BGC0002673 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0002673 (Streptomyces griseus subsp. griseus NBRC 13350)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS	1	79,770	High	iminimycin A/iminimycin B	PKS+other:other

BGC0002673 - Region 1 - T1PKS

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Location: 1 - 79,770 nt. (total: 79,770 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 79,771
Label		BGC0002673

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase

SGR_6367: flavin reductase domain protein FMN-binding

smcogs: SMCOG1160: flavin reductase domain protein FMN-binding

SGR_6371: alcohol dehydrogenase

smcogs: SMCOG1040: alcohol dehydrogenase

SGR_6379: acyl-CoA dehydrogenase type 2

smcogs: SMCOG1191: acyl-CoA dehydrogenase type 2

Transferases: acylase aminotransferase

SGR_6373: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

SGR_6375: aminotransferase class-III

smcogs: SMCOG1013: aminotransferase class-III

Ligases: synthase

SGR_6370: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

SGR_6372: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002673.2	1.00	PKS, other (other)	iminimycin A, iminimycin B	Streptomyces griseus subsp. griseus NBRC 13350
BGC0001273.3	0.76	PKS	asperlactone	Aspergillus ochraceus
BGC0001276.3	0.75	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001275.3	0.75	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.75	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0002191.2	0.66	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001284.4	0.66	PKS	alternariol	Parastagonospora nodorum SN15
BGC0000041.3	0.64	PKS	oronofacic acid	Pseudomonas syringae
BGC0001164.3	0.64	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0002154.2	0.64	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002673.2	1.00	PKS, other (other)	iminimycin A, iminimycin B	Streptomyces griseus subsp. griseus NBRC 13350
BGC0001273.3	0.76	PKS	asperlactone	Aspergillus ochraceus
BGC0001276.3	0.75	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001275.3	0.75	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.75	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0002191.2	0.66	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001284.4	0.66	PKS	alternariol	Parastagonospora nodorum SN15
BGC0000041.3	0.64	PKS	oronofacic acid	Pseudomonas syringae
BGC0001164.3	0.64	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0002154.2	0.64	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 79770:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (Me-mal - Me-ccmal) + (ccmal - ccmal - ccmal) + (redmal - ccmal) + (ccmal)
Non-colinear ordering:: SGR_6373 + SGR_6372 + SGR_6371 + SGR_6370

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

SGR_6370: mal

PKS_AT (556..850): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 75.0%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 207.4
inactive: 99.0
Methoxymalonyl-CoA: 78.2
Methylmalonyl-CoA: 64.3
Propionyl-CoA: 54.0
Isobutyryl-CoA: 47.8
Ethylmalonyl-CoA: 32.6
Acetyl-CoA: 26.9
2-Methylbutyryl-CoA: 24.1
CHC-CoA: 24.0
trans-1,2-CPDA: 23.2
Benzoyl-CoA: 23.1
fatty_acid: 15.1
3-Methylbutyryl-CoA: 0.0

SGR_6371: mal - mal

PKS_AT (541..816): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 70.8%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 179.8
inactive: 90.8
Methoxymalonyl-CoA: 77.6
Methylmalonyl-CoA: 65.4
Propionyl-CoA: 46.5
Isobutyryl-CoA: 46.4
Ethylmalonyl-CoA: 27.4
Acetyl-CoA: 25.9
2-Methylbutyryl-CoA: 22.5
CHC-CoA: 20.6
trans-1,2-CPDA: 20.1
Benzoyl-CoA: 20.1
fatty_acid: 11.6
3-Methylbutyryl-CoA: 0.0

PKS_AT (2496..2762): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 70.8%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 182.5
inactive: 89.8
Methoxymalonyl-CoA: 79.9
Methylmalonyl-CoA: 66.5
Propionyl-CoA: 44.8
Isobutyryl-CoA: 37.6
Ethylmalonyl-CoA: 35.6
Acetyl-CoA: 29.6
2-Methylbutyryl-CoA: 23.1
CHC-CoA: 20.7
trans-1,2-CPDA: 20.4
Benzoyl-CoA: 17.2
fatty_acid: 10.2
3-Methylbutyryl-CoA: 0.0

SGR_6372: mal - mal - mal

PKS_AT (547..814): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 70.8%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 183.5
inactive: 84.5
Methoxymalonyl-CoA: 72.9
Methylmalonyl-CoA: 61.1
Propionyl-CoA: 47.0
Isobutyryl-CoA: 42.3
Acetyl-CoA: 29.0
Ethylmalonyl-CoA: 27.2
trans-1,2-CPDA: 24.0
CHC-CoA: 20.3
Benzoyl-CoA: 19.7
2-Methylbutyryl-CoA: 19.2
fatty_acid: 11.2
3-Methylbutyryl-CoA: 0.0

PKS_AT (2124..2395): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 70.8%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 184.2
inactive: 87.8
Methoxymalonyl-CoA: 73.2
Methylmalonyl-CoA: 60.1
Isobutyryl-CoA: 39.0
Propionyl-CoA: 35.7
trans-1,2-CPDA: 22.4
Ethylmalonyl-CoA: 21.5
Acetyl-CoA: 20.6
CHC-CoA: 19.4
Benzoyl-CoA: 16.3
2-Methylbutyryl-CoA: 14.4
fatty_acid: 11.9
3-Methylbutyryl-CoA: 0.0

PKS_AT (3696..3976): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 75.0%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 194.6
inactive: 99.4
Methoxymalonyl-CoA: 79.0
Methylmalonyl-CoA: 60.0
Isobutyryl-CoA: 35.8
Propionyl-CoA: 33.9
Ethylmalonyl-CoA: 23.0
CHC-CoA: 19.0
Acetyl-CoA: 18.0
trans-1,2-CPDA: 17.4
Benzoyl-CoA: 13.2
2-Methylbutyryl-CoA: 10.8
fatty_acid: 9.5
3-Methylbutyryl-CoA: 0.0

SGR_6373: mmal - mmal

PKS_AT (515..811): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 91.7%; Malonyl-CoA: 87.5%; Methoxymalonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 184.1
Ethylmalonyl-CoA: 175.8
Methoxymalonyl-CoA: 159.2
2-Methylbutyryl-CoA: 120.3
trans-1,2-CPDA: 106.4
Propionyl-CoA: 99.7
Malonyl-CoA: 86.9
CHC-CoA: 77.9
Isobutyryl-CoA: 59.2
3-Methylbutyryl-CoA: 43.1
fatty_acid: 40.5
Benzoyl-CoA: 34.4
inactive: 18.3
Acetyl-CoA: 17.8

PKS_AT (1537..1830): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 91.7%; Malonyl-CoA: 87.5%; Methoxymalonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 208.5
Ethylmalonyl-CoA: 198.5
Methoxymalonyl-CoA: 174.1
2-Methylbutyryl-CoA: 158.8
trans-1,2-CPDA: 127.5
Propionyl-CoA: 126.3
Malonyl-CoA: 115.7
CHC-CoA: 90.5
Isobutyryl-CoA: 70.9
Benzoyl-CoA: 58.5
Acetyl-CoA: 50.7
inactive: 49.4
fatty_acid: 41.2
3-Methylbutyryl-CoA: 24.7

Link to PARAS substrate predictor