BGC0002808 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0002808 (Streptomyces scabiei 87.22)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS	1	55,542	High	N-coronafacoyl-L-isoleucine	PKS:Type I

BGC0002808 - Region 1 - T1PKS

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Location: 1 - 55,542 nt. (total: 55,542 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 55,543
Label		BGC0002808

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase oxygenase

SCAB_79681: oxidoreductase

smcogs: SMCOG1083: oxidoreductase

SCAB_79691: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

SCAB_79701: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

SCAB_79711: 3-hydroxybutyryl-CoA dehydrogenase

smcogs: SMCOG1095: 3-hydroxybutyryl-CoA dehydrogenase

SCAB_79721: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Ligases: ligase synthase

SCAB_79621: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

SCAB_79641: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

SCAB_79651: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

SCAB_79661: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

SCAB_79671: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002808.2	1.00	PKS	N-coronafacoyl-L-isoleucine	Streptomyces scabiei 87.22
BGC0000041.3	0.69	PKS	oronofacic acid	Pseudomonas syringae
BGC0001276.3	0.67	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0002154.2	0.67	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001257.3	0.67	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0001275.3	0.66	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.66	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001284.4	0.66	PKS	alternariol	Parastagonospora nodorum SN15
BGC0002191.2	0.66	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0002192.3	0.64	PKS	FR901512	fungal sp. No.14919

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002808.2	1.00	PKS	N-coronafacoyl-L-isoleucine	Streptomyces scabiei 87.22
BGC0000041.3	0.69	PKS	oronofacic acid	Pseudomonas syringae
BGC0001276.3	0.67	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0002154.2	0.67	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001257.3	0.67	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0001275.3	0.66	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.66	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001284.4	0.66	PKS	alternariol	Parastagonospora nodorum SN15
BGC0002191.2	0.66	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0002192.3	0.64	PKS	FR901512	fungal sp. No.14919

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 55542:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (pk - redemal) + (redmal)
Non-colinear ordering:: SCAB_79651 + SCAB_79661

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

SCAB_79641: X

AMP-binding (39..382): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: -AASTGTRVK
Nearest Stachelhaus code(s):: Gln or Tyr DAESVGTVVK (47% 8Å match)
Stachelhaus code match:: 60% (weak)

SCAB_79651: pk - emal

PKS_AT (93..387): pk

ATSignature: Ethylmalonyl-CoA

Top 3 matches:: Ethylmalonyl-CoA: 66.7%; Methylmalonyl-CoA: 62.5%; Methoxymalonyl-CoA: 58.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 119.2
Ethylmalonyl-CoA: 117.0
Methoxymalonyl-CoA: 103.6
Propionyl-CoA: 82.2
2-Methylbutyryl-CoA: 79.7
trans-1,2-CPDA: 74.8
CHC-CoA: 65.8
Benzoyl-CoA: 41.0
Isobutyryl-CoA: 38.3
Malonyl-CoA: 36.2
3-Methylbutyryl-CoA: 27.2
Acetyl-CoA: 16.4
fatty_acid: 10.8
inactive: 9.2

PKS_AT (1107..1404): emal

ATSignature: Ethylmalonyl-CoA, Methoxymalonyl-CoA

Top 3 matches:: Ethylmalonyl-CoA: 83.3%; Methoxymalonyl-CoA: 83.3%; Methylmalonyl-CoA: 79.2%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 184.9
Methylmalonyl-CoA: 171.7
Methoxymalonyl-CoA: 143.4
2-Methylbutyryl-CoA: 114.2
trans-1,2-CPDA: 102.5
Propionyl-CoA: 93.1
Malonyl-CoA: 78.9
CHC-CoA: 71.6
Isobutyryl-CoA: 53.9
Benzoyl-CoA: 34.7
Acetyl-CoA: 30.9
3-Methylbutyryl-CoA: 28.3
inactive: 25.6
fatty_acid: 6.4

SCAB_79661: mal

PKS_AT (593..898): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 75.0%; Methylmalonyl-CoA: 66.7%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 199.5
Methoxymalonyl-CoA: 87.0
Methylmalonyl-CoA: 81.3
inactive: 69.1
Propionyl-CoA: 50.0
Ethylmalonyl-CoA: 42.5
Isobutyryl-CoA: 38.8
Benzoyl-CoA: 22.5
CHC-CoA: 21.2
trans-1,2-CPDA: 19.6
Acetyl-CoA: 16.5
2-Methylbutyryl-CoA: 12.7
fatty_acid: 8.8
3-Methylbutyryl-CoA: 0.0

SCAB_79671: X

AMP-binding (6..404): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: PWLGVGVAPK
Nearest Stachelhaus code(s):: OH-Asn PAWCFGVAPK (26% 8Å match)
Stachelhaus code match:: 60% (weak)

Link to PARAS substrate predictor