BGC0001042 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001042 (Streptomyces flaveolus)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	NRPS,T1PKS,hglE-KS,PKS-like	1	118,372	lasalocid	Polyketide	40%

BGC0001042 - Region 1 - NRPS,PKS-like,T1PKS,hglE-KS

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Location: 1 - 118,372 nt. (total: 118,372 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
hglE-KS: (hglE or hglD)
PKS-like: (t2pks2 or ksIII)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

External annotation

Name		MIBiG
Version		3.0

Type		Subregion
Location		1 - 118,373
Label		BGC0001042

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001042.1	1.80	NRP, Polyketide	sanglifehrin A	Streptomyces flaveolus
BGC0000041.1	1.28	Polyketide	oronofacic acid	Pseudomonas syringae
BGC0000706.1	1.07	Saccharide	kanamycin	Streptomyces kanamyceticus
BGC0000019.1	1.03	Polyketide	angolamycin	Streptomyces eurythermus
BGC0000013.1	1.01	Polyketide	alternariol	Aspergillus nidulans FGSC A4
BGC0000056.1	0.96	Polyketide	esperamicin	Actinomadura verrucosospora
BGC0000067.1	0.96	Polyketide	geldanamycin	Streptomyces hygroscopicus subsp. duamyceticus
BGC0001144.1	0.94	Polyketide	neosartoricin B	Trichophyton tonsurans CBS 112818
BGC0000099.1	0.93	Polyketide	monascorubrin	Talaromyces marneffei
BGC0000863.1	0.93	Other	eicosapentaenoic acid	Pseudoalteromonas sp. DS-12

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001042.1	0.95	NRP, Polyketide	sanglifehrin A	Streptomyces flaveolus
BGC0000067.1	0.68	Polyketide	geldanamycin	Streptomyces hygroscopicus subsp. duamyceticus
BGC0000041.1	0.67	Polyketide	oronofacic acid	Pseudomonas syringae
BGC0000706.1	0.65	Saccharide	kanamycin	Streptomyces kanamyceticus
BGC0001504.1	0.65	Polyketide	amycomicin	Amycolatopsis sp. AA4
BGC0000863.1	0.64	Other	eicosapentaenoic acid	Pseudoalteromonas sp. DS-12
BGC0000113.1	0.64	Polyketide	niddamycin	Streptomyces caelestis
BGC0001273.1	0.64	Polyketide	asperlactone	Aspergillus ochraceus
BGC0001164.1	0.64	Polyketide	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001276.1	0.64	Polyketide	6-methylsalicyclic acid	Aspergillus terreus

Similar known gene clusters

Module view

Legend

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 118372:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (val - X - pro) + (Me-redmal) + (Me-mal - Me-ccmal - ohmal) + (Me-ohmal - redmal) + (ccmal - Me-ccmal - ohmal - ccmal - ccmal) + (Me-ohmal - ccemal) + (mal)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 0 - 116040:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (val - X - pro) + (Me-redmal) + (Me-mal - Me-ccmal - ohmal) + (Me-ohmal - redmal) + (ccmal - Me-ccmal - ohmal - ccmal - ccmal) + (Me-ohmal - ccemal) + (mal)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 77395 - 118372:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (mal) + (ccmal - Me-ccmal - ohmal - ccmal - ccmal) + (Me-ohmal - ccemal)
Non-colinear ordering:: sfaK + sfaH + sfaI

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 4, location 84000 - 118372:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (mal) + (ccmal - Me-ccmal - ohmal - ccmal - ccmal) + (Me-ohmal - ccemal)
Non-colinear ordering:: sfaK + sfaH + sfaI

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ACY06269.1

CAL_domain (31..464): pk

minowa: fatty_acid

Prediction, score:

fatty_acid: 23.6
AHBA: 16.3
shikimic_acid: 9.1
NH2: 5.9
Acetyl-CoA: 0.0

sfaD

Search NORINE for peptide: strict or relaxed

AMP-binding (498..892): val

NRPSPredictor2: val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: val

Stachelhaus prediction details:

Stachelhaus sequence:: DAYWvGGTFK
Nearest Stachelhaus code:: val
Stachelhaus code match:: 90% (moderate)

AMP-binding (1537..1927): X

NRPSPredictor2: gly, ala, val, leu, ile, abu, iva

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DiycLGaAfK
Nearest Stachelhaus code:: pip
Stachelhaus code match:: 50% (weak)

AMP-binding (2579..2980): pro

NRPSPredictor2: pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: pro, pip
Small clusters prediction:: pro
Single AA prediction:: pro

Stachelhaus prediction details:

Stachelhaus sequence:: DVQFsAHVVK
Nearest Stachelhaus code:: pro
Stachelhaus code match:: 90% (moderate)

sfaE

PKS_AT (652..949): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 218.4
Ethylmalonyl-CoA: 208.0
Methoxymalonyl-CoA: 182.4
trans-1,2-CPDA: 137.9
2-Methylbutyryl-CoA: 137.4
Isobutyryl-CoA: 105.5
CHC-CoA: 105.2
Propionyl-CoA: 103.3
Malonyl-CoA: 95.1
Benzoyl-CoA: 90.9
Acetyl-CoA: 55.6
3-Methylbutyryl-CoA: 49.5
fatty_acid: 36.8
inactive: 24.0

sfaF

PKS_AT (563..857): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 222.9
Ethylmalonyl-CoA: 209.2
Methoxymalonyl-CoA: 183.0
2-Methylbutyryl-CoA: 142.9
trans-1,2-CPDA: 134.5
CHC-CoA: 115.5
Propionyl-CoA: 113.7
Malonyl-CoA: 109.1
Isobutyryl-CoA: 104.8
Benzoyl-CoA: 82.8
Acetyl-CoA: 55.1
3-Methylbutyryl-CoA: 46.2
inactive: 31.1
fatty_acid: 27.1

PKS_AT (1573..1868): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 218.9
Ethylmalonyl-CoA: 206.6
Methoxymalonyl-CoA: 180.7
2-Methylbutyryl-CoA: 152.3
trans-1,2-CPDA: 127.9
CHC-CoA: 119.2
Propionyl-CoA: 115.8
Malonyl-CoA: 113.5
Isobutyryl-CoA: 96.2
Benzoyl-CoA: 68.9
3-Methylbutyryl-CoA: 42.5
Acetyl-CoA: 41.2
fatty_acid: 34.1
inactive: 32.4

PKS_AT (3230..3500): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 75.0%; Methylmalonyl-CoA: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 184.2
inactive: 83.7
Methoxymalonyl-CoA: 65.7
Methylmalonyl-CoA: 59.1
Isobutyryl-CoA: 36.5
Propionyl-CoA: 33.3
fatty_acid: 22.9
Benzoyl-CoA: 20.1
trans-1,2-CPDA: 19.7
Acetyl-CoA: 18.1
2-Methylbutyryl-CoA: 16.6
CHC-CoA: 7.5
Ethylmalonyl-CoA: -3.1
3-Methylbutyryl-CoA: -3.9

sfaG

PKS_AT (561..850): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 197.2
Ethylmalonyl-CoA: 182.0
Methoxymalonyl-CoA: 172.2
2-Methylbutyryl-CoA: 120.2
Malonyl-CoA: 106.6
trans-1,2-CPDA: 102.2
Propionyl-CoA: 100.3
CHC-CoA: 93.9
Benzoyl-CoA: 67.6
Isobutyryl-CoA: 53.6
Acetyl-CoA: 37.0
3-Methylbutyryl-CoA: 35.1
fatty_acid: 25.5
inactive: 18.9

PKS_AT (2049..2344): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 79.2%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 188.6
inactive: 89.3
Methoxymalonyl-CoA: 72.9
Methylmalonyl-CoA: 60.5
Propionyl-CoA: 39.8
Ethylmalonyl-CoA: 22.9
Acetyl-CoA: 22.8
Isobutyryl-CoA: 22.1
2-Methylbutyryl-CoA: 19.5
Benzoyl-CoA: 19.1
CHC-CoA: 17.9
fatty_acid: 14.8
trans-1,2-CPDA: 6.7
3-Methylbutyryl-CoA: 0.0

sfaH

PKS_AT (558..844): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 75.0%; Methylmalonyl-CoA: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 180.8
inactive: 83.8
Methoxymalonyl-CoA: 80.3
Methylmalonyl-CoA: 66.6
Isobutyryl-CoA: 54.7
Ethylmalonyl-CoA: 41.6
Propionyl-CoA: 38.3
2-Methylbutyryl-CoA: 28.1
fatty_acid: 21.4
Benzoyl-CoA: 18.7
3-Methylbutyryl-CoA: 15.1
Acetyl-CoA: 12.6
trans-1,2-CPDA: 11.6
CHC-CoA: 0.0

PKS_AT (2254..2548): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 224.7
Ethylmalonyl-CoA: 209.2
Methoxymalonyl-CoA: 185.9
2-Methylbutyryl-CoA: 150.8
trans-1,2-CPDA: 129.3
CHC-CoA: 116.9
Propionyl-CoA: 109.4
Malonyl-CoA: 106.2
Isobutyryl-CoA: 104.7
Benzoyl-CoA: 87.3
Acetyl-CoA: 55.8
3-Methylbutyryl-CoA: 53.3
inactive: 31.9
fatty_acid: 31.7

PKS_AT (3928..4198): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 75.0%; Methylmalonyl-CoA: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 174.2
inactive: 80.3
Methoxymalonyl-CoA: 68.3
Methylmalonyl-CoA: 62.8
Isobutyryl-CoA: 38.0
Propionyl-CoA: 37.4
fatty_acid: 25.5
Benzoyl-CoA: 22.8
trans-1,2-CPDA: 21.6
Acetyl-CoA: 19.0
2-Methylbutyryl-CoA: 17.3
CHC-CoA: 9.9
Ethylmalonyl-CoA: -3.1
3-Methylbutyryl-CoA: -3.6

PKS_AT (5378..5648): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 75.0%; Methylmalonyl-CoA: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 176.9
inactive: 84.8
Methoxymalonyl-CoA: 69.4
Methylmalonyl-CoA: 64.1
Isobutyryl-CoA: 38.7
Propionyl-CoA: 35.4
Benzoyl-CoA: 25.9
fatty_acid: 25.6
trans-1,2-CPDA: 23.4
2-Methylbutyryl-CoA: 22.7
Acetyl-CoA: 18.5
CHC-CoA: 8.8
3-Methylbutyryl-CoA: 0.0
Ethylmalonyl-CoA: -3.1

PKS_AT (7048..7335): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 79.2%; Propionyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 193.8
inactive: 100.2
Methoxymalonyl-CoA: 60.9
Methylmalonyl-CoA: 56.4
Propionyl-CoA: 37.0
Isobutyryl-CoA: 32.6
Ethylmalonyl-CoA: 29.6
2-Methylbutyryl-CoA: 24.2
fatty_acid: 23.2
Benzoyl-CoA: 21.7
Acetyl-CoA: 14.5
CHC-CoA: 13.7
trans-1,2-CPDA: 5.7
3-Methylbutyryl-CoA: 0.0

sfaI

PKS_AT (631..924): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 213.4
Ethylmalonyl-CoA: 207.5
Methoxymalonyl-CoA: 173.8
2-Methylbutyryl-CoA: 152.1
trans-1,2-CPDA: 124.5
Propionyl-CoA: 120.8
Malonyl-CoA: 111.2
CHC-CoA: 107.1
Isobutyryl-CoA: 83.1
Benzoyl-CoA: 53.1
3-Methylbutyryl-CoA: 48.2
Acetyl-CoA: 39.8
inactive: 36.9
fatty_acid: 28.1

PKS_AT (2188..2486): emal

ATSignature: Ethylmalonyl-CoA, Methoxymalonyl-CoA

Top 3 matches:: Ethylmalonyl-CoA: 87.5%; Methoxymalonyl-CoA: 87.5%; Methylmalonyl-CoA: 83.3%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 203.9
Methylmalonyl-CoA: 184.1
Methoxymalonyl-CoA: 158.5
trans-1,2-CPDA: 119.3
2-Methylbutyryl-CoA: 114.2
Propionyl-CoA: 90.8
Malonyl-CoA: 83.8
Isobutyryl-CoA: 83.1
CHC-CoA: 78.2
Benzoyl-CoA: 72.0
Acetyl-CoA: 38.2
fatty_acid: 33.0
3-Methylbutyryl-CoA: 20.0
inactive: 15.7

sfaK

PKS_AT (542..828): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 75.0%; inactive: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 54.0
Methylmalonyl-CoA: 21.5
Methoxymalonyl-CoA: 20.9
inactive: 16.3
Ethylmalonyl-CoA: 3.8
CHC-CoA: 3.2
fatty_acid: 2.8
Isobutyryl-CoA: 2.2
Propionyl-CoA: 0.5
trans-1,2-CPDA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

sfaL

PKS_AT (120..329): mal

ATSignature: (unknown)

No matches above 50%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 0.5
trans-1,2-CPDA: 0.0
inactive: 0.0
fatty_acid: 0.0
Propionyl-CoA: 0.0
Methylmalonyl-CoA: 0.0
Methoxymalonyl-CoA: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0