BGC0000014 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000014 (Sorangium cellulosum)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS,NRPS-like	1	100,708	High	ambruticin	PKS

BGC0000014 - Region 1 - NRPS-like,T1PKS

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Location: 1 - 100,708 nt. (total: 100,708 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX) and not alpha_am_amid)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 100,709
Label		BGC0000014

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase oxygenase

amb2: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

amb9: NADH:flavin oxidoreductase/NADH oxidase

smcogs: SMCOG1217: NADH:flavin oxidoreductase/NADH oxidase

ambB: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

ambD: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

ambJ: monooxygenase FAD-binding

smcogs: SMCOG1246: monooxygenase FAD-binding

ambN: aldehyde dehydrogenase

smcogs: SMCOG1017: aldehyde dehydrogenase

Transferases: aminotransferase methyltransferase

ambR: aminotransferase class-III

smcogs: SMCOG1013: aminotransferase class-III

ambS: methyltransferase

smcogs: SMCOG1089: methyltransferase

Ligases: ligase synthase

ambA: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

ambC: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

ambE: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

ambF: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

ambG: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

ambH: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000014.5	1.52	PKS	ambruticin	Sorangium cellulosum
BGC0001276.3	0.94	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001164.3	0.83	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001160.5	0.82	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001273.3	0.81	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	0.80	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.78	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001163.3	0.72	PKS	1-heptadecene	Moorea producens 3L
BGC0001257.3	0.67	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0002191.2	0.66	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000014.5	1.00	PKS	ambruticin	Sorangium cellulosum
BGC0001273.3	0.77	PKS	asperlactone	Aspergillus ochraceus
BGC0001276.3	0.77	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001275.3	0.76	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.76	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001160.5	0.73	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001164.3	0.73	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0000080.5	0.73	PKS	jerangolid A, jerangolid D	Sorangium cellulosum
BGC0001254.3	0.67	PKS	ACT-Toxin II	Alternaria alternata
BGC0001257.3	0.67	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 91724:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (NH2) + (Me-mal) + (Me-ohmal - redmal) + (Me-ccmal - ccmal) + (ccmal) + (Me-ccmal - ccmal) + (mal) + (pk)
Non-colinear ordering:: ambG + ambA + ambB + ambC + ambD + ambE + ambF + ambH

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ambA: mmal

PKS_AT (562..859): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 91.7%; Malonyl-CoA: 83.3%; Methoxymalonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 186.9
Ethylmalonyl-CoA: 177.1
Methoxymalonyl-CoA: 154.5
2-Methylbutyryl-CoA: 120.6
Isobutyryl-CoA: 103.5
Malonyl-CoA: 99.0
Propionyl-CoA: 98.2
trans-1,2-CPDA: 85.1
CHC-CoA: 76.0
Benzoyl-CoA: 56.5
fatty_acid: 52.2
Acetyl-CoA: 49.6
3-Methylbutyryl-CoA: 24.2
inactive: 19.5

ambB: mmal - mal

PKS_AT (570..867): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Malonyl-CoA: 87.5%; Methoxymalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 203.0
Ethylmalonyl-CoA: 191.7
Methoxymalonyl-CoA: 163.8
2-Methylbutyryl-CoA: 131.3
Malonyl-CoA: 109.9
Propionyl-CoA: 105.8
trans-1,2-CPDA: 98.7
Isobutyryl-CoA: 96.2
CHC-CoA: 86.8
Acetyl-CoA: 60.1
fatty_acid: 59.5
Benzoyl-CoA: 54.8
inactive: 29.5
3-Methylbutyryl-CoA: 29.1

PKS_AT (2056..2356): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 75.0%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 203.8
inactive: 116.0
Methoxymalonyl-CoA: 72.9
Methylmalonyl-CoA: 62.5
Isobutyryl-CoA: 41.3
Propionyl-CoA: 38.4
Benzoyl-CoA: 29.6
Ethylmalonyl-CoA: 26.6
2-Methylbutyryl-CoA: 26.3
Acetyl-CoA: 23.7
CHC-CoA: 11.7
trans-1,2-CPDA: 11.0
fatty_acid: 10.2
3-Methylbutyryl-CoA: 0.0

ambC: mmal - mal

PKS_AT (571..868): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Malonyl-CoA: 87.5%; Methoxymalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 205.8
Ethylmalonyl-CoA: 201.2
Methoxymalonyl-CoA: 171.1
2-Methylbutyryl-CoA: 132.1
Propionyl-CoA: 114.3
Isobutyryl-CoA: 113.1
Malonyl-CoA: 111.2
trans-1,2-CPDA: 101.6
CHC-CoA: 94.3
Benzoyl-CoA: 72.0
Acetyl-CoA: 69.8
fatty_acid: 50.2
inactive: 34.9
3-Methylbutyryl-CoA: 34.2

PKS_AT (2350..2651): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 75.0%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 203.8
inactive: 116.0
Methoxymalonyl-CoA: 72.9
Methylmalonyl-CoA: 62.5
Isobutyryl-CoA: 41.3
Propionyl-CoA: 38.4
Benzoyl-CoA: 29.6
Ethylmalonyl-CoA: 26.6
2-Methylbutyryl-CoA: 26.3
Acetyl-CoA: 23.7
CHC-CoA: 11.7
trans-1,2-CPDA: 11.0
fatty_acid: 10.2
3-Methylbutyryl-CoA: 0.0

ambD: mal

PKS_AT (580..881): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 216.8
inactive: 137.6
Methoxymalonyl-CoA: 72.6
Methylmalonyl-CoA: 60.9
Propionyl-CoA: 31.1
Isobutyryl-CoA: 28.3
Ethylmalonyl-CoA: 24.2
Acetyl-CoA: 23.9
trans-1,2-CPDA: 22.0
Benzoyl-CoA: 18.8
CHC-CoA: 15.5
2-Methylbutyryl-CoA: 12.7
fatty_acid: 10.3
3-Methylbutyryl-CoA: 0.0

ambE: mmal - mal

PKS_AT (570..865): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Malonyl-CoA: 87.5%; Methoxymalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 198.9
Ethylmalonyl-CoA: 190.3
Methoxymalonyl-CoA: 158.7
2-Methylbutyryl-CoA: 123.8
Malonyl-CoA: 105.7
Propionyl-CoA: 98.7
Isobutyryl-CoA: 97.0
trans-1,2-CPDA: 95.0
CHC-CoA: 83.1
Acetyl-CoA: 65.8
Benzoyl-CoA: 59.7
fatty_acid: 52.6
3-Methylbutyryl-CoA: 31.0
inactive: 29.5

PKS_AT (2342..2643): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 75.0%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 227.9
inactive: 121.6
Methoxymalonyl-CoA: 70.9
Methylmalonyl-CoA: 61.0
Isobutyryl-CoA: 32.4
Ethylmalonyl-CoA: 29.2
Propionyl-CoA: 29.0
Benzoyl-CoA: 27.0
Acetyl-CoA: 23.0
2-Methylbutyryl-CoA: 18.0
CHC-CoA: 14.4
trans-1,2-CPDA: 13.6
fatty_acid: 10.1
3-Methylbutyryl-CoA: 0.0

ambG: NH2

CAL_domain (53..497): NH2

minowa: NH2

Prediction, score:

NH2: 12.3
shikimic_acid: 11.8
AHBA: 7.7
Acetyl-CoA: 0.8
fatty_acid: 0.0

ambH: pk

PKS_AT (561..857): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 83.3%; Methylmalonyl-CoA: 66.7%; Propionyl-CoA: 62.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 135.0
Malonyl-CoA: 125.5
Methoxymalonyl-CoA: 115.5
Isobutyryl-CoA: 114.8
Ethylmalonyl-CoA: 83.6
Acetyl-CoA: 79.7
Propionyl-CoA: 79.5
trans-1,2-CPDA: 68.1
Benzoyl-CoA: 64.4
2-Methylbutyryl-CoA: 63.2
inactive: 57.4
CHC-CoA: 41.0
3-Methylbutyryl-CoA: 37.4
fatty_acid: 35.9

Link to PARAS substrate predictor