BGC0000001 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000001 (Micromonospora maris AB-18-032)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS	1	59,320	High	abyssomicin C/atrop-abyssomicin C	PKS:Type I

BGC0000001 - Region 1 - T1PKS

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Location: 1 - 59,320 nt. (total: 59,320 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 59,321
Label		BGC0000001

Tailoring functions

Oxidoreductases: dehydrogenase oxygenase

abyV: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

abyX: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

orfU: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

Transferases: acylase

abyB2: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

Hydrolases: esterase phosphatase

abyA2: FkbH like protein

smcogs: SMCOG1256: FkbH like protein

abyT: thioesterase

smcogs: SMCOG1004: thioesterase

Lyases: cyclase

abyU: Diels-Alderase cyclase

extras: DA_cyclase: Diels-Alderase cyclase

Ligases: synthase

abyA1: 3-oxoacyl-(acyl carrier protein) synthase III

smcogs: SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III

abyB1: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

abyB3: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000001.5	1.00	PKS	abyssomicin C, atrop-abyssomicin C	Verrucosispora maris AB-18-032
BGC0001273.3	0.76	PKS	asperlactone	Aspergillus ochraceus
BGC0001276.3	0.76	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001275.3	0.76	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0000041.3	0.69	PKS	oronofacic acid	Pseudomonas syringae
BGC0002154.2	0.67	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001257.3	0.66	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0001258.3	0.66	PKS	1,3,6,8-tetrahydroxynaphthalene	Glarea lozoyensis
BGC0002191.2	0.66	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001284.4	0.66	PKS	alternariol	Parastagonospora nodorum SN15

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000001.5	1.00	PKS	abyssomicin C, atrop-abyssomicin C	Verrucosispora maris AB-18-032
BGC0001273.3	0.76	PKS	asperlactone	Aspergillus ochraceus
BGC0001276.3	0.76	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001275.3	0.76	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0000041.3	0.69	PKS	oronofacic acid	Pseudomonas syringae
BGC0002154.2	0.67	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001257.3	0.66	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0001258.3	0.66	PKS	1,3,6,8-tetrahydroxynaphthalene	Glarea lozoyensis
BGC0002191.2	0.66	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001284.4	0.66	PKS	alternariol	Parastagonospora nodorum SN15

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 59320:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (mal - ccmal - ccmal - ccmal) + (Me-ccmal - Me-redmal) + (mal)
Non-colinear ordering:: abyB1 + abyB2 + abyB3

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

abyB1: mal - mal - mal - mal

PKS_AT (510..791): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 75.0%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 194.2
inactive: 86.6
Methylmalonyl-CoA: 84.4
Methoxymalonyl-CoA: 82.1
Propionyl-CoA: 43.3
Ethylmalonyl-CoA: 37.1
Isobutyryl-CoA: 35.1
CHC-CoA: 34.7
2-Methylbutyryl-CoA: 26.2
Benzoyl-CoA: 25.6
Acetyl-CoA: 22.6
fatty_acid: 19.3
trans-1,2-CPDA: 15.2
3-Methylbutyryl-CoA: 5.9

PKS_AT (1477..1760): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 75.0%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 165.1
inactive: 77.3
Methoxymalonyl-CoA: 60.9
Methylmalonyl-CoA: 45.5
Propionyl-CoA: 22.9
Acetyl-CoA: 17.3
fatty_acid: 13.8
Ethylmalonyl-CoA: 11.9
2-Methylbutyryl-CoA: 9.5
CHC-CoA: 9.4
Benzoyl-CoA: 9.3
trans-1,2-CPDA: 0.0
Isobutyryl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (3061..3344): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 75.0%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 165.1
inactive: 77.3
Methoxymalonyl-CoA: 60.9
Methylmalonyl-CoA: 45.5
Propionyl-CoA: 22.9
Acetyl-CoA: 17.3
fatty_acid: 13.8
Ethylmalonyl-CoA: 11.9
2-Methylbutyryl-CoA: 9.5
CHC-CoA: 9.4
Benzoyl-CoA: 9.3
trans-1,2-CPDA: 0.0
Isobutyryl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (4661..4926): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 173.0
inactive: 82.7
Methoxymalonyl-CoA: 51.8
Methylmalonyl-CoA: 46.7
Propionyl-CoA: 23.7
Acetyl-CoA: 22.8
fatty_acid: 18.8
Isobutyryl-CoA: 16.3
Benzoyl-CoA: 14.7
CHC-CoA: 12.6
Ethylmalonyl-CoA: 9.1
trans-1,2-CPDA: 7.9
3-Methylbutyryl-CoA: 7.4
2-Methylbutyryl-CoA: 7.0

abyB2: mmal - mmal

PKS_AT (573..845): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 200.3
Ethylmalonyl-CoA: 188.7
Methoxymalonyl-CoA: 171.2
2-Methylbutyryl-CoA: 140.8
trans-1,2-CPDA: 121.1
Propionyl-CoA: 113.4
Malonyl-CoA: 106.1
CHC-CoA: 95.7
Isobutyryl-CoA: 77.7
Acetyl-CoA: 61.6
Benzoyl-CoA: 52.4
3-Methylbutyryl-CoA: 46.5
fatty_acid: 39.9
inactive: 38.6

PKS_AT (2160..2445): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 195.4
Ethylmalonyl-CoA: 181.3
Methoxymalonyl-CoA: 158.2
trans-1,2-CPDA: 123.8
Malonyl-CoA: 105.8
2-Methylbutyryl-CoA: 103.7
Propionyl-CoA: 98.5
CHC-CoA: 86.3
Isobutyryl-CoA: 41.0
Benzoyl-CoA: 38.8
Acetyl-CoA: 38.4
3-Methylbutyryl-CoA: 36.3
inactive: 30.5
fatty_acid: 29.3

abyB3: mal

PKS_AT (550..838): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 172.9
inactive: 94.0
Methoxymalonyl-CoA: 47.5
Methylmalonyl-CoA: 40.3
Isobutyryl-CoA: 32.1
Propionyl-CoA: 16.6
CHC-CoA: 9.2
Acetyl-CoA: 2.2
Benzoyl-CoA: 0.8
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: -1.5
Ethylmalonyl-CoA: -3.3

Link to PARAS substrate predictor