BGC0000053 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000053 (Unknown)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS	1	63,201	High	elaiophylin	PKS

BGC0000053 - Region 1 - T1PKS

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Location: 1 - 63,201 nt. (total: 63,201 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 63,202
Label		BGC0000053

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase

ctg1_orf20: aldo/keto reductase family oxidoreductase

smcogs: SMCOG1039: aldo/keto reductase family oxidoreductase

ctg1_orf22: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

ctg1_orf3: 3-hydroxybutyryl-CoA dehydrogenase

smcogs: SMCOG1095: 3-hydroxybutyryl-CoA dehydrogenase

ctg1_orf8: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Transferases: acylase glycosyltransferase transferase

ctg1_orf13: glycosyltransferase, MGT family

smcogs: SMCOG1062: glycosyltransferase, MGT family

ctg1_orf5: glucose-1-phosphate adenylyl/thymidylyltransferase

smcogs: SMCOG1064: glucose-1-phosphate adenylyl/thymidylyltransferase

ctg1_orf9: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

Hydrolases: esterase

ctg1_orf12: thioesterase

smcogs: SMCOG1004: thioesterase

Lyases: dehydratase

ctg1_orf19: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

ctg1_orf6: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

Ligases: synthase

ctg1_orf10: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

ctg1_orf11: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

ctg1_orf2: 3-oxoacyl-(acyl carrier protein) synthase III

smcogs: SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III

ctg1_orf7: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000053.5	0.98	PKS	elaiophylin	Unknown. Unclassified.
BGC0002291.3	0.84	PKS	efomycin K, efomycin L	Streptomyces sp. M56
BGC0001273.3	0.77	PKS	asperlactone	Aspergillus ochraceus
BGC0001244.3	0.76	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001275.3	0.75	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001276.3	0.75	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0000041.3	0.73	PKS	oronofacic acid	Pseudomonas syringae
BGC0002154.2	0.67	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001257.3	0.67	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0002191.2	0.66	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000053.5	0.98	PKS	elaiophylin	Unknown. Unclassified.
BGC0002291.3	0.84	PKS	efomycin K, efomycin L	Streptomyces sp. M56
BGC0001273.3	0.77	PKS	asperlactone	Aspergillus ochraceus
BGC0001244.3	0.76	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001275.3	0.75	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001276.3	0.75	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0000041.3	0.73	PKS	oronofacic acid	Pseudomonas syringae
BGC0002154.2	0.67	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001257.3	0.67	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0002191.2	0.66	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 63201:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (mal - ccemal - ohmal) + (Me-ohmal) + (pk) + (pk - ccmal) + (ccmal)
Non-colinear ordering:: ctg1_orf7 + ctg1_orf8 + ctg1_orf9 + ctg1_orf10 + ctg1_orf11

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg1_orf7: mal - emal - mal

PKS_AT (527..816): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 201.5
inactive: 115.3
Methoxymalonyl-CoA: 56.3
Methylmalonyl-CoA: 38.3
Propionyl-CoA: 23.8
Benzoyl-CoA: 22.1
Isobutyryl-CoA: 21.4
Acetyl-CoA: 20.6
fatty_acid: 15.4
Ethylmalonyl-CoA: 8.3
2-Methylbutyryl-CoA: 7.8
CHC-CoA: 3.9
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (1534..1832): emal

ATSignature: Ethylmalonyl-CoA

Top 3 matches:: Ethylmalonyl-CoA: 95.8%; Methylmalonyl-CoA: 91.7%; Methoxymalonyl-CoA: 91.7%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 210.6
Methylmalonyl-CoA: 191.1
Methoxymalonyl-CoA: 174.6
trans-1,2-CPDA: 140.0
2-Methylbutyryl-CoA: 135.0
CHC-CoA: 112.2
Malonyl-CoA: 108.7
Propionyl-CoA: 102.5
Isobutyryl-CoA: 75.4
Benzoyl-CoA: 72.4
3-Methylbutyryl-CoA: 45.7
Acetyl-CoA: 43.0
fatty_acid: 42.3
inactive: 18.9

PKS_AT (3294..3583): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 199.2
inactive: 111.7
Methoxymalonyl-CoA: 53.0
Methylmalonyl-CoA: 36.7
Isobutyryl-CoA: 22.4
Propionyl-CoA: 21.3
Acetyl-CoA: 20.2
Benzoyl-CoA: 19.6
fatty_acid: 13.4
Ethylmalonyl-CoA: 12.6
CHC-CoA: 7.2
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

ctg1_orf8: mmal

PKS_AT (576..871): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 217.5
Ethylmalonyl-CoA: 214.8
Methoxymalonyl-CoA: 177.2
2-Methylbutyryl-CoA: 160.4
trans-1,2-CPDA: 126.8
Propionyl-CoA: 122.8
CHC-CoA: 121.4
Malonyl-CoA: 103.9
Isobutyryl-CoA: 88.5
3-Methylbutyryl-CoA: 61.9
Benzoyl-CoA: 61.5
Acetyl-CoA: 55.1
fatty_acid: 46.8
inactive: 24.8

ctg1_orf9: pk

PKS_AT (568..860): pk

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 229.6
Methylmalonyl-CoA: 224.5
Methoxymalonyl-CoA: 181.7
2-Methylbutyryl-CoA: 164.1
trans-1,2-CPDA: 136.2
Propionyl-CoA: 118.4
CHC-CoA: 114.7
Malonyl-CoA: 103.1
Isobutyryl-CoA: 80.1
Benzoyl-CoA: 61.9
3-Methylbutyryl-CoA: 56.2
Acetyl-CoA: 46.6
fatty_acid: 37.1
inactive: 22.4

ctg1_orf10: pk - mal

PKS_AT (572..864): pk

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 229.5
Methylmalonyl-CoA: 224.4
Methoxymalonyl-CoA: 182.7
2-Methylbutyryl-CoA: 159.8
trans-1,2-CPDA: 136.5
Propionyl-CoA: 122.1
CHC-CoA: 108.9
Malonyl-CoA: 106.7
Isobutyryl-CoA: 74.6
Benzoyl-CoA: 58.6
3-Methylbutyryl-CoA: 52.1
Acetyl-CoA: 48.3
fatty_acid: 36.0
inactive: 24.7

PKS_AT (2101..2399): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 196.5
inactive: 109.7
Methoxymalonyl-CoA: 56.0
Methylmalonyl-CoA: 42.7
Propionyl-CoA: 28.5
Benzoyl-CoA: 23.2
Acetyl-CoA: 20.9
Isobutyryl-CoA: 17.4
Ethylmalonyl-CoA: 12.3
2-Methylbutyryl-CoA: 11.3
CHC-CoA: 10.3
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
3-Methylbutyryl-CoA: 0.0

ctg1_orf11: mal

PKS_AT (575..870): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 83.3%; inactive: 75.0%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 194.8
inactive: 97.5
Methoxymalonyl-CoA: 60.0
Methylmalonyl-CoA: 45.9
Propionyl-CoA: 31.3
Isobutyryl-CoA: 22.8
Acetyl-CoA: 21.7
Benzoyl-CoA: 21.6
Ethylmalonyl-CoA: 19.6
fatty_acid: 12.3
2-Methylbutyryl-CoA: 11.6
CHC-CoA: 11.5
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

Link to PARAS substrate predictor