYGVMRHLSRIS
                     PDSALHYKQWTIPPGTPVGMSSYSLHTDPETFPEPFKFMPERWLGEYNPKMNRSWVPFT
                     RGSRNCLGMNLAYAQIYWGLAVMFRPGGPRLELYETNESDIRPVLDFLGPLPKSGSRGL
                     RVTVS"
ORIGIN
        1 ctatccagtc ttcaacaact ccataatcag tctcagctga aagttctcta tcccaaattt
       61 gctctccagc tcggcgacgc tcttctcaac atcgctatac aagacaccaa gggagtcttg
      121 agtgaacttt agagttccag cctccttcat gaggttaagg attgtctgct tctggccgtg
      181 cgtagcagtc ccgttgaccc ggcgctgcgt ccagacattc ctgaggacaa gattgtctgg
      241 cattgtctgc atgagatgga tcatggggag agagaacttg ccctcctcaa agtcctcaca
      301 gaagcctttt tgcttggtgt actggtcatg cgtggtcaag ttagcattgg tctcgtgata
      361 cttaagcgag tagcggcaag gtcagggcag ggcagggcat acctcggcag agaccaagtt
      421 ctggtagtcg tcgcgggtct ggaaataccg acccagatgg ttggtgaagt ccgtgaggtc
      481 gacctggacg gggttcgttg agtggacagc cataagacga ccgaatagcc taaagaggcc
      541 gccggtttct atcagacgcg tccacggtga gtttcggggt tcattttcag gggcgaggac
      601 agcacgtact gtgttctatc atttggaaat actcatgcat cgaaggacag acgagattgc
      661 tggtccagta gaggtccatg gactggccga cgtagagatt gtgcaattcc tctgcaggca
      721 ggggttacaa tcagttactg atgcagcaac atccatcgtc tagtgtgacc taccggtgaa
      781 tacaagtaga ctccttgcgt ccccaagttt ctgcagctct tgcaatgcac gcaccagctg
      841 gtagttggcc gagttgatcg tctgtcccag accgaagatg ttatgtgttg ctggctttcc
      901 tcgtcgaagt tctgatccgt cttccacgtc atcaagcctg atatacggtt agcctagacg
      961 caagggaacg ggaaggaaag aaagcacaaa catcaacgag gcgttgtgga ggatcgtggt
     1021 gatcgacttg attatctcca gtttcgcggt aggtacacgg aaccagacat tcagggcatc
     1081 gatagcctgt tctcggagcc ccttggaagg aagagccgtt atatactcgt atggcgcttg
     1141 caagacctgg tgggattagc aaaggtgcta tttggcaacg tgggcgagta ttttaccctt
     1201 gggtcaagag cctgcccgtg cccatgaagg gatttcactt cctggtcgtg gtcaccaagt
     1261 ttaaaggaat tgcggttctt gaggtgctcc atggtaatcg cactgagaag ctctccatcg
     1321 gttttgattc cgttggctga aaggtcgtca tttgtgacat ggccgtttcc attgacagcg
     1381 acatggctag tgccattaga agtaccattg gaagtaccat tcgtagagcc actcgcaggg
     1441 tccttgggaa cgccattctt catcagctcg agctggtggt cggagaagga tcctgaactg
     1501 ttgtatctag ggcagatgaa actccaaacc gtgtttccac tgatggtgta cagccaggac
     1561 tcaatgtact ttctactatc cagcgagata tcgtctctcg ctttgtactc ttccacgatc
     1621 ttcaggtagt ctgccgcaag cgttttggcc gtctcacggc atcgctcctt ggcttcttct
     1681 tctgagatcc catgctcctc catcaagacg aaaatgatat tggtgatgac atctttcccc
     1741 agggccgccg cgttcagctt ctccttctcc caggaagcca aatcgtgtac cagcattatc
     1801 tgtacgtagg ctgttctgca cacttcgtgg cacatctccc gttcgtgttc ggggacgtcg
     1861 agcgccattg caaaggagag caggccatac cagaacctag agaagcaccg ggtcagtcac
     1921 tagacagcct cacgatccaa caaagcgcct caccagagcc cgatatcttg gatgcggtat
     1981 tcagtgtatt ccttctccga tgtaaaacgc gttgtctctt gccgtccgcc gtagtcgacg
     2041 aatttggccc acgccttgat tgttgggatt gcacgctcct tgtcaatgcg caccatttct
     2101 ctcgcaatgt acgcctgtag cgcccgcttt cctgaggcgg cgttttccat gctgcccgtg
     2161 gtggccatgc tgttgaaggc ctctttgaaa tcattgttgt gagcgtcacc ctgggctgca
     2221 atcagctgat tgtccttgct gaggatggcc cggggttagg gacacttaca tattggacga
     2281 tatcggtgac atctaccata cgcaaccccc ctccctcatt agcactgcca gagtgacgcc
     2341 aagagaatta gaatacaact caccatcatg tatgaaggcg agctcattgg caaaccccac
     2401 aatgctgagc ctatctggca agcactcggg gatcaccaga gacaggagat tgaatgggtt
     2461 tcccaatgtg cctttgaacg cccccaaagg acccaccgat tgtctccagt gttcctggca
     2521 ccgcaggcac ccaatatcct cgagctccgc gttcttatgg tacctcacct cataaccttc
     2581 acatagccca tgagtttcgt acgtcccggg gtcgacaacc gtggaaaatt tgtactccat
     2641 actgggaggc tatgaacgcg accaggcaat agaagaagat gaggaagagg tgcacgagga
     2701 gagagtatgc aagggatggc tgagatgagc tcgatgacat ctgcggcata ccacaatcat
     2761 ggccaaagga aacttgagaa aggtgttgca tttaagactt ccggacctgc ctagcaacaa
     2821 caaaacgaaa ggacgtcagc taatagcctc cttgacggtc aggcagatcc ccagtgtcag
     2881 ggaacccaat catatcatgt ccaacaaccg atagctctgc caaatccgct cagcaattct
     2941 caatcgaccc ggtgtcgaat cgcgtccaat aaccaccgca gtgcgtgata gctcgtgctg
     3001 ggggagccgg accagtatag catcgcatcc aatagtcatc cagctcatga attccgaact
     3061 gggtcagcca tcaaatcacc tattgcgggg tataatcgct cggaatggct cggaatcatc
     3121 aacttggtcc agtgtaatat ggcgtccaat ggccatgcca gtaccagatt gcgcgcgata
     3181 tccgccaggc caggacagaa tctgcttcca ggaagccagc gagcatttag acatgcagtg
     3241 gcagatcgct catgattata aactggaccg gtggaggatt catacatagt attacgaagg
     3301 cggcggcgtc tccaaagtgg gtggaattag ccgacgcagc ctcaacattg gtcacaaccc
     3361 caaactgatc atgaatctat atcagcttga tagcggactg aggcgttctt ggcacccccg
     3421 ggagggcagg ggcaggggca gatcgccact gcttgctgtc cgtctgaata ttcggaatac
     3481 gcggaacagc ccaggccctg tagatcgcgc agcacagaca gggcagaccc tgcaaggaga
     3541 cgatgcatat tgacctgggc tgacatgcga tactctgaga ggcctgcaaa cagatgggac
     3601 cggttgagaa aaatgcaatt gctcttgacg ccctgcgtca tcattggtac acgacaggtc
     3661 tcgcagagaa accgtggagg tgattcgggc tgacgtgcag tactctgaaa agcctgcaaa
     3721 ccggtgggac cggccgagaa gtgacttatc gaaagaaaaa ataagcattg ctcttacacc
     3781 ctgtcttatt gtcgtacacg gcaggtctcg tgagggaagc tatggtgatt caggctaatg
     3841 tgcagtactc tgagaggtgc cgccaaacag gcatgcactg ggtcggaaag tgactgatcc
     3901 agcaaggact gatcaaaggc tgatggtgag ctgatggtga gctgatggat ccatcatcct
     3961 cattcatcct gcaacccggt cgtacggggc caggtgttga gggaaaagag agctggactc
     4021 agaaaaagga agccgagaat ccaggactgg gatgatgcat tgagcagatc cttatggtct
     4081 ttgcaagggg gcgacctacc caacagcctg gctggccgcg tgtgacggca gacagaggca
     4141 ttgaggacat ggcagtcgag ctggcgatga aggggcctcc ccattcccca gcaaggaaca
     4201 ccctgcctgt caccgggaga acgtgggcct ctccttgctt catggaggag ctccgcgctt
     4261 gcagtgcgga ttaaattgtc tgttggtcct cataccccat tcaatcctcc ccagctggac
     4321 catttgccca acccatttga acagtgtctc ccacggccat tgtacaaggc accgcctcta
     4381 gtcatggagg gctcaatcaa tgacgctagg cgaaccaact accttgtcct cctgcgcact
     4441 tgttatgaga tatatgggat atacgaaccg ctcttggcgc tctttgctgt ctatagcgtt
     4501 gcagttgtcg tctatcgcct ctaccttcac ccgctagctc gtttcccagg cccgaagctt
     4561 gctgctgcta ccggatggta tgagttctat catgatgtct ttcggggagg gcagtatctg
     4621 tatgagattg agtctatgca caggaaatat ggtacaggga ccccctcccg agggtgaggg
     4681 tatatctctt gcttcattct accattgcta acaagacaaa ccaaattgaa aaccatccag
     4741 gccctattat ccgcatcaac ccgcatgagc tcgtagtgaa tgacccagac ttttacaaca
     4801 ccgtcttcgt cgccgccaac acccgccgta cagacaaatg gagcggcctt gagggtattg
     4861 gacttcgcgg tacggcgtct cctggcctct tctaacctct tctagagaat ggtatggcta
     4921 atactctagc caccaggctc gttggccttc acccgggacc atgatctaca tcgtatccgc
     4981 cgaaaacgct atgagccctt cttctcgcgt cttagtgtga gccggattga gcccatcatt
     5041 gtcgatgaag caaagctgct tgcaaagcag ctcgaagcat ctagcaagac cggccgggtc
     5101 atcgagctcg agcatgtcat gtctgcgttc acgggggatg tcattaccac tctttgctcg
     5161 gagaaatccc ccgacatgat aagacacccg gagtttggca agggctggtt agttgcaata
     5221 tcctctcctt tcgtgatagc tacctaccag aatcctcacg ctagcctgca ggcatcagac
     5281 attccatact gtgctacggc aggcacacat ctttgtacaa cttccccagc tgctttagtg
     5341 agtgcactca tttcccgggg ggggggggga ctgaccatgc ctaataactt tacaatagca
     5401 ttgctcgact gtttccaaca gctctgctaa agcttttgct cccaggggct ggagccttca
     5461 atagcttcct cgaggtacgt actgaccaaa gcgggactcc caccccccgg agccacacac
     5521 tgacccccgg tcctcgtcaa cggcaatcag tactcaaccg accacatcaa caccgccaag
     5581 cgcgagatgc tcagcgtaga caagctcgag caaaacaaca agagctccgt gttccgatac
     5641 gtcttatcaa ccgatatgcc ccaggctgaa cgtgacactg agcggctcgc tagagaggcg
     5701 gccctgctct tcggcgcagg cagcgtgact accactcgct tctttagcgt gaccatctac
     5761 tataccctca gaaaccgtca gatcagagac cgtctctctg ctgaactgaa agacgtcatg
     5821 gccggttatc cctccactct gcctacttgg caggagctgg acagactgcc gtacctgcat
     5881 gctatagtca aggaagggct ccggtatgct tgcccttttg ttctctctag ctctctagct
     5941 gcctggtcgt ctctctcaca caagttctct tctagactca gttacggggt aatgcgccat
     6001 ctctcacgca tctcgcccga ctccgcgctc cactacaagc aatggactat cccccccggt
     6061 acccctgtgg gcatgtcgtc atacagcttg cacacagatc cagagacctt ccctgaaccc
     6121 ttcaagttca tgcctgagcg ctggctaggc gaatataacc ccaagatgaa taggagctgg
     6181 gtccccttca cgcggggctc aaggaactgt ctaggcatga agtaagaaag tcccccctcc
     6241 ccaaccttct tgtccatacc ttgatcaaaa tgggtgcaac tgagcggcta acaatttacc
     6301 agtcttgcat acgcccagat ttactggggg ctagctgtga tgtttaggcc cggtgggcct
     6361 agactcgagt tgtacgagac aaatgagtcc gacattcgcc ctgtcctgga ttttctggga
     6421 ccccttccga aatcgggtag tagggggctg agggttacgg tgtcctag
//
