LOCUS       BGC0002644              7903 bp    DNA     linear   PLN 26-JAN-2022
DEFINITION  Chondria armata rad1 domoic acid biosynthetic gene cluster, complete
            sequence.
ACCESSION   BGC0002644
VERSION     BGC0002644
KEYWORDS    .
SOURCE      Chondria armata
  ORGANISM  Chondria armata
            Eukaryota; Rhodophyta; Florideophyceae; Rhodymeniophycidae;
            Ceramiales; Rhodomelaceae; Chondrieae; Chondria.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 18:08:29
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     gene            complement(1..1317)
                     /gene="radA1"
     CDS             complement(1..1317)
                     /codon_start=1
                     /gene="radA1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1052:Terpene synthase/cyclase metal-binding domain
                     protein (Score: 58.5; E-value: 1.2e-17)"
                     /gene_kind="biosynthetic-additional"
                     /product="RadA1"
                     /protein_id="UJI64970.1"
                     /transl_table=11
                     /translation="MKVLAEDDPNDALARIKTSGLTLTEPNDLYWMVKYLKEEFYQKGK
                     YYYPMKTICDGQLIETEFYCPFEPNLSTHYSKLFNTRDKRSELYAVAPNKTNMKKYNRI
                     NLEKIGALMTPTSTYQETELTGLMYSMMYYLNDQTAHLKLSKEEIQPELVDALNDHVLL
                     YLGLFLDIFEPRDPQDLERIWDFLEFYQPYFKKCKGKIVLQENYRGHTPPQIALVKKIT
                     GYISKNFAPTKNITQYIFEIVRYVKGIKEEIKIRSDKNFTLTLEEYDAFRDTVTTSPIA
                     HTITDMTFENFCYSLYLNPLFTELEDRTSEIITYFNDVCTCDRERRDNDPFNAVFILRE
                     DKSLNYPQSCDLVVMHAFNKMKRFLELKKILIEEAVTEEEKEAVKQMIHTRENTLIGYV
                     CHEICCVTNGYARDHKPLMKEYLEKKISAELVKIISTAT"
     source          1..7903
                     /db_xref="taxon:860625"
                     /isolate="SIO"
                     /isolation_source="Kyushu Island, Japan"
                     /mol_type="genomic DNA"
                     /organism="Chondria armata"
     misc_feature    1..7903
                     /note="rad1 domoic acid biosynthetic gene cluster"
     subregion       1..7903
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0002644"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..7903
                     /contig_edge="True"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            complement(4647..5699)
                     /gene="radC1"
     CDS             complement(4647..5699)
                     /codon_start=1
                     /gene="radC1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) 2OG-FeII_Oxy"
                     /gene_kind="biosynthetic-additional"
                     /product="RadC1"
                     /protein_id="UJI64971.1"
                     /sec_met_domain="2OG-FeII_Oxy (E-value: 2.9e-13, bitscore:
                     38.0, seeds: 135, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFTIKGTELNLDFNPLEVEKLNWYPRSSLPPQIPAIDLNKINTKE
                     ELGQFLDDIRKSGLFYIINHGIPEDVSMGCYNSFRKFCNLPEETRMKYNTDESFQNGGY
                     MPFKGTSVGGGNKFTKNKDFVVKYFWRGPAVLNRSPDPDFQRNHDEHHKRTAELAEKIT
                     NTILKALRTRFPNFDPEELNHNINPRNMFFSNRIYPEIPPEDGENAKFRLVPHRDLSFI
                     TLANQLPAQNGFKGLFIVTGDNEKVYVPPIRNSYLIFIGQGLSYLTNKFLPAALHGVEF
                     PGKENFEGSERSSLISFYEPDDKMTPSMNINPSQDEIFEESCKFYDDVGADKNGTTFTY
                     VKYKFLHGYY"
     gene            6320..7903
                     /gene="radD1"
     CDS             6320..7903
                     /codon_start=1
                     /gene="radD1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1034:cytochrome P450 (Score: 202.2; E-value: 2.5e-61)"
                     /gene_kind="biosynthetic-additional"
                     /product="RadD1"
                     /protein_id="UJI64972.1"
                     /sec_met_domain="p450 (E-value: 4.4e-51, bitscore: 161.6,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNTESNFLFSGVIFFSILFIVLTLVFVSLVLIFIVLRNAIQLYHL
                     RHIPTPANDQYSLVTRLFRQDPKYTAAWLNTQAISHGKSVIRLSGLLLSTVVIPITPSS
                     IQILSTNSMSFPKSPDFIRRLRDFTGTDSIFTVGTAKEHRFLRNIISPPLRYDYVVSKL
                     HVIFFREVRSVLHSFPSEQENDDDVVGVIRTATFRIVMQACFGLDTVEHSKIDMLGHLY
                     HKALSQPSYISIANLLFLPSWFPTYFFSSSDASSLQLRREVSKICDDILKNLHPDSNPI
                     FSNVTQDSLVAHMIDFLRSEPSSKSVTLTSEKLVGTVLSFLFAGQATTTLAIAWALHLL
                     SISDDVQKDLHTELQSIGDSLEDLDRIPLLDGIVKETLRLYPPVQYISRTVHNPKKGGD
                     IIDGLFIPNGTTLKIPTLALQTRSDLWGDDANEFLPKRWMKGDGKQEGENYKWMKWAFM
                     AFWHGSYGCIGQKFALLEMKVFIKEIVSKYEVKPALVVSDDGKCKVNSSPKRYGLSEPY
                     GLKLFFSPRI"
ORIGIN
        1 tcaagtcgct gtactaatta ttttaacgag ctcggcagag attttcttct ccaagtactc
       61 tttcattaac ggtttatggt cacgtgcata tccatttgta acacagcata tttcgtgaca
      121 gacatatcca attaaagtat tttctcgagt atggatcatt tgcttgactg cttctttttc
      181 ctcttctgtc acagcttctt caattagaat tttctttagc tccaggaatc tcttcatttt
      241 attgaaggca tgcattacaa caagatcaca agattgaggg tagttgaggg acttatcttc
      301 tcttagaatg aacacagcgt tgaatggatc attgtcacgc ctttcacgat cacaagtgca
      361 aacatcattg aagtacgtta tgatctctga agttcgatcc tcaagctcag taaagagtgg
      421 gttgagatat aaactgtagc aaaagttttc aaacgtcata tctgtaatcg tgtgagcaat
      481 tggacttgta gtaaccgtat cacgaaatgc atcgtattct tcgagagtca aagtaaagtt
      541 tttatcactt cgaattttga tttcttcttt gatacccttg acatatctga ctatttcgaa
      601 gatatactga gtgatgtttt ttgtaggagc gaagttttta gagatgtaac ctgttatttt
      661 cttgaccaaa gcgatttgcg gtggtgtatg acctcgataa ttttcctgta acacaatttt
      721 acctttacac tttttaaaat atggctggta gaattccaag aagtcccata tgcgctccaa
      781 gtcttgagga tctcgaggct caaaaatatc caggaacaaa ccaagataaa gcaatacatg
      841 atcgtttagt gcatctacaa gctccggttg aatctcctcc ttagaaagct tcagatgagc
      901 tgtctgatcg ttgagatagt acatcatcga gtacataaga ccagtcaact cagtctcttg
      961 gtaagttgag gtaggggtca ttagggcacc gatcttctcg agatttatac gattatactt
     1021 cttcatgttt gtcttgttcg gcgcaacagc atatagctca gatctcttat cacgtgtgtt
     1081 gaacaattta gagtagtgcg tactcaaatt tggctcgaac ggacaataga actctgtttc
     1141 gataagttgc ccgtcacata ttgttttcat aggatagtaa tatttacctt tctgataaaa
     1201 ctcctccttc aaatacttca ccatccagta aaggtcgttc ggctctgtca atgtcagccc
     1261 agaggttttg atacgggcta gtgcatcatt tgggtcgtct tctgcaagta ccttcatgtt
     1321 aactctcgtt ttgattggaa tgtgattttc tgtgaagttc tatggaaagc tggttatgtt
     1381 gttgcagttg taacaagagc ctgagaggtt agcttaaata ctttttggta gaatgactag
     1441 ggggtctgaa ttggtagatg tttcatgacc attagctgat gatggctgtt ttcgtaaagg
     1501 aaataaaaat ttaattctat tttgcaaagt acaaaacgtt tgctctctta aaaataattt
     1561 aaatatttga ggaacaacgt catttcatat caagatttga attaaaattt taatcgtttt
     1621 tgaaaggaac tataactgtt actaacctat tgctcttgtc atcattctcc ggtgtaagta
     1681 gcctcttaca gaaagtttga acaatttacc atttaaattc aaaaaagcac ttctatacac
     1741 ggctattaga cagtaaaaag cttcagaagt tttaatggaa aggcacaaca aacgagttgt
     1801 gataaaattc gtcagcaaag aagaaagtaa taaagtagat tgatagttat atttttcagt
     1861 tattttaaaa tttaaaactt ctattatttc ggaaaagaca aagaaatttt actctttgga
     1921 cataaattca tgaaaaagga aaggaaacat ggaggggata gatgtttcat tcattacttt
     1981 catctttcat caagaagagc cattcttttg ataaggaatg tctcaaattt tcaatcaagc
     2041 ttgagtgtct gaacagaagg actagtgaga taacatcgcg cttctatttt attcttcaaa
     2101 tcaattattc ctcaaagaga ccaggaaaaa gtcataaact gtatgtttac gatggacatc
     2161 aattttactg aaaaatcagc tgtctcgacc aagaaaaatg taataaaatg gaatataaaa
     2221 aatagttatt tgctactgag ataatgctga ttggtataaa taataataca tgtcgaaaaa
     2281 acatttagat ggataagtat tttaccttgc ctccattgca atcgaaaagt ttaaggtgag
     2341 tcaatattcc ttttgagtgg aggtcctgtt catttcaaaa ttacaataat cactgaaaaa
     2401 atcaagagat tcttcttttt aacacatatt tatcagaaac ctaagttaaa aatattttct
     2461 taaacatttc tcaacttaga aaaatgcact aggaactgtc acaatttgtc cttctcgaca
     2521 aaaagggaaa atcatgaaaa gaaaggatac ctctcctcaa ccaaaaatta cctgaccaag
     2581 attttcttca ttgatttatg atgcctttcg gtttagaaat atctgaatgc ctgaaagtag
     2641 tttagtgatc acttgttagt cctttatatg gtaacttctt ggtgcttcga caattttatt
     2701 tttttttaaa ttgcacaaca cttttgaatg aagtacctaa accaatttta aaaaaaacac
     2761 ccttaaagaa agtacaaatc ttgtaatggt attatcttac tgtacaatgg ttagtaatat
     2821 aagtatttat tgtcttcata caagtctttt ggtcatacta cagtatgtct aaatgaaagt
     2881 ttgcagactc atttaatgag gcaaaaataa ttctatgaaa ttcttaaaac atttcattta
     2941 tgcaagcgtt gaacaaatgt ttaccccgct ctttaaaaac gaggaaaaat ttagggtttt
     3001 caaattttcc agacaaagat ttaaaatctt gaatcactgg tatcaataag tagttacaaa
     3061 catgaatagc ggacagatag actcaacgcg aatggaggga atatacgtat ccacatatat
     3121 aaactgtagt tcttacctag aatggatgac agtaaacctt attcaccgag aatacaagac
     3181 agatggacgc aacttgaata gggggaattt cgatatacac acatattaaa cttctgttct
     3241 tacattgaat tgatggtttt aaaatgcata aatgaggcac gtcaaaacta taagttcaaa
     3301 gatgctgtcg gtcaaaatga gtcctgcttc aacatttaag aactttatta atggaagcag
     3361 tgatctatga acactttgat cttttcatgt gaacgaacag aattgtccaa tagatctttg
     3421 aatgttcaat cctatgatca tttaggttat agtacaatca gcaaaattga gaacacaaat
     3481 ctcttctttt ttcgcacgaa ggataactca atctttgaaa tttcaaacat tgagtttcag
     3541 tgatcttgca tacctcacgt ttgagctttt cgactgtctt ttctctgtgt acgagaaagc
     3601 ttcatctttt caggtcgtaa aagcgtgtta gctgaaatag atgtgtccca tctttgagat
     3661 gttctacaag atatggcatg gaatgctgca atagaaaaac gataaactag ggatattact
     3721 tttactgtca ggtgatctaa gttaacgtga tatgatgaag atatgtttta tgaagtcatg
     3781 agatgaatca aaatgtattt ggctgctcta cgtcaaacaa atgattaaca tgtcattgac
     3841 ctacctttta ataaatcaca gatatggacg gtttatattt gtcgttaacc caagagcatg
     3901 ttcttgattg agatgacatg tcacgtttga ttgataattc gactaatacg ccctgtgata
     3961 tactatattg actatcataa agagtgaata tatatatata tctttatata tctatacata
     4021 aatgactata catcgattat tatacatatt cgatatcctt ccttgaagaa aaattgtagt
     4081 cagataaact tatagcattg aggttcattc tcttgtagat aatttataaa atatcctctt
     4141 attacaacga atttcatatc tgaataaaga aacatcatat tgggcttcca gtggagggat
     4201 atgagtagaa ctaatgtttg atgacatgtc atttcatgtg aaaaccgttt ccagtaaagg
     4261 atatttccga tgaaaggact gcctcagcta gtaaagaaat gctccaaaat acgtggattt
     4321 ttacttagaa aaagtatttg cctctgcgaa aacacgactt accaccctgc aggtttcaaa
     4381 aaatctattt gcattgactt gcaatataaa aatagataat aaagcattaa ttcttaccac
     4441 aaaaatcaga aatcaagcca tgatgtttat catgacctaa attttaattt taacaacgtc
     4501 atgagtcact agactctcta cctgagtatg tggaggctag aaaaataatt ttagtaggat
     4561 agacaacttt agaatagtat attttgacgt tttagagtac ttttctaaca aattataagg
     4621 tgtagataaa tgggaagggt ctactgctag tagtagccat gaagaaactt gtattttacg
     4681 taagtaaatg tcgtaccatt cttgtccgct cctacatcat cgtagaactt acagctctct
     4741 tcaaaaatct catcttgtga agggttgatg ttcatagatg gcgtcatttt gtcgtctggc
     4801 tcgtaaaagc tgatcaatga ggacctttca cttccttcaa agttttcctt ccccggaaat
     4861 tcgacaccat gtagtgcagc aggtagaaat ttgttggtga gataggataa accttgacca
     4921 atgaagatca agtaactgtt tcgtataggt ggaacataaa ctttttcatt atcccctgtc
     4981 acgataaata gtcctttaaa accattttgt gcaggtagct ggtttgccaa tgtgatgaaa
     5041 ctaagatctc tgtgtggaac tagacgaaac ttggcatttt ctccatcttc cggaggaatt
     5101 tctggataga ttcgattact gaagaacata ttacgaggat tgatattatg attcaattct
     5161 tcaggatcaa aatttggaaa acgagttctc aaagccttca atatcgtatt tgttatcttc
     5221 tcagccagtt ctgctgttcg cttatgatgt tcgtcatggt ttctttgaaa atccggatcg
     5281 ggagacctat tcagaacagc agggcctctc cagaaatatt tcacaacaaa atctttattt
     5341 tttgtaaact tgtttccacc acccactgaa gttcctttga aaggcatgta cccaccgttt
     5401 tggaagcttt catcggtgtt gtacttcatt cttgtttcct cagggagatt acagaatttt
     5461 cggaaggaat tatagcatcc catggacaca tcttcgggga ttccatgatt aataatgtag
     5521 aaaagtccag actttcgaat atcatcaaga aattgaccta gctcctcttt tgtattaatc
     5581 ttatttaagt cgatggccgg aatttgtggg ggtagagatg aacgtggata ccaattgagc
     5641 ttctctactt ccaagggatt aaaatctaag ttcagttccg ttcctttgat cgtaaacatt
     5701 tccaggtttt acgtccaaac gttagggaaa ctgcagcctt tatcgttcca aaattgtttg
     5761 aaataagtcg agccaatctg aatagtacag taaaaagagt gtctgaatga atcgaataga
     5821 ttaattttgc tatgatactg atcgaatgtt gaagttaata agtaaacaat agattcaaca
     5881 aattaagaaa gaacagaatc agatatttaa tttatgaaat attactgatc ttacgagatt
     5941 tttaaagcct gactgaaatg gaataagtgt caaacatgtt tcaactatga tgttcacttc
     6001 atgaagaaaa ctgaaacata aaattctttg tatgaatatt aatgtagttt atgtatttgc
     6061 cgaacatcat taaattattg aaatttgtgg tgagtatgcg tactcttaaa acattgaaat
     6121 tgaaatgctt ttcaaaatga aaatattcga agaattttta aaagacttag catatactct
     6181 tttccgaatt cagttttacg tatgttcttt ttctttcatc ttgtcgtctg ctcttctaat
     6241 ctatcgttct ttacgacgga ctctaccttc atctgatttt gtctcttaga acgttttgga
     6301 gtctcatttc ccaagagaaa tgaataccga atctaatttt cttttttccg gtgtgatttt
     6361 tttctccatt ctttttattg ttctgacatt ggtattcgta agcctcgtat tgatatttat
     6421 tgtgcttcga aatgcgatac agttgtatca tcttcgacat atccctactc ctgctaatga
     6481 tcaatatagt cttgtcacac ggcttttccg tcaagatcct aagtatactg ctgcttggct
     6541 aaatactcaa gcaatttctc atgggaaatc ggtcattcgt ctatccggtc ttttgctatc
     6601 aacggttgtc attccgataa ccccgtcatc tattcagata ttgtcgacta attcaatgtc
     6661 ctttcctaaa tcaccggatt tcattcggcg cttacgtgac tttactggta ctgattcaat
     6721 ttttaccgtg ggaactgcaa aagaacatag gtttctgcgt aatatcatct cgcctccact
     6781 tcgttacgat tacgttgtgt caaagctaca tgtcatcttc tttcgtgagg ttcggtcagt
     6841 tctacatagt tttccaagtg agcaagagaa tgacgacgac gtcgtaggtg tgatccgtac
     6901 agcaacattc cgtatcgtta tgcaagcatg ctttggattg gatacagtag aacattccaa
     6961 aattgatatg cttggtcatt tgtatcacaa ggctttatca cagccgtcct atatttcaat
     7021 tgcaaatcta ttgtttttac cgagttggtt cccgacatat tttttttcaa gttccgatgc
     7081 ctcgtcgtta cagctgcgca gagaagtttc gaagatttgt gatgatattt tgaaaaattt
     7141 gcatccagat agtaatccca tattttcaaa tgtgacgcaa gactcattgg tggctcatat
     7201 gattgatttt ttgaggtcag agccatcttc aaaatcagtt accctaacat ctgaaaaact
     7261 ggttggcacg gttctgtcat tcttatttgc ggggcaggca actactacat tggctattgc
     7321 atgggctttg catttactgt ctatcagtga tgatgtgcag aaagatctgc acactgaatt
     7381 acagagtata ggtgacagtt tggaagattt ggataggata ccgttgctag atggaattgt
     7441 aaaggagacg ttaagactat atcctccggt gcaatatata tctaggactg tgcataatcc
     7501 caaaaaagga ggagatataa tcgatggact gtttatacca aacggaacga cattgaaaat
     7561 acctacatta gcattacaga cacgatcaga cttgtggggc gacgatgcga atgaattttt
     7621 accgaagcgg tggatgaaag gagatggaaa acaggaagga gaaaactata aatggatgaa
     7681 atgggcattt atggcattct ggcatggttc atatggatgt ataggtcaga aattcgcact
     7741 tctagagatg aaagtattta tcaaggagat tgtaagcaaa tatgaggtca aacctgctct
     7801 ggttgtaagt gatgatggaa agtgcaaggt aaattcctca ccaaaaagat atggtttgag
     7861 tgaaccttac gggttgaaat tgttcttttc tccaagaata taa
//
