LOCUS       BGC0002855             18333 bp    DNA     linear   PLN 29-APR-2023
DEFINITION  Aspergillus striatus prenyltransferase (estC1), terpene cyclase
            (estB1), FAD-dependent monooxygenase (estM1), and geranylgeranyl
            pyrophosphate synthase (estG1) genes, complete cds.
ACCESSION   BGC0002855
VERSION     BGC0002855
KEYWORDS    .
SOURCE      Aspergillus striatus
  ORGANISM  Aspergillus striatus
            Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina;
            Eurotiomycetes; Eurotiomycetidae; Eurotiales; Aspergillaceae;
            Aspergillus; Aspergillus subgen. Nidulantes.
REFERENCE   1  (bases 1 to 18333)
  AUTHORS   Bundela,R., McLellan,R.M., Cameron,R.C., Nicholson,M.J. and
            Parker,E.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2022) Ferrier Research Institute, Victoria
            University of Wellington, Kelburn Parade, Victoria University of
            Wellington, Wellington 6012, New Zealand
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: ABySS v. v. 16/11/2016
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
            ##antiSMASH-Data-START##
            Version               :: 8.dev-5707d64f
            Run date              :: 2025-08-15 22:38:06
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..18333
                     /culture_collection="ATCC:64988"
                     /db_xref="taxon:469280"
                     /mol_type="genomic DNA"
                     /organism="Aspergillus striatus"
                     /strain="ATCC 64988"
     region          1..18333
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="terpene"
                     /region_number="1"
                     /rules="(PT_phytoene_like or phytoene_synt or Lycopene_cycl
                     or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA
                     or TS_Pyr4)"
                     /subregion_numbers="1"
                     /tool="antismash"
     subregion       1..18333
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0002855"
                     /subregion_number="1"
                     /tool="antismash"
     cand_cluster    182..18333
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /kind="single"
                     /product="terpene"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    182..18333
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="True"
                     /core_location="[10181:17320]"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10182..17320
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
     gene            complement(<10182..>11298)
                     /gene="estC1"
     CDS             complement(join(10182..10500,10554..10906,10954..11298))
                     /codon_start=1
                     /gene="estC1"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: PT_FPPS_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1182: Polyprenyl synthetase"
                     /gene_kind="biosynthetic"
                     /note="EstC1"
                     /product="prenyltransferase"
                     /protein_id="WGJ63575.1"
                     /sec_met_domain="PT_FPPS_like (E-value: 4.2e-61, bitscore:
                     194.6, seeds: 145, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQGEHSFSLAASSTSTGMFIASLILMFGLGAVAVDRLPLRGWFSQ
                     RSRMIGPKQPAKTTGLECPYEYIRKAYGHHHWAPFIDKLSPTLREGNPAKYYHINEVMD
                     GIHLCLILVDDISDASVCRKGHPAAHILYGPSETLNRAYYVVTQLLTKTTLDFPHLAPW
                     LLQDLEDILHGQDLSLNWRRDGLSSLPADPAERAESYKQMASLKTGSLFRLLGHLALED
                     RTMDGLLTQVAWYSQLQNDCKDVFSSEYGKLKGSAAEDLSNKEFTYPIMLALGAPGGHN
                     VAQALESPTPRNLRRALRVIQSNEVYGRCMHELKQASVGIEDWLRLWGRKEKLDGGR"
     gene            <11666..>12439
                     /gene="estB1"
     CDS             join(11666..12183,12232..12439)
                     /codon_start=1
                     /gene="estB1"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: TS_Pyr4"
                     /gene_kind="biosynthetic"
                     /note="EstB1"
                     /product="terpene cyclase"
                     /protein_id="WGJ63576.1"
                     /sec_met_domain="TS_Pyr4 (E-value: 1.2e-114, bitscore:
                     369.7, seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDGYDVSQAPPEYHAVKPLVDSLILGMGLGWMINYLGMVYQSFRE
                     ETYGMAIIPLCCNIAWEIVYAVIYPSKDSHERGVFFGGLIINLAIIYAAIRFSPNEWAH
                     APLVRDNLRWIFLVGILVFLTGHLALVAEMGFSLAYLWSAAFCQVVLSLGGLCQLLCRN
                     RTRGASYLLWGSRTFGTFSGVVALFIRWKYWPESFEWLNSPLMLWCLTVSLLFDGSYGV
                     CFWYVKRSEEMLLAQRKER"
     gap             12671..12790
                     /estimated_length=120
     gene            <12955..>14507
                     /gene="estM1"
     CDS             join(12955..13247,13311..13956,14013..14507)
                     /codon_start=1
                     /gene="estM1"
                     /gene_functions="other (smcogs) SMCOG1087: hypothetical
                     protein"
                     /gene_kind="other"
                     /note="EstM1"
                     /product="FAD-dependent monooxygenase"
                     /protein_id="WGJ63577.1"
                     /transl_table=11
                     /translation="MGEQTFKVIIVGGSIAGLTLAHCLERIGIDYLVLEKASEIAPQVG
                     ASIGVFPNGARILDQLGIYGDLEEVIEPMGQATVCYPDGFSFSSSYPQIIHKRFQYPIA
                     FLDRQKLLEILYTKLPDRSKIRLRSRVASITTLRDNGGVNVTTDDAQTYTGHLVVGADG
                     VHSRVRSEMWKAAQSDGRGPTAKEQQGMTTEYCCIFGISSAVKGLNVGEQVNAFYDHLT
                     IMTVHGRKGRIYWFFIQKLDQRYIYPDNPNYGPEHATLVAERFRHRRLCNDITFGHVWD
                     NREIATITPLEEGLFQTWYHGRIVLMGDSVHKMTPNLGQGANMAIEDAASLASLLNNLL
                     DGGDGQRLTREGIEAMLRQYRDSRYSRVRAVYNTSRFLVRFQARNGLFNTLFGRYYAPY
                     AGDLPADIGSKTIAGAEVCSFLPIPSSADAWDRYRSRGWRESIGAKEVFGLVIIIGLAL
                     QYFGPIGCPGAFCASSRLT"
     gene            <16013..>17320
                     /gene="estG1"
     CDS             join(16013..16195,16346..16500,16553..16879,16933..17320)
                     /codon_start=1
                     /gene="estG1"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: PT_FPPS_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1182: Polyprenyl synthetase"
                     /gene_kind="biosynthetic"
                     /note="EstG1"
                     /product="geranylgeranyl pyrophosphate synthase"
                     /protein_id="WGJ63578.1"
                     /sec_met_domain="PT_FPPS_like (E-value: 3.5e-106, bitscore:
                     342.6, seeds: 145, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGSTVSLPQSVSALWERLSFPFRVDYATKSPSSTPCNEINSPITK
                     NEPTEVFGPPRICKKPIIRRPLDYLLSLPGKQLRTQLVLAFNEWFRVPDDKLQLIVDAT
                     ELLHTASLLVDDIQDGSKLRRGHPAAHTVFGSAQTINSANFAYFLAQERLGELRSIDAF
                     QIFITELLNLHRGQGLELYFRDCLICPTEEEYLEIVTHKTGGLFRLAVGLLQSQSEISL
                     DCRALADTLGVIYQIRDDYLNLQSDQYAKSKGFGEDLTEGKFSFPIVHSILSAPENTTL
                     IDILGQRTEDEAVKLSAIRHMESTGSFEYCRQRLNTLQTEAISMIEQIEDIQGPGHGIR
                     RILRCLMLTS"
ORIGIN
        1 ttgggcgtaa taagtcttaa cccctttcct cgcatatata ttagcaggca ggtagcaggg
       61 ctagccaggg cagaagagag cgcgtcgtcc cttcctgctc cccgtagttt cccaactacg
      121 tccggacctt ggcctacccc tgggcctgct atctagacgc gctttgatac gcctgtttag
      181 catggtgccg catatttgca cctcgtaatt tacagcaaaa ggatgtgatc cctgatatat
      241 gaagtcaacc tgagttgata tcccagcgca ggtagtactc cgccatgacc gaagccagtc
      301 gctgaagcca tctgggacgg gacatcccga caaagcttcg gaaggggatc ttgatagagt
      361 caggacccga ctgactctca atgagccatg ccattctctg agtaccaggt atcctggttt
      421 gattatctac ctacccagcg tgacaacata ctcccctccc tcttccgtct ccagaccctc
      481 ctcgtatttc tccccattaa caaagaactc cgccccgtcc tgtactcgaa ctctcattgt
      541 acccccacca agtgacctta taactgccgt ccgcaacctc ccgtcctccc aatctacgtc
      601 aaccgcaacc cctcctctcg ccattaaacc cctcgccgat cccctcggca cagcaaccgc
      661 aggcaatgcc ggcaagagat gcacaacacc cgcgtgactc tgcagcagca tctcggtgat
      721 cccacttacg accccaaaat ttccatcaat ctggaatgtc gccccatcat ccgtattcca
      781 gagagtatcc gtcggataga ggctaaacca ctttccgatc tgcgcccagg catcgtctcc
      841 ccggtatagc cgtgcatact ggttgataaa ccatgcgtta ctccaccctg tgcttccaga
      901 gccgtggcta aggcgatcgt caaggaggac ttctgccgcg gcgctgagag tgctattcac
      961 tagtggcgag aattggcctc cagggtgcag accgaagagg ggggagagat ggcggtggcc
     1021 aggggcggac gattcatagt cgagtcgcca ttcctggatt tggccttggg atccgatacg
     1081 tggtggatgg atcttgtgga ggaaggactt tgcggctgaa actgtatggt agtcgtcggc
     1141 gattccaagt tctgacgccg cgtcgagcag gttgtgcagg acttcccaga taatctggtc
     1201 gtccatggga atagcaatgt ccattgccgc tttgttcccg gcaatggtcc agttctctgg
     1261 aattatgaag gtattctctg gggagaggga gggcccggtc actttgtacc cttcgtgctc
     1321 gaaggtatag cactggtaga actttgcaac gtcgacaaga tacgggtaga tcgtatcagc
     1381 caggagtacc ttatcaccgg taaaccggta ctgatcgtat aagtgcgttg caagccaagc
     1441 agcacccatc ggccacatac ttgacgaggt atagttgtca acgggggctg ggtcgcccca
     1501 tacatcaagg ttatggtgga atacgacgcc gctgcagttg tacatatctc ttgcaagctt
     1561 ctggccgcgc gggtatgcga cagcgaacag atcgaatagc ggctcctgtg tctcaatgag
     1621 attagttgga ccggcgggcc agtagttcat ctccgtgttg atgttgatcg tgtactttcc
     1681 gccccaggca gccgttgtct ggttattcca tatcccctgc aggtttgccg gcaaatcaat
     1741 agcttcgctg gtgtttcgtg atgacgcgac gagcaggtgc cggccgtagt tccatgccaa
     1801 cgtggcgagt tccgggtccc cgtctagttc tccagctgag cggacgagtg cgattcgctc
     1861 gtccgtggct aggtcgcggg tttcgtcggt tgagttgccg agatcgattg aggcacgctc
     1921 gaggagggat gatgagtcgg caagtgcctc gtccctgatc ttctcgtagc gtttgttcag
     1981 ggcgtcggtg agtttgcggg cgatctctgc gtcgattgcg ttctggctag ggtagcggta
     2041 gttggtttcg gcgtcgaaga ggatgtcgac tgttgtggcg ttgatgatag tgagagtgcc
     2101 gttgctggag gtgaagtggg ctcaaggaag tcagctggca tacgctcgca cgagatgaaa
     2161 gacgtagcat acccccttca gccacaaaac tcgcctgccc actaaacaca attgggtttt
     2221 cctcagcaga ctgtccgctc gtcccactca acctgatcca cggtgccgta tccgacgcag
     2281 aagcctgcaa cgacgtgacg tccgacacac gagaaaacgt cgcattcagc gtcagcgcac
     2341 ccttctcact ggcagtgaac cgcgcagcaa gaaccccggc cgggtagctc gcgatatact
     2401 cccgtctgtg tcaatactat cgtcagctct cccatgcagg aggggcgcga gggcctgccc
     2461 tgcacgactc acgtatactt cactccttcc accacatact caaccccaac atcccccttt
     2521 cgcgtgtcca accacctcct atatccttct actttcgcct cgtcatgtcc aaatcccagt
     2581 tccagattcc caaaataact atacgcccgc atgctatcga tctcatgcat catttccccc
     2641 tggataaact cccccgcttc agtgatgctc ccgttcagca gcagtttcct tgcaatggtc
     2701 aatgcagcag gagcgttctc tggcgtccgt tcctggaacg ggccggacca gatggtgttc
     2761 tcgtttaatg tgacgatttc ggcgcgtacg cctccataga tcgtcgctgc aaggcgccca
     2821 tttccgattg gcaatgcgcc gttttcccag tctgttgccg gggcagggga tgtgtaccag
     2881 aggtggcggg atgccgaaag gtccggggag gcgagggttg gtcgtgaaga gagaagaagg
     2941 ggtgagagga gcggtatgtg atgtagtttc attgtgggca gccgcgctga ccggtagcta
     3001 tactgtgtat aatctgagaa cctgacaatg gttatgctat taaattcaat gccgtggaga
     3061 aaatgctttg aagatgtgtg ttggcgagct tgatatatat ttatatacat atttcgccaa
     3121 cgtcgggcat aactcaaaac gtcgtcctcc tcccgccgct gtaggtcatc gcgtcgggta
     3181 ataggataat aacaaggaac tgcatgtatc actcctcgtc ttgaactctg cacgtacctg
     3241 ttctacctgg atgctgcggg gaaggttgag ttagggctaa agcaattggt ggacaaaccc
     3301 ggggtatgca gatcatgtcc agggtgggca gaacatgaat aacgcgagtg atactgatat
     3361 cgaagtgaat tctaaaacgg ctaaattgcc taggagtcgt aaaagtctcc gtttctggat
     3421 gaaggctctg ccagcgccaa ccacaaagcg catcaaaaga gttctgaggg atatggtcaa
     3481 ctgtcacgag gccgaaacat cataaacagg gtttgtcccc ttggtgatat attggttagg
     3541 cacggctgtt gaacaatagt ctatccaggg ctgagcgttg gctggtcgcg ggcagccggc
     3601 cgcggtcgac agccgcacct tttatgatcg acctaatcgc ttgatggact gaagccttga
     3661 gcagtaactc tgaaatttca ctgatcgctt tgtgactata acaaacgcat gctagataaa
     3721 tcgagatact tgagcgtgat agctcaaaga gcagtaagta catcacgtct gaccatgcgg
     3781 agttcaaagg atacattcgg tcagaggggt tgtggagtac ttatagacgt ctgaccggcc
     3841 aagaagaaaa tcaagagcgg tataatggca gtatcattaa gtacatcagg tcctcttgta
     3901 tttagctggc taccgtgttg gactgcgata gaataaaaga gcaatccata tgtcgccagt
     3961 tcctgacaca agtactatgg aggtatgatg atagacttgg agctaacaca atattaggtc
     4021 tcctgagact tagtcatgtt tgtgccagag taaagcgagc tatggataat agctatagaa
     4081 taggcaggga agcagcacgg ctcaatacaa atatctcaaa agtaaagaaa tagtgtttta
     4141 acatataaat agtaaatggg aaatttagaa taacaatccc cttgtggtgt agttggttta
     4201 tcacgtctga ctgtaatgga acaaacatta aatcagaagg tcaccggctc gactccggtc
     4261 gaggggatca atattttttt ttctgcacta cgatccgtgc tttttaattg tgtggctgaa
     4321 ataaatgtgg gtatgtggag atatgaggcg gtgggtttgg cgcggaccgt aagaagagta
     4381 gagcgcatgt tactggctta ctggcttact cgctttatac ttgaaggata cggaccatta
     4441 aaataaaaga ccagcgaaca ctgagtttgt tgtggaatat gctcaaaggg gaaagggcag
     4501 gaggaaactg tatcaactac accaacgtag cctactaagg atcggatgcc atatgacgat
     4561 tgaccatcta tcttgttgac gatagattcc ctattgcttt tttcttactc ttctgttccc
     4621 ccgctatata tacatacaga gggtgggaac cttctagaac taggcgatat ccggtcaatt
     4681 caccggagat cacgtggcct catatttccg tatcactccc cttcgttcaa taccccgaaa
     4741 cgaacggcat gaagctatac aagcttccaa acaggaaatc aaacaaaaca agtggagggg
     4801 tgttgaacgc ctttatctta tcaatctgcg gatcttgatg ttgtatcata gaaccttatt
     4861 ttgactccag ctgcagcann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnca tacttaatac ctgtttagtc atatattagt ggtcatgtat gttgatgaag
     4981 acatcttagt aaagatgggt tgggaactgt tatcaaaata tatgtttcta accctgtacc
     5041 aaactaacaa ataactacnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt
     5101 tggtatgcct aggtattttg tatagtatat tttctatata cgagcatgtt tcatctgttt
     5161 tcatagtcat tgatgctgaa ttaccacgca atgtgtaaga acggcccaaa acagtgttta
     5221 ataacagtaa ataacgagat gaatcatcaa tttatgcagc cgaaaaggga aggtcttggc
     5281 acaattatga aattgcggtt caccatggtg aagaccgcaa gggaccgcgg tctcgacctg
     5341 aggaccaaga ccgcaccatg cagaggccta ctcccagcat attctcagga cggtgttatc
     5401 ctttcccgtg tctatcaagg ctcaacgaat ctttcttctt tgaggacttc attgaagagt
     5461 tactccatta tagcggaaaa tggccagagc caaaatcagt ccttgttatg gacagtgctt
     5521 cctttcacca ttctgagaag ataaagcaaa tgtgttcaca gacagggggt caagctagta
     5581 tacttgcctc cttactcacc gggtttaaac acaattgagg agctcttcgc tgagttaagc
     5641 tgctattcac cagaataatg tcggggatta gatttggatt tgcttttggc gatttttgac
     5701 gatatagaag ggttctgaaa ggcgcgtcac ggagtataac tctgtaacga ctagaccgat
     5761 gcatatcaga gaacaggtag agaccgaaaa gaatggtcca cttacaaact gcccgatata
     5821 tgtaccgtcc tgtctctccc ataaatcagc catagtccga taagctcgag ctagttcaac
     5881 tatccgagtg ggaggaagga aatcctatga tgaagaccca ccaatctacc tctattactt
     5941 tattgggtga aaagttactg caaacaattg agtggtggca aaatatacta acctagacct
     6001 ggtgtattag cgcctagtac ttactgggtt aaggttttga gtaaaagctt gagaatcggg
     6061 taaaaacaaa aactcagcat agcagatcgg aagacacagc agtagtaatg tcagtaaatg
     6121 atcactccca acatgatcta aataagtagt ttgacaagac tagtattgaa tgatcaactg
     6181 tggagcagca gcctgttaca caaaagcaag aagctcacac taggtatctc atttaaccat
     6241 gtggatgatg aattttcgac tgcgtcaggc aacaacaaga ggggaacaaa aatctatgac
     6301 ccgtataagt ctgtagacat gatcccaaat tggagcttgc cctgtagtac tttcagcact
     6361 catatgagca tcctggtcag caagtatttt cagttaccat ggaaggccat gcaacttggg
     6421 caaatagtgt cctccgccat acagctttga aaacctcatt gagttatcat tatacttcag
     6481 gcaggggttc cttaggagtc aaaacccaaa gcattcttgt ttgccctagc gaatgttcgg
     6541 tagtatgttt caacaaattt tcctcattgc taaataccca cagagtgact actgtgtttt
     6601 taggatacct tgtaatgcag gtgttcctgc gcccagaggg aaagcggact ggcctccatg
     6661 gcggtgcgga atattttgat tgcacagtcc ttgaacaaca gctgcagaca aaaccgcgct
     6721 atgtggctta agaatcatgt ccttaccctc gacgtataag ccatttgtcg gtgaacgact
     6781 cccttcaatt agccgagagt aatttaagtg gccacgttgc tgtcagttag gagtatcgag
     6841 atccctgttc cgcagcatct agatcaagcc aagcgccggg ggaataccct gaagggttca
     6901 agggtgatta gtatatggct tggagaacct ccgcggggcc ctaattgctt gatgaaacag
     6961 ctaaggggta acggctcgaa cctatgcatt catgcaaatt cttcagccga atttgaggcc
     7021 taggcctaag tgctccatgt ttcaaccggt atttaaggac ggcttacctc gactgctgtc
     7081 cttggtctcc aggcccgcag cgccaaaagt tcacgatgaa gcatacagtt ttgactgccg
     7141 cactggcctt gggcttcagc tgcgctacaa gcgctggcgg cgtccagtcg tttgggacca
     7201 ttatcgatcg agcggcggac ctgtttcctg tatacgacta tgttgttgtc ggtggtggtg
     7261 tcagcggcct tgtggttgca aaccgtctga ccgaagatgt caatagtatg tcagtccatc
     7321 ggtttatgaa aggttcttct gctccattga tgacgctact gacagtttta cagcgaccgt
     7381 cctggtaatt gaagctggca agatgtagat tctgtactac cttagaccga actcctcatg
     7441 ctaactgctc agcgacaact actccaaagt catccagtac cctcattacc tctctcttgc
     7501 ttcgacaaac cacagctggc ccatcacctc tcttcctgtt gccggactga acaaccgcac
     7561 agctgctgtg ccttcagctc gaattctggg tggagggagc gccattaatg gtatggcatt
     7621 tgaccgtgga tctccaggcg attacaacct ctggggcgag attatcggtg acagcacctg
     7681 gagctgggag ggcctcctgc cgtacttcag aaaggtgcgt ctctcgatgg agactaaaac
     7741 tagaagtatg ctgacagata tactcagagc gaaacattta cacccccgac tgaagcacaa
     7801 cagaaggagt tcggcatctc cttcgacatg gcggtccacg gaacgaccgg tccgctacag
     7861 tcgtcgtacc ctccattcat ttcaaagacc agcagtatgt ctaacggcgc tactccgtgc
     7921 tcattactga catattaaac agaggccttt atcaacgccc tccgccagct gcgaatccca
     7981 atccaacgtg acggtacggc caacgcgatc ggtggattct ggaaccccaa ttctctcgac
     8041 ccggtcacca gagagcgatc gtacgcacga acgacctttc acgaagaggc taagagccga
     8101 ctaaactacc atgtgctgcc ccagaccctc gtcaccaagc tcacatcgga tctatccgct
     8161 gtcgagtaca ttactggcta caaccctgat caaacaaccg caccttccaa cgcacaagtt
     8221 tccagagtcg gggcgaggaa ggagataatc atggctgctg gaggtgtcca cacgcccaaa
     8281 atccttgagc tatccggtat cggctcatca agcgttctga agtcccttgg tgtgaaacaa
     8341 atcatcaatc tccccggtgt tggtgagaac ctgcaggacc acccagtgct ctacggcggc
     8401 gccactacta atgtgaccga cgcttcaact acgatggctg tgaacgctac atttgaggca
     8461 gagatgggtg tcctctacga acggaacagg acaggtccct ggacggtatc tacagggaac
     8521 acgttcgctt tcttgaccgc ggaacatctg aatgtaactg cagcgaccct tgctcaggcg
     8581 gccgaccaat ctgttggaga gtatctccgg gttggtcttg atcgctcggt agtcgaggga
     8641 tacgaaaaga tcaaggctct gactctcaac gccatggcta agaacaaagt agccctgacc
     8701 gagaacctct ttggcggcac cgcctgtctc cagaagccgt tgagcagggg ttcaatccac
     8761 gcgggctcaa ataaccccta cgacatgccc aatgtcgact tccgctcgtt gagtaacccg
     8821 cttgaccttc aggtcttgat ccagtccatc cggttcaata cggacgtcct cccaacaacg
     8881 caagcataca aagaaatcga ccttgccgtc acatacccgc aacccggttc atctgatgcc
     8941 gagctcacga aggtggtgag gagcgctgcg ttcccgagct tcgcgcatcc gagtgggacg
     9001 tgcgcgatgt tgaacctaga ggatggtggg tgcgtggata gtcagttgag actttatgga
     9061 tccaagggta gaatcagggt tgtcgatgcg agtatcttcc cagtgattcc gtcgacgcat
     9121 acgcagagca cggtgtatgc ggttgcggag aaggcagcgg atattatcaa gggagcttag
     9181 actgcacatt gaaaacccta gttctaactc tctacgacaa agcgagcatt agactcgagc
     9241 acagtctttc actctgttac ctttaacaga ttcacttctc cgccgtctgc aggtgcatgc
     9301 ccatctgagc aattcacgtc tcctcgacat cccacacaac gcccttccac tcccaaaagc
     9361 caacctcata cttgtgtggc ccattttccg aatcctattt caaatcttct ctagcagcat
     9421 ccacagttgt cacctccctc tcaaaacctc tcccagtaat actctccaac aattctgcca
     9481 attcagcata aatgaccgta tttctagcat taaaccacct cccaacatcc caaacactcg
     9541 caccatcctc ttcgccccaa caacctcctc cgccgctctc ctaaaatccc ccggactctt
     9601 gagcgtaacc cgaactcccc acccactcag cgccctaaca attccttaca aattcacaac
     9661 cccgaaactc ttctcaaaca gagtatctaa gctgctctgt gaattcactt tcttcatttc
     9721 tatctcatag ctcatgagaa tacagaggca ataaaagact atatagctag taagggtagt
     9781 ttttgtctgc tactgttgaa agcatatatc aggttccttt gtatgcgata tggaaatgtc
     9841 atgtataagc gatgtactcg ctggccggct tacgccacgt gacatcgtgg cttagacgcg
     9901 atctccaacc cctgaggaaa tatgccccaa ttggggccgc cagagcgctt gatagcgcaa
     9961 cagtccatac ttctactacc tggataaagg gcccggcccc cccgacacaa tacccggagt
    10021 gcgcgcggaa aatcaaacgt gatctcaaaa gcatgtttga taagataagg ctctatggtc
    10081 gccgtgtacc cttcactgtt ccaggctaat tcatcctatg gaggcaacac cctctttctc
    10141 cccgtagccc agacgccaca agagaccatc tcatatgcaa ttcagcgccc cccgtcaagt
    10201 ttttctttcc ggccccatag ccgaagccag tcctcgattc ccacgctcgc ctgcttgagc
    10261 tcatgcatgc atcggccata aacctcgttg ctctgaatga cgcgcaaagc tcgtcgcaaa
    10321 ttacgaggag ttggcgattc cagggcctgg gcaacgttgt gaccccctgg cgccccaagg
    10381 gccagcataa tcgggtaggt aaactctttg ttgctaaggt cttcagctgc agagcctttg
    10441 agcttgccgt attccgaaga aaagacgtcc ttgcagtcat tctgcagttg ggaataccag
    10501 ctgccgagtt cattaatgat tgtccacgcg ataggtgcga aggtttcacg tacgcaactt
    10561 gggttaggag cccatccatt gttcgatctt ccaaagccaa gtgtccaaga agtcggaaga
    10621 gagaccccgt cttcaacgat gccatctgtt tatatgactc ggcacgttcg gcagggtcag
    10681 cgggcaggct actcagacca tcacggcgcc agttgagcga cagatcctga ccatgaagga
    10741 tgtcctctaa gtcctgaagc aaccatgggg ccaaatgggg aaaatcgagt gttgttttcg
    10801 tgaggagttg tgtcactaca tagtaagcgc gattgagggt ctctgatgga ccgtataata
    10861 tatgtgcagc cggatgacct ttgcgacaga cgctcgcatc cgaaatctag agacttcgat
    10921 cagtcaaagc tcgcagcaac agacgtcacg tacatcgtcc accaaaatta gacagaggtg
    10981 aatgccgtcc atgacctcat tgatgtggta gtatttggca ggatttcctt ctcggagagt
    11041 cggggagagt ttgtcgataa agggtgccca gtggtgatga ccgtaggcct tgcgtatata
    11101 ctcgtacgga cattcaagtc ccgttgtttt ggcgggttgc ttaggtccta tcattcgaga
    11161 ccgctgagaa aaccatccac gaaggggtag tcgatcaaca gcgactgcac caagaccaaa
    11221 catgagaata agagatgcga tgaacatgcc cgtcgaggtt gagctcgccg ccagactgaa
    11281 gctgtgctca ccctgcattt tttttgtcgg cttgatgaaa attcaatata cgaatgccag
    11341 acgttcgcgg ctcgcagctt cttacaaccc aggggttaga ttttatgcct gcttaagtgg
    11401 ttcaggccac tacacagaac aggccagttg cttccacgtc acatcacgct gcaggtccta
    11461 gcgtgcatct gcacctcgaa gcacaccgga ctccgagggc gtctcaataa atactagctc
    11521 tcttcagggc actgacgctg tgccacacga attgaatata aataggcggg aagagtattg
    11581 tgcctacgat cctcgtatcg ccgcaacgcc ttattatccg cgtcgtcgcc tatcttttct
    11641 cgtccggctg aaagacactc ccgagatgga cggctacgat gtttctcagg cacctccaga
    11701 gtatcatgcg gtgaagccgc ttgtcgactc gcttatcctt gggatgggcc tcgggtggat
    11761 gatcaactac ctgggcatgg tgtatcagtc cttccgggaa gaaacttacg ggatggccat
    11821 cataccgctg tgttgtaata ttgcctggga gattgtttac gcggttatct acccttcaaa
    11881 ggacagccat gagcgaggtg tcttctttgg gggcttaatc atcaacctgg ccatcatcta
    11941 tgccgccatc cgcttttccc ccaacgagtg ggcacacgcc cccctggtaa gagacaacct
    12001 ccggtggatc ttcctcgtcg ggattcttgt cttcctcacg ggccatttgg ccctggtggc
    12061 tgagatggga ttctcccttg cctacttgtg gagtgcggca ttctgccagg tggttctcag
    12121 tctcggggga ctctgccagc tgttatgtcg caatcggacg cgtggggcgt cctatcttct
    12181 ctggttggtc ttccctgctg ttcctttcta atccatacct aatctcgaca ggggttctcg
    12241 aacctttgga accttttccg gagtagtggc tctctttatt cggtggaaat actggccgga
    12301 atcatttgag tggttgaata gccctttgat gctatggtgc ctaacagtct ccttgttgtt
    12361 tgatggatcc tacggtgttt gcttttggta cgtcaagcgg tccgaagaga tgcttcttgc
    12421 ccagcgaaag gaaagataac ttggatgcaa ttccggctgc atatgtgacc aatctcccca
    12481 gcccgatctt caataactag ttttagtccc aaaaatctag ttttcttagt gtaatatcgg
    12541 gtattagaaa caattctatt gggcctgttc tatgcgcacc tgcaagccag aggcagtacg
    12601 gtatctggag ccgtacccat acgcttgctt ctttttcttt tttttttttt tctctttttc
    12661 cccctttttt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12781 nnnnnnnnnn gtgatttctg ctgtttgggt cgattgagaa agactgcaga taatgaccgt
    12841 gcaaattttc agcgtgtccc ttggaccagg gccctgctac cattcggatt agcttagatt
    12901 ttagtagttg cgccttcctt gcagccaaca tcattgacat tcaacaccgc agcgatgggg
    12961 gagcagacct tcaaagtcat tatcgtcggg ggttcgattg ccggattgac gctcgcacat
    13021 tgtttggagc gaataggaat cgattatctc gtcctggaga aggcgagtga gattgcgccg
    13081 caggtaggag catctatcgg ggtcttcccc aatggcgctc gcatcctaga ccagctagga
    13141 atatacgggg atctcgagga ggtgatagag cccatggggc aagccaccgt ctgttatccc
    13201 gacgggttca gtttcagctc gtcttatccg cagattatcc ataaaaggta gaataccccc
    13261 tgtaatcatc tggttgaagg aatgaccctt aggctgaggg tgacaagcag attccagtat
    13321 ccgatcgcct ttctagaccg acagaaactc ctcgagatac tgtacactaa gcttccggat
    13381 cggagcaaga ttcgtcttcg ctcccgagtt gcatcgataa cgacactccg tgacaatggc
    13441 ggcgtcaacg tgaccacgga cgatgcgcaa acgtacaccg gccatttggt agtgggtgcg
    13501 gatggagtgc acagccgcgt acgctcggag atgtggaagg cagcccagag cgatggacga
    13561 gggccaaccg caaaggagca gcaaggcatg acaacggagt attgctgcat attcggcatc
    13621 tcttcagcag tcaagggact aaacgtggga gaacaggtca acgcatttta cgatcacttg
    13681 accattatga ccgtccacgg taggaagggt cgaatatact ggtttttcat ccagaagctg
    13741 gatcaaaggt atatatatcc tgacaatccc aattatggcc ctgagcatgc aacactggtc
    13801 gccgagcgct ttcgacatag gcgcttgtgc aatgatatca ccttcggcca tgtctgggac
    13861 aacagagaga tcgcgacaat cactccactg gaggagggtc tgttccagac ctggtatcat
    13921 ggtcgcatcg ttctgatggg cgacagcgtc cacaaggtaa gtgatttcga agcttgcatt
    13981 gctcacaatg tgcctgacaa gcgtttggac agatgacacc caacctgggc caaggcgcaa
    14041 atatggcaat cgaggacgcg gctagcctgg caagcctgct caacaatctc ctggacggcg
    14101 gagatggtca gcgactcacc cgggagggta tcgaggcaat gttgcggcaa tatcgagact
    14161 ctcgctattc ccgtgtgcgg gccgtctaca atacctcgcg attcctggtg agatttcagg
    14221 cccgtaatgg cctcttcaac accttatttg gccgttacta tgccccctac gccggcgacc
    14281 tgcccgctga tattggatcc aagacaattg cgggcgcaga ggtatgttct ttccttccta
    14341 ttccatcgtc ggcagatgca tgggaccgtt accggagcag gggatggcgt gagtcaatag
    14401 gggcgaagga agtgttcggt ctcgtaatta tcattggatt agcgttgcag tattttggac
    14461 ctattgggtg ccctggtgcc ttttgtgctt catcgcgttt gacttgaata ctactttgcc
    14521 ggcgggagaa atatcagaag atagatcttg taggccggtc atttagtgtt agactatagc
    14581 tgaacgaaat acatttgtca atcgacctct ataaaagtgt taaagtatga ttgtatctca
    14641 caagatacat aagaaagccg tcagccgcat acatcgcctg gcacattggc tatacagtct
    14701 attgttaacc tcattgcaaa aggattgaca tttcacaggt gacactagac agtgctttca
    14761 ctgttctcgg ctctgttaag gtgcagaatg cgaagagggg cattggggtc cagccccatt
    14821 gcaaaagtat gttgacatac ctcacgctcc tctatgaatt gcttcttggc ggcggcgaag
    14881 agccgccaag caagcgcatg cctcttcgaa ttggtcaacc tggatgagtt ggctgccgca
    14941 tccactcgct ttttttggcc agttttagcc ccattctaaa accactatca ctctacggcg
    15001 tcacctggca gggcggacgg tgccgatctg ggtcatgcta agcactatgg ttcgtccagt
    15061 tgcctcggtg ggtacgttat tcgtaaagaa agacgtccct gccatgctca taccttgaga
    15121 caggaccgta ttcgcgaata ctctgaatct gaccattggt cccggagtaa ttattcccgt
    15181 tctgcgggtc atgaagtcac ccaccttaaa gcaaacacct tgctcaaagc aaacaaccta
    15241 cctcgaagca aacaccctac ctcgaagcaa acaacctacc tcgaagcagg agctcattcc
    15301 tcttggtaaa cctagtcgac ttacgttatg ctctacgtgc ctgtgttaca gtgcttcgat
    15361 acccgtggat ggattgttgg gcttcgaacg aactgccgta ggccaagctc cgcatgatag
    15421 cgctctggca ggtgggcacc cattctcctg tcgtgatcga aacttttcta aatacgcacg
    15481 tactatcctg gggagccgag caggttactg gtcatgcgaa acgacgcatt ttgcatggct
    15541 ccatatcagt atcgcccgag cacgcttgcg gtcttcatcc aaatcgttcc ctgtcttatg
    15601 tgggcgctga gcgtcacttt caattgaacc aatacagtgc cttctggctg taaaacaagc
    15661 cctagtcagc tgtgttggcg aagaaagcgt aggtctattc ccgtggagac cccggaaagt
    15721 ccggagttcg taatggctgt gttgggacca aactattaaa cagttcataa tcattttcat
    15781 tggcgctcag tccaagtacg tccactttga taggaactca cgaaccaacc cccttgaaga
    15841 gcaggaggcc caatatccca actctttaca ggctttgaag cgaaccgctc cctgactact
    15901 tcctattccc cgtccatgaa acaataaccc acggtgacac tgaaaaaggt gaggttctcg
    15961 ctagtccaag tcctgtgaac cgcgaacatt gactcaaact gattttaacc agatgggcag
    16021 cactgtttca cttcctcaat ctgtgtccgc tttatgggaa cgcctatcct tcccattccg
    16081 cgtggactat gcaacgaaat cgccgtcttc gacgccctgc aatgaaatta attcacctat
    16141 aacgaaaaat gagcctaccg aagtcttcgg ccctccgaga atttgcaaga agccagtgag
    16201 cttgccgcac agggccccgg tggcgcagga tttggagagg gtcaatccac agctcccaga
    16261 gttggataga cccataagtt accaggatga ggttctacac tttcccgata gtcatggtgg
    16321 atcgttctaa cgcggacagt aacagataat ccgcaggccg ctcgattacc tcctctcctt
    16381 acctggaaag cagctccgca cccaactcgt cctcgcgttc aatgagtggt ttcgcgtacc
    16441 ggacgataag ctgcagctaa ttgttgacgc cactgagctc ctgcatacag cgtccctact
    16501 gtaagtccat ctgagttcta tatcattaat gtagtgcctg attagcttgt agtgtcgatg
    16561 atatccaaga cggctcaaag ctgcggcgcg gccaccccgc cgctcacacg gtctttggtt
    16621 ctgcgcagac aatcaactcg gccaactttg catacttcct cgcccaagaa agactgggcg
    16681 agctaaggag catcgatgcc tttcagatct tcataacgga gctactcaac cttcacaggg
    16741 gtcaaggcct ggagctctac ttccgggatt gcctgatatg tccgacggag gaggaatact
    16801 tggagatagt aacccacaaa acgggtggac tattccggct cgctgtgggt ctactgcagt
    16861 cccagagcga gatatcactg tgagacaccc gaaattggtg agcttgctcc aaagactgat
    16921 tgttgaaagc agcgactgca gggcactggc ggacacgctg ggcgtcatct accagatccg
    16981 ggacgactat ctgaatctgc aaagtgacca gtatgcaaag agcaagggat tcggcgagga
    17041 tctaaccgaa ggcaagtttt catttcccat tgtccacagc atcctgtcgg cgccggaaaa
    17101 tacgaccctg atcgatattc ttggacaacg caccgaagac gaagcggtca aactctcggc
    17161 catccgccac atggagtcga cggggagctt cgagtactgt cgccagcgct taaataccct
    17221 gcagacagag gctattagca tgatcgagca aatagaggac atccaggggc cgggacatgg
    17281 catccgtcgg atattacgtt gtctgatgtt gacttcgtga gtaatggggg atatataatc
    17341 agtgacgctg gcctgattaa acggttacat tcctttcact actttggctt gtttgtcttc
    17401 tctgtaagcg gcttctgtac accgggggcg gtggtcctgg tagtatgcgc tatgaaattt
    17461 gttacttaaa gggttatatc cataccattg taggcagtta accggctaaa tgctgttttc
    17521 cggcgtcaag gtttcatgat gttgtggata cgccttcaaa ggggaaaggg cagaaggaag
    17581 ctgtatcaac tacaccaacc tggcctacta aggatcggac gccacatgac gattgattat
    17641 ctttctcttt gttgttcttt cttctcctcc cttcaatctc tctcctttcc cccactatat
    17701 atacatatag gagggtgaga accttctaga cctaggcgat atccggtcaa tccaccggag
    17761 atcacgtggc cttgtatttc cgtatcactc cccttcgttc aataccccga aacgaacggc
    17821 atgaagctac acaagcttcc aaaacaggaa actaaccaaa acaagtggag gggtgttgaa
    17881 cgcctttatc ctatcagtct gcggatcttg atgttgtatc atagaacctt attttgactc
    17941 cagctgcagc aattgaagtg cttgctttaa gccatctgct ttgactgctt ttgtcaatcc
    18001 gtctgcaacc attctggagg taggaagata tatggcttga tatctccatg ctcaatgaaa
    18061 tctcggatgg catgatatcg tatgcggata tgcttgctgc gtaggtggtt aactgggttg
    18121 gacgctagcg ataaggtacc ttgattgtca atccccaact gagttggctg cttggggtgc
    18181 ttccgaatag ccgctaggaa gtgtctaatc cagatagcct gctttgctgc ctctgcagct
    18241 gcaatatatt ccgcttcagt ggttgacata gcagtaatag gttgcttcct gctacaccaa
    18301 ctaactggtc cgttagccag cataaagacg tac
//
