BGC0002964 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0002964 (Streptomyces peucetius subsp. caesius ATCC 27952)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS	1	68,256	Low	tiacumicin B	PKS:Type I

BGC0002964 - Region 1 - T1PKS

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Location: 1 - 68,256 nt. (total: 68,256 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 68,257
Label		BGC0002964

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase oxygenase

CGZ69_08235: flavin reductase domain protein FMN-binding

smcogs: SMCOG1160: flavin reductase domain protein FMN-binding

CGZ69_08250: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

CGZ69_08255: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

CGZ69_08265: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

CGZ69_08300: Hydroxylation

MITE: MITE0000147Hydroxylation (identity: 100.00%)
smcogs: SMCOG1083: oxidoreductase

CGZ69_08305: oxidoreductase

smcogs: SMCOG1083: oxidoreductase

Transferases: acylase

CGZ69_08285: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

Hydrolases: esterase hydrolase

CGZ69_08245: beta-lactamase

smcogs: SMCOG1053: beta-lactamase

CGZ69_08270: thioesterase

smcogs: SMCOG1004: thioesterase

Ligases: synthase

CGZ69_08275: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

CGZ69_08280: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

CGZ69_08290: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

CGZ69_08295: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001273.3	0.78	PKS	asperlactone	Aspergillus ochraceus
BGC0001276.3	0.78	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001275.3	0.77	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.76	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0000041.3	0.70	PKS	oronofacic acid	Pseudomonas syringae
BGC0001284.4	0.68	PKS	alternariol	Parastagonospora nodorum SN15
BGC0002191.2	0.67	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001254.3	0.66	PKS	ACT-Toxin II	Alternaria alternata
BGC0001164.3	0.65	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0002192.3	0.65	PKS	FR901512	fungal sp. No.14919

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001273.3	0.78	PKS	asperlactone	Aspergillus ochraceus
BGC0001276.3	0.78	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001275.3	0.77	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.76	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0000041.3	0.70	PKS	oronofacic acid	Pseudomonas syringae
BGC0001284.4	0.68	PKS	alternariol	Parastagonospora nodorum SN15
BGC0002191.2	0.67	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001254.3	0.66	PKS	ACT-Toxin II	Alternaria alternata
BGC0001164.3	0.65	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0002192.3	0.65	PKS	FR901512	fungal sp. No.14919

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 68256:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (Me-mal - ccemal - Me-ccmal) + (ccmal - ccmal) + (emal)
Non-colinear ordering:: CGZ69_08295 + CGZ69_08290 + CGZ69_08285

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

CGZ69_08285: emal

PKS_AT (568..866): emal

ATSignature: Methoxymalonyl-CoA, Ethylmalonyl-CoA

Top 3 matches:: Methoxymalonyl-CoA: 87.5%; Ethylmalonyl-CoA: 87.5%; Methylmalonyl-CoA: 83.3%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 213.3
Methylmalonyl-CoA: 195.8
Methoxymalonyl-CoA: 174.1
2-Methylbutyryl-CoA: 155.8
trans-1,2-CPDA: 129.1
Malonyl-CoA: 109.1
Propionyl-CoA: 106.3
CHC-CoA: 105.6
Isobutyryl-CoA: 90.8
Benzoyl-CoA: 58.5
Acetyl-CoA: 48.1
3-Methylbutyryl-CoA: 38.6
inactive: 34.5
fatty_acid: 33.8

CGZ69_08290: mal - mal

PKS_AT (575..871): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 83.3%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 213.0
inactive: 103.9
Methoxymalonyl-CoA: 60.1
Methylmalonyl-CoA: 57.7
Benzoyl-CoA: 39.5
Ethylmalonyl-CoA: 30.0
Isobutyryl-CoA: 26.5
Propionyl-CoA: 26.1
fatty_acid: 25.9
trans-1,2-CPDA: 15.0
CHC-CoA: 14.1
Acetyl-CoA: 11.3
2-Methylbutyryl-CoA: 9.1
3-Methylbutyryl-CoA: 0.0

PKS_AT (2324..2620): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 83.3%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 213.0
inactive: 103.9
Methoxymalonyl-CoA: 60.1
Methylmalonyl-CoA: 57.7
Benzoyl-CoA: 39.5
Ethylmalonyl-CoA: 30.0
Isobutyryl-CoA: 26.5
Propionyl-CoA: 26.1
fatty_acid: 25.9
trans-1,2-CPDA: 15.0
CHC-CoA: 14.1
Acetyl-CoA: 11.3
2-Methylbutyryl-CoA: 9.1
3-Methylbutyryl-CoA: 0.0

CGZ69_08295: mmal - emal - mmal

PKS_AT (561..861): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Methoxymalonyl-CoA: 87.5%; Ethylmalonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 206.2
Ethylmalonyl-CoA: 204.5
Methoxymalonyl-CoA: 159.7
2-Methylbutyryl-CoA: 147.7
trans-1,2-CPDA: 128.4
Propionyl-CoA: 106.7
CHC-CoA: 86.9
Malonyl-CoA: 84.6
Isobutyryl-CoA: 68.5
Benzoyl-CoA: 67.1
fatty_acid: 31.2
Acetyl-CoA: 29.7
inactive: 25.9
3-Methylbutyryl-CoA: 23.3

PKS_AT (1589..1887): emal

ATSignature: Ethylmalonyl-CoA

Top 3 matches:: Ethylmalonyl-CoA: 95.8%; Methoxymalonyl-CoA: 83.3%; Methylmalonyl-CoA: 79.2%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 195.5
Methylmalonyl-CoA: 191.3
Methoxymalonyl-CoA: 151.7
2-Methylbutyryl-CoA: 121.6
Propionyl-CoA: 95.8
trans-1,2-CPDA: 95.2
Malonyl-CoA: 88.9
CHC-CoA: 75.0
Isobutyryl-CoA: 68.1
Benzoyl-CoA: 42.5
3-Methylbutyryl-CoA: 38.9
Acetyl-CoA: 35.2
fatty_acid: 28.4
inactive: 22.6

PKS_AT (3364..3662): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 91.7%; Methoxymalonyl-CoA: 87.5%; Ethylmalonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 210.4
Ethylmalonyl-CoA: 199.1
Methoxymalonyl-CoA: 176.4
2-Methylbutyryl-CoA: 137.2
trans-1,2-CPDA: 114.1
Propionyl-CoA: 109.4
Malonyl-CoA: 89.5
CHC-CoA: 87.9
Isobutyryl-CoA: 70.1
Benzoyl-CoA: 50.7
Acetyl-CoA: 40.7
3-Methylbutyryl-CoA: 40.2
fatty_acid: 36.8
inactive: 25.9

Link to PARAS substrate predictor