LOCUS       BGC0002711              9402 bp    DNA     linear   BCT 10-SEP-2019
DEFINITION  Nostoc punctiforme PCC 73102 strain ATCC 29133 chromosome, complete
            genome.
ACCESSION   BGC0002711
VERSION     BGC0002711
KEYWORDS    .
SOURCE      Nostoc punctiforme PCC 73102 (Nostoc punctiforme ATCC 29133)
  ORGANISM  Nostoc punctiforme PCC 73102
            Bacteria; Cyanobacteriota; Cyanophyceae; Nostocales; Nostocaceae;
            Nostoc.
COMMENT     URL -- http://www.jgi.doe.gov
            JGI Project ID: 2351359
            Source DNA available from John C. Meeks (jcMeeks@ucdavis.edu)
            Bacteria available from ATCC: ATCC 29133
            Contacts: John C. Meeks (jcMeeks@ucdavis.edu)
            David Bruce (microbes@cuba.jgi-psf.org)
            Quality assurance done by JGI-Stanford
            Annotation done by JGI-ORNL and JGI-PGF
            Finishing done by JGI-LLNL
            Finished microbial genomes have been curated to close all gaps with
            greater than 98% coverage of at least two independent clones. Each
            base pair has a minimum q (quality) value of 30 and the total error
            rate is less than one per 50000.
            The JGI and collaborators endorse the principles for the
            distribution and use of large scale sequencing data adopted by the
            larger genome sequencing community and urge users of this data to
            follow them. it is our intention to publish the work of this
            project in a timely fashion and we welcome collaborative
            interaction on the project and analysis.
            (http://www.genome.gov/page.cfm?pageID=10506376)
            NOTE: This genome has an insertion element which pops out under
            certain growth conditions. When it pops out, gene Npun_R0414 is put
            together in one piece.
            ##antiSMASH-Data-START##
            Version      :: False
            Run date     :: 2024-10-17 18:15:59
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 2506164
            Orig. end    :: 2515566
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             complement(1..1161)
                     /EC_number="2.3.1.41"
                     /codon_start=1
                     /db_xref="InterPro:IPR013747"
                     /db_xref="InterPro:IPR013751"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) fabH"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1084:3-oxoacyl-(acyl carrier protein) synthase III
                     (Score: 285.5; E-value: 7.8e-87)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="Npun_R2065"
                     /note="PFAM: 3-Oxoacyl-[acyl-carrier-protein (ACP)]
                     synthase III C terminal domain protein;
                     3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III; KEGG:
                     sme:SMc01785 probable 3-oxoacyl-acyl-carrier-protein
                     synthase III"
                     /product="3-Oxoacyl-(acyl-carrier-protein (ACP)) synthase
                     III"
                     /protein_id="ACC80685.1"
                     /sec_met_domain="fabH (E-value: 3.5e-35, bitscore: 110.5,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIYPPVGIRSIAVNFPSVVRTNDYWRKNYPDLVATAEQKSLSKVF
                     SPGDSIPTNEFELKMSPYLKDPFRGTVERRILGPGESSLTLEYGAARDALAAAKLSPEE
                     VDLMIVASLWPENILPGNAAFLAAQLDLRGAAWNLDSTCSNAVVALQNATALVRTGEYR
                     NVLVVVSCTYSRFTDENDTLSWFFGDGAGAFVVGALKQNQGIISTKIVNTCATCDTFYN
                     EFTTDAEGNVRMLIRAGKHTNKVLSETAVNYLRECCLGAIAAAGVTLEQIQFCAFNTPT
                     AWYSSLCTEALGIQQERTINLYPQYANIGPMLPIANLYHGAQLGKIHENDLVLVYSIGS
                     VSNAAATVMRWGNVALSPADKFTTRVPGCLSQEAKKLHSPSITNIG"
     subregion       1..9402
                     /aStool="mibig"
                     /contig_edge="False"
                     /label="PKS"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..9402
                     /contig_edge="False"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            complement(1..1161)
                     /locus_tag="Npun_R2065"
     gene            complement(1342..3102)
                     /locus_tag="Npun_R2066"
     CDS             complement(1342..3102)
                     /EC_number="2.2.1.6"
                     /codon_start=1
                     /db_xref="InterPro:IPR011766"
                     /db_xref="InterPro:IPR012000"
                     /db_xref="InterPro:IPR012001"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TPP_enzyme_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TPP_enzyme_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1055:pyruvate oxidase/decarboxylase (Score: 425.3;
                     E-value: 6.2e-129)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="Npun_R2066"
                     /note="PFAM: thiamine pyrophosphate enzyme domain protein
                     TPP-binding; thiamine pyrophosphate enzyme, central region;
                     thiamine pyrophosphate enzyme TPP binding domain protein;
                     KEGG: ana:all0427 acetolactate synthase large subunit"
                     /product="thiamine pyrophosphate enzyme domain protein
                     TPP-binding"
                     /protein_id="ACC80686.1"
                     /sec_met_domain="TPP_enzyme_N (E-value: 3.6e-37, bitscore:
                     116.5, seeds: 75, tool: rule-based-clusters)"
                     /sec_met_domain="TPP_enzyme_C (E-value: 1.5e-33, bitscore:
                     104.7, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNVETITKTLNSLDQLQRNTAIQSDLFSIEQTSDLTVAAALVKTL
                     ENLGVRYAFGLFGGGITPFIASLEQSSIEVLQFRHEAGAAFAAIESYFASGHPVVVFST
                     TGPGITNALTGLFAARWEGAKVIFVSPCTSATQRGRWAFQETSQYTMSNTGIFTSGTLF
                     HYATTVECNSELSEIARRLALGVGKLGGFVAHISIPRTIQTSLLTTPLPHVNFSPTLVT
                     AGEDTISKCLQLLCEAPFAIWVGFGAKDAALEIRQLAERTGAAVMSSARGKGIFPEDHP
                     QFVGVTGFGGQNSVLKYMQEQRPLRVLVLGTKLGEFTSFWNPVMVPSRGFVHVDIDPEV
                     PGAAYPYAETFSVQSDIGAFVRAILKYFPEISRSSSVLPLPRPEREVIKSHLEGLVRPE
                     FLMDAIQRVIVENSDAIIMTEAGNSFAWGTNILRFAAPGRYRVSTGFGSMGHAGTGILG
                     AALARNRKAVAILGDGAMLMNNEITTAVKHKIPAVWIVLNDARYNMCDQGMNMEGFHNV
                     DAEIPSPDFVMIARGMGADGIRVSRECDLEAALEKALALTIPFVVDVIIDPTRPAPIGS
                     RINSLIFHGK"
     gene            complement(3204..4745)
                     /locus_tag="Npun_R2067"
     CDS             complement(3204..4745)
                     /EC_number="6.2.1.26"
                     /NRPS_PKS="Domain: AMP-binding (13-405). E-value: 1.1e-113.
                     Score: 371.7. Matches aSDomain:
                     nrpspksdomains_Npun_R2067_AMP-binding.1"
                     /codon_start=1
                     /db_xref="InterPro:IPR000873"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002:AMP-dependent synthetase and ligase (Score:
                     422.9; E-value: 2.4e-128)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="Npun_R2067"
                     /note="PFAM: AMP-dependent synthetase and ligase; KEGG:
                     ava:Ava_3987 AMP-dependent synthetase and ligase"
                     /product="AMP-dependent synthetase and ligase"
                     /protein_id="ACC80687.1"
                     /sec_met_domain="AMP-binding (E-value: 6.8e-126, bitscore:
                     409.0, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MMNIAQNLELSCYLFPNKPAIIFEEESFTYKDLDEMVNRVANGLR
                     SLDIRRRDRVALFLPNIPEFVISYLGILKIGAVAVSINIMLKTDEVRYILNDCAAKAII
                     TTESQTEYIQEPDLPQLQHILIAEGRANKGISLKQLMAKSSPEARAVEMSRDDPASILY
                     TSGTTGFPKGATLSHGNVIFNSYCANCCYRIQKSDRLLLFLPLSHCFGQNAILNAGISA
                     CTTIILQRRFDPDQVLETITTHQVTMLFGVPTVFIKLLNMDTSNYNLENIRYYFSAAAP
                     MPLQVAENWYKKFGFVIHEGYGLTETSPFAAYNHNLNYKLGSVGTPIANVEMKIVDGDG
                     NQVEIGELGEITIKGANVMLGYWNRPFETAEVIKNGWLHTGDIGRMDEDGFFYVIDRSK
                     DMIVVSGFKVYPTEVENVIYQHPAVAEVAIYGVPDPLKGEIVKANIILKASQTITEQQI
                     IDFCYERMAAYKVPRVINFVNSIPKNSTGKVLKRFLRQESISQVVEKRQELGVRS"
     CDS_motif       complement(3501..3560)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_Npun_R2067_0003"
                     /evalue="5.70E-03"
                     /label="NRPS-A_a8"
                     /locus_tag="Npun_R2067"
                     /protein_end="415"
                     /protein_start="395"
                     /score="9.6"
                     /tool="antismash"
                     /translation="DRSKDMIVVSGFKVYPTEVE"
     aSDomain        complement(3531..4706)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_Npun_R2067_AMP-binding.1"
                     /evalue="1.10E-113"
                     /label="Npun_R2067_AMP-binding.1"
                     /locus_tag="Npun_R2067"
                     /protein_end="405"
                     /protein_start="13"
                     /score="371.7"
                     /tool="antismash"
                     /translation="LFPNKPAIIFEEESFTYKDLDEMVNRVANGLRSLDIRRRDRVALF
                     LPNIPEFVISYLGILKIGAVAVSINIMLKTDEVRYILNDCAAKAIITTESQTEYIQEPD
                     LPQLQHILIAEGRANKGISLKQLMAKSSPEARAVEMSRDDPASILYTSGTTGFPKGATL
                     SHGNVIFNSYCANCCYRIQKSDRLLLFLPLSHCFGQNAILNAGISACTTIILQRRFDPD
                     QVLETITTHQVTMLFGVPTVFIKLLNMDTSNYNLENIRYYFSAAAPMPLQVAENWYKKF
                     GFVIHEGYGLTETSPFAAYNHNLNYKLGSVGTPIANVEMKIVDGDGNQVEIGELGEITI
                     KGANVMLGYWNRPFETAEVIKNGWLHTGDIGRMDEDGFFYVIDRSKDMIVVS"
     CDS_motif       complement(3633..3713)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_Npun_R2067_0002"
                     /evalue="1.90E-07"
                     /label="NRPS-A_a6"
                     /locus_tag="Npun_R2067"
                     /protein_end="371"
                     /protein_start="344"
                     /score="23.5"
                     /tool="antismash"
                     /translation="IGELGEITIKGANVMLGYWNRPFETAE"
     CDS_motif       complement(4212..4271)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_Npun_R2067_0001"
                     /evalue="1.50E-06"
                     /label="NRPS-A_a3"
                     /locus_tag="Npun_R2067"
                     /protein_end="178"
                     /protein_start="158"
                     /score="20.7"
                     /tool="antismash"
                     /translation="ASILYTSGTTGFPKGATLSH"
     gene            complement(4927..6636)
                     /locus_tag="Npun_R2068"
     CDS             complement(4927..6636)
                     /EC_number="4.3.1.3"
                     /codon_start=1
                     /db_xref="InterPro:IPR001106"
                     /locus_tag="Npun_R2068"
                     /note="PFAM: phenylalanine/histidine ammonia-lyase; KEGG:
                     ava:Ava_3988 phenylalanine/histidine ammonia-lyase"
                     /product="phenylalanine/histidine ammonia-lyase"
                     /protein_id="ACC80688.1"
                     /transl_table=11
                     /translation="MNITSLQQNITRSWQIPFTNSSDSIVTVGDRNLTIDEVVNVARHG
                     TQVRLTDNADVIRGVQASCDYINNAVETAQPIYGVTSGFGGMADVVISREQAAELQTNL
                     IWFLKSGAGNKLSLADVRAAMLLRANSHLYGASGIRLELIQRIETFLNAGVTPHVYEFG
                     SIGASGDLVPLSYITGALIGLDPSFTVDFDGKEMDAVTALSRLGLPKLQLQPKEGLAMM
                     NGTSVMTGIAANCVYDAKVLLALTMGVHALAIQGLYGTNQSFHPFIHQCKPHPGQLWTA
                     DQMFSLLKDSSLVREELDGKHEYRGKDLIQDRYSLRCLAQFIGPIVDGVSEITKQIEVE
                     MNSVTDNPLIDVENQVSYHGGNFLGQYVGVTMDRLRYYIGLLAKHIDVQIALLVSPEFS
                     NGLPPSLVGNSDRKVNMGLKGLQISGNSIMPLLSFYGNSLADRFPTHAEQFNQNINSQG
                     YISANLTRRSVDIFQNYMAIALMFGVQAVDLRTYKMKGHYDARTCLSPNTVQLYTAVCE
                     VVGKPLTSVRPYIWNDNEQCLDEHIARISADIAGGGLIVQAVEHIFSSLKST"
     gene            complement(6747..7748)
                     /locus_tag="Npun_R2069"
     CDS             complement(6747..7748)
                     /EC_number="2.3.1.41"
                     /codon_start=1
                     /db_xref="InterPro:IPR004655"
                     /db_xref="InterPro:IPR013747"
                     /db_xref="InterPro:IPR013751"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) fabH"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1084:3-oxoacyl-(acyl carrier protein) synthase III
                     (Score: 358.8; E-value: 4e-109)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="Npun_R2069"
                     /note="TIGRFAM: 3-oxoacyl-(acyl-carrier-protein) synthase
                     III; PFAM: 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase
                     III C terminal domain protein;
                     3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III; KEGG:
                     ana:alr0239 beta-ketoacyl-acyl carrier protein synthase
                     III"
                     /product="3-oxoacyl-(acyl-carrier-protein) synthase III"
                     /protein_id="ACC80689.1"
                     /sec_met_domain="fabH (E-value: 1.6e-127, bitscore: 413.9,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKSLVRQGIVLTGTGSAVPDQCLDNHQLNQMVVTCDDWIKSRTGI
                     HRRHLLPAKNSLTTMATEAAQKAIDMAGITAQDLDLIILATSTPDDLFGNASQIQARLG
                     ATQAVAFDLTAACSGFVFGLVTAAQFMRTGTYQNVLLIGAEVLSRWVDWSDRRTCILFG
                     DGAGAVVLQASEYDYLLGFQLHTNGTQNQHLKLEFQEEEKVLVDNISVGQGTYKPIAMN
                     GQEVYRFAIQKVPEVIEKALFRAGLDVKQVDWLLMHQANQRILEAVAQRLGIPDEKIIS
                     NLANYGNTSAASIPLALDEAVRRGQIKAHDVVALAGFGAGLSWGAAIFEWGL"
     gene            complement(7879..9147)
                     /locus_tag="Npun_R2070"
     CDS             complement(7879..9147)
                     /EC_number="2.3.1.41"
                     /NRPS_PKS="Domain: PKS_KS(Iterative-KS) (9-413). E-value:
                     8.2e-55. Score: 178.2. Matches aSDomain:
                     nrpspksdomains_Npun_R2070_PKS_KS.1"
                     /codon_start=1
                     /db_xref="InterPro:IPR000794"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) t2fas"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1022:Beta-ketoacyl synthase (Score: 419.7; E-value:
                     2.2e-127)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="Npun_R2070"
                     /note="PFAM: beta-ketoacyl synthase; KEGG: ter:Tery_0451
                     beta-ketoacyl synthase"
                     /product="beta-ketoacyl synthase"
                     /protein_id="ACC80690.1"
                     /sec_met_domain="t2fas (E-value: 9.6e-184, bitscore: 599.7,
                     seeds: 8, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MISKNIRRVVVTGMGAITPLGNTIKEYWEGLIAGRSGIDLITQFD
                     ASLHICRIAGEVRDFNPYDYLERKDVKHISRFAQFGISASLQAIADAGFEINDSNATQV
                     GVIVGNGMGGLYTVEEQEKVLLTQGSKRCSPFTVPMTIANMAAGLIAIYTGAKGPNFCT
                     VSACAAGGNAIGEAFRSIQLGHALGMICGGTEAVITPLSIAAFGSAKTLSTANEEPTRA
                     SRPFDRDRNGFVMGEGAGILFLEELEHALGRGAKIYAEIVGYGLTCDAYHMTAPTPSGE
                     GVMRAMELALKDGGIFPNEVSYINAHGTSTLANDAIETLAIKKALGDRAFQIPISSTKS
                     MTGHLLGAAGGIEAVATVKSVALDLIPPTINLEQPDPNCDLDYVPHKSRTQRVDVALSN
                     SFGFGGHNVSLAFKKFELGVRSY"
     aSDomain        complement(7909..9120)
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_Npun_R2070_PKS_KS.1"
                     /domain_subtypes="Iterative-KS"
                     /evalue="8.20E-55"
                     /label="Npun_R2070_PKS_KS.1"
                     /locus_tag="Npun_R2070"
                     /protein_end="413"
                     /protein_start="9"
                     /score="178.2"
                     /tool="antismash"
                     /translation="VVTGMGAITPLGNTIKEYWEGLIAGRSGIDLITQFDASLHICRIA
                     GEVRDFNPYDYLERKDVKHISRFAQFGISASLQAIADAGFEINDSNATQVGVIVGNGMG
                     GLYTVEEQEKVLLTQGSKRCSPFTVPMTIANMAAGLIAIYTGAKGPNFCTVSACAAGGN
                     AIGEAFRSIQLGHALGMICGGTEAVITPLSIAAFGSAKTLSTANEEPTRASRPFDRDRN
                     GFVMGEGAGILFLEELEHALGRGAKIYAEIVGYGLTCDAYHMTAPTPSGEGVMRAMELA
                     LKDGGIFPNEVSYINAHGTSTLANDAIETLAIKKALGDRAFQIPISSTKSMTGHLLGAA
                     GGIEAVATVKSVALDLIPPTINLEQPDPNCDLDYVPHKSRTQRVDVALSNSFGFGGHNV
                     SLAFK"
     gene            complement(9157..9402)
                     /locus_tag="Npun_R2071"
     CDS             complement(9157..9402)
                     /NRPS_PKS="Domain: PP-binding (8-75). E-value: 5.2e-13.
                     Score: 41.3. Matches aSDomain:
                     nrpspksdomains_Npun_R2071_PP-binding.1"
                     /codon_start=1
                     /db_xref="InterPro:IPR003231"
                     /db_xref="InterPro:IPR006163"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="Npun_R2071"
                     /note="TIGRFAM: acyl carrier protein; PFAM:
                     phosphopantetheine-binding; KEGG: ttj:TTHA0414 acyl carrier
                     protein"
                     /product="acyl carrier protein"
                     /protein_id="ACC80691.1"
                     /sec_met_domain="PP-binding (E-value: 6.5e-14, bitscore:
                     41.3, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTQQDIFNTVQAVVAKELANEQQKITLDANLIQDLGADYLDLIAM
                     FQTLEDTFNTKIPHREAKQLVTVQQIVNYINQKVFI"
     aSDomain        complement(9178..9378)
                     /aSDomain="PP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_Npun_R2071_PP-binding.1"
                     /evalue="5.20E-13"
                     /label="Npun_R2071_PP-binding.1"
                     /locus_tag="Npun_R2071"
                     /protein_end="75"
                     /protein_start="8"
                     /score="41.3"
                     /tool="antismash"
                     /translation="TVQAVVAKELANEQQKITLDANLIQDLGADYLDLIAMFQTLEDTF
                     NTKIPHREAKQLVTVQQIVNYI"
ORIGIN
        1 ctatccaatg tttgtgatgg aaggtgaatg taactttttg gcttcctggc ttaggcatcc
       61 aggtactcta gttgtgaact tatcagctgg acttagagca acattgcccc agcgcattac
      121 agttgcagca gcgttagaca ctgaaccaat agagtataca agaaccaaat cgttttcgtg
      181 aatcttgcct aattgtgcgc cgtggtaaag attagcaatg ggcagcattg gcccaatatt
      241 tgcatattgg ggataaaggt taatcgtccg ctcctgctgg atgcctaatg cttcggtaca
      301 gagggaggaa taccaggcag tgggtgtatt aaaagcacaa aattgaattt gctccagggt
      361 aacaccagcc gcagcgatcg cgcccaagca gcactcacgc aaataattta cagcagtctc
      421 gctcagtact ttgttggtgt gcttgcctgc tcggattaac atccttacat taccctcagc
      481 atccgtagta aattcattat agaaggtgtc gcaagttgca catgtgttca caattttggt
      541 gctaataatt ccctgatttt gtttgagcgc gccaaccaca aaggctccag caccgtcacc
      601 gaaaaaccat gagagagtat cattttcatc agtaaagcga gagtaagtgc aagagactac
      661 caccaacaca ttacgatact ctccagttcg caccagcgca gtagcatttt gcagcgcaac
      721 cacagcatta gaacaggttg aatcaagatt ccaagccgca ccgcgcagat cgagttgagc
      781 agcaaggaaa gccgcattgc caggtagaat attttctggc caaagtgaag caacaatcat
      841 caaatcgacc tcttccggag acagttttgc cgctgccaaa gcgtcccttg ctgcgccata
      901 ctctagcgtg agcgatgact cccccggacc caatattctg cgctcaacag taccacggaa
      961 aggatctttg agatacggtg acatttttaa ttcaaactca ttggtgggga tggaatcacc
     1021 aggcgaaaat acctttgata gacttttttg ttcagctgtt gccactaaat ctgggtaatt
     1081 ctttcgccaa taatcattag tgcgaacaac actaggaaag ttgactgcaa ttgaccgaat
     1141 tcctactggt ggatatatca taataaatct cctgaaaaag tgaggaagtg gaggatgcgg
     1201 ggaagaggca ggggagcagg ggaggattag gggacaagga ggacaaggag gaattattga
     1261 acaagtctct tccttgtctc ccccctcttc tttgtctccc ttgtctcttc tctatgccca
     1321 atgtccaatg cccaattaat attactttcc atgaaaaatg agactgttaa tcctactacc
     1381 aatgggtgct ggacgagtcg gatcaataat cacatcaaca acgaatggaa tagtcaaggc
     1441 tagtgctttt tctaaagccg cttctagatc gcactccctg cttacacgaa taccatcggc
     1501 tcccatacca cgggcaatca tcacgaaatc cgggctggga atctctgcat ccacattgtg
     1561 gaacccctcc atgttcatac cttggtcgca catgttgtaa cgtgcatcat tgagtacaat
     1621 ccagacagca ggaattttgt gtttgacggc ggtagtaatt tcgttgttca tcagcatggc
     1681 accatcaccg agaatggcta cagctttgcg atttcgcgcc agtgcggcac ctaaaatacc
     1741 cgtaccagca tgtcccattg aaccaaatcc agtacttacc cggtaacgcc ctggtgcagc
     1801 aaatcgcagt atatttgttc cccaagcaaa ggagttacca gcctcggtca tgatgattgc
     1861 atcgctattc tcgacaatta ccctttgaat cgcatccatc aaaaattcag gacgtactaa
     1921 accttccaga tgggacttga tgacctcgcg ttcaggacgg ggtaggggta acactgatga
     1981 agaacgagag atttctggaa aatacttcag tatagccctg acaaaagctc cgatatcaga
     2041 ctgaacggag aatgtctctg catacggata agcagctccc ggtacttctg ggtcgatatc
     2101 cacatgaaca aacccccgtg aaggaaccat gactgggttc caaaatgaag taaattcacc
     2161 taacttagtt cccaaaacca gtacgcgcaa aggacgctgt tcctgcatat atttgagaac
     2221 agagttttgc ccgccgaagc cagtcacacc gacaaactga ggatgatcct caggaaagat
     2281 acctttacca cgagctgatg acatcaccgc cgctccggtt ctctcagcaa gttgacgaat
     2341 ttctaaagct gcatctttag caccaaaacc gacccaaatg gcaaacggcg cttcacatag
     2401 cagttgtaaa catttgctga tagtatcttc tccagccgtc accaaagttg gtgaaaaatt
     2461 cacatggggt aatggtgttg ttagcaaact tgtctggatg gttctgggaa tactaatatg
     2521 ggcgacaaag ccacctaatt tccctacacc tagtgccagc ctgcgagcaa tttcagagag
     2581 ttccgagttg cattcaacgg tggtagcata atgaaataat gtgcctgaag taaaaatgcc
     2641 tgtattagac attgtgtatt ggctggtttc ttgaaaagcc cagcgtccgc gctgtgttgc
     2701 agaggtgcaa ggtgatacga aaataacttt tgctccttcc caacgagccg caaacagccc
     2761 agtcaaggca ttagtaatac ctggaccggt tgtgctgaac actacaacag gatgtccgct
     2821 agcaaagtat gactcaatag ctgcgaatgc tgctccggct tcatgacgaa attgcagcac
     2881 ctcgattgag ctttgctcta ggctagctat gaagggtgtg attccccctc cgaacagacc
     2941 aaaagcatag cggactccca aattttccag cgtcttcact aaagctgctg caacagttaa
     3001 atcgcttgtc tgttcaattg agaatagatc cgattggata gcagtgtttc tctgcaactg
     3061 atccaatgaa tttaaggttt tggtaattgt ttctacgttc acgatcgcct actttaattt
     3121 tacaaaaaat aaattaactg gggagtaaaa gggagataaa ataactaact cctgtacaga
     3181 cgcgattaat tgcgtctcta cttctaactc ctaactccta actcctgcct tttttcaaca
     3241 acctgagata ttgattcctg acgtaaaaat cgctttaata ctttaccagt ggagtttttt
     3301 ggaattgagt ttacaaagtt aattacacgg ggaactttat aagctgccat cctttcataa
     3361 caaaaatcaa tgatttgttg ttcggtgatt gtctgactag cttttaggat aatatttgct
     3421 ttgacaatct cgcctttgag aggatcggga acgccatata tagctacttc agctactgct
     3481 gggtgttgat aaatgacatt ttcaacttct gttggatata ccttgaatcc agaaacaacg
     3541 atcatgtctt ttgaacggtc aattacgtag aaaaaaccgt cttcatccat ccgaccaatg
     3601 tcgccagtat gtaaccaacc atttttaatt acttctgcgg tttcaaaagg gcggttccaa
     3661 taaccaagca tgacgttggc acccttaatt gttatctcgc ctaactcacc tatttctacc
     3721 tgattaccat caccatctac aattttcatt tccacatttg caatgggtgt tccaacgcta
     3781 cctagtttgt agttcaagtt gtggttataa gcagcaaatg gcgatgtttc agttaggcca
     3841 tacccttcat ggatgacaaa tccaaacttt ttataccaat tttctgctac ttgcaatggc
     3901 attggtgctg ctgctgaaaa gtaatagcgg atgttttcca ggttatagtt agaagtatcc
     3961 atgttcagta gtttaatgaa tactgtcggc acaccaaata gcattgttac ttgatgtgta
     4021 gtgatggttt ctagaacttg atctgggtca aatcgacgct gtaaaatgat agtcgtacaa
     4081 gcagaaatac cagcatttag aattgcattt tgtccaaaac aatgggataa aggtaagaaa
     4141 agcagtaagc gatcgctctt ttgaatgcga tagcaacaat tagcacaata gctattaaaa
     4201 ataacattgc catgagaaag tgtagcacct ttaggaaagc ctgtagtacc tgatgtataa
     4261 aggatgcttg caggatcatc gcgactcatt tcaacggcgc gtgcttcagg agaagatttt
     4321 gccatcagtt gttttagact aataccttta ttggctctac cttctgcaat caaaatatgt
     4381 tgcaattgag gtaagtctgg ctcttgaata tactcggttt gcgactctgt tgtaataatt
     4441 gctttggcag cacaatcgtt gagaatgtat ctaacttcat cggttttgag cataatgttg
     4501 attgagacag caaccgcgcc aattttcaga atgccaagat aagaaattac aaattctgga
     4561 atgtttggta aaaataaggc tacacgatct ctacgtctaa tatccaaact gcgtagaccg
     4621 ttagcaacgc gatttaccat ttcatcaagg tctttgtaag taaaagattc ttcttcaaaa
     4681 attatggctg gtttgttggg gaaaaggtag caactaagct ctagattttg ggcaatattc
     4741 atcattgaat caccttaata tcttaattcg caatttgtca ttaaaaaatt tttgctttgc
     4801 tgattgagag gatgaattag tctcacgcag aggcgcagag aacgagaaaa gagaaataag
     4861 ggagtagtta cattgaaagg tataaattag taccctaatt cagcaacacc aatttttaat
     4921 tacaaattac gttgacttta agctcgaaaa aatatgctca actgcttgca caattaaacc
     4981 accaccagcg atatcagctg aaatccgggc aatatgctca tctaaacatt gctcgttgtc
     5041 gttccaaatg tatggacgca cagacgttag tggctttcca actacctcgc agactgctgt
     5101 gtataactgc acagtattgg gtgagaggca tgtacgtgca tcataatgac ctttcatctt
     5161 atatgtgcgg aggtcaacag cttgaactcc aaacatcaac gcgatcgcca tataattctg
     5221 aaatatgtct acggaacgac gtgtcaaatt tgcggaaata tagccttggc tgttaatatt
     5281 ttgattaaat tgctcggcgt gggtaggaaa gcgatcggct agggaatttc catagaagct
     5341 caacagtggc ataatcgagt ttccactgat ttgcaaacct ttgagtccca tattaacttt
     5401 gcgatcgcta ttaccaacta aagagggtgg taagccgttg ctaaactctg gcgagacaag
     5461 aagtgcaatc tgcacatcga tgtgtttggc caatagccct atgtaataac gtaggcgatc
     5521 cattgtcaca cccacatact gtccgagaaa attgccgccg tgataactaa cttggttctc
     5581 gacatcaatc aatgggttat cggtgactga gttcatttct acctcgattt gcttggtaat
     5641 ctctgatacc ccatcaacga ttggccctat gaactgtgcc agacagcgga gagaataacg
     5701 atcctgtatc agatctttac cacggtattc gtgtttacca tccaactctt cacgaactaa
     5761 agatgaatct ttcagcagag aaaacatttg atctgctgtc catagttgac cgggatgtgg
     5821 cttgcactga tgaataaacg ggtggaaaga ttgattcgtt ccgtataaac cttggatggc
     5881 taaggcgtgt acacccattg tcagagcgag caaaactttc gcatcgtaca cacagttagc
     5941 tgcaatacct gtcatgactg aggtgccatt catcattgct aaaccttctt tcggttgcaa
     6001 ttgcaacttt ggcaaaccca aacgagacaa ggctgtaacg gcatccattt ctttaccgtc
     6061 gaagtcaact gtaaagctag gatctagacc gattagtgcc ccagtaatgt aggataatgg
     6121 caccaaatcg ccgctagcac cgatagagcc aaactcatag acatggggtg tcacgccagc
     6181 gttgaggaaa gtttcaatcc gctgaataag ttcgagtcgt ataccagacg caccatacaa
     6241 atgtgaattt gcacgtaaga gcatagctgc acgcacgtct gctaacgata atttgtttcc
     6301 tgcgccggat ttcagaaacc aaattaaatt agtctgaagt tccgctgctt gttcgcgaga
     6361 gatgacaaca tctgccatac cgccaaagcc agatgtcacc ccgtaaattg gctgtgctgt
     6421 ttcgactgca ttgttaatgt aatcacaaga tgcttgaaca ccccgaatga catctgcatt
     6481 atcagttaag cgcacctgtg ttccatgacg agcaacattt acaacctcgt cgattgtcag
     6541 attgcgatcg cctacagtta cgattgaatc tgaactatta gtgaaaggta tttgccaaga
     6601 acgcgttatg ttctgttgta gagatgttat attcatacac tggttattct caaattttaa
     6661 caggcctcta tcaagccttt cggaatgtat cttaattacg aattacgtta ggtagcgggg
     6721 cgtagcccat tacgaattac gaattactat agaccccact caaaaatagc agcaccccaa
     6781 gaaagtcctg ctccaaaccc agctaaagca acaacatcgt gcgctttaat ttgccctcgc
     6841 cgaacagctt catccaaagc taggggaatt gaagcagcag aagtatttcc gtaatttgct
     6901 aaattgctaa tgattttttc atcaggaatt cctaaacgtt gagccacagc ctctaaaatc
     6961 cgctgattgg cctgatgcat caatagccag tctacctgtt taacatccag acctgcccgg
     7021 aatagtgcct tttcaataac ttcagggact ttttgaatag caaatcggta aacttcttgt
     7081 ccgttcattg caataggttt atatgtacct tgaccaacgc taatattatc aacaagtact
     7141 ttctcttcct cctggaattc caatttcaaa tgctgatttt gcgtgccatt agtatgaagt
     7201 tgaaaaccaa ggagataatc atattcactt gcttgtaaaa ccactgctcc tgctccatca
     7261 ccaaatagaa tgcaggtacg tcggtcagac caatctaccc accgtgaaag aacttcagca
     7321 ccaatgagaa gaacattttg ataagtacca gtccgcataa attgggcagc agttaccaaa
     7381 ccaaaaacaa aaccagagca agctgctgtt aaatcaaaag ctacagcttg ggtagctccg
     7441 agtcgagctt ggatttgact ggcattacca aacaaatcat caggagtaga tgtagctaag
     7501 ataattaaat ctaagtcttg tgctgttata cctgccatat ctatggcttt ttgagccgct
     7561 tcagtagcca tagttgtgag cgagtttttg gctggaagaa gatggcgacg gtgaattcct
     7621 gtgcgagatt tgatccaatc atcacaagta accaccattt gattgagttg atgattatct
     7681 aagcactgat ctggaaccgc agagccagtc cctgtcaaaa caataccttg tcttactaag
     7741 gatttcatct gctttaattg ctaattattt tgtgtagtta aaatctttga ttctgcttta
     7801 cctttgtaag gtgtgttatg ccgtaggcta acgcaccatc ctagattttc ggtgcgttag
     7861 cttacggcat aacacaccct aataactcct aactcctagc tcaaattttt taaaagctaa
     7921 agagacatta tgtcctccaa aaccaaaaga gttagaaaga gcaacatcta ctctttgagt
     7981 tcgactttta tggggaacat agtctaaatc acagtttgga tctggctgtt ctaaattgat
     8041 tgttggtgga attaaatcaa gagccacact cttgactgta gcaacagctt cgatgcctcc
     8101 agcagcgccc aaaagatggc cagtcattga ttttgtggaa ctaataggaa tttggaaagc
     8161 gcgatcgcca agagcttttt taatcgctaa agtttctatg gcatcattag ctaaggtgct
     8221 agtgccgtga gcattaatat aactgacttc gttggggaat atgccaccat cttttaaagc
     8281 tagttccata gctctcatga ctccctctcc gctaggagtt ggagcagtca tgtggtaagc
     8341 atcacaagtc agaccataac caacaatttc tgcatagatt tttgctccac ggccaagagc
     8401 gtgttctaat tcctctaaga ataaaatgcc tgctccttct cccatcacaa aaccattgcg
     8461 atcacgatcg aacggacgag aggcacgggt gggttcttca tttgcggttg atagcgtctt
     8521 ggcagaaccg aaggcagcta tagacagagg tgtaatcaca gcttctgtac caccacaaat
     8581 cattcctaaa gcatgaccta actgaattga gcgaaaagct tctccgatag cattacctcc
     8641 agcagcgcaa gctgaaacag tacaaaagtt tggcccttta gctcctgtgt aaattgctat
     8701 tagtccagct gccatgttag ctattgtcat cggcactgta aaaggactac agcgtttaga
     8761 tccctgagtc aggagaactt tttcttgctc ctctacagta tagaggccgc ccataccgtt
     8821 gcccacaata acgcccactt gagtagcatt agagtcatta atttcaaaac cagcatcggc
     8881 gatcgcttga agacttgcag agattccaaa ttgagcaaaa cgagatatat gcttaacatc
     8941 tttgcgttct agataatcat aagggttaaa gtctctaact tcgcctgcaa ttctacatat
     9001 atgcaaagaa gcatcaaatt gggtaatcaa atcaattcca cttcgtcccg caatcaatcc
     9061 ttcccaatat tccttgatgg tatttcctaa aggggtaata gctcccatac ctgtaactac
     9121 aacccgccgg atatttttgc taatcataag taaaactcaa ataaaaactt tctgattgat
     9181 gtaattaacg atttgttgta ctgttacaag ttgctttgct tcccgatgag gaatttttgt
     9241 attgaaagta tcctctagtg tttggaacat tgctatcaag tctaaataat ctgcccctaa
     9301 atcttgaata agattagcat ctagggtgat tttttgctgt tcatttgcta attctttggc
     9361 aacaacagct tggactgtgt taaatatatc ttgttgggtc at
//
