BGC0002697 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0002697 (Streptantibioticus cattleyicolor NRRL 8057 = DSM 46488)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	T1PKS,NRPS	1	41,791	clipibicyclene/azabicyclene B/azabicyclene C/azabicyclene D	NRPS:Type I+PKS	100%

BGC0002697 - Region 1 - NRPS,T1PKS

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Location: 1 - 41,791 nt. (total: 41,791 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS: cds(Condensation and (AMP-binding or A-OX))

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core biosynthetic genes

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transport-related genes

regulatory genes

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Gene details

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Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 41,792
Label		BGC0002697

Detailed domain annotation

Selected features only

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Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002697	1.55	NRP, Polyketide	clipibicyclene, azabicyclene B, azabicyclene C, azabicyclene D	Streptomyces cattleya NRRL 8057 = DSM 46488
BGC0001873	0.85	NRP	pyrrolizixenamide A	Xenorhabdus szentirmaii DSM 16338
BGC0002075	0.64	NRP, Alkaloid	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0001286	0.58	Other	itaconic acid	Ustilago maydis
BGC0000457	0.57	NRP	vicibactin	Rhizobium etli CFN 42
BGC0000894	0.57	Other	citrinin	Monascus aurantiacus
BGC0001185	0.56	NRP	bacillibactin	Bacillus velezensis FZB42
BGC0001833	0.55	NRP	icosalide A, icosalide B	Burkholderia gladioli
BGC0000417	0.55	NRP	rhodochelin	Rhodococcus jostii RHA1
BGC0001132	0.55	NRP	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002697	1.00	NRP, Polyketide	clipibicyclene, azabicyclene B, azabicyclene C, azabicyclene D	Streptomyces cattleya NRRL 8057 = DSM 46488
BGC0001873	0.73	NRP	pyrrolizixenamide A	Xenorhabdus szentirmaii DSM 16338
BGC0000138	0.67	Polyketide	rimocidin	Streptomyces diastaticus
BGC0000894	0.66	Other	citrinin	Monascus aurantiacus
BGC0000996	0.66	NRP, Polyketide	frontalamide B, frontalamide A	Streptomyces sp. SPB78
BGC0002365	0.66	Other, Polyketide	10-epi-HSAF, 10-epi-3-deOH-HSAF, 10-epi-maltophilin, 10-epi-xanthobaccin C, 10-epi-hydroxymaltophilin, 10-epi-FI-2	Streptomyces sp.
BGC0001043	0.65	NRP, Polyketide	SGR PTMs, SGR PTM Compound b, SGR PTM Compound c, SGR PTM Compound d	Streptomyces griseus subsp. griseus NBRC 13350
BGC0001286	0.65	Other	itaconic acid	Ustilago maydis
BGC0002075	0.65	NRP, Alkaloid	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0001185	0.64	NRP	bacillibactin	Bacillus velezensis FZB42

Similar known gene clusters from MIBiG 4.0

Module view

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 41791:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal - ccmal - Me-ccmal - ccmal) + (Ser - X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 0 - 35374:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal - ccmal - Me-ccmal - ccmal) + (Ser - X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

SCATT_32630

Search NORINE for peptide: strict or relaxed

AMP-binding (434..839): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: DVWHFSLVDK
Nearest Stachelhaus code(s):: Ser DVWHFSLVDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1498..1900): X

nrpys: Aze

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
Small clusters prediction:: pro (Pro)
Single AA prediction:: pro (Pro)

Stachelhaus prediction details:

Stachelhaus sequence:: DVQFISQIAK
Nearest Stachelhaus code(s):: Aze DVQFISQIAK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

SCATT_32680

PKS_AT (541..839): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 70.8%; inactive: 58.3%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 176.4
inactive: 79.3
Methylmalonyl-CoA: 73.6
Methoxymalonyl-CoA: 69.5
Propionyl-CoA: 42.2
Ethylmalonyl-CoA: 40.6
2-Methylbutyryl-CoA: 33.2
Acetyl-CoA: 31.2
Benzoyl-CoA: 30.7
Isobutyryl-CoA: 25.0
CHC-CoA: 21.4
fatty_acid: 15.3
trans-1,2-CPDA: 12.1
3-Methylbutyryl-CoA: 7.8

PKS_AT (1560..1851): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 79.2%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 185.1
inactive: 85.9
Methoxymalonyl-CoA: 64.8
Methylmalonyl-CoA: 58.9
Propionyl-CoA: 37.8
Isobutyryl-CoA: 32.4
fatty_acid: 25.3
CHC-CoA: 24.5
Ethylmalonyl-CoA: 22.1
Acetyl-CoA: 15.9
2-Methylbutyryl-CoA: 11.8
trans-1,2-CPDA: 7.4
Benzoyl-CoA: 5.3
3-Methylbutyryl-CoA: 0.0

PKS_AT (3263..3565): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 222.9
Ethylmalonyl-CoA: 222.9
Methoxymalonyl-CoA: 190.9
2-Methylbutyryl-CoA: 145.3
trans-1,2-CPDA: 133.7
Propionyl-CoA: 124.2
CHC-CoA: 101.8
Malonyl-CoA: 100.9
Isobutyryl-CoA: 66.3
Benzoyl-CoA: 59.3
fatty_acid: 44.7
Acetyl-CoA: 43.0
3-Methylbutyryl-CoA: 33.2
inactive: 27.1

PKS_AT (4972..5255): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 79.2%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 182.6
inactive: 89.5
Methylmalonyl-CoA: 73.3
Methoxymalonyl-CoA: 72.0
Propionyl-CoA: 52.3
Isobutyryl-CoA: 34.0
Ethylmalonyl-CoA: 33.6
2-Methylbutyryl-CoA: 27.4
fatty_acid: 27.3
Acetyl-CoA: 26.6
CHC-CoA: 24.2
Benzoyl-CoA: 7.2
trans-1,2-CPDA: 2.3
3-Methylbutyryl-CoA: 1.5