BGC0002808: N-coronafacoyl-L-isoleucine biosynthetic gene cluster from Streptomyces scabiei 87.22
Shows the layout of the region, marking coding sequences and areas of interest. Clicking a gene will select it and show any relevant details. Clicking an area feature (e.g. a candidate cluster) will select all coding sequences within that area. Double clicking an area feature will zoom to that area. Multiple genes and area features can be selected by clicking them while holding the Ctrl key.
View antiSMASH-generated output
Download Cluster GenBank file
Download region SVG
Location: 8,793,133 - 8,848,674 nt. (total: 55,542 nt).
This entry is originally from NCBI GenBank FN554889.1.

#### Legend:

core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
TTA codons
reset view
zoom to selection
Gene details
Shows details of the most recently selected gene, including names, products, location, and other annotations.
Select a gene to view the details available for it
General
Compounds
Genes
Biosynthesis
History
KnownClusterBlast
NRPS/PKS domains
General information about the BGC

MIBiG accession | BGC0002808
Short description | N-coronafacoyl-L-isoleucine biosynthetic gene cluster from Streptomyces scabiei 87.22
Status | Quality: questionable The quality level of this entry. Status: active The status of this entry. Completeness: complete Whether the entry covers everything needed for the pathway producing the compound(s)

Biosynthetic class(es)

- PKS (Type I)

Loci
FN554889.1
8805124 - 8836499
via Gene expression correlated with compound production, Knock-out studies
Compounds

- N-coronafacoyl-L-isoleucine

Species Streptomyces scabiei 87.22 [taxonomy]
References

- [1] Positive and Negative Regulation of the Virulence-Associated Coronafacoyl Phytotoxin in the Potato Common Scab Pathogen Streptomyces scabies. Cheng, Z et al., Molecular Plant-Microbe Interactions® (2019) DOI:10.1094/MPMI-03-19-0070-R
- [2] Characterization of the Coronatine-Like Phytotoxins Produced by the Common Scab Pathogen Streptomyces scabies. Fyans, J et al., Molecular Plant-Microbe Interactions® (2015) DOI:10.1094/MPMI-09-14-0255-R
- [3] Streptomyces scabies 87-22 Contains a Coronafacic Acid-Like Biosynthetic Cluster That Contributes to Plant–Microbe Interactions. Bignell, D et al., Molecular Plant-Microbe Interactions® (2010) DOI:10.1094/MPMI-23-2-0161
- [4] Production of the Streptomyces scabies coronafacoyl phytotoxins involves a novel biosynthetic pathway with an F420‐dependent oxidoreductase and a short‐chain dehydrogenase/reductase. Bown, L et al., Molecular Microbiology (2016) DOI:10.1111/mmi.13378
- [5] Coronafacoyl Phytotoxin Biosynthesis and Evolution in the Common Scab Pathogen Streptomyces scabiei. Bown, L et al., Applied and Environmental Microbiology (2017) DOI:10.1128/AEM.01169-17
- [6] Regulation of Coronafacoyl Phytotoxin Production by the PAS-LuxR Family Regulator CfaR in the Common Scab Pathogen Streptomyces scabies. Cheng, Z et al., PLOS ONE (2015) DOI:10.1371/journal.pone.0122450
- [7] Purification of N-coronafacoyl Phytotoxins from Streptomyces scabies. Bown, L et al., BIO-PROTOCOL (2017) DOI:10.21769/BioProtoc.2214

Chemical products information
N-coronafacoyl-L-isoleucine Evidence: MS/MS
Copy SMILES
Chemical database entries
PubCHEM
List of genes involved in compound(s) production

Identifiers | Position | Product | Functions | Evidence | Extra
--- | --- | --- | --- | --- | ---
SCAB_79581 CBG74918.1 | 8805124 - 8807034 (-) | conserved hypothetical protein |  |  | copy AA seq copy Nt seq
SCAB_79591 CBG74919.1 | 8807131 - 8807928 (-) | conserved hypothetical protein |  |  | copy AA seq copy Nt seq
SCAB_79601 CBG74920.1 | 8809277 - 8809552 (+) | putative acyl carrier protein |  |  | copy AA seq copy Nt seq
SCAB_79611 CBG74921.1 | 8809549 - 8810046 (+) | putative polyketide dehydratase component |  |  | copy AA seq copy Nt seq
SCAB_79621 CBG74922.1 | 8810043 - 8811185 (+) | putative polyketide KS component |  |  | copy AA seq copy Nt seq
SCAB_79631 CBG74923.1 | 8811182 - 8811709 (+) | putative polyketide synthase component |  |  | copy AA seq copy Nt seq
SCAB_79641 CBG74924.1 | 8811711 - 8813120 (+) | putative polyketide synthase ligase component |  |  | copy AA seq copy Nt seq
SCAB_79651 CBG74925.1 | 8813133 - 8821295 (+) | putative polyketide synthase |  |  | copy AA seq copy Nt seq
SCAB_79661 CBG74926.1 | 8821292 - 8828674 (+) | putative polyketide synthase |  |  | copy AA seq copy Nt seq
SCAB_79671 CBG74927.1 | 8828821 - 8830368 (+) | putative coA ligase |  |  | copy AA seq copy Nt seq
SCAB_79681 CBG74928.1 | 8830365 - 8831195 (+) | conserved hypothetical protein |  |  | copy AA seq copy Nt seq
SCAB_79691 CBG74929.1 | 8831332 - 8832528 (+) | putative monooxygenase |  |  | copy AA seq copy Nt seq
SCAB_79701 CBG74930.1 | 8832525 - 8833940 (+) | putative oxidoreductase |  |  | copy AA seq copy Nt seq
SCAB_79711 CBG74931.1 | 8833955 - 8835640 (+) | putative oxidoreductase |  |  | copy AA seq copy Nt seq
SCAB_79721 CBG74932.1 | 8835717 - 8836499 (+) | putative dehydrogenase/reductase |  |  | copy AA seq copy Nt seq

Biosynthesis information

No biosynthesis information available for this record.

Annotation changelog

#### Entry version: 1

Date
Changes
Submitters
Reviewers
2023-11-19
Submitted

- (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

- (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

2023-11-19
Reviewed and approved

- (ID: DKBFG3K6XTYSVEQEQVCFZBWY)

- (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

2023-11-20
Reviewed and approved

- (ID: C62OJVVH7XOTOTFZSJT727YU)

- (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

Similar known clusters from MIBiG 4.0
Shows clusters from the MIBiG database that are similar to the current region. Genes marked with the same colour are interrelated. White genes have no relationship.
Detailed help and explanations are available here.
Click on reference genes to show details of similarities to genes within the current region.
Double click on a reference drawing to reverse the display of the genes.
Click on an accession to open that entry in the MIBiG database.
Location:
Identifier
Identity
Coverage
Detailed domain annotation
Shows NRPS- and PKS-related domains for each feature that contains them. Click on each domain for more information about the domain's location, consensus monomer prediction, and other details.
A domain glossary is available here, and an explanation of the visualisation is available here.
Selected features only
Show module domains