BGC0002958 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0002958 (Streptomyces sp)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS	1	59,411	Medium	rosamicin/salinipyrone A/pacificanone A	PKS

BGC0002958 - Region 1 - T1PKS

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Location: 1 - 59,411 nt. (total: 59,411 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 59,412
Label		BGC0002958

Tailoring functions

Oxidoreductases: oxygenase

desVIII: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

pikC: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

Transferases: aminotransferase glycosyltransferase kinase methyltransferase transferase

desI: DegT/DnrJ/EryC1/StrS aminotransferase

smcogs: SMCOG1056: DegT/DnrJ/EryC1/StrS aminotransferase

desIII: glucose-1-phosphate adenylyl/thymidylyltransferase

smcogs: SMCOG1064: glucose-1-phosphate adenylyl/thymidylyltransferase

desV: DegT/DnrJ/EryC1/StrS aminotransferase

smcogs: SMCOG1056: DegT/DnrJ/EryC1/StrS aminotransferase

desVI: methyltransferase

smcogs: SMCOG1248: methyltransferase

desVII: glycosyltransferase, MGT family

smcogs: SMCOG1062: glycosyltransferase, MGT family

orf21: PfkB domain protein

smcogs: SMCOG1276: PfkB domain protein

Hydrolases: esterase

pikAV: thioesterase

smcogs: SMCOG1004: thioesterase

Lyases: dehydratase

desIV: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

Ligases: synthase

pikAI: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

pikAII: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

pikAIII: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

pikAIV: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000898.5	0.95	saccharide	desosamine, pikromycin, neomethymycin, narbomycin	Streptomyces venezuelae
BGC0000094.5	0.93	PKS, saccharide	methymycin	Streptomyces venezuelae
BGC0000899.4	0.82	saccharide	desosamine	Streptomyces narbonensis
BGC0001273.3	0.76	PKS	asperlactone	Aspergillus ochraceus
BGC0001244.3	0.76	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001275.3	0.75	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0000041.3	0.73	PKS	oronofacic acid	Pseudomonas syringae
BGC0001276.3	0.67	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0002154.2	0.66	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001257.3	0.66	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000898.5	0.95	saccharide	desosamine, pikromycin, neomethymycin, narbomycin	Streptomyces venezuelae
BGC0000094.5	0.93	PKS, saccharide	methymycin	Streptomyces venezuelae
BGC0000899.4	0.82	saccharide	desosamine	Streptomyces narbonensis
BGC0001273.3	0.76	PKS	asperlactone	Aspergillus ochraceus
BGC0001244.3	0.76	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001275.3	0.75	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0000041.3	0.73	PKS	oronofacic acid	Pseudomonas syringae
BGC0001276.3	0.67	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0002154.2	0.66	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001257.3	0.66	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 58927:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (Me-mal - Me-ccmal - ccmal) + (Me-ohmal - Me-redmal) + (Me-ohmal) + (Me-mal)
Non-colinear ordering:: pikAI + pikAII + pikAIII + pikAIV

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

pikAI: mmal - mmal - mal

PKS_AT (548..832): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 210.7
Ethylmalonyl-CoA: 185.1
Methoxymalonyl-CoA: 157.2
2-Methylbutyryl-CoA: 134.2
Propionyl-CoA: 108.1
trans-1,2-CPDA: 99.9
CHC-CoA: 88.8
Malonyl-CoA: 84.7
Isobutyryl-CoA: 53.4
Acetyl-CoA: 43.1
Benzoyl-CoA: 39.6
fatty_acid: 37.1
3-Methylbutyryl-CoA: 19.3
inactive: 13.3

PKS_AT (1555..1839): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 210.7
Ethylmalonyl-CoA: 185.1
Methoxymalonyl-CoA: 157.2
2-Methylbutyryl-CoA: 134.2
Propionyl-CoA: 108.1
trans-1,2-CPDA: 99.9
CHC-CoA: 88.8
Malonyl-CoA: 84.7
Isobutyryl-CoA: 53.4
Acetyl-CoA: 43.1
Benzoyl-CoA: 39.6
fatty_acid: 37.1
3-Methylbutyryl-CoA: 19.3
inactive: 13.3

PKS_AT (3078..3371): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 87.5%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 232.8
inactive: 116.9
Methoxymalonyl-CoA: 79.7
Methylmalonyl-CoA: 73.8
Propionyl-CoA: 44.1
Isobutyryl-CoA: 41.0
Ethylmalonyl-CoA: 36.9
2-Methylbutyryl-CoA: 31.2
Acetyl-CoA: 29.6
Benzoyl-CoA: 20.3
CHC-CoA: 19.0
fatty_acid: 16.9
trans-1,2-CPDA: 16.1
3-Methylbutyryl-CoA: 0.0

pikAII: mmal - mmal

PKS_AT (572..856): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 206.6
Ethylmalonyl-CoA: 181.0
Methoxymalonyl-CoA: 156.5
2-Methylbutyryl-CoA: 125.5
Propionyl-CoA: 103.2
trans-1,2-CPDA: 98.7
CHC-CoA: 84.6
Malonyl-CoA: 73.0
Isobutyryl-CoA: 45.3
fatty_acid: 36.8
Benzoyl-CoA: 33.0
Acetyl-CoA: 32.9
3-Methylbutyryl-CoA: 14.3
inactive: 9.0

PKS_AT (2062..2346): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 209.1
Ethylmalonyl-CoA: 183.8
Methoxymalonyl-CoA: 158.2
2-Methylbutyryl-CoA: 131.2
Propionyl-CoA: 110.2
trans-1,2-CPDA: 103.2
CHC-CoA: 87.6
Malonyl-CoA: 85.4
Isobutyryl-CoA: 43.7
Acetyl-CoA: 37.6
fatty_acid: 36.9
Benzoyl-CoA: 30.8
3-Methylbutyryl-CoA: 19.5
inactive: 13.2

pikAIII: mmal

PKS_AT (565..846): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 204.3
Ethylmalonyl-CoA: 180.9
Methoxymalonyl-CoA: 154.2
2-Methylbutyryl-CoA: 127.6
Propionyl-CoA: 106.4
trans-1,2-CPDA: 97.7
CHC-CoA: 87.4
Malonyl-CoA: 71.1
Isobutyryl-CoA: 41.2
fatty_acid: 32.2
Acetyl-CoA: 29.1
Benzoyl-CoA: 27.6
inactive: 13.3
3-Methylbutyryl-CoA: 12.2

pikAIV: mmal

PKS_AT (526..809): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 204.6
Ethylmalonyl-CoA: 180.3
Methoxymalonyl-CoA: 153.3
2-Methylbutyryl-CoA: 120.3
Propionyl-CoA: 102.3
trans-1,2-CPDA: 100.1
CHC-CoA: 87.0
Malonyl-CoA: 69.1
Isobutyryl-CoA: 43.3
Benzoyl-CoA: 31.9
fatty_acid: 31.4
Acetyl-CoA: 27.6
inactive: 12.0
3-Methylbutyryl-CoA: 10.7

Link to PARAS substrate predictor