BGC0000055: erythromycin A biosynthetic gene cluster from Saccharopolyspora erythraea NRRL 2338
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Location: 778,214 - 832,825 nt. (total: 54,612 nt).
This entry is originally from NCBI GenBank AM420293.1.

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core biosynthetic genes
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Gene details
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General
Compounds
Genes
Polyketide
Saccharide
History
NRPS/PKS domains
KnownClusterBlast
General information about the BGC
MIBiG accession BGC0000055
Short description erythromycin A biosynthetic gene cluster from Saccharopolyspora erythraea NRRL 2338
Status Minimal annotation: no
A minimal annotation only contains information on the BGC loci and one or more linked chemical product(s)

Completeness: complete
Whether the loci encodes everything needed for the pathway producing the compound(s)
Biosynthetic class(es)
  • Polyketide (Macrolide)
  • Saccharide (hybrid/tailoring)
Loci NCBI GenBank: AM420293.1
Compounds
  • erythromycin A
  • erythromycin B
  • erythromycin C
  • erythromycin D
Species Saccharopolyspora erythraea NRRL 2338 [taxonomy]
References
Chemical products information
erythromycin A [synonyms: abomacetin, erythromycin]
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C37H67N1O13
Chemical database entries
PubCHEM
ChEBI
ChEMBL
ChemSpider
erythromycin B [synonyms: berythromycin]
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C37H67N1O12
Chemical database entries
NPAtlas
PubCHEM
ChEBI
ChEMBL
ChemSpider
erythromycin C
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C36H65N1O13
Chemical database entries
NPAtlas
PubCHEM
ChEBI
ChemSpider
erythromycin D
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C36H65N1O12
Chemical database entries
PubCHEM
ChEBI
ChemSpider
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • SACE_0712
  • CAM00053.1
778214 - 778594 (+) putative erythromycin esterase
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  • SACE_0713
  • CAM00054.1
  • eryK
778654 - 779847 (+) cytochrome P450 Erythromycin B/D C-12 hydroxylase
  • Tailoring (Hydroxylation)
Activity assay
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  • SACE_0714
  • CAM00055.1
  • eryBVII
779802 - 780404 (-) dTDP-4-deoxyglucose 3,5-epimerase
  • Precursor biosynthesis
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  • SACE_0715
  • CAM00056.1
  • eryCV
780412 - 781881 (-) EryCV NDP-4,6-dideoxyhexose 3,4-enoyl reductase
  • Precursor biosynthesis
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  • SACE_0716
  • CAM00057.1
  • eryCIV
781919 - 783124 (-) eryCIV NDP-6-deoxyhexose 3,4-dehydratase
  • Precursor biosynthesis
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  • SACE_0717
  • CAM00058.1
  • eryBVI
783121 - 784584 (-) NDP-4-keto-6-deoxy-glucose 2,3-dehydratase
  • Precursor biosynthesis
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  • SACE_0718
  • CAM00059.1
  • eryCVI
784738 - 785451 (-) TDP-desosamine-N-dimethyltransferase
  • Precursor biosynthesis
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  • SACE_0719
  • CAM00060.1
  • eryBV
785504 - 786751 (-) 6-DEB TDP-mycarosyl glycosyltransferase
  • Tailoring (Glycosylation)
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  • SACE_0720
  • CAM00061.1
  • eryBIV
786751 - 787719 (-) dTDP-4-keto-6-deoxy-L-hexose 4-reductase
  • Precursor biosynthesis
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  • SACE_0721
  • CAM00062.1
  • eryAI
787944 - 798581 (+) EryAI Erythromycin polyketide synthase modules 1 and 2
  • Scaffold biosynthesis
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  • SACE_0722
  • CAM00063.1
798638 - 799849 (-) transposase
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  • SACE_0723
  • CAM00064.1
  • eryAII
800023 - 810726 (+) EryAII Erythromycin polyketide synthase modules 3 and 4
  • Scaffold biosynthesis
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  • SACE_0724
  • CAM00065.1
  • eryAIII
810727 - 820242 (+) EryAIII Erythromycin polyketide synthase modules 5 and 6
  • Scaffold biosynthesis
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  • SACE_0725
  • CAM00066.1
  • eryCII
820239 - 821324 (+) TDP-4-keto-6-deoxy-glucose 3,4-isomerase
  • Precursor biosynthesis
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  • SACE_0726
  • CAM00067.1
  • eryCIII
821335 - 822600 (+) glycosyl transferase, NDP-D-desosamine : 3-L-mycarosyl erythronolide B
  • Tailoring (Glycosylation)
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  • SACE_0727
  • CAM00068.1
  • eryBII
822597 - 823598 (+) TDP-4-keto-6-deoxyhexose 2,3-reductase
  • Precursor biosynthesis
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  • SACE_0728
  • CAM00069.1
  • eryG
823630 - 824550 (+) erythromycin C methlytransferase
  • Tailoring (Methylation)
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  • SACE_0729
  • CAM00070.1
824527 - 825270 (-) thioesterase, erythromycin biosynthesis
  • Activation / processing
Other in vivo study
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  • SACE_0730
  • CAM00071.1
  • eryF
825267 - 826481 (-) 6-deoxyerythronolide B hydroxylase (6-DEB hydroxylase)
  • Tailoring (Hydroxylation)
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  • SACE_0731
  • CAM00072.1
  • eryBIII
826478 - 827710 (-) eryBIII NDP-4-keto-2,6-dideoxyhexose 3-C-methyltransferase
  • Precursor biosynthesis
Other in vivo study
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  • SACE_0732
  • CAM00073.1
  • eryBI
827912 - 830338 (+) beta-D-glucosidase
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  • SACE_0733
  • CAM00074.1
  • ermE
830393 - 831538 (-) N-6-aminoadenine-N-methyltransferase, erythromycinresistance
  • Resistance/immunity
Other in vivo study
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  • SACE_0734
  • CAM00075.1
  • eryCI
831725 - 832825 (+) Erythromycin biosynthesis transaminase EryCI
  • Precursor biosynthesis
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Polyketide-specific information
Subclass Macrolide
Starter unit Propionyl-CoA
Cyclic? yes
Release type
  • Macrolactonization
Polyketide-synthases
Genes Properties Modules
eryAII
+
SACE_0729
+
eryAIII
+
eryAI
Synthase subclass: Modular type I
Thioesterases:
  • SACE_0729 (Unknown)
Module 0
Specificity: Propionyl-CoA
Evidence for specificity: Structure-based inference
Genes: eryAI
Core domains: Acyltransferase, Thiolation (ACP/PCP)
Module 1
Specificity: Methylmalonyl-CoA
Evidence for specificity: Structure-based inference
Genes: eryAI
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: D-OH
Module 2
Specificity: Methylmalonyl-CoA
Evidence for specificity: Structure-based inference
Genes: eryAI
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: L-OH
Module 3
Specificity: Methylmalonyl-CoA
Evidence for specificity: Structure-based inference
Genes: eryAII
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: Inactive
Module 4
Specificity: Methylmalonyl-CoA
Evidence for specificity: Structure-based inference
Genes: eryAII
Core domains: Ketosynthase, Acyltransferase, Dehydratase, Enoylreductase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: Unknown
Module 5
Specificity: Methylmalonyl-CoA
Evidence for specificity: Structure-based inference
Genes: eryAIII
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: L-OH
Module 6
Specificity: Methylmalonyl-CoA
Evidence for specificity: Structure-based inference
Genes: eryAIII
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Thiolation (ACP/PCP), Thioesterase
KR-domain stereochemistry: L-OH
Saccharide-specific information
Subclass hybrid/tailoring
Sugar-encoding genes
  • eryCII, eryCIV, eryCV, eryCVI
  • eryBII, eryBIV, eryBVI, eryBVII
Glycosyltransferases
eryCIII
Specificity: D-desosamine
Evidence:
  • Activity assay
eryBV
Specificity: L-mycarose
Evidence:
  • Activity assay
Annotation changelog
MIBiG version Submitter Notes
1.0
  • Hidden contributor (ID: AK53UXEU2X7JUZMX7VY3GZAO, no GDPR consent given).
  • Submitted
2.0
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Migrated from v1.4
  • Updated compound(s) information (NPAtlas curation)
3.0
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Removed ketoreductase stereochemistry annotation from modules without ketoreductases
3.1
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Update chemical activity to schema version 2.11
Detailed domain annotation
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