BGC0002697 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0002697 (Streptantibioticus cattleyicolor NRRL 8057 = DSM 46488)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	NRPS,T1PKS	1	69,108	High	clipibicyclene/azabicyclene B/azabicyclene C/azabicyclene D	NRPS:Type I+PKS

BGC0002697 - Region 1 - NRPS,T1PKS

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Location: 1 - 69,108 nt. (total: 69,108 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 69,109
Label		BGC0002697

Tailoring functions

Oxidoreductases: dehydrogenase oxygenase

SCATT_32610: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

SCATT_32620: cytochrome P450

smcogs: SMCOG1034: cytochrome P450

SCATT_32660: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Ligases: ligase synthase

SCATT_32630: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

SCATT_32650: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

SCATT_32680: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002697.3	1.53	NRPS, PKS	clipibicyclene, azabicyclene B, azabicyclene C, azabicyclene D	Streptomyces cattleya NRRL 8057 = DSM 46488
BGC0002075.3	0.85	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0001873.3	0.79	NRPS	pyrrolizixenamide A	Xenorhabdus szentirmaii DSM 16338
BGC0001833.4	0.75	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001132.4	0.75	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001825.4	0.72	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001128.4	0.72	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002286.3	0.71	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0000342.4	0.70	NRPS	enniatin	Fusarium equiseti
BGC0001758.4	0.69	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002697.3	0.99	NRPS, PKS	clipibicyclene, azabicyclene B, azabicyclene C, azabicyclene D	Streptomyces cattleya NRRL 8057 = DSM 46488
BGC0001273.3	0.75	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	0.75	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.75	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0002075.3	0.74	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0001873.3	0.71	NRPS	pyrrolizixenamide A	Xenorhabdus szentirmaii DSM 16338
BGC0001833.4	0.70	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001132.4	0.70	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001825.4	0.69	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001128.4	0.69	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 69108:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal - ccmal - Me-ccmal - ccmal) + (Ser - X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 0 - 47155:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal - ccmal - Me-ccmal - ccmal) + (Ser - X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 11255 - 69108:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal - ccmal - Me-ccmal - ccmal) + (Ser - X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

SCATT_32630: Ser - X

Search NORINE for peptide: strict or relaxed

AMP-binding (434..839): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: DVWHFSLVDK
Nearest Stachelhaus code(s):: Ser DVWHFSLVDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1498..1900): X

nrpys: Aze

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
Small clusters prediction:: pro (Pro)
Single AA prediction:: pro (Pro)

Stachelhaus prediction details:

Stachelhaus sequence:: DVQFISQIAK
Nearest Stachelhaus code(s):: Aze DVQFISQIAK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

SCATT_32680: mal - mal - mmal - mal

PKS_AT (541..839): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 70.8%; inactive: 58.3%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 176.4
inactive: 79.3
Methylmalonyl-CoA: 73.6
Methoxymalonyl-CoA: 69.5
Propionyl-CoA: 42.2
Ethylmalonyl-CoA: 40.6
2-Methylbutyryl-CoA: 33.2
Acetyl-CoA: 31.2
Benzoyl-CoA: 30.7
Isobutyryl-CoA: 25.0
CHC-CoA: 21.4
fatty_acid: 15.3
trans-1,2-CPDA: 12.1
3-Methylbutyryl-CoA: 7.8

PKS_AT (1560..1851): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 79.2%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 185.1
inactive: 85.9
Methoxymalonyl-CoA: 64.8
Methylmalonyl-CoA: 58.9
Propionyl-CoA: 37.8
Isobutyryl-CoA: 32.4
fatty_acid: 25.3
CHC-CoA: 24.5
Ethylmalonyl-CoA: 22.1
Acetyl-CoA: 15.9
2-Methylbutyryl-CoA: 11.8
trans-1,2-CPDA: 7.4
Benzoyl-CoA: 5.3
3-Methylbutyryl-CoA: 0.0

PKS_AT (3263..3565): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 222.9
Ethylmalonyl-CoA: 222.9
Methoxymalonyl-CoA: 190.9
2-Methylbutyryl-CoA: 145.3
trans-1,2-CPDA: 133.7
Propionyl-CoA: 124.2
CHC-CoA: 101.8
Malonyl-CoA: 100.9
Isobutyryl-CoA: 66.3
Benzoyl-CoA: 59.3
fatty_acid: 44.7
Acetyl-CoA: 43.0
3-Methylbutyryl-CoA: 33.2
inactive: 27.1

PKS_AT (4972..5255): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 79.2%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 182.6
inactive: 89.5
Methylmalonyl-CoA: 73.3
Methoxymalonyl-CoA: 72.0
Propionyl-CoA: 52.3
Isobutyryl-CoA: 34.0
Ethylmalonyl-CoA: 33.6
2-Methylbutyryl-CoA: 27.4
fatty_acid: 27.3
Acetyl-CoA: 26.6
CHC-CoA: 24.2
Benzoyl-CoA: 7.2
trans-1,2-CPDA: 2.3
3-Methylbutyryl-CoA: 1.5

Link to PARAS substrate predictor