BGC0002868: Lenziamide A biosynthetic gene cluster from Streptomyces sp PC5
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Location: 1 - 58,983 nt. (total: 58,983 nt).
This entry is originally from NCBI GenBank OR050662.1.

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core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
TTA codons
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General
Compounds
Genes
Biosynthesis
History
NRPS/PKS domains
KnownClusterBlast
General information about the BGC
MIBiG accession BGC0002868
Short description Lenziamide A biosynthetic gene cluster from Streptomyces sp PC5
Status Quality: questionable
The quality level of this entry.

Status: pending
The status of this entry.

Completeness: complete
Whether the entry covers everything needed for the pathway producing the compound(s)
Remarks "There are two strains that produce the same derivatives. Since the two gene sequences are slightly different, both were entered separately. And I submitted new041 entry incorrectly, so I quit it."
Biosynthetic class(es)
  • NRPS (Type I)
Loci
OR050662.1
1 - 58983
via Homology-based prediction [2]
Compounds
  • Lenziamide A
Species Streptomyces sp PC5 [taxonomy]
References
Chemical products information
Lenziamide A Evidence:
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C57H87N11O11
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • WMP36085.1
  • orf1
 1 - 669 (-) ABC transporter
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    • WMP36086.1
    • orf2
    		 666 - 1568 (-) ABC transporter
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      • WMP36087.1
      • orf3
      		 1586 - 2260 (-) AIM24 family protein
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        • WMP36088.1
        • orf4
        		 2332 - 3081 (+) peptidyl-tRNA hydrolase
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          • WMP36089.1
          • orf9
          		 3296 - 3508 (-) MbtH family protein
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            • WMP36090.1
            • orf10
            		 3604 - 4374 (-) thioesterase
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              • WMP36091.1
              • orf11
              		 5015 - 6019 (+) ParB N-terminal domain-containing protein
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                • WMP36092.1
                • lnzpA
                		 6103 - 6510 (-) aspartate 1-decarboxylase
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                  • WMP36093.1
                  • lnzpB
                  		 6492 - 7250 (-) N-N bond formation enzyme (KztT)
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                    • WMP36094.1
                    • lnzpC
                    		 7237 - 8595 (-) L-ornithine N-hydroxylase (KtzI)
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                      • WMP36095.1
                      • lnzpD
                      		 9020 - 46081 (+) Lenziamides core NRPS
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                        • WMP36096.1
                        • orf12
                        		 46350 - 47099 (+) DUF4142 domain-containing protein
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                          • WMP36097.1
                          • orf13
                          		 47221 - 48549 (+) DUF692 domain-containing protein
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                            • WMP36098.1
                            • orf14
                            		 48555 - 49610 (+) TIGR04222 domain-containing membrane protein
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                              • WMP36099.1
                              • orf15
                              		 49760 - 51322 (+) alpha/beta hydrolase
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                                • WMP36100.1
                                • orf16
                                		 51479 - 52207 (-) chlorite dismutase family protein
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                                  • WMP36101.1
                                  • orf17
                                  		 52212 - 53675 (-) protoporphyrinogen oxidase
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                                    • WMP36102.1
                                    • orf18
                                    		 53802 - 54767 (+) DUF4349 domain-containing protein
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                                      • WMP36103.1
                                      • orf19
                                      		 54834 - 56225 (+) FAD-dependent oxidoreductase
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                                        • WMP36104.1
                                        • orf20
                                        		 56256 - 57353 (-) uroporphyrinogen decarboxylase
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                                          • WMP36105.1
                                          • orf21
                                          		 57524 - 58108 (+) DUF3000 domain-containing protein
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                                            • WMP36106.1
                                            • orf22
                                            		 58321 - 58983 (+) response regulator transcription factor
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                                              Biosynthesis information

                                              Biosynthetic paths

                                              beta-L-alanine
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                                              Pathway: lnzpA [2]
                                              Comment: LnzpA is an aspartate 1- decarboxylase, which catalyzes the decarboxylation of aspartate to produce β-alanine.
                                              Precursor
                                              L-piperazic acid
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                                              Pathway: lnzpC > lnzpB [1], [3]
                                              Comment: LnzpC and LnzpB are homologous to KtzI and KtzT, respectively.
                                              Precursor
                                              Lenziamide A
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                                              Pathway: lnzpD [2]
                                              Comment: Final product is biosynthesized by NRPS LnzpD.

                                              Biosynthetic modules

                                              Name
                                              1
                                              Type
                                              nrps-type1
                                              Genes
                                              lnzpD
                                              Substrates
                                              alanine (evidence: Sequence-based prediction [2])
                                              Integrated Monomers
                                              Domains
                                              adenylation, carrier (PCP)
                                              Name
                                              2
                                              Type
                                              nrps-type1
                                              Genes
                                              lnzpD
                                              Substrates
                                              phenylalanine (evidence: Sequence-based prediction [2])
                                              Integrated Monomers
                                              Domains
                                              condensation (LCL), adenylation, methyltransferase (N), carrier (PCP)
                                              Name
                                              3
                                              Type
                                              nrps-type1
                                              Genes
                                              lnzpD
                                              Substrates
                                              leucine (evidence: Sequence-based prediction [2])
                                              Integrated Monomers
                                              Domains
                                              condensation (LCL), adenylation, carrier (PCP)
                                              Name
                                              4
                                              Type
                                              nrps-type1
                                              Genes
                                              lnzpD
                                              Substrates
                                              serine (evidence: Sequence-based prediction [2])
                                              Integrated Monomers
                                              Domains
                                              condensation (LCL), adenylation, methyltransferase (N), carrier (PCP)
                                              Name
                                              5
                                              Type
                                              nrps-type1
                                              Genes
                                              lnzpD
                                              Substrates
                                              L-piperazic acid (evidence: Sequence-based prediction [2])
                                              Integrated Monomers
                                              Domains
                                              condensation (LCL), adenylation, carrier (PCP)
                                              Name
                                              6
                                              Type
                                              nrps-type1
                                              Genes
                                              lnzpD
                                              Substrates
                                              phenylalanine (evidence: Sequence-based prediction [2])
                                              Integrated Monomers
                                              Domains
                                              condensation (LCL), adenylation, methyltransferase (N), carrier (PCP)
                                              Name
                                              7
                                              Type
                                              nrps-type1
                                              Genes
                                              lnzpD
                                              Substrates
                                              isoleucine (evidence: Sequence-based prediction [2])
                                              Integrated Monomers
                                              Domains
                                              condensation (LCL), adenylation, carrier (PCP)
                                              Name
                                              8
                                              Type
                                              nrps-type1
                                              Genes
                                              lnzpD
                                              Substrates
                                              alanine (evidence: Sequence-based prediction [2])
                                              Integrated Monomers
                                              Domains
                                              condensation (LCL), adenylation, methyltransferase (N), carrier (PCP)
                                              Name
                                              9
                                              Type
                                              nrps-type1
                                              Genes
                                              lnzpD
                                              Substrates
                                              beta-alanine (evidence: Sequence-based prediction [2])
                                              Integrated Monomers
                                              Domains
                                              condensation (LCL), adenylation, carrier (PCP)
                                              Name
                                              10
                                              Type
                                              nrps-type1
                                              Genes
                                              lnzpD
                                              Substrates
                                              valine (evidence: Sequence-based prediction [2])
                                              Integrated Monomers
                                              Domains
                                              condensation (LCL), adenylation, methyltransferase (N), carrier (PCP)
                                              Annotation changelog

                                              Entry version: next

                                              Date
                                              Changes
                                              Submitters
                                              Reviewers
                                              2024-05-20
                                              MIBiG v4 annotathon
                                              • (ID: 6NSEHDSBVKYTX3SHWHOOIJMO)
                                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
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