Genomic loci for this biosynthetic pathway

Cluster Type From To
The following clusters are from record BGC0000028.1:
Cluster 1Polyketide174009

BGC0000028, bafilomycin biosynthetic gene cluster from Streptomyces lohii. Locus 1. Full MIBiG entry.

Chemical compounds

Compound: Bafilomycin B1
SMILES string: Copy to clipboard
Molecular formula: C35H58O9
Exact molecular mass: 132.0655 Da ([M+H]+)
Molecular activity: Antibacterial, Antifungal, Other

Class-specific details

Biosynthetic class(es):
Polyketide
Polyketide subclass:
Macrolide (cyclic)
Polyketide synthase subclass:
Modular type I
Starter unit:
Isobutyryl-CoA
Thioesterase type:
Type II
Release / cyclization type:
Macrolactamization

Modular polyketide synthases:
BafAI
Module 0
AT specificity: Methylmalonyl-CoA
Evidence for specificity: Unknown
Module 1
AT specificity: Methylmalonyl-CoA
Evidence for specificity: Unknown
Module 2
AT specificity: Methylmalonyl-CoA
Evidence for specificity: Unknown
Module 3
AT specificity: Methylmalonyl-CoA
Evidence for specificity: Unknown

BafAII
Module 4
AT specificity: Methylmalonyl-CoA
Evidence for specificity: Unknown
Module 5
AT specificity: Methylmalonyl-CoA
Evidence for specificity: Unknown
Module 6
AT specificity: Methylmalonyl-CoA
Evidence for specificity: Unknown

BafAIII
Module 7
AT specificity: Methylmalonyl-CoA
Evidence for specificity: Unknown
Module 8
AT specificity: Methylmalonyl-CoA
Evidence for specificity: Unknown

BafAIV
Module 9
AT specificity: Methylmalonyl-CoA
Evidence for specificity: Unknown
Module 10
AT specificity: Methylmalonyl-CoA
Evidence for specificity: Unknown

BafV
Module 11
AT specificity: Methoxymalonyl-CoA
Evidence for specificity: Unknown

Gene cluster description

bafilomycin (BGC0000028). Gene Cluster 1. Biosynthetic class = Polyketide. GenBank GU390405, positions 1-74009. Click on genes for more information.

Legend:

biosynthetic genes
transport-related genes
regulatory genes
other genes

Domain annotation

Homologous known gene clusters

General MIBiG information on this cluster

Complete gene cluster sequence?complete
Evidence for cluster-compound connection:Knock-out studies, Enzymatic assays
MIxS-compliance:Unknown
Contact for this cluster:Fengan Yu (University of Michigan-Ann Arbor)

Literature references

1. Zhang W et al. (2013) Characterization of the bafilomycin biosynthetic gene cluster from Streptomyces lohii. Chembiochem 14(3):301-6. doi: 10.1002/cbic.201200743. Epub 2013 Jan