BGC0002815 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0002815 (Acanthopleuribacter pedis)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	NRPS,NRPS-like,T1PKS	1	63,685	acidobactamide A/acidobactamide B/acidobactamide C	NRPS:Type I+PKS:Modular type I	100%

BGC0002815 - Region 1 - NRPS,NRPS-like,T1PKS

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Location: 1 - 63,685 nt. (total: 63,685 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))
NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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Gene details

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Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 63,686
Label		BGC0002815

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002518	1.04	NRP	syringafactin A, syringafactin C	Pseudomonas sp. SZ57
BGC0001133	1.04	NRP	taxlllaid A	Xenorhabdus bovienii SS-2004
BGC0002135	1.03	NRP	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0002437	1.03	NRP	thermoactinoamide A	Thermoactinomyces sp. AS95
BGC0001844	0.94	NRP	holrhizin	Paraburkholderia rhizoxinica HKI 454
BGC0002071	0.94	NRP	virginiafactin A, virginiafactin B, virginiafactin C, virginiafactin D	Pseudomonas sp. QS1027
BGC0001135	0.93	NRP	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001833	0.92	NRP	icosalide A, icosalide B	Burkholderia gladioli
BGC0000396	0.91	NRP	nodularin	Nostoc sp. 73.1
BGC0000426	0.90	NRP	sevadicin	Paenibacillus larvae

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001133	0.68	NRP	taxlllaid A	Xenorhabdus bovienii SS-2004
BGC0002518	0.68	NRP	syringafactin A, syringafactin C	Pseudomonas sp. SZ57
BGC0002437	0.68	NRP	thermoactinoamide A	Thermoactinomyces sp. AS95
BGC0002135	0.67	NRP	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001844	0.66	NRP	holrhizin	Paraburkholderia rhizoxinica HKI 454
BGC0002071	0.66	NRP	virginiafactin A, virginiafactin B, virginiafactin C, virginiafactin D	Pseudomonas sp. QS1027
BGC0001135	0.65	NRP	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001758	0.65	NRP	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001833	0.64	NRP	icosalide A, icosalide B	Burkholderia gladioli
BGC0001825	0.64	NRP	xenematide	Xenorhabdus nematophila AN6/1

Similar known gene clusters from MIBiG 4.0

Module view

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 63685:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X) + (ohmal) + (mal - D-Thr) + (X - Thr - Me-ohmal) + (Gly) + (X) + (D-Ile - Asn)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 0 - 28959:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - Thr - Me-ohmal) + (Gly) + (X) + (D-Ile - Asn)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

J3U88_09280

Search NORINE for peptide: strict or relaxed

AMP-binding (423..710): Ile

nrpys: Ile

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: ile (Ile)

Stachelhaus prediction details:

Stachelhaus sequence:: DAFFLGVTFK
Nearest Stachelhaus code(s):: Ile DAFFLGVTFK (79% 8Å match)
Stachelhaus code match:: 100% (strong)

J3U88_09290

Search NORINE for peptide: strict or relaxed

AMP-binding (499..893): Ile

nrpys: Ile

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: ile (Ile)

Stachelhaus prediction details:

Stachelhaus sequence:: DALFMGVTFK
Nearest Stachelhaus code(s):: Leu or Ile DALFMGATFK (76% 8Å match)
Stachelhaus code match:: 90% (moderate)

AMP-binding (1998..2384): Asn

nrpys: Asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asn (Asn)

Stachelhaus prediction details:

Stachelhaus sequence:: DLSKIGEVGK
Nearest Stachelhaus code(s):: Asn DLTKIGEVGK (82% 8Å match)
Stachelhaus code match:: 90% (moderate)

J3U88_09295

AMP-binding (501..857): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: SALELQALVK
Nearest Stachelhaus code(s):: Leu DALLLGAVVK (35% 8Å match); Trp DSLELVAVVK (32% 8Å match)
Stachelhaus code match:: 60% (weak)

J3U88_09300

Search NORINE for peptide: strict or relaxed

AMP-binding (528..932): Gly

nrpys: Gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: gly (Gly)

Stachelhaus prediction details:

Stachelhaus sequence:: DILQLGLIWK
Nearest Stachelhaus code(s):: Gly DILQLGLIWK (91% 8Å match)
Stachelhaus code match:: 100% (strong)

J3U88_09305

Search NORINE for peptide: strict or relaxed

AMP-binding (1007..1401): X

nrpys: Val, Leu, Ile, Abu, Ival

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DPRHFGSQIK
Nearest Stachelhaus code(s):: D-Asp DPRHVGIFIK (50% 8Å match)
Stachelhaus code match:: 70% (weak)

AMP-binding (2049..2453): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: DFWNIGMVHK
Nearest Stachelhaus code(s):: Thr DFWNIGMVHK (94% 8Å match)
Stachelhaus code match:: 100% (strong)

PKS_AT (3169..3447): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 83.3%; inactive: 79.2%; 2-Methylbutyryl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 147.6
inactive: 75.6
Methoxymalonyl-CoA: 64.4
Methylmalonyl-CoA: 44.7
Propionyl-CoA: 40.9
2-Methylbutyryl-CoA: 24.3
CHC-CoA: 21.9
Ethylmalonyl-CoA: 16.4
fatty_acid: 16.3
Benzoyl-CoA: 13.9
trans-1,2-CPDA: 11.8
Isobutyryl-CoA: 10.6
Acetyl-CoA: 9.3
3-Methylbutyryl-CoA: 0.0

J3U88_09310

Search NORINE for peptide: strict or relaxed

PKS_AT (527..815): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 83.3%; inactive: 70.8%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 149.8
inactive: 67.8
Methoxymalonyl-CoA: 48.9
Methylmalonyl-CoA: 46.9
Ethylmalonyl-CoA: 31.4
Propionyl-CoA: 29.1
2-Methylbutyryl-CoA: 27.5
fatty_acid: 24.0
CHC-CoA: 21.4
Isobutyryl-CoA: 20.6
Benzoyl-CoA: 16.4
Acetyl-CoA: 14.8
trans-1,2-CPDA: 14.0
3-Methylbutyryl-CoA: 0.0

AMP-binding (1981..2390): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: DFWSVGMVHK
Nearest Stachelhaus code(s):: Thr DFWSVGMVHK (94% 8Å match)
Stachelhaus code match:: 100% (strong)

J3U88_09315

PKS_AT (531..813): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 83.3%; inactive: 70.8%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 146.9
inactive: 78.3
Methoxymalonyl-CoA: 78.1
Methylmalonyl-CoA: 62.6
Isobutyryl-CoA: 47.4
Propionyl-CoA: 41.7
Ethylmalonyl-CoA: 32.3
Benzoyl-CoA: 30.0
Acetyl-CoA: 28.8
3-Methylbutyryl-CoA: 26.7
2-Methylbutyryl-CoA: 22.7
fatty_acid: 19.2
CHC-CoA: 18.6
trans-1,2-CPDA: 11.3

J3U88_09320

CAL_domain (227..642): pk

minowa: fatty_acid

Prediction, score:

fatty_acid: 16.1
Acetyl-CoA: 14.1
shikimic_acid: 0.0
NH2: 0.0
AHBA: 0.0

AMP-binding (1826..2216): X

nrpys: Tyr, Pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Polar aromatic ring (Tyr, bOH-Tyr)
Single AA prediction:: tyr (Tyr)

Stachelhaus prediction details:

Stachelhaus sequence:: DASTIAAVCK
Nearest Stachelhaus code(s):: Tyr or Pro DASTIAAVCK (82% 8Å match); Tyr DASTIAAVCK (76% 8Å match)
Stachelhaus code match:: 100% (strong)