LOCUS       BGC0002701              9977 bp    DNA     linear   CON 23-APR-2023
DEFINITION  Paenibacillus thiaminolyticus strain NRRL B-4156 Ga0138519_137,
            whole genome shotgun sequence.
ACCESSION   BGC0002701
VERSION     BGC0002701
KEYWORDS    .
SOURCE      Paenibacillus thiaminolyticus
  ORGANISM  Paenibacillus thiaminolyticus
            Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae;
            Paenibacillus.
COMMENT     REFSEQ INFORMATION: The reference sequence is identical to
            NDGK01000036.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 18:15:03
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 9977
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             complement(1..1761)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0042626 - ATPase-coupled transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="resistance (resist) ABC_efflux (Score:
                     249.7; E-value: 3.7e-76)"
                     /gene_functions="transport (smcogs) SMCOG1288:ABC
                     transporter related protein (Score: 342.3; E-value:
                     8.7e-104)"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011248399.1"
                     /locus_tag="B7751_RS20850"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_087443883.1"
                     /transl_table=11
                     /translation="MKRLTLKQFIGILDKYRPSGWLLFIALVLSIIQTGISLIIPLVSM
                     ELVDLLVVEEFNIFTLLGLLLLFMFQVSLSGISLYMMIYIGEGIIVRLREDLWRRVVRF
                     PVKFFDANNSGEIMSRITNDTMVMKNFFVEHLIPFFTGLITITGSLFILFLIDWKMALI
                     FLLVFPAAFLVLNPLGKKMYRVSKDVQTETALFQGNLSRVLSDVRLVKLSVAENEEAVQ
                     GAKRARRLYKYGLESGKVIAVVSPLITTTLLIVLVGIFGYGGYQVATGSLSAGALVAIV
                     FYIFQIMTPVTLMAQFFTQAQKAMGATERVNSLLVEELEEVVVSNKENVKEDIEAGITF
                     SNVVFSYSEKGEVLNNISFTAKEGKKTAIVGESGVGKTTLFSLLERFYIVDSGDIFYNG
                     RSIYSHELSEWREKIAYVSQDTPIMEGSIKDNLVYGIKKDISDSIIFDALKSADLDSFV
                     SSLPQGLDTEVGEKGIRLSGGQKQRIAIARAIIRDPKILLLDEATAHLDSQSEALVQEA
                     FNNLMRDRTTIIIAHRLSTIKDADCIVVIQEGKVSGCGSHYELYETNQLYKNLFNQQTF
                     SKELVLSGNV"
     protocluster    1..9977
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[4187:6794]"
                     /cutoff="20000"
                     /detection_rule="(micKC and not PKS_KS)"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-iii"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      4188..6794
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(micKC and not PKS_KS)"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-iii"
                     /protocluster_number="1"
     cand_cluster    1..9977
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(micKC and not PKS_KS)"
                     /kind="single"
                     /product="lanthipeptide-class-iii"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..9977
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0002701"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..9977
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="lanthipeptide-class-iii"
                     /region_number="1"
                     /rules="(micKC and not PKS_KS)"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            complement(1..1761)
                     /locus_tag="B7751_RS20850"
     gene            complement(1939..2781)
                     /locus_tag="B7751_RS20855"
     CDS             complement(1939..2781)
                     /EC_number="2.1.1.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1248:methyltransferase (Score: 70.2; E-value:
                     3.2e-21)"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /locus_tag="B7751_RS20855"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="WP_087443884.1"
                     /transl_table=11
                     /translation="MINIEKLLEIAKNPEPFEEGTQEIWLDPDRADLVLKGHFDENIPG
                     GSRDSSFIDETIDFINNLAPVEKYKKVIDLGCGPGLYSQRLAMRGYDVVGVDFNQNSID
                     YAIREAQEKNLSIDYRNEDITNIDLENDFDLALLIYQIYGVFNPTKRKKILSNIHRGLK
                     PGGLVLLDVLSDTSYEKFEPKIVWVLSNEGSIFSDKSHLTLYAALKYPNKVTVERSILV
                     FDDGELVNYNYWNQNFTIEDLEKEADEAGFTLEKVYADVNGGEYVNNSESFAAVLKKK"
     gene            complement(2947..3075)
                     /locus_tag="B7751_RS30335"
     CDS             complement(2947..3075)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (lanthipeptides)
                     predicted lanthipeptide"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF038154.3"
                     /locus_tag="B7751_RS30335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="class III lanthipeptide"
                     /protein_id="WP_187144228.1"
                     /transl_table=11
                     /translation="MNAVLELQKLAQATDGKGQAAEATITIITVGKSTLSVYQCNS"
     CDS_motif       complement(3010..3075)
                     /prepeptide="leader"
                     /note="peptide class: {self.peptide_class}"
                     /note="predicted sequence: MNAVLELQKLAQATDGKGQAAE"
                     /locus_tag="B7751_RS30335_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       complement(2947..3009)
                     /prepeptide="core"
                     /locus_tag="B7751_RS30335_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class III"
                     /score="2.00"
                     /molecular_weight="1973.3"
                     /monoisotopic_mass="1972.1"
                     /core_sequence="ATITIITVGKSTLSVYQCNS"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MNAVLELQKLAQATDGKGQAAE"
                     /leader_location="[3009:3075](-)"
                     /alternative_weights="1991.3"
                     /alternative_weights="2009.3"
                     /alternative_weights="2027.3"
                     /alternative_weights="2045.3"
                     /alternative_weights="2063.4"
                     /alternative_weights="2081.4"
     gene            complement(3109..3234)
                     /locus_tag="B7751_RS30340"
     CDS             complement(3109..3234)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (lanthipeptides)
                     predicted lanthipeptide"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF038154.3"
                     /locus_tag="B7751_RS30340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="class III lanthipeptide"
                     /protein_id="WP_187144229.1"
                     /transl_table=11
                     /translation="MNAVLELQKLAQATDGKGQAAEATITIITVGKSTLSVYKCN"
     CDS_motif       complement(3169..3234)
                     /prepeptide="leader"
                     /note="peptide class: {self.peptide_class}"
                     /note="predicted sequence: MNAVLELQKLAQATDGKGQAAE"
                     /locus_tag="B7751_RS30340_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       complement(3109..3168)
                     /prepeptide="core"
                     /locus_tag="B7751_RS30340_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class III"
                     /score="2.00"
                     /molecular_weight="1904.3"
                     /monoisotopic_mass="1903.1"
                     /core_sequence="ATITIITVGKSTLSVYKCN"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MNAVLELQKLAQATDGKGQAAE"
                     /leader_location="[3168:3234](-)"
                     /alternative_weights="1922.3"
                     /alternative_weights="1940.3"
                     /alternative_weights="1958.3"
                     /alternative_weights="1976.3"
                     /alternative_weights="1994.4"
     gene            complement(3268..3393)
                     /locus_tag="B7751_RS30345"
     CDS             complement(3268..3393)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (lanthipeptides)
                     predicted lanthipeptide"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF038154.3"
                     /locus_tag="B7751_RS30345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="class III lanthipeptide"
                     /protein_id="WP_187144229.1"
                     /transl_table=11
                     /translation="MNAVLELQKLAQATDGKGQAAEATITIITVGKSTLSVYKCN"
     CDS_motif       complement(3328..3393)
                     /prepeptide="leader"
                     /note="peptide class: {self.peptide_class}"
                     /note="predicted sequence: MNAVLELQKLAQATDGKGQAAE"
                     /locus_tag="B7751_RS30345_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       complement(3268..3327)
                     /prepeptide="core"
                     /locus_tag="B7751_RS30345_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class III"
                     /score="2.00"
                     /molecular_weight="1904.3"
                     /monoisotopic_mass="1903.1"
                     /core_sequence="ATITIITVGKSTLSVYKCN"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MNAVLELQKLAQATDGKGQAAE"
                     /leader_location="[3327:3393](-)"
                     /alternative_weights="1922.3"
                     /alternative_weights="1940.3"
                     /alternative_weights="1958.3"
                     /alternative_weights="1976.3"
                     /alternative_weights="1994.4"
     gene            complement(3408..3563)
                     /locus_tag="B7751_RS30110"
     CDS             complement(3408..3563)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (lanthipeptides)
                     predicted lanthipeptide"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF038154.3"
                     /locus_tag="B7751_RS30110"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="class III lanthipeptide"
                     /protein_id="WP_167386828.1"
                     /transl_table=11
                     /translation="MNAVLELQKLAHDTDGKGQADVIPTITTITTTRWSLTSNFLCRNE
                     SQIKII"
     CDS_motif       complement(3501..3563)
                     /prepeptide="leader"
                     /note="peptide class: {self.peptide_class}"
                     /note="predicted sequence: MNAVLELQKLAHDTDGKGQAD"
                     /locus_tag="B7751_RS30110_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       complement(3408..3500)
                     /prepeptide="core"
                     /locus_tag="B7751_RS30110_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class III"
                     /score="0.50"
                     /molecular_weight="3270.8"
                     /monoisotopic_mass="3268.9"
                     /core_sequence="VIPTITTITTTRWSLTSNFLCRNESQIKII"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MNAVLELQKLAHDTDGKGQAD"
                     /leader_location="[3500:3563](-)"
                     /alternative_weights="3288.8"
                     /alternative_weights="3306.8"
                     /alternative_weights="3324.9"
                     /alternative_weights="3342.9"
                     /alternative_weights="3360.9"
                     /alternative_weights="3378.9"
                     /alternative_weights="3396.9"
                     /alternative_weights="3415.0"
                     /alternative_weights="3433.0"
     gene            complement(3600..3740)
                     /locus_tag="B7751_RS30115"
     CDS             complement(3600..3740)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (lanthipeptides)
                     predicted lanthipeptide"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF038154.3"
                     /locus_tag="B7751_RS30115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="class III lanthipeptide"
                     /protein_id="WP_164776404.1"
                     /transl_table=11
                     /translation="MNAVLELQKLAHDTVGKGQAADVIPTITTITNTTRWSLTSNFLCR
                     Q"
     CDS_motif       complement(3675..3740)
                     /prepeptide="leader"
                     /note="peptide class: {self.peptide_class}"
                     /note="predicted sequence: MNAVLELQKLAHDTVGKGQAAD"
                     /locus_tag="B7751_RS30115_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       complement(3600..3674)
                     /prepeptide="core"
                     /locus_tag="B7751_RS30115_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class III"
                     /score="1.30"
                     /molecular_weight="2605.0"
                     /monoisotopic_mass="2603.5"
                     /core_sequence="VIPTITTITNTTRWSLTSNFLCRQ"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MNAVLELQKLAHDTVGKGQAAD"
                     /leader_location="[3674:3740](-)"
                     /alternative_weights="2623.0"
                     /alternative_weights="2641.0"
                     /alternative_weights="2659.0"
                     /alternative_weights="2677.1"
                     /alternative_weights="2695.1"
                     /alternative_weights="2713.1"
                     /alternative_weights="2731.1"
                     /alternative_weights="2749.1"
     gene            complement(3870..4016)
                     /locus_tag="B7751_RS30120"
     CDS             complement(3870..4016)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (lanthipeptides)
                     predicted lanthipeptide"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF038154.3"
                     /locus_tag="B7751_RS30120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="class III lanthipeptide"
                     /protein_id="WP_164776402.1"
                     /transl_table=11
                     /translation="MNAVLELQKLAHNTDGKGQAIDVTPTTTVFPPPIYSTVSVSAWEC
                     YKN"
     CDS_motif       complement(3951..4016)
                     /prepeptide="leader"
                     /note="peptide class: {self.peptide_class}"
                     /note="predicted sequence: MNAVLELQKLAHNTDGKGQAID"
                     /locus_tag="B7751_RS30120_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       complement(3870..3950)
                     /prepeptide="core"
                     /locus_tag="B7751_RS30120_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class III"
                     /score="-1.90"
                     /molecular_weight="2744.1"
                     /monoisotopic_mass="2742.4"
                     /core_sequence="VTPTTTVFPPPIYSTVSVSAWECYKN"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MNAVLELQKLAHNTDGKGQAID"
                     /leader_location="[3950:4016](-)"
                     /alternative_weights="2762.1"
                     /alternative_weights="2780.1"
                     /alternative_weights="2798.1"
                     /alternative_weights="2816.2"
                     /alternative_weights="2834.2"
                     /alternative_weights="2852.2"
                     /alternative_weights="2870.2"
     gene            complement(4188..6794)
                     /gene="lanKC"
                     /locus_tag="B7751_RS20860"
     CDS             complement(4188..6794)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0004672 - protein kinase activity [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]; GO:0006468 - protein phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="lanKC"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-iii: micKC"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Pkinase"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) LANC_like"
                     /gene_functions="regulatory (smcogs)
                     SMCOG1030:serine/threonine protein kinase (Score: 84.3;
                     E-value: 1.7e-25)"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF038151.1"
                     /locus_tag="B7751_RS20860"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="class III lanthionine synthetase LanKC"
                     /protein_id="WP_164776400.1"
                     /sec_met_domain="micKC (E-value: 7.3e-138, bitscore: 450.3,
                     seeds: 18, tool: rule-based-clusters)"
                     /sec_met_domain="Pkinase (E-value: 1.5e-22, bitscore: 69.7,
                     seeds: 54, tool: rule-based-clusters)"
                     /sec_met_domain="LANC_like (E-value: 8.4e-16, bitscore:
                     47.1, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEGNMLYHQYMKPDSEYYEKMSAIEKAYEVNEIPDTYDVISDSDS
                     VWKHYHFKGLTLPEQGWKIHVTATFEDSQSVLEKVARLCIDRKIEFKHLKDRNSFIEMN
                     SKNANRASSGKFITIYPINNESFVELLELISSTIQDFQKGPYILNDKRWKNSNVFYRYG
                     GFKTIINEHGEHCIRDEQGNLIKDQRTPFYQVPDFVKDFDDYLNTINNDLETKAEGNLE
                     KYDIETALTYSNAGGVYRATRKKDNMKVIIKEARPNAGLDGAGQDALSRQKIEYDALKK
                     LKDVPGVVNLIEYFQEWEHYFLVEEFIEGQDLRQWIARTFPFVKDSELIRDHAKNVKKI
                     LLQLFPLIDHMHQNGVAMGDLQPSNVMVTEDLTVRIIDFETAMPVNSEDNPSMATTGFV
                     SREMKVSGARDWFGLKKIVMFLVLPVLSSEDLEGYLQSNHHNWIKENYGDSFYRFIVDL
                     QEKCNKRINDYQQYTPKEINLSDQSSDFNISSIIYKLIKGIENNLTNDERFINGDIRQF
                     MMSGGKFNFLTGGSGTAFTLTKQQSKTVEVDNWIQSFLLDNLARIEDNGLLTGKTGILA
                     LLYAKGYKEVVFNEVRVLKDNINETNISLRSGLSGIGLFVISLYLETENKEYLQFAIEI
                     EELIDFNRVKGEPLRVNDWMAVDIGAIDGLSGVSLFYSALYSATNNKKYLEKAELVLKE
                     DLESTKKDDVSGVLQTLDKRNRLLPYLSGGSIGIAISIWFLNHVSGQDLYREEMDAVSK
                     LSKICCTISGSLFDGAGSFLLIPSMMEHGENREEIVSEVLKLLNLFLIEKNGYYVYPGQ
                     FSFRLADDVYTGSSGIILALMGVVKDNPLYWLPLINSDEFLEKTKAKALVVTSEQR"
     gene            complement(7414..8697)
                     /locus_tag="B7751_RS20865"
     CDS             complement(7414..8697)
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_M16"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_M16_C"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002150927.1"
                     /locus_tag="B7751_RS20865"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="pitrilysin family protein"
                     /protein_id="WP_087443886.1"
                     /sec_met_domain="Peptidase_M16 (E-value: 3.5e-20, bitscore:
                     62.1, seeds: 24, tool: rule-based-clusters)"
                     /sec_met_domain="Peptidase_M16_C (E-value: 5.6e-21,
                     bitscore: 64.9, seeds: 139, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MESMVYKRVQETIYYEKLTNGLEVFILPKKGFHKTFATFTTKYGS
                     VDNKFSTPGNNCATQVPDGIAHFLEHKMFESEKVDVFHTFAKQGAFVNAFTSFTRTAYT
                     FSATSNINNNLITLLDFVQEPYFTDENVEKEKGIIEQEIKMYQDSPDWRARFGILENMY
                     KSHPVKIDIAGTITSINQITKEDLFTCYNTFYHPNNMLLFVIGPVVPEEVIRLIRSNQN
                     QKTFREPTEIHRIYPEEEAKVNRRSSIMKMPVETPKVLIGFKESNPKRQGQDMLKYELS
                     LNVLLELMFGNSSEAYEKMYNNGYLDETFTFDYTNEQGFGFSVIGGNSQEPEKLINIVD
                     ETIVGFKKKSIKKEDVQRVIKKEMGNFLRAINSPQFIANQFTRYRFNKMDLFEILPTLE
                     NLIEKELEEVLHQHFSDISRTTLIVNGD"
     gene            complement(8703..9977)
                     /locus_tag="B7751_RS20870"
     CDS             complement(8703..9977)
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_M16_C"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002150929.1"
                     /locus_tag="B7751_RS20870"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="pitrilysin family protein"
                     /protein_id="WP_087443887.1"
                     /sec_met_domain="Peptidase_M16_C (E-value: 9.6e-27,
                     bitscore: 83.7, seeds: 139, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDEIAIRSSEQNGLHLHLCNTDKFKTISIMLTMKSPLQKDLITAR
                     ALIPHILNGGSKNYPHHKLIKQKLDEMYGATLSPDVKKEGDNHIISFRMEIPAAQYLNV
                     TNALLESALDLLHDIVYSPVLENGVFNSSIVEQEKRSLKQRLASMYNEKMLYTSVRLIE
                     EMFEGEPYQLPAYGSEKDIDSLSDLSIYQIYEDLLQNDRFDLYIVGAFNEDEVKLMVHD
                     VFSEQHNNKPVNTISPARKDRNGIKIIQDKQDVNQGKLLLGYRTYSTIQDEDFEATRLA
                     NAIFGRYPSSKLFINVREKKSLAYFVFSQLESNKGLLLTMAGIDFDNYERAVEIIREQE
                     YAMKEGNFTEDEVEQGKAMLINLLLEAYDTPLGIIDISMEAVDSGFSSNIVDQINRINR
                     VSKSDVIRAANKWELDTIYFLNRKE"
ORIGIN
        1 tcacacattc ccactaagaa ccaattcttt agaaaatgtt tgctgattaa ataagttctt
       61 atataactga tttgtttcat ataattcgta atgtgaacca caccctgata ctttaccttc
      121 ctgtataact acaatacagt ctgcatcctt aatagttgaa agacggtgtg caataattat
      181 cgttgttcta tctctcatta aattattgaa agcttcttga acgagtgcct cagattgact
      241 atctaaatga gctgtagctt catctaatag tagtattttt ggatctctta tgatggctct
      301 agcaattgct attctttgtt tttgtccacc tgataagcgg ataccttttt caccaacctc
      361 cgtatctagt ccttgaggca gtgatgacac aaaagagtct aagtctgcac tctttagtgc
      421 atcaaaaata atggaatcac ttatatcttt ctttatgcca tacactaaat tgtccttaat
      481 actgccttcc ataatagggg tatcctggct aacgtatgca atcttctctc tccattcact
      541 taattcatga gaatatatcg atctaccatt ataaaaaata tccccactgt caacaatata
      601 gaatcgttca agtaatgaga ataaagttgt tttaccgact ccactttcac ccacaattgc
      661 cgtcttttta ccttccttag ctgtaaagct aatattattt aatacttctc ccttttcact
      721 ataagaaaat acaacatttg agaaggtaat tccagcttca atgtcttctt tgacattttc
      781 tttgttggat acaacaactt cttcaagctc ttctactaat aatgaattga cacgttctgt
      841 tgctcccatt gctttttggg cttgagtgaa aaattgagcc attaatgtta caggagtcat
      901 aatttggaat atataaaata ctatagctac taaagctcct gctgacaaag agcctgttgc
      961 aacttgatag cctccataac caaatatgcc aactaataca ataaggagcg ttgttgtaat
     1021 aagaggtgat actactgcta ttactttccc agattctaag ccatatttat ataatcttct
     1081 tgcgcgctta gctccttgaa cagcttcttc attttcagca actgaaagct ttactaaacg
     1141 aacatcactt aataccctgc taagatttcc ttgaaataag gctgtttcag tttgcacatc
     1201 tttggaaacc ctgtacatct ttttaccaag tgggtttaga accaaaaatg cagcaggaaa
     1261 aactagtaaa aaaataagcg ccatcttcca atctattaga aaaagaatga aaagagaacc
     1321 tgtaatagta attagccctg taaagaaagg aatcaaatgc tctacaaaga aattcttcat
     1381 taccatagta tcatttgtaa ttctactcat tatttctcct gaattatttg catcaaaaaa
     1441 tttaacagga aatcttacta ctcttctcca taaatcttct cttaggcgga caattattcc
     1501 ttcaccaata taaatcatca tataaagaga aataccagaa agtgaaactt ggaacataaa
     1561 aagaagtaac aagcctaata aagtgaaaat gttaaattct tctacaacca ataaatcgac
     1621 aagttccatg gaaactaatg gaattattaa agatataccc gtttgaatta tcgataatac
     1681 aagtgctatg aaaagtaacc acccagaagg tctgtactta tctagaatac cgataaattg
     1741 ttttaaagtg agtcttttca tattttatgt ttatcctttt atttttattt ttgaacgact
     1801 tatttaaaca catattaaat atgtgaaact ttcatatcct tagacatttt acctttagga
     1861 ttcagtgtta gagccagggg attaaattat tcctattctt gcaaacactg aatccttaaa
     1921 tgggattttc ttatctgatt atttcttttt taaaactgcg gcgaaggact cgctattgtt
     1981 gacgtattct ccaccattaa catctgcata taccttttca agagtaaatc ctgcttcatc
     2041 cgcttctttt tctaaatctt caatagtgaa attttgattc caataattat aatttactaa
     2101 ctcaccatca tcaaaaacta gaatacttct ttccacagta actttattag gatacttaag
     2161 tgccgcgtat aaagttaaat ggctcttatc tgaaaatatg ctaccttcat tagataatac
     2221 ccacacaatt tttggttcga acttttcata actagtgtct gataacacgt ctaataatac
     2281 taatcctccc ggttttaacc cacggtggat attgctaagt attttttttc tcttagttgg
     2341 attaaacaca ccataaatct gataaattaa aagggctaaa tcaaaatcat tttctaggtc
     2401 aatattagta atatcttcat tcctataatc tattgataaa ttcttttctt gagcttccct
     2461 aattgcgtag tcaatggaat tttggttaaa atctacacct acaacatcgt aacccctcat
     2521 tgctaatctt tgtgaataga gccctggacc acaacctaag tcaataacct ttttatattt
     2581 ctctactggt gcaaggttat taataaaatc aatagtttca tcaataaaac tgctatccct
     2641 gctaccacca ggtatattct cgtcaaagtg acccttcaaa acaagatcag ctctatctgg
     2701 atcaagccag atttcttgtg tgccctcctc aaatggctcg gggtttttgg ctatttctaa
     2761 taacttttcg atatttatca ccctctcttg attttttcat gatttttgag tataatgagt
     2821 gaattctggg tgcttcgctg aactccactc aactaagcac cccagaatta ttttagtaat
     2881 tgttacttgc tttgatcaaa aattgaactt ttgcattcag ttaaatccta tttaattttt
     2941 gaagattcat gaattgcact gataaacact tagtgtcgac tttcctacag ttatgattgt
     3001 gattgttgct tccgctgctt ggcctttacc atcagtggct tgcgctaatt tttgcaattc
     3061 caatactgcg ttcattttct tcacctcctt taaataattt ggctttcatt aattgcactt
     3121 ataaacactt aaggttgact ttcctacagt tatgattgtg attgttgctt ccgctgcttg
     3181 gcctttacca tcagtggctt gcgctaattt ttgcaattcc aatactgcgt tcattttctt
     3241 cacctccttt aaataatttg gctttcatta attgcactta taaacactta aggttgactt
     3301 tcctacagtt atgattgtga ttgttgcttc cgctgcttgg cctttaccat cagtggcttg
     3361 cgctaatttt tgcaattcca atactgcgtt catttttttc acctccttta aataatctta
     3421 atttggcttt cattgcggca caaaaaatta cttgttagcg accacctagt agttgtaatc
     3481 gttgttattg tgggtattac atcagcttgg cctttaccat cagtgtcatg cgctaatttt
     3541 tgtaattcca atacggcgtt catttttttc acctccttta aataatctta atttggcttt
     3601 cattggcggc acaaaaaatt acttgttagc gaccacctag tagtatttgt aatcgttgtt
     3661 attgtgggta ttacatccgc tgcttgacct ttaccaactg tatcatgcgc taatttttgt
     3721 aattccaata cggcgttcat tttttcacct cctttaaata atttggcttt gattacttgg
     3781 tgtcgaccaa caagtatctg atattgttct tgtaattgtt attactggtg ttacatccgc
     3841 tggttggcct ttaccatctg tgtcatgcgc taatttttgt aacactccca agcactaaca
     3901 ctaacagttg aataaatagg cggtggaaat acagtcgttg ttggtgttac atctattgct
     3961 tggcctttac catctgtgtt atgcgctaat ttttgtaatt ccaatacggc gttcattttt
     4021 tcacctccat taaattaatt tgactttcat tctagcctta ccgaaaaact ttggtgtcga
     4081 ccaaatagtt gctagagtcg ttattattgt tgtgactgtt gcatcctccg cttggccttt
     4141 accatcagtg tcatgcgctt attttgcaag ctaaacatgt aatcatatca ccgttgttca
     4201 ctagtgacta ctagagcctt ggcctttgtt ttttctagaa attcatccga attaatcaat
     4261 ggtaaccaat agagtggatt atctttaact acccccatta atgctaaaat tattcctgaa
     4321 cttcctgtat aaacatcatc ggctaatcta aaagaaaatt gtcctgggta tacataataa
     4381 ccattcttct caattaaaaa gagattaagt aacttcaata cctcactaac aatttcctcg
     4441 cgattttcac catgttccat catagaaggg attaaaagaa aactacctgc gccatcaaat
     4501 aagcttccac taattgtaca acaaatcttt gataattttg aaaccgcatc catctcttct
     4561 ctataaaggt cttgtccact gacatgattt aaaaaccaaa ttgaaatagc aattccaata
     4621 gatcctccag aaagataagg caataggcga tttctcttat ccaatgtttg taatacacca
     4681 cttacatcat ctttttttgt tgattctaaa tcttccttta gtactaactc agccttttct
     4741 agatacttct tattattggt cgctgaataa agtgctgaat aaaacagcga taccccagat
     4801 aatccatcaa ttgcacctat atcaaccgcc atccagtcat taactcttaa cggttcacct
     4861 ttaactctgt taaagtcaat taattcttct atttctattg caaattgtaa atattccttg
     4921 ttttctgttt ccaggtataa actaattacg aataaaccta tccctgaaag accagagcgt
     4981 aaagaaatat tagtttcatt aatattatct tttaaaaccc tcacttcatt aaagacaact
     5041 tctttataac cttttgcata aagcaaagcc aatattcctg tctttccagt caataatcca
     5101 ttgtcttcaa ttcgagctaa attgtctaat aaaaaacttt gaatccagtt atctacttct
     5161 acagttttag attgttgttt agtaagtgta aaagcggtgc cacttcctcc agtcaaaaag
     5221 ttaaatttcc ccccgctcat catgaactga cgaatgtcac cgttaatgaa cctttcatca
     5281 ttggttaaat tattttcgat tccttttatt aacttataaa taatagacga gatgttaaag
     5341 tcacttgact gatcactaag gttaatttct ttaggcgtat actgttgata atcatttatt
     5401 ctcttattgc atttttcttg taaatctaca atgaaacgat aaaatgaatc tccatagttt
     5461 tcttttatcc aattatgatg gttagattgt agatacccct ctaagtcttc tgacgaaagg
     5521 actggaagca ccaaaaacat tacaattttc tttaatccga accaatctct tgcaccacta
     5581 actttcattt ctcgcgaaac aaatccagtc gtagccatac tagggttgtc ttcagagttt
     5641 acaggcatag cagtttcaaa atctattatc ctaacggtta agtcctcggt taccattaca
     5701 tttgatggtt gcaaatcacc catagctact ccattttgat gcatatgatc tataagcggg
     5761 aaaagttgta acagtatctt tttaacgttt ttagcgtgat cccttataag ttcactgtct
     5821 ttaacgaatg gaaaagttcg ggcaatccat tgccttaagt cttgcccttc tatgaattcc
     5881 tctactaaaa agtaatgttc ccattcttgg aaatactcaa tcaaattaac aacaccgggt
     5941 acatctttaa gtttttttaa ggcatcatat tcaatttttt gcctggataa ggcatcttga
     6001 cctgccccat ctaaaccagc atttggtctg gcctccttga taatcacttt catattatct
     6061 ttctttcgag tcgcacggta taccccacca gcattactat aggtaagtgc agtttcaata
     6121 tcatatttct ctaaattgcc ttccgcttta gtttccaagt cattattaat ggtattcaga
     6181 taatcatcga aatcctttac aaagtcagga acctgataaa aaggagttct ttgatcctta
     6241 attaaatttc cttgctcatc tctaatgcaa tgttcgccat gttcattaat tatagtttta
     6301 aatcctccat atctatagaa tacattgcta ttcttccatc tcttatcatt aagaatataa
     6361 ggtccttttt gaaaatcctg aattgttgaa gaaatcaatt ccaacaattc aacaaagctt
     6421 tcattattaa tagggtatat tgttataaat ttcccagaag aagcacgatt agcatttttc
     6481 gaattcatct caataaaact gtttctatcc ttaaggtgct tgaactcaat ttttctatct
     6541 atacataatc gagcaacctt ttctagcaca ctctgtgaat cttcaaatgt tgcagttaca
     6601 tgaattttcc atccctgttc tggtagtgtt aatcccttga aatgatagtg cttccaaacg
     6661 gagtcactgt ctgaaataac atcatatgta tcaggaatct cattcacttc atatgccttt
     6721 tctatcgcgc tcattttttc gtaatattct gaatccggtt tcatgtactg atgataaagc
     6781 atatttcctt ccatacatat ccccccattt tttattcttt ctctatgtat attccatatg
     6841 tatagggaaa tatcgcctat tttccgcaac atttccttac atattcttca ggtaagatgc
     6901 caataaaagg atttgaaatc gtgatatcgg aaatctcttt ttttgctctt ggaaattttt
     6961 tataatgtaa tactacctag tctgtaataa gggaagcaac atgggtgtgt cctatctcag
     7021 ttattataaa ccctctagca tggtttatta tggaaggtaa atctagtgat tgagacagga
     7081 ttgaatgtga gtattatgat tgactattgg agatgtctgg ctgtcacaaa aaagcttgct
     7141 gattagatat aagcttgtaa tgtgcataca aaaggggata aaatcactta gccacttttc
     7201 tgtacgaatg tagtagccta gaagtatggt gtaagaaaat atagtaatta ttaccattaa
     7261 taataatatt gagtttatag taacatttgg agatgactgt gtcaacgctt tctgattatt
     7321 tttagttatt taatactata tttttatttt ctagtaatag agtactgttc tctctagttt
     7381 tgtattatat aaaaaattta gcagtaatta aatttaatcc ccgtttacaa ttaatgttgt
     7441 tctggatata tcagaaaagt gttgatggag tacttcctca agctcttttt cgattagatt
     7501 ctcaagtgtt ggcaaaattt caaataggtc catcttatta aagcgataac gtgtaaattg
     7561 attggcgata aattgaggtg aattaatggc tcttaagaag ttacccatct ctttcttaat
     7621 tacacgttgt acgtcctctt tctttattga tttcttctta aaaccaacaa tcgtttcatc
     7681 tacaatgttt atgagttttt cgggctcttg gctgtttccg ccaattaccg aaaaaccaaa
     7741 gccctgttca tttgtataat caaaagtaaa tgtctcatct aaataaccat tgttatacat
     7801 cttttcgtac gcttcagagc tattaccaaa cattaattct agtaaaacat ttagagataa
     7861 ctcatattta agcatgtctt gaccttgtct tttagggtta gattctttaa atcctattaa
     7921 cactttaggt gtttcaactg gcatcttcat aatacttgac cttctgttca cttttgcttc
     7981 ttcttcgggg tatattcgat gaatttctgt tggttctcga aatgtttttt ggttctggtt
     8041 gcttcggata agccttatta cttcttcagg aactacaggc ccaataacaa aaagtaacat
     8101 attattagga tgatagaaag tattatagca agtgaataaa tcctctttag tgatttgatt
     8161 gatagaagtt attgttccag ctatatcaat tttcaccgga tgactcttat acatattttc
     8221 taagattcca aatcgagcac gccagtctgg actatcctga tacattttta tttcctgttc
     8281 aatgattcct ttctcctttt ctacgttttc atccgtaaaa tatggttctt gaacaaagtc
     8341 tagaagggtt atcaaattgt tatttatgtt agatgttgct gaaaatgtat acgccgtgcg
     8401 agtaaaactg gtaaatgcat taacaaaagc tccttgtttt gcaaatgtat gaaaaacatc
     8461 aaccttctca ctttcaaaca ttttatgttc taaaaaatgc gctattccat ctggaacttg
     8521 agtggcacaa ttatttcctg gagttgaaaa tttattatct actgaaccat actttgttgt
     8581 gaatgtggca aatgttttat gaaatccttt cttaggaagt atgaaaactt caagaccatt
     8641 ggtgagcttt tcataatata ttgtctcttg tactctttta tagaccattg actccatctt
     8701 atttactcct ttctatttaa aaagtagatt gtatcaagtt cccatttgtt agcagcgcga
     8761 atgacatcgc ttttggatac cctatttatt ctgtttatct gatcaactat gttacttgaa
     8821 aacccgctat caaccgcttc catggatata tctataattc ctaaaggagt atcatatgct
     8881 tcaagtaata ggttaattaa cattgcttta ccttgttcaa cttcatcctc agtgaagtta
     8941 ccttctttca ttgcgtactc ttgttctctt ataatttcaa ctgcacgttc ataattatcg
     9001 aaatcaattc cggccatcgt taataacaac cctttattgc tctctagttg ggaaaataca
     9061 aaataagcaa gacttttctt ttcacgcaca ttaatgaata atttggaaga tggatatctg
     9121 ccaaaaatcg cgtttgccaa ccttgtagct tcaaaatcct catcttgtat tgtcgaataa
     9181 gttctataac ccaaaagaag ctttccttga tttacgtcct gtttatcctg aataatctta
     9241 ataccatttc tatcttttct agctggagaa atggtattta caggtttgtt gttatgctgt
     9301 tcagaaaaaa cgtcatggac cataagttta acctcatcct cattaaaagc accaacgatg
     9361 tataaatcaa accgatcgtt ttgaagcaga tcttcataga tttgataaat ggataaatca
     9421 ctaagggaat caatatcttt ctcgctacca tatgcaggaa gctgatacgg ttcaccttca
     9481 aacatctctt ctatcaaacg aacactagta tataacattt tctcattata cattgaggca
     9541 agtcgttgct ttaaggagcg cttttcttgt tcgacgattg aagaattaaa taccccattt
     9601 tcaagaacgg gactgtaaac aatatcgtgt aataaatcca aagctgattc tagtaacgca
     9661 ttagtcacat taagatattg agctgcaggg atctccattc taaaagatat aatatgattg
     9721 tctccctctt tttttacatc tggggaaaga gttgcaccgt acatctcatc aagcttttgt
     9781 tttattagct tatggtgtgg gtaatttttt gaacctccat tcagaatatg gggaattaat
     9841 gctcttgcag taattaaatc tttttgaaga ggtgacttca tcgtcaacat tatactaatt
     9901 gtcttaaatt tatctgtgtt gcaaagatga aggtgcaacc cgttttgttc actcgaacga
     9961 atagctattt catccat
//
