LOCUS       BGC0002633             11574 bp    DNA     linear   BCT 17-JUL-2013
DEFINITION  Leptolyngbya sp. PCC 7376, complete genome.
ACCESSION   BGC0002633
VERSION     BGC0002633
KEYWORDS    .
SOURCE      Leptolyngbya sp. PCC 7376
  ORGANISM  Leptolyngbya sp. PCC 7376
            Bacteria; Cyanobacteriota; Cyanophyceae; Pseudanabaenales;
            Leptolyngbyaceae; Leptolyngbya.
COMMENT     URL -- http://www.jgi.doe.gov
            JGI Project ID: 4087633
            Source DNA and Organism available from Cheryl A. Kerfeld
            (CKerfeld@lbl.gov)
            Contacts: Cheryl A. Kerfeld (CKerfeld@lbl.gov)
            Tanja Woyke (microbe@cuba.jgi-psf.org)
            Whole genome sequencing and draft assembly at JGI-PGF
            Annotation by JGI-ORNL
            The JGI and collaborators endorse the principles for the
            distribution and use of large scale sequencing data adopted by the
            larger genome sequencing community and urge users of this data to
            follow them. It is our intention to publish the work of this
            project in a timely fashion and we welcome collaborative
            interaction on the project and analysis.
            (http://www.genome.gov/page.cfm?pageID=10506376).
            ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 18:07:18
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 11574
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             1..1401
                     /EC_number="2.6.1.76"
                     /NRPS_PKS="Domain: Aminotran_3 (54-397). E-value: 7.5e-97.
                     Score: 316.2. Matches aSDomain:
                     nrpspksdomains_Lepto7376_4147_Aminotran_3.1"
                     /codon_start=1
                     /db_xref="InterPro:IPR004637"
                     /db_xref="InterPro:IPR005814"
                     /db_xref="InterPro:IPR015421"
                     /db_xref="InterPro:IPR015422"
                     /db_xref="InterPro:IPR015424"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013:aminotransferase class-III (Score: 434.3;
                     E-value: 7.6e-132)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="Lepto7376_4147"
                     /note="PFAM: Aminotransferase class-III; TIGRFAM:
                     2,4-diaminobutyrate 4-transaminases; COGs: COG0160
                     4-aminobutyrate aminotransferase and related
                     aminotransferase; InterPro IPR004637:IPR005814; KEGG:
                     syp:SYNPCC7002_G0025 diaminobutyrate--2-oxoglutarate
                     aminotransferase; PFAM: Aminotransferase class-III; PRIAM:
                     Diaminobutyrate--2-oxoglutarate transaminase; SPTR:
                     2,4-diaminobutyrate 4-transaminase; TIGRFAM:
                     Diaminobutyrate-2-oxoglutarate transaminase"
                     /product="diaminobutyrate aminotransferase apoenzyme"
                     /protein_id="AFY40272.1"
                     /sec_met_domain="Aminotran_3 (E-value: 4.7e-98, bitscore:
                     317.5, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQQLYQNNREIRAAKPADSGQWRSLPGPRSQEYLKRQEARESNAR
                     SYPRRITLAIKEAKGIFIKDVDGHTYFDCLSGAGTLALGHNHPVVLEAMQQTLDGSYPL
                     HTLDLTTPIKDQFVEQLFASLPPKFAKVAKIQFCGPTGADAVEAALKLVKTATGRRSIL
                     SFSGGYHGMTNGSLSLTGSLAPKQEISGLMPDVHFLPYPYNYRCPFGLGGDDSQKVSAK
                     YIEHLLDDPESGIVKPAGMILEAVQGEGGVIPAPDHWLREMARITSERGIPLILDEIQA
                     GIGRTGKLYAFEHAGITPDVVLLSKAIGGSLPLSVVLYNKNLDLWKPGAHAGTFRGNQL
                     AMAAGAATLKFIIENNLAHQAQVLGDRLLSHLREIQKDHPCLGDVRGRGLMLGAEIINP
                     RMGITAHPELAKQIQAECLQRGLILELGGRFGTVVRFLPPLIITAEQIDAVSEIFAESV
                     AAAEQKLA"
     protocluster    1..11574
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[2961:10453]"
                     /cutoff="20000"
                     /detection_rule="IucA_IucC"
                     /neighbourhood="14000"
                     /product="NI-siderophore"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      2962..10453
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="IucA_IucC"
                     /neighbourhood="14000"
                     /product="NI-siderophore"
                     /protocluster_number="1"
     cand_cluster    1..11574
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="IucA_IucC"
                     /kind="single"
                     /product="NI-siderophore"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..11574
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0002633"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..11574
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NI-siderophore"
                     /region_number="1"
                     /rules="IucA_IucC"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            1..1401
                     /locus_tag="Lepto7376_4147"
                     /note="IMG reference gene:2503889781"
     aSDomain        163..1191
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_Lepto7376_4147_Aminotran_3.1"
                     /evalue="7.50E-97"
                     /label="Lepto7376_4147_Aminotran_3.1"
                     /locus_tag="Lepto7376_4147"
                     /protein_end="397"
                     /protein_start="54"
                     /score="316.2"
                     /tool="antismash"
                     /translation="IKEAKGIFIKDVDGHTYFDCLSGAGTLALGHNHPVVLEAMQQTLD
                     GSYPLHTLDLTTPIKDQFVEQLFASLPPKFAKVAKIQFCGPTGADAVEAALKLVKTATG
                     RRSILSFSGGYHGMTNGSLSLTGSLAPKQEISGLMPDVHFLPYPYNYRCPFGLGGDDSQ
                     KVSAKYIEHLLDDPESGIVKPAGMILEAVQGEGGVIPAPDHWLREMARITSERGIPLIL
                     DEIQAGIGRTGKLYAFEHAGITPDVVLLSKAIGGSLPLSVVLYNKNLDLWKPGAHAGTF
                     RGNQLAMAAGAATLKFIIENNLAHQAQVLGDRLLSHLREIQKDHPCLGDVRGRGLMLGA
                     EII"
     gene            1457..2965
                     /locus_tag="Lepto7376_4148"
                     /note="IMG reference gene:2503889782"
     CDS             1457..2965
                     /EC_number="4.1.1.86"
                     /NRPS_PKS="Domain: Beta_elim_lyase (194-334). E-value:
                     5.1e-08. Score: 24.3. Matches aSDomain:
                     nrpspksdomains_Lepto7376_4148_Beta_elim_lyase.1"
                     /codon_start=1
                     /db_xref="InterPro:IPR002129"
                     /db_xref="InterPro:IPR015421"
                     /db_xref="InterPro:IPR015422"
                     /db_xref="InterPro:IPR015424"
                     /db_xref="InterPro:IPR021115"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1180:Decarboxylase, pyridoxal-dependent (Score: 386.3;
                     E-value: 2.9e-117)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="Lepto7376_4148"
                     /note="PFAM: Pyridoxal-dependent decarboxylase conserved
                     domain; COGs: COG0076 Glutamate decarboxylase and related
                     PLP-dependent protein; InterPro IPR002129; KEGG:
                     syp:SYNPCC7002_G0024 L-2,4-diaminobutyrate decarboxylase;
                     PFAM: Pyridoxal phosphate-dependent decarboxylase; PRIAM:
                     Diaminobutyrate decarboxylase; SPTR: L-2,4-diaminobutyrate
                     decarboxylase"
                     /product="L-2,4-diaminobutyrate decarboxylase"
                     /protein_id="AFY40273.1"
                     /transl_table=11
                     /translation="MSLLQVAHRFERYFLSAHPVSLEHYRDAIAISQETLIRNLLQKDK
                     SYSGATPNELIEELASPYYFNFEAQEWSQIQGQLAHIVSNSVVVHDPTCIAHLHCPPLI
                     PAIAAELIIGATNQSMDSWDQSPCATVLEQKLTDWLCSTFGYGCNADGTFTSGGTQSNL
                     MGLLLARDNYAKVHLNWQVQKEGLPPEATRFRILCSEVAHFTVRQGAALLGLGENAVVT
                     VPADDNFCMRAADLEARLIELQNQNLLPIAIVGTAGTTDFGSIDPLTEIAQIAQKHDIW
                     FHVDAAYGGALQLSNKHSTKLSGIEQADSITVDFHKLFYQPISCGAFLLKNRANFGLIR
                     LNADYLNPEINEEQGIPDLVTKSIQTTRRFDALKLWLSLQVLGVEAFDEMVNTTIDLAQ
                     SAAQLIDGDRHFELANKNPAINAVVFRYFGDRPEAVDLEQINQQIPKQLMLMKKAVIAQ
                     TQVNKKTHLKFTLLNPLTQLEHLQSLLAEIKVLGQKLERDYPKQ"
     aSDomain        2039..2458
                     /aSDomain="Beta_elim_lyase"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_Lepto7376_4148_Beta_elim_lyase.1
                     "
                     /evalue="5.10E-08"
                     /label="Lepto7376_4148_Beta_elim_lyase.1"
                     /locus_tag="Lepto7376_4148"
                     /protein_end="334"
                     /protein_start="194"
                     /score="24.3"
                     /tool="antismash"
                     /translation="RILCSEVAHFTVRQGAALLGLGENAVVTVPADDNFCMRAADLEAR
                     LIELQNQNLLPIAIVGTAGTTDFGSIDPLTEIAQIAQKHDIWFHVDAAYGGALQLSNKH
                     STKLSGIEQADSITVDFHKLFYQPISCGAFLLKNRA"
     gene            2962..4806
                     /locus_tag="Lepto7376_4149"
                     /note="IMG reference gene:2503889783"
     CDS             2962..4806
                     /codon_start=1
                     /db_xref="InterPro:IPR007310"
                     /db_xref="InterPro:IPR022770"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NI-siderophore: IucA_IucC"
                     /gene_kind="biosynthetic"
                     /locus_tag="Lepto7376_4149"
                     /note="PFAM: Ferric iron reductase FhuF-like transporter;
                     IucA / IucC family; COGs: COG4264 Siderophore synthetase
                     component; InterPro IPR007310; KEGG: syp:SYNPCC7002_G0023
                     siderophore biosynthesis protein; PFAM: IucA/IucC; SPTR:
                     Siderophore biosynthesis protein"
                     /product="IucA/IucC family protein"
                     /protein_id="AFY40274.1"
                     /sec_met_domain="IucA_IucC (E-value: 1.3e-69, bitscore:
                     223.5, seeds: 168, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNSRQIAEAATIQSFLNCYLRETGNFKSLAADDPLTLQLKERTNA
                     KSAIICPLAQQNIEILVGVKYWSLSDRHIFTFPLFYKCAARDQLLELDYLTLSTLLLKE
                     LSLSMGSTEPYDELIERVIQSCNNIQQFVEARQPKASELYEFKRNFGETEQSLVLGHHL
                     HPTPKSRQGFLSQEMTDYSPETEGEFSLHYFRAHKSIIIEGSTLEESATELIKTELRND
                     DAVSEAFKATYCGNDDYALVPVHPWQGRWLLHSEKVQNLIGKELLEDLGQQGRKFKPTS
                     SVRTVYNADARFMVKLSLNIKITNSVRANLFKELERGLEVNQIFSSTIGKDLRFHFPNF
                     EVIRDPAYITIPLDGKESGFATILRANPFQTKAKQIDNLNTDSESDASCLIGLCQDALD
                     GSGSRLANIIRTLATQENRSTEKVSIDWFKQYLSLSVKPMLWMYFTYGIALEAHQQNSV
                     LELKDGYPKCFHYRDNQGYYYRESFSDKLNGILEGISQKSETFCPDEVIDECLVYYVFI
                     NNVFGIINALGVAELVDENLLLRELNILLAEFADFNLAGSKLLDNLQSETFRTKANLLT
                     RFNNMDELVGSVATQSVYVNIQNPLHTVKTMAKEEAYA"
     gene            4799..5398
                     /locus_tag="Lepto7376_4150"
                     /note="IMG reference gene:2503889784"
     CDS             4799..5398
                     /codon_start=1
                     /db_xref="InterPro:IPR016181"
                     /db_xref="InterPro:IPR019432"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1203:putative siderophore biosynthesis protein (Score:
                     192.4; E-value: 2.5e-58)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="Lepto7376_4150"
                     /note="PFAM: Siderophore biosynthesis protein domain; COGs:
                     COG1670 Acetyltransferase including N-acetylase of
                     ribosomal protein; KEGG: syp:SYNPCC7002_G0022 IucB family
                     siderophore biosynthesis protein; SPTR: Siderophore
                     biosynthesis protein, IucB family; acetyl transferase"
                     /product="IucB family siderophore biosynthesis protein"
                     /protein_id="AFY40275.1"
                     /transl_table=11
                     /translation="MPDLETQIQPVYEVYDRTIPGKISFRPISLELDLERIHSWMNKPH
                     VIPFWQMNFPIEKIKSYLIKVLADQHQTAYIGLLDDEPMSYWESYWVVDDILGKFYTAE
                     SADQGIHLLIGEPYFLGKGLALPFLRAITMFQFQHTSTTKTVTEPDARNAKMIHIFKKC
                     GFEFQKNIDLPDKTGSLMFCDRQRFESLWSPAELYQ"
     gene            5449..6756
                     /locus_tag="Lepto7376_4151"
                     /note="IMG reference gene:2503889785"
     CDS             5449..6756
                     /EC_number="1.14.13.59"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1080:lysine/ornithine N-monooxygenase (Score: 426.6;
                     E-value: 1.5e-129)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="Lepto7376_4151"
                     /note="COGs: COG3486 Lysine/ornithine N-monooxygenase;
                     KEGG: syp:SYNPCC7002_G0021 siderophore biosynthesis
                     protein; N-monooxygenase; PRIAM: L-lysine 6-monooxygenase
                     (NADPH); SPTR: Siderophore biosynthesis protein;
                     N-monooxygenase"
                     /product="L-lysine 6-monooxygenase (NADPH)"
                     /protein_id="AFY40276.1"
                     /transl_table=11
                     /translation="MNNQVYDLLGVGIGPFNLSLAALLEPIADVNAVFLEQKSQFNWHS
                     GLLLEAATIQVPFLADLVTMADPRSRFTFLNYLREHSRLYHFYFLEEFKIYRREYNHYC
                     QWVSEQLGETCQFGQNVEAIAWNDSGYFTVMARDHGTGETVTYNAKHLALGVGSKAGIP
                     ECLRKLGKQDNIFHSGQFLHHKEQWQNAKSVTVIGSGQSAGETFYELLNAQADCGYHLE
                     WHTRSAGFFPMEYSKLGLEYFSPDYIEYFYDLSIEKRDEIRSNQNLLYKGMDEGLIAEI
                     YNRFYELTIGGTELDVKLCSSLEVKEAEITESGYRLSYRQVQQDHYFEHDTDCVVLATG
                     YDHVVPEFIEPIRDLIKWDYKDRYCVNLDYRVTLTKSIPNSIFVQNGELHTHGIGAPDL
                     GLGAHRSSVIINTFLEREVYPTAKKNVFQEFGVKSI"
     gene            6753..7970
                     /locus_tag="Lepto7376_4152"
                     /note="IMG reference gene:2503889786"
     CDS             6753..7970
                     /codon_start=1
                     /db_xref="InterPro:IPR011701"
                     /db_xref="InterPro:IPR016196"
                     /db_xref="InterPro:IPR020846"
                     /gene_functions="transport (smcogs) SMCOG1184:major
                     facilitator transporter (Score: 57.6; E-value: 1.5e-17)"
                     /gene_kind="transport"
                     /locus_tag="Lepto7376_4152"
                     /note="PFAM: Major Facilitator Superfamily; InterPro
                     IPR011701; KEGG: syp:SYNPCC7002_G0020 major facilitator
                     transport protein; PFAM: Major facilitator superfamily
                     MFS-1; SPTR: Major facilitator transport protein"
                     /product="major facilitator superfamily MFS_1"
                     /protein_id="AFY40277.1"
                     /transl_table=11
                     /translation="MNNSLNRSISVLFVCTFTYIFSEVLLAPFYPVFFEKVFGVEDLTY
                     TGFYIFICRMTVVVTAPFWGFFSNKLNIKTLLYCGQICSALVCVFMTFTQNVTQFTIAT
                     VILLLFRSSFVILYPLIIQLGGEQRRETIAGTYQAVFHSGIVISSIAGIWIIQLENPLN
                     AFFVVALLDILQFALCWFFLEDLPKKEVIANDSKSETKTTDILQSNWALIPAIGVIGLL
                     FQFIHNLVRPYFVSYVTEADYFGTTLTTGGILFAIPSVMAIAALPFIRSYAKPHRIPML
                     YTIGSLLLAVTLLVQGSTNSLSLFVFSRVIYGFFLAVTQAALELYLFNNSDPEKIPLNY
                     TLMFAFANVGLVLSPLTASHLVEEYSFASPLIVAAVLSVFTLAYTWSLALNPVLAKTVT
                     RYQRSA"
     gene            8033..10453
                     /locus_tag="Lepto7376_4153"
                     /note="IMG reference gene:2503889787"
     CDS             8033..10453
                     /codon_start=1
                     /db_xref="InterPro:IPR007310"
                     /db_xref="InterPro:IPR016181"
                     /db_xref="InterPro:IPR019432"
                     /db_xref="InterPro:IPR022770"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NI-siderophore: IucA_IucC"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1203:putative siderophore biosynthesis protein (Score:
                     101.1; E-value: 1.5e-30)"
                     /gene_kind="biosynthetic"
                     /locus_tag="Lepto7376_4153"
                     /note="PFAM: Ferric iron reductase FhuF-like transporter;
                     Siderophore biosynthesis protein domain; IucA / IucC
                     family; COGs: COG4264 Siderophore synthetase component;
                     InterPro IPR007310; KEGG: syp:SYNPCC7002_G0019 IucA/IucC
                     family siderophore biosynthesis protein; PFAM: IucA/IucC;
                     SPTR: Siderophore biosynthesis protein, IucA / IucC family"
                     /product="IucA/IucC family protein"
                     /protein_id="AFY40278.1"
                     /sec_met_domain="IucA_IucC (E-value: 7.1e-73, bitscore:
                     234.2, seeds: 168, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSNIQALNAALNPEIWEAVSLKLLTKMLSEFMYEEIIQPDVIDDK
                     EDIVTYQLNLPDGLGYRFTAKLRIFDSYRVDPKSIYRGENDEWTPATNPFQFALDIHET
                     VGMTGETTAHLLKELTNTLIADAHIHQRKHSKEVDLLNLSYAELEGEMEGHPWITFNKG
                     RIGFGYDDYLQHAPESKQPIQMTWLAIAREQSSYNGITDLDYERLIGEELSAETIARFN
                     VLLQERGLEQEKYYFMPVHDWQWKNIVVPIFGEAVALQSIVYLGKSEDLYLPQQSIRTF
                     VNTTHSKKRHVKLPLSILNTLVYRGLPNERTQIAPKVTEYIKAIHANDSFLSKECRMIL
                     PGEVASLNYNHDYYKQLSSAPYQYKEMLGCLWRESVLSYTDPDEKPITLASLVHLDADG
                     YPFILKLVDASGLSLEAWLDSLFNTVLPPLLHYLYQYGTVFSPHGENTILVLKENIPHR
                     LAMKDFVDDVNISNQPLPELLNLSEDLKKVLLTEPPEGLCQFIFAGLFVCHHRYLSDLL
                     DQHICYPEVKFWRQVRQAILNYQAKFPQLQDRFELFNLLAPKFTKLCLNRNRLITYGYN
                     DDSDRPHAVAYGKVRNALYTTLKDEAYELWQTRKAQFSKATEQPFTVTDKQYNREISFR
                     RLTYEDLGIMHQWMQEKHLSLFWKLNVSLAEFKWYLKKCLTNESRDMVMAYSGDEPMGF
                     GIIYQMASDPLRHYYDYDKSDLGAHIAVGRREFLEPEFLIPGLRACAQFVFETYETKRL
                     VGESDIKNAILIPALESIGYENRGMIRLHHKRASLTTLDRDRFYKYLEKSQKK"
     gene            10570..11574
                     /locus_tag="Lepto7376_4154"
                     /note="IMG reference gene:2503889788"
     CDS             10570..11574
                     /codon_start=1
                     /db_xref="InterPro:IPR013094"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S9"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1066:alpha/beta hydrolase domain-containing protein
                     (Score: 241.4; E-value: 2e-73)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="Lepto7376_4154"
                     /note="PFAM: alpha/beta hydrolase fold; COGs: COG0657
                     Esterase/lipase; KEGG: syp:SYNPCC7002_G0018
                     esterase/lipase; SPTR: Esterase/lipase"
                     /product="esterase/lipase"
                     /protein_id="AFY40279.1"
                     /sec_met_domain="Peptidase_S9 (E-value: 3.7e-08, bitscore:
                     22.1, seeds: 64, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAIDPEALAISQKIKQRMMELKHPPLSELDNLDAVEARYFFHEGN
                     KLYDLHLEAGVDFEDRQIERLNSPPLNIRIYHTTKAQQNSDQDTVTSLPILVYLQGGGW
                     VFGTLDSADDTCSFLAKYTECIVISVDYRHAPEHKYPLPIIDAIDATKWVYKNAVNLGG
                     DHNRIVLGGESAGGNLAAVASIKLRDEGKIPLAGQLLITPVTQYGFETPSYKSGHRCGL
                     SPEAMHWFWHNYLESSTQVEKWEVSPLKVRDASNLPPTVMVVAEHDPLCDEGLMYAERL
                     KVSGNSVKILQYPSLIHGFIRLTHKVKTAKVAFHEIAENLKSLCYQTEFSKSI"
ORIGIN
        1 atgcaacagc tttatcaaaa taatcgagag attagagcag caaagcctgc tgactctggt
       61 caatggcgat cgctacctgg accgagatct caagagtatt taaagcgtca agaagctaga
      121 gagtctaatg ctcgtagcta tcctcgccga atcacgttag caatcaaaga agcgaaaggg
      181 atttttatca aagatgttga tggccacacc tattttgact gtttgtctgg tgctggaacc
      241 cttgccctag gacacaatca tccggttgta ctcgaagcga tgcaacagac tttggatggt
      301 agttatccac tccatactct ggatttgacg acacccatca aagatcaatt tgttgagcaa
      361 ctgtttgcaa gtttgccgcc gaaatttgcc aaggttgcaa aaattcagtt ctgtggcccc
      421 actggggcag atgcagtaga agcagcactc aaattggtca aaacagcaac cggacgtcgg
      481 agcatcctct ctttttctgg cggttaccat ggtatgacca atgggtcttt gagtttgact
      541 ggaagtttag cgccgaagca agaaatatca ggattaatgc cggatgtaca ttttttgcct
      601 tatccctaca attaccgttg tccattcgga cttggtggtg atgatagcca aaaagttagt
      661 gcaaagtaca ttgagcatct tctggatgat cctgaaagtg gcattgtcaa accagcgggg
      721 atgattcttg aggcggttca gggagaaggt ggggttattc ctgctcctga tcactggttg
      781 agagaaatgg ctcgaattac cagtgagcga ggtattccat taattctgga tgagattcaa
      841 gctggtatag gacggactgg aaaactctat gcatttgaac atgcgggaat taccccggat
      901 gtcgtgctac tgtcaaaggc cattggtggt tctctgcctt tgtcagtggt gttatacaac
      961 aagaatctcg atttatggaa accgggagct catgctggca cgttccgggg taatcagtta
     1021 gcaatggccg ctggcgccgc cacgctgaaa ttcattatag agaataactt ggctcatcaa
     1081 gctcaagttc taggcgatcg cctattgagc catttgcggg aaattcagaa ggatcatcct
     1141 tgtctgggcg atgtccgtgg tcgtggtttg atgcttggcg cagaaattat taatcccaga
     1201 atgggcatca ctgctcatcc cgaactcgcc aaacagattc aggcagaatg tctacaacgc
     1261 ggtctgattc ttgagttggg cggcagattt ggcactgtgg ttcgcttctt gccaccactc
     1321 attatcacag cagaacagat tgatgctgtt agtgagattt ttgcggaatc agttgcggcg
     1381 gcagagcaga agcttgctta agttgtcgct atcatgcccg ctcttcttct gatctatttt
     1441 ttagccttca ttacttatgt cacttctcca agtggcgcat cgctttgaac ggtatttttt
     1501 gtcggctcat cctgtgagtc ttgagcatta tcgtgacgcg atcgccatct cccaagaaac
     1561 ccttattaga aatttattgc aaaaagataa atcttattca ggagctactc ctaatgaatt
     1621 aattgaagaa ttggcgagtc cgtactactt caatttcgag gcgcaggaat ggtctcagat
     1681 acaagggcaa ttggcacata tcgtgtctaa ttctgttgtt gttcacgatc ccacttgtat
     1741 cgctcattta cattgcccac cattgatccc ggcgatcgcc gccgaactta tcattggtgc
     1801 aaccaatcaa tctatggact cctgggatca aagtccctgt gccacagtcc ttgagcaaaa
     1861 gttaacggac tggctctgtt ctacttttgg gtatggctgt aatgctgatg gcacatttac
     1921 aagtggtgga acccaatcaa atttgatggg tttgctcttg gcaagagata actatgcaaa
     1981 ggttcacctg aattggcagg ttcagaaaga agggttgccc ccagaagcga ctcggttccg
     2041 gattttatgt tcagaagtgg cgcattttac ggtgcgtcaa ggagctgctc tcttgggttt
     2101 gggtgaaaac gccgttgtca cagtgcctgc cgatgacaac ttttgtatga gggctgctga
     2161 tctagaggct cgattaatag agcttcagaa tcaaaatctg ttgccgattg ctattgtcgg
     2221 gacagctgga accacggatt ttggcagtat cgatcctctt acagagatcg ctcaaattgc
     2281 tcagaaacat gacatttggt ttcatgttga cgcggcatac ggcggtgctt tgcaactcag
     2341 taataagcac tccactaagt tgtctgggat tgagcaggcg gattcaatca ctgttgattt
     2401 ccataagctc ttttaccaac ctattagctg tggtgctttt ttgctgaaga atcgggcaaa
     2461 tttcggcttg attcgtctta atgctgatta cctcaaccct gaaattaatg aagagcaagg
     2521 tattccagac cttgttacga agtcgattca aacgactaga cggtttgatg cgttgaaatt
     2581 atggctatct ctgcaagttc ttggtgtaga ggcttttgat gagatggtca ataccaccat
     2641 cgatctcgcc cagtctgccg cacaactgat tgatggcgat cgccacttcg agctagccaa
     2701 taaaaatcca gccattaatg cagtggtgtt tcgttatttc ggcgatcgcc cagaggcagt
     2761 ggacttagaa caaattaatc agcaaattcc gaagcaactg atgctgatga aaaaagctgt
     2821 gattgcccaa acccaagtca ataaaaaaac ccatttaaaa tttaccctcc tcaatcccct
     2881 cactcagctt gagcatctcc agtccctact ggcagaaata aaagtactag gccaaaaact
     2941 agagcgagat tacccaaaac aatgaatagt agacaaattg ccgaagcagc aacgattcag
     3001 agctttttga attgttattt acgtgaaact ggaaatttca aatccctcgc agcggatgat
     3061 ccccttaccc tccaactaaa agagcgtacc aacgcaaagt cagcgattat ttgccctctc
     3121 gctcagcaaa atatcgagat tcttgttggt gtgaagtact ggtctctcag tgatcgccac
     3181 atatttacat ttccgctctt ctataaatgt gcagctcgcg accaacttct cgaactagac
     3241 tacttaaccc tgagtaccct actcctcaaa gaattgagtc tcagcatggg atcaacagag
     3301 ccctacgatg agttaatcga aagagtgatc caaagctgca acaacattca gcaatttgtc
     3361 gaagcaagac aacccaaggc gtcggaacta tacgaattta aacgcaattt tggcgagaca
     3421 gaacaatccc tcgtcctcgg ccatcatttg catcccacac caaaaagtag acaaggcttt
     3481 ttatctcagg agatgacaga ttactctcca gaaacagaag gagaattttc actccattat
     3541 ttccgcgccc ataaatcaat catcatcgaa ggttctaccc tcgaagaaag tgccactgag
     3601 cttatcaaaa cagaattgcg caatgacgac gctgtttcag aagcctttaa agctacttat
     3661 tgcggtaatg atgactacgc attagtgcca gtacatccat ggcaaggccg ttggttacta
     3721 cattccgaaa aagttcaaaa cctaattggc aaagaactcc tcgaagattt aggccaacaa
     3781 ggacgcaaat ttaaaccgac ttcctctgta cgaacggtat ataacgcaga tgctcggttt
     3841 atggtgaagc tttcactaaa tattaaaatc actaattcag ttcgagccaa tctctttaaa
     3901 gaattagaac ggggtttgga ggtgaatcaa atcttttcca gtactatcgg caaggattta
     3961 cgtttccatt tcccaaactt tgaagtaatt cgtgatccgg catatatcac cattcctctt
     4021 gatggcaaag agtcaggttt cgccacaatt ttgcgagcta atcctttcca aactaaagct
     4081 aagcaaattg acaacctaaa taccgattca gaaagtgatg ccagttgttt aatcggcctt
     4141 tgccaagatg ctctggatgg cagtggttct cgtctggcta atattattcg taccctagct
     4201 acacaagaaa accgttctac agaaaaggtc agtattgatt ggtttaaaca gtatttatct
     4261 ctatcagtta aaccaatgtt atggatgtac ttcacttacg gtattgccct agaagcgcac
     4321 caacaaaata gtgttcttga actcaaagat ggatatccga aatgttttca ttatcgcgat
     4381 aatcaaggct attactacag agagtctttt tctgacaagt taaacggtat tctcgaagga
     4441 attagccaga agagtgaaac attttgtcct gatgaagtca ttgatgaatg ccttgtttac
     4501 tacgtcttta tcaataatgt ctttgggatt attaatgccc ttggcgtagc tgagctagtt
     4561 gatgaaaatc tcctattaag agaactaaat atattacttg ctgaatttgc cgattttaat
     4621 ttggcaggct ccaaactcct tgataattta cagagtgaaa ccttccgtac taaagctaat
     4681 cttttaactc gctttaacaa tatggatgag ttggtcggat ctgttgcaac gcaatcagtt
     4741 tacgtcaata ttcaaaatcc tttgcataca gtcaaaacaa tggctaagga ggaagcttat
     4801 gcctgattta gagactcaaa ttcagccagt ttatgaggtc tatgatcgaa caattccggg
     4861 aaaaatatct tttcgaccaa tatctctaga attagattta gagcgcattc atagctggat
     4921 gaataaacct catgtcattc cattttggca gatgaatttt ccaattgaaa agataaaatc
     4981 ttatctcatt aaagtattag ccgaccaaca tcaaactgct tatattggct tattagatga
     5041 tgaaccaatg agctattggg aatcatactg ggtagttgat gatattcttg ggaaattcta
     5101 tacagcagaa agtgctgacc aaggcattca tttattaatc ggagagcctt attttcttgg
     5161 aaaaggtttg gctttacctt tcttacgagc aatcacaatg tttcagtttc agcatacttc
     5221 gaccacgaaa acagtaacgg aacctgatgc tcgtaatgcc aagatgattc acatttttaa
     5281 aaagtgtggt tttgagtttc aaaagaatat tgatcttcca gataaaacag gatctctaat
     5341 gttctgtgat cgccagagat ttgaaagctt atggtctccg gctgaactct atcaataaat
     5401 aatttctacc taatgtattc gttctaatga aaatcagtca gtgtagcaat gaataaccaa
     5461 gtttacgatc tactcggggt tggtatcggc ccatttaatt taagcttagc ggctttgctg
     5521 gagccaattg ctgacgttaa tgcggtgttt ctcgagcaga agtcccaatt taattggcat
     5581 tctggcttac ttctagaagc tgcaactatt caagtaccat ttttggcgga tctagtcacc
     5641 atggcagatc ctcggagtcg cttcactttt ctgaattatc tccgtgaaca ttctcgtctt
     5701 tatcattttt attttctaga ggaatttaag atttatcgcc gggaatataa ccactactgt
     5761 cagtgggttt ctgagcaatt aggcgagacc tgtcagttcg gtcaaaatgt tgaggcgatc
     5821 gcctggaatg attctggtta cttcactgtt atggctcgcg accatggcac tggtgaaaca
     5881 gttacctaca acgcgaaaca tttagccttg ggggtcggta gtaaagctgg tatccccgaa
     5941 tgtctgcgga aactgggcaa acaggacaat attttccatt caggacaatt tctccaccac
     6001 aaagagcaat ggcaaaacgc taaatctgtg acggtgattg gttctggtca gagcgcaggg
     6061 gaaacattct acgaactcct caatgcccaa gcagactgtg gctaccatct cgaatggcat
     6121 actcgctccg ctggtttctt cccgatggaa tattcaaaat tgggtctgga atatttttcc
     6181 cccgattaca tcgaatattt ctatgatttg tccattgaaa agcgtgatga aattcgctcc
     6241 aaccaaaacc ttttgtataa aggcatggac gaaggattaa tcgctgaaat ttacaatcgg
     6301 ttctacgaat taaccatcgg cggtactgaa ttagacgtga agttatgctc ctctctagaa
     6361 gtaaaagaag ccgaaatcac ggagagtggc tatcgtctca gttaccgtca agtacagcag
     6421 gatcactact ttgaacacga cacagattgc gttgttctag ccactggtta tgaccatgtg
     6481 gttccagaat ttattgaacc gattcgcgat ttgattaagt gggattacaa agatcgttat
     6541 tgcgttaact tggactatcg tgtcaccctc acaaaatcta ttcccaactc aatttttgta
     6601 cagaatggag agcttcatac ccatggtatc ggtgcaccag atttaggatt gggagcacat
     6661 cgcagctcag tcattattaa tacgttcctt gaacgtgagg tctatccaac agcaaagaaa
     6721 aacgtattcc aagaatttgg tgttaaatcc atatgaataa ttctttgaat cgttctattt
     6781 cagtcttgtt cgtctgtacc tttacttaca ttttttcaga ggtattactt gctccttttt
     6841 atccagtttt ctttgagaaa gtatttggtg tagaggattt aacctacaca ggattttata
     6901 tttttatctg ccgcatgact gtggtggtga cggcaccatt ttggggtttt ttctccaaca
     6961 aattaaatat caaaactcta ctctactgtg gtcaaatctg ttctgcccta gtctgcgttt
     7021 ttatgacttt cactcagaac gtaacgcaat ttacaattgc cactgttatt ttgttgctat
     7081 ttcggagtag ttttgtgatt ctttatcccc tgattattca attgggtggt gagcaaagac
     7141 gggagactat tgccggaacc taccaagctg ttttccatag tggcattgtt atttctagta
     7201 ttgctggcat ctggattatt cagctcgaaa atcctctcaa tgcctttttt gttgttgctt
     7261 tactagatat attacagttt gctttatgtt ggttcttttt agaggatcta cccaagaaag
     7321 aagtcatagc gaatgatagt aaatctgaga caaaaacgac agatattctc cagagtaact
     7381 gggcattaat tccagcaatt ggagttattg gtttactgtt tcagttcatt cataatcttg
     7441 tgcgtcccta ctttgttagc tatgtaactg aagcagatta ttttgggaca actttaacaa
     7501 caggaggtat tctttttgca attcccagcg taatggcgat cgccgcatta cctttcatac
     7561 gtagttacgc gaaacctcac cgtatcccta tgttgtacac catcgggtca ttactactag
     7621 cggtgacctt acttgttcaa ggctcaacga attctttatc actttttgtt ttttcgagag
     7681 tgatttatgg cttcttcctt gctgtgactc aggcagcttt agaactctat ttattcaaca
     7741 atagcgatcc agaaaaaatc cctcttaatt acactttaat gttcgccttt gccaatgtgg
     7801 gattagttct atcaccttta acagcttctc atcttgtcga ggaatacagt tttgcatctc
     7861 ctctaatagt ggcagctgtt ttgtcggtct tcacattagc atatacctgg tcgctagcat
     7921 taaacccagt gcttgcaaaa actgtgactc gatatcaacg tagcgcttaa aaaacgttta
     7981 ttttttcttt tattaattac cctatttatc tagtgattta accctgttaa ctatgagtaa
     8041 tattcaagcc ttaaatgccg ctttaaaccc tgaaatttgg gaagctgtta gtctcaaact
     8101 tctgacaaag atgctttccg aatttatgta tgaggagatt atccaaccag atgttattga
     8161 tgacaaagaa gatattgtca cttatcagct gaatctgcct gatgggctgg gatatcgctt
     8221 tacagccaag ctacgtatat ttgatagcta tcgtgttgac ccaaagtcga tttatcgtgg
     8281 agaaaatgat gaatggacac cagcaacaaa tccttttcag tttgcactgg atattcatga
     8341 aactgttggg atgacaggtg aaacaacagc tcatttgttg aaggaactca caaatacctt
     8401 gattgctgat gctcacattc atcaacgcaa acatagtaaa gaggttgatc tcctcaatct
     8461 gagttatgcc gagctagaag gcgaaatgga aggtcaccct tggattactt tcaacaaagg
     8521 tcgcattggg tttggttatg atgattatct tcagcatgca cccgaaagta agcagcctat
     8581 ccaaatgact tggttggcga tcgcccgtga acagtctagt tataacggca tcacagatct
     8641 cgattatgag cgtttaatcg gagaagaatt atccgctgaa actatcgcaa gatttaatgt
     8701 ccttttgcaa gagcgcggcc tagaacaaga aaagtattac ttcatgcctg tgcatgattg
     8761 gcagtggaag aatatcgttg tgccgatttt cggcgaagcc gttgcattac agtcgattgt
     8821 gtaccttggc aagagcgaag atttatacct gccacaacag tccatccgca cattcgtaaa
     8881 caccacccat agcaaaaaac gtcacgtaaa attgcccctc agtattctta atactttggt
     8941 ttatcgtggc ttaccaaacg agcgtactca aatcgcacca aaggtcaccg agtacatcaa
     9001 agccattcat gccaatgatt cattcctcag caaagaatgt cggatgattc ttcctggtga
     9061 agtagctagt ctcaactaca atcacgatta ctacaagcag ctctccagcg cgccttatca
     9121 gtacaaagaa atgctgggtt gtctctggcg ggaaagcgtt ctcagttaca cagatccaga
     9181 cgaaaaaccc attactttag cttccctagt tcatttggat gccgatggtt atcccttcat
     9241 tctcaaactg gtagatgcct ctggtctctc cttggaagct tggttagata gccttttcaa
     9301 cacggtttta ccgccactat tgcactacct ctaccagtac ggtactgtct tctctcctca
     9361 cggtgaaaat acaattctcg ttctcaaaga gaatatccct catcgcctcg ccatgaagga
     9421 ttttgttgac gatgtaaata ttagtaacca gccgttacca gaactactta acctctctga
     9481 agatctcaaa aaagtgttac tgacagaacc gccagaaggt ctatgtcaat tcatttttgc
     9541 aggtttattt gtttgtcatc atcgctatct ctcagatttg cttgatcaac atatttgcta
     9601 tcccgaggta aagttctggc ggcaggtacg acaagccatc ctcaactatc aggcgaaatt
     9661 cccacaactc caagaccgct ttgagttgtt taatctgctt gctccgaaat tcactaagct
     9721 ctgcttaaat cgaaatcgct tgatcaccta tggctacaac gatgacagcg atcgccctca
     9781 tgcggtagct tatggaaaag ttagaaatgc tttgtatacc actttaaaag acgaagcata
     9841 tgaactttgg caaactagaa aagctcagtt ttcaaaagca acagaacagc catttactgt
     9901 cactgacaaa caatataatc gtgagatctc ttttcgtcga ctgacctatg aagacttagg
     9961 cattatgcat caatggatgc aagaaaaaca tctcagttta ttttggaaac tcaatgtttc
    10021 tttagctgaa tttaaatggt atctcaagaa atgccttact aatgaatcca gagatatggt
    10081 gatggcttat tctggcgatg aaccgatggg ctttggcatt atttaccaga tggcaagcga
    10141 cccattacgt cattactatg actacgataa atctgacctt ggtgcacata ttgccgttgg
    10201 tcgtagagaa tttcttgaac cagagttttt gattcctggc ttgcgagcct gtgcccagtt
    10261 tgttttcgag acctatgaga cgaagagact tgttggtgaa tcagatatta aaaatgcgat
    10321 tttaattcct gctctagaat ccattgggta tgaaaatcgt ggcatgattc gacttcatca
    10381 caaacgtgcc tctctcacaa ctttagatcg agatcgcttc tataagtact tagaaaaatc
    10441 tcaaaaaaag taaggagaaa tagtcaaaat taatcgcaat agccaattat cttaaaggct
    10501 ttaagatgat tagctaaaaa tatcatttca cctaaagaaa aaatcatttc aaattcaaaa
    10561 tcagtaatta tggcaattga tcctgaagct ttagcaattt cacagaaaat caaacagaga
    10621 atgatggaat taaaacatcc tcctctcagt gaactagaca atttggacgc tgttgaagca
    10681 cgttattttt tccatgaggg aaataaactt tacgatctgc atttagaagc tggcgttgat
    10741 tttgaagatc gacagataga acgcctgaat tctcctcctc tcaacatccg tatttatcac
    10801 acaactaaag ctcaacaaaa ttcagatcaa gatacagtaa cttccttacc aatcctcgtc
    10861 tacttacaag gaggaggttg ggtattcggg actctagata gtgccgatga tacatgttcc
    10921 ttccttgcaa aatatacaga atgtattgtt atttctgtcg attatcgtca tgccccagaa
    10981 cataaatatc ccctccccat tattgacgct attgatgcga cgaaatgggt ctataaaaat
    11041 gcagttaatc tgggggggga tcataatcgt atcgttttgg gcggcgaaag tgcaggtggt
    11101 aatttggctg ctgtcgcctc catcaaactg cgagatgaag gaaaaattcc gttggctggt
    11161 caactcctca ttacacctgt tacgcaatac ggctttgaaa caccatccta taaatcaggc
    11221 caccgttgtg gactatcacc ggaagcaatg cattggtttt ggcataacta tttagagagt
    11281 tccactcaag tagaaaaatg ggaggtgtct ccattgaagg tcagagatgc gagcaatctc
    11341 cctcccacag tgatggttgt agccgaacat gatccgttat gtgatgaggg tttaatgtat
    11401 gcagagcgtt taaaagtctc tggaaactct gtaaaaatac tacagtatcc ttctcttatt
    11461 cacggattta ttcgcctcac tcataaagtg aaaacagcaa aagttgcgtt ccatgaaata
    11521 gctgagaatc tcaaaagctt gtgctatcaa actgagtttt ccaaaagtat ttaa
//
