BGC0000028 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000028 (Streptomyces lohii)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	T1PKS	1	74,009	JBIR-100	Polyketide:Modular type I	94%

BGC0000028 - Region 1 - T1PKS

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Location: 1 - 74,009 nt. (total: 74,009 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

External annotation

Name		MIBiG
Version		3.0

Type		Subregion
Location		1 - 74,010
Label		BGC0000028

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000028.1	0.81	Polyketide	bafilomycin B1	Streptomyces lohii
BGC0000041.1	0.50	Polyketide	oronofacic acid	Pseudomonas syringae
BGC0000805.1	0.44	Saccharide	moenomycin	Streptomyces viridosporus ATCC 14672
BGC0001348.1	0.33	Polyketide	JBIR-100	Streptomyces varsoviensis
BGC0001164.1	0.29	Polyketide	1-heptadecene	Cyanothece sp. PCC 7822
BGC0000003.1	0.28	Polyketide	AF-toxin	Alternaria alternata
BGC0002071.1	0.27	NRP	Virginiafactin	Pseudomonas sp. QS1027
BGC0001544.1	0.26	NRP, Polyketide	chlorflavonin	Aspergillus campestris IBT 28561
BGC0001283.1	0.25	Polyketide	arsono-polyketide	Streptomyces lividans 1326
BGC0001302.1	0.25	Other	lomofungin	Streptomyces lomondensis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000028.1	0.94	Polyketide	bafilomycin B1	Streptomyces lohii
BGC0000041.1	0.78	Polyketide	oronofacic acid	Pseudomonas syringae
BGC0000805.1	0.75	Saccharide	moenomycin	Streptomyces viridosporus ATCC 14672
BGC0001348.1	0.66	Polyketide	JBIR-100	Streptomyces varsoviensis
BGC0001164.1	0.62	Polyketide	1-heptadecene	Cyanothece sp. PCC 7822
BGC0000003.1	0.61	Polyketide	AF-toxin	Alternaria alternata
BGC0002071.1	0.60	NRP	Virginiafactin	Pseudomonas sp. QS1027
BGC0001544.1	0.60	NRP, Polyketide	chlorflavonin	Aspergillus campestris IBT 28561
BGC0001283.1	0.58	Polyketide	arsono-polyketide	Streptomyces lividans 1326
BGC0001302.1	0.58	Other	lomofungin	Streptomyces lomondensis

Similar known gene clusters

Module view

Legend

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 74009:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (Me-mal - Me-ohmal - ohmal - Me-mal) + (pk - Me-redmal) + (Me-ohmal - ohemal - ccmal) + (Me-ohmal - Me-ccmal) + (Me-ccmal)
Non-colinear ordering:: ADC79616.1 + ADC79618.1 + ADC79617.1 + ADC79619.1 + ADC79620.1

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ADC79613.1

Search NORINE for peptide: strict or relaxed

AMP-binding (10..414): X

NRPSPredictor2: (unknown)

SVM prediction details:

NOTE: outside applicability domain

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: tFcmFGGpVK
Nearest Stachelhaus code:: leu
Stachelhaus code match:: 60% (weak)

ADC79614.1

Search NORINE for peptide: strict or relaxed

AMP-binding (15..418): X

NRPSPredictor2: (unknown)

SVM prediction details:

NOTE: outside applicability domain

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: shAlTvvfVK
Nearest Stachelhaus code:: leu
Stachelhaus code match:: 40% (weak)

ADC79616.1

PKS_AT (106..405): mmal

ATSignature: Isobutyryl-CoA

Top 3 matches:: Isobutyryl-CoA: 79.2%; Methoxymalonyl-CoA: 70.8%; Methylmalonyl-CoA: 70.8%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 161.1
Ethylmalonyl-CoA: 157.2
2-Methylbutyryl-CoA: 153.9
Methoxymalonyl-CoA: 134.5
Propionyl-CoA: 134.0
CHC-CoA: 94.5
trans-1,2-CPDA: 87.3
Malonyl-CoA: 84.7
Isobutyryl-CoA: 80.4
Benzoyl-CoA: 46.1
Acetyl-CoA: 34.9
3-Methylbutyryl-CoA: 30.8
inactive: 23.0
fatty_acid: 22.1

PKS_AT (1120..1414): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 211.9
Ethylmalonyl-CoA: 206.7
Methoxymalonyl-CoA: 168.8
2-Methylbutyryl-CoA: 162.1
trans-1,2-CPDA: 135.7
Propionyl-CoA: 119.4
CHC-CoA: 99.3
Malonyl-CoA: 87.5
Benzoyl-CoA: 81.7
Isobutyryl-CoA: 73.9
Acetyl-CoA: 55.4
fatty_acid: 41.1
3-Methylbutyryl-CoA: 32.0
inactive: 20.8

PKS_AT (2714..3015): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 235.9
inactive: 116.4
Methoxymalonyl-CoA: 72.9
Methylmalonyl-CoA: 60.1
Propionyl-CoA: 45.7
Isobutyryl-CoA: 36.7
Benzoyl-CoA: 21.5
Ethylmalonyl-CoA: 21.3
2-Methylbutyryl-CoA: 17.5
Acetyl-CoA: 17.1
CHC-CoA: 12.0
fatty_acid: 9.2
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (4311..4607): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Malonyl-CoA: 87.5%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 201.0
Ethylmalonyl-CoA: 198.4
Methoxymalonyl-CoA: 165.9
2-Methylbutyryl-CoA: 142.8
trans-1,2-CPDA: 122.0
Propionyl-CoA: 113.3
CHC-CoA: 91.3
Malonyl-CoA: 81.6
Isobutyryl-CoA: 75.6
Benzoyl-CoA: 69.2
Acetyl-CoA: 47.8
3-Methylbutyryl-CoA: 42.0
fatty_acid: 37.7
inactive: 29.7

ADC79617.1

PKS_AT (578..878): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 215.6
Ethylmalonyl-CoA: 205.1
Methoxymalonyl-CoA: 174.1
2-Methylbutyryl-CoA: 165.4
trans-1,2-CPDA: 119.5
Propionyl-CoA: 115.7
CHC-CoA: 106.6
Malonyl-CoA: 93.4
Isobutyryl-CoA: 80.9
Benzoyl-CoA: 79.6
Acetyl-CoA: 67.8
3-Methylbutyryl-CoA: 45.5
fatty_acid: 44.9
inactive: 28.4

PKS_AT (2166..2464): emal

ATSignature: Ethylmalonyl-CoA, Methylmalonyl-CoA, Methoxymalonyl-CoA

Top 3 matches:: Ethylmalonyl-CoA: 95.8%; Methylmalonyl-CoA: 95.8%; Methoxymalonyl-CoA: 95.8%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 222.7
Methylmalonyl-CoA: 202.7
Methoxymalonyl-CoA: 183.9
trans-1,2-CPDA: 144.9
2-Methylbutyryl-CoA: 137.2
Propionyl-CoA: 104.7
Malonyl-CoA: 97.4
CHC-CoA: 89.5
Benzoyl-CoA: 56.8
Isobutyryl-CoA: 55.8
Acetyl-CoA: 38.9
3-Methylbutyryl-CoA: 30.3
fatty_acid: 29.1
inactive: 27.0

PKS_AT (3812..4103): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 79.2%; 2-Methylbutyryl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 213.0
inactive: 106.4
Methoxymalonyl-CoA: 62.2
Methylmalonyl-CoA: 55.8
Propionyl-CoA: 35.8
Ethylmalonyl-CoA: 31.9
Isobutyryl-CoA: 24.1
Acetyl-CoA: 23.0
CHC-CoA: 19.1
2-Methylbutyryl-CoA: 18.5
Benzoyl-CoA: 10.8
fatty_acid: 6.2
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

ADC79618.1

PKS_AT (605..903): pk

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 232.8
Methylmalonyl-CoA: 228.1
Methoxymalonyl-CoA: 198.0
2-Methylbutyryl-CoA: 179.8
trans-1,2-CPDA: 149.0
Propionyl-CoA: 128.2
Malonyl-CoA: 121.3
CHC-CoA: 117.3
Benzoyl-CoA: 83.6
Isobutyryl-CoA: 79.8
Acetyl-CoA: 46.2
3-Methylbutyryl-CoA: 44.6
fatty_acid: 41.1
inactive: 33.1

PKS_AT (2376..2670): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 215.2
Ethylmalonyl-CoA: 208.9
Methoxymalonyl-CoA: 176.9
2-Methylbutyryl-CoA: 164.3
trans-1,2-CPDA: 128.4
Propionyl-CoA: 125.1
CHC-CoA: 101.3
Malonyl-CoA: 96.8
Isobutyryl-CoA: 94.9
Benzoyl-CoA: 78.5
Acetyl-CoA: 63.5
fatty_acid: 46.9
3-Methylbutyryl-CoA: 37.7
inactive: 28.1

ADC79619.1

PKS_AT (622..921): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 213.4
Ethylmalonyl-CoA: 213.0
Methoxymalonyl-CoA: 180.8
2-Methylbutyryl-CoA: 145.5
trans-1,2-CPDA: 116.9
Propionyl-CoA: 107.5
Malonyl-CoA: 105.6
CHC-CoA: 104.0
Isobutyryl-CoA: 81.4
Benzoyl-CoA: 66.0
3-Methylbutyryl-CoA: 57.9
Acetyl-CoA: 52.1
inactive: 39.0
fatty_acid: 32.4

PKS_AT (2210..2502): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 212.8
Ethylmalonyl-CoA: 201.7
Methoxymalonyl-CoA: 171.2
2-Methylbutyryl-CoA: 141.8
trans-1,2-CPDA: 135.0
Propionyl-CoA: 109.4
CHC-CoA: 97.1
Malonyl-CoA: 92.8
Isobutyryl-CoA: 83.3
Benzoyl-CoA: 77.6
Acetyl-CoA: 48.1
fatty_acid: 39.4
3-Methylbutyryl-CoA: 39.4
inactive: 31.0

ADC79620.1

PKS_AT (567..862): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Methoxymalonyl-CoA: 87.5%; Ethylmalonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 201.3
Ethylmalonyl-CoA: 183.7
Methoxymalonyl-CoA: 169.7
2-Methylbutyryl-CoA: 157.8
trans-1,2-CPDA: 129.1
Propionyl-CoA: 114.2
Malonyl-CoA: 102.8
CHC-CoA: 97.5
Isobutyryl-CoA: 71.2
Benzoyl-CoA: 64.5
fatty_acid: 60.3
Acetyl-CoA: 35.7
3-Methylbutyryl-CoA: 33.6
inactive: 27.3

ADC79629.1

PKS_AT (16..277): pk

ATSignature: (unknown)

No matches above 50%

minowa: Isobutyryl-CoA

Prediction, score:

Isobutyryl-CoA: 14.9
trans-1,2-CPDA: 0.0
inactive: 0.0
fatty_acid: 0.0
Propionyl-CoA: 0.0
Methylmalonyl-CoA: 0.0
Methoxymalonyl-CoA: 0.0
Malonyl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

ADC79630.1

Search NORINE for peptide: strict or relaxed

AMP-binding (70..282): X

NRPSPredictor2: (unknown)

SVM prediction details:

NOTE: outside applicability domain

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: tTl-rsTgiK
Nearest Stachelhaus code:: lys-b
Stachelhaus code match:: 30% (weak)