BGC0002806: oximidine I biosynthetic gene cluster from Pseudomonas baetica a390
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Location: 606,241 - 702,336 nt. (total: 96,096 nt).
This entry is originally from NCBI GenBank PKLC01000016.

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core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
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Gene details
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General
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NRPS/PKS domains
General information about the BGC

MIBiG accession | BGC0002806
Short description | oximidine I biosynthetic gene cluster from Pseudomonas baetica a390
Status | Quality: questionable The quality level of this entry. Status: pending The status of this entry. Completeness: complete Whether the entry covers everything needed for the pathway producing the compound(s)

Biosynthetic class(es)

- NRPS (Type I)
- PKS (Type I)

Loci
PKLC01000016
613752 - 688705
via Knock-out studies [2], Enzymatic assays [2]
Compounds

- oximidine I
- 17Z-oximidine III
- oximidine IV
- oximidine V
- deoxy-oximidine

Species Pseudomonas baetica a390 [taxonomy]
References

- [1] Polyketide Synthase‐Mediated O‐Methyloxime Formation in the Biosynthesis of the Oximidine Anticancer Agents. Vriens, E et al., Angewandte Chemie International Edition (2023) DOI:10.1002/anie.202304476
- [2] Polyketide Synthase-Mediated O-Methyloxime Formation in the Biosynthesis of the Oximidine Anticancer Agents. Vriens E et al., Angew Chem Int Ed Engl (2023) PMID:37218580

Chemical products information
oximidine I Evidence: NMR [2]
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C23H24N2O7
Chemical database entries
NPAtlas
17Z-oximidine III Evidence: NMR [2]
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C23H24N2O6
oximidine IV Evidence: NMR [2]
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C23H24N2O7
oximidine V Evidence: NMR [2]
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C23H24N2O6
deoxy-oximidine Evidence: NMR [2]
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C23H24N2O5
List of genes involved in compound(s) production

Identifiers | Position | Product | Functions | Evidence | Extra
--- | --- | --- | --- | --- | ---
C0J26_09355 PTC20173.1 | 612930 - 614015 (+) | restriction endonuclease |  |  | copy AA seq copy Nt seq
C0J26_09360 PTC20174.1 | 614147 - 614917 (+) | trans-aconitate 2-methyltransferase |  |  | copy AA seq copy Nt seq
C0J26_09365 PTC20175.1 | 614956 - 615684 (-) | class I SAM-dependent methyltransferase |  |  | copy AA seq copy Nt seq
C0J26_09370 PTC20176.1 | 616255 - 616617 (+) | hypothetical protein |  |  | copy AA seq copy Nt seq
C0J26_09375 PTC20177.1 | 616689 - 617948 (-) | type II toxin-antitoxin system HipA family toxin |  |  | copy AA seq copy Nt seq
C0J26_09380 PTC20178.1 | 617941 - 618258 (-) | XRE family transcriptional regulator |  |  | copy AA seq copy Nt seq
C0J26_09385 PTC20179.1 | 618442 - 618849 (-) | hypothetical protein |  |  | copy AA seq copy Nt seq
C0J26_09390 PTC20448.1 | 618885 - 619073 (-) | hypothetical protein |  |  | copy AA seq copy Nt seq
C0J26_09395 PTC20180.1 | 619026 - 620251 (+) | hypothetical protein |  |  | copy AA seq copy Nt seq
C0J26_09400 PTC20181.1 | 620223 - 621191 (-) | hypothetical protein |  |  | copy AA seq copy Nt seq
C0J26_09405 | 621224 - 621707 (+) | hypothetical protein |  |  | copy AA seq copy Nt seq
C0J26_09410 | 621741 - 622394 (-) | lipase |  |  | copy AA seq copy Nt seq
C0J26_09415 PTC20182.1 | 622474 - 623505 (-) | hypothetical protein |  |  | copy AA seq copy Nt seq
C0J26_09420 | 623637 - 624627 (-) | perosamine synthase |  |  | copy AA seq copy Nt seq
C0J26_09425 | 624621 - 625010 (-) | hypothetical protein |  |  | copy AA seq copy Nt seq
C0J26_09430 PTC20183.1 fabD | 626241 - 628121 (+) | [acyl-carrier-protein] S-malonyltransferase |  |  | copy AA seq copy Nt seq
C0J26_09435 PTC20184.1 | 628125 - 633476 (+) | hypothetical protein |  |  | copy AA seq copy Nt seq
C0J26_09440 PTC20185.1 | 633476 - 633892 (+) | hypothetical protein |  |  | copy AA seq copy Nt seq
C0J26_09445 PTC20186.1 | 633874 - 637404 (+) | hypothetical protein |  |  | copy AA seq copy Nt seq
C0J26_09450 PTC20187.1 | 637424 - 645742 (+) | hypothetical protein |  |  | copy AA seq copy Nt seq
C0J26_09455 PTC20188.1 | 645739 - 662025 (+) | hypothetical protein |  |  | copy AA seq copy Nt seq
C0J26_09460 PTC20189.1 | 661827 - 663869 (+) | hypothetical protein |  |  | copy AA seq copy Nt seq
C0J26_09465 PTC20190.1 | 663853 - 669759 (+) | hypothetical protein |  |  | copy AA seq copy Nt seq
C0J26_09470 PTC20191.1 | 669528 - 670895 (+) | hypothetical protein |  |  | copy AA seq copy Nt seq
C0J26_09475 PTC20192.1 | 670895 - 682336 (+) | hypothetical protein |  |  | copy AA seq copy Nt seq
C0J26_09480 PTC20193.1 | 682387 - 683808 (+) | hypothetical protein |  |  | copy AA seq copy Nt seq
C0J26_09485 PTC20194.1 | 683863 - 684618 (+) | hypothetical protein |  |  | copy AA seq copy Nt seq
C0J26_09490 PTC20195.1 | 684615 - 685418 (+) | 4-phosphopantetheinyl transferase |  |  | copy AA seq copy Nt seq
C0J26_09495 | 685450 - 686980 (+) | hypothetical protein |  |  | copy AA seq copy Nt seq
C0J26_09500 | 687104 - 687478 (-) | pyocin |  |  | copy AA seq copy Nt seq
C0J26_09505 | 687734 - 687892 (-) | hypothetical protein |  |  | copy AA seq copy Nt seq
C0J26_09510 PTC20196.1 | 688080 - 688730 (-) | hypothetical protein |  |  | copy AA seq copy Nt seq

Biosynthesis information

#### Biosynthetic paths

(E)-2-(hydroxyimino)acetic acid
Show structure
Pathway: [oxiA]+oxiB [1]
Comment: module 1
(E)-2-(methoxyimino)acetic acid
Show structure
Pathway: oxiC+oxiD [1]
Comment: module 1
Annotation changelog

#### Entry version: 1

Date
Changes
Submitters
Reviewers
2024-07-25
MIBiG v4 annotathon

- (ID: BYMOQMBRAB25QU6RBYAHVZZ5)
- (ID: 7LZK2REXWMB23Y766VYXAX3J)
- (ID: 3UOU7PODQJXIM6BEPUHHRRA5)

- (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

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