BGC0002735 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0002735 (Streptomyces incarnatus)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	NRPS,T1PKS	1	90,408	High	incarnatapeptin A/incarnatapeptin B/dentigerumycin F/dentigerumycin G	PKS+NRPS:Type I

BGC0002735 - Region 1 - NRPS,T1PKS

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Location: 1 - 90,408 nt. (total: 90,408 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 90,409
Label		BGC0002735

Tailoring functions

Oxidoreductases: dehydrogenase oxygenase

ABB07_39165: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

ABB07_39200: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

ABB07_39210: 3-hydroxybutyryl-CoA dehydrogenase

smcogs: SMCOG1095: 3-hydroxybutyryl-CoA dehydrogenase

ABB07_39220: monooxygenase FAD-binding

smcogs: SMCOG1050: monooxygenase FAD-binding

Transferases: acylase

ABB07_39175: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

ABB07_39180: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

Hydrolases: esterase

ABB07_39145: thioesterase

smcogs: SMCOG1004: thioesterase

ABB07_39170: thioesterase

smcogs: SMCOG1004: thioesterase

ABB07_39215: thioesterase

smcogs: SMCOG1004: thioesterase

Ligases: ligase synthase

ABB07_39125: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

ABB07_39130: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

ABB07_39135: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

ABB07_39140: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

ABB07_39185: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

ABB07_39190: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

ABB07_39195: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

ABB07_39205: 3-oxoacyl-(acyl carrier protein) synthase III

smcogs: SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002735.2	1.92	NRPS, PKS	incarnatapeptin A, incarnatapeptin B, dentigerumycin F, dentigerumycin G	Streptomyces incarnatus
BGC0001273.3	1.40	PKS	asperlactone	Aspergillus ochraceus
BGC0001244.3	1.39	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001275.3	1.38	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0002075.3	1.15	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0001132.4	1.14	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001825.4	1.12	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001128.4	1.11	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002135.2	1.10	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0002286.3	1.09	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002735.2	0.99	NRPS, PKS	incarnatapeptin A, incarnatapeptin B, dentigerumycin F, dentigerumycin G	Streptomyces incarnatus
BGC0001273.3	0.76	PKS	asperlactone	Aspergillus ochraceus
BGC0001244.3	0.76	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001275.3	0.76	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001833.4	0.75	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0002135.2	0.75	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001128.4	0.74	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002075.3	0.74	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0001132.4	0.73	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002286.3	0.73	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 90408:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (D-X - Ala) + (D-Leu - Leu) + (Thr) + (Ala) + (pk) + (ccemal) + (redmal) + (Me-mal) + (X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 0 - 63268:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (D-X - Ala) + (D-Leu - Leu) + (Thr) + (Ala) + (pk) + (ccemal) + (redmal)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 28640 - 87036:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (D-Leu - Leu) + (Thr) + (Ala) + (pk) + (ccemal) + (redmal) + (Me-mal) + (X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 4, location 47276 - 90408:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk) + (ccemal) + (redmal) + (Me-mal) + (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ABB07_39125: X - Ala

Search NORINE for peptide: strict or relaxed

AMP-binding (466..863): X

nrpys: Piz

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVFSVASYAK
Nearest Stachelhaus code(s):: Piz DVFSVASYAK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2017..2396): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: DVPDEALVEK
Nearest Stachelhaus code(s):: Ala DVPDEALVEK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

ABB07_39130: Leu - Leu

Search NORINE for peptide: strict or relaxed

AMP-binding (465..852): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: ala (Ala)

Stachelhaus prediction details:

Stachelhaus sequence:: DVFSVAAYAK
Nearest Stachelhaus code(s):: Leu DVFSVAAYAK (85% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1993..2381): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVFSVAAYAK
Nearest Stachelhaus code(s):: Leu DVFSVAAYAK (94% 8Å match)
Stachelhaus code match:: 100% (strong)

ABB07_39135: Thr

Search NORINE for peptide: strict or relaxed

AMP-binding (466..876): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: DFWNVGMVHK
Nearest Stachelhaus code(s):: Thr DFWNVGMVHK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

ABB07_39140: Ala

Search NORINE for peptide: strict or relaxed

AMP-binding (466..842): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: gly (Gly)

Stachelhaus prediction details:

Stachelhaus sequence:: DMTQIGWTWK
Nearest Stachelhaus code(s):: Ala DMTQIGWTWK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

ABB07_39175: pk

PKS_AT (525..822): pk

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 91.7%; Methoxymalonyl-CoA: 83.3%; Malonyl-CoA: 83.3%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 194.0
Methylmalonyl-CoA: 192.4
Methoxymalonyl-CoA: 154.8
2-Methylbutyryl-CoA: 128.5
trans-1,2-CPDA: 104.5
Malonyl-CoA: 104.5
CHC-CoA: 103.0
Propionyl-CoA: 89.9
Isobutyryl-CoA: 63.9
Benzoyl-CoA: 53.2
Acetyl-CoA: 46.4
inactive: 38.0
3-Methylbutyryl-CoA: 32.4
fatty_acid: 19.6

ABB07_39180: emal

PKS_AT (587..886): emal

ATSignature: Ethylmalonyl-CoA

Top 3 matches:: Ethylmalonyl-CoA: 87.5%; Methylmalonyl-CoA: 79.2%; Methoxymalonyl-CoA: 79.2%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 175.4
Methylmalonyl-CoA: 170.3
Methoxymalonyl-CoA: 148.4
2-Methylbutyryl-CoA: 112.1
trans-1,2-CPDA: 103.2
Malonyl-CoA: 99.7
Propionyl-CoA: 95.9
CHC-CoA: 85.7
Isobutyryl-CoA: 67.4
Benzoyl-CoA: 52.4
inactive: 50.3
Acetyl-CoA: 42.2
fatty_acid: 26.1
3-Methylbutyryl-CoA: 20.3

ABB07_39185: mal

PKS_AT (568..866): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 83.3%; Methylmalonyl-CoA: 66.7%; CE-Malonyl-CoA: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 149.1
Methylmalonyl-CoA: 74.6
Methoxymalonyl-CoA: 65.1
inactive: 50.2
Propionyl-CoA: 46.0
Ethylmalonyl-CoA: 42.0
fatty_acid: 35.4
CHC-CoA: 30.8
2-Methylbutyryl-CoA: 28.6
Isobutyryl-CoA: 23.2
Acetyl-CoA: 23.1
trans-1,2-CPDA: 19.4
Benzoyl-CoA: 18.6
3-Methylbutyryl-CoA: 0.0

ABB07_39190: mmal

PKS_AT (575..871): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 215.2
Ethylmalonyl-CoA: 212.3
Methoxymalonyl-CoA: 180.9
2-Methylbutyryl-CoA: 166.0
trans-1,2-CPDA: 132.4
Propionyl-CoA: 119.1
Malonyl-CoA: 115.9
Isobutyryl-CoA: 100.6
CHC-CoA: 100.4
Benzoyl-CoA: 71.9
Acetyl-CoA: 49.0
inactive: 47.8
3-Methylbutyryl-CoA: 46.3
fatty_acid: 35.3

ABB07_39195: X

AMP-binding (451..848): X

nrpys: Ile, Val, aIle

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: DALWWGGVFK
Nearest Stachelhaus code(s):: Ile DALWWGGVFK (65% 8Å match); Val or aIle DALWWGGVFK (65% 8Å match)
Stachelhaus code match:: 100% (strong)

Link to PARAS substrate predictor