BGC0000086 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000086 (Streptomyces lasalocidi)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS	1	81,959	High	lasalocid	PKS

BGC0000086 - Region 1 - T1PKS

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Location: 1 - 81,959 nt. (total: 81,959 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 81,960
Label		BGC0000086

Tailoring functions

Oxidoreductases: dehydrogenase oxygenase

lsd10: 3-hydroxybutyryl-CoA dehydrogenase

smcogs: SMCOG1095: 3-hydroxybutyryl-CoA dehydrogenase

lsd18: monooxygenase FAD-binding

smcogs: SMCOG1246: monooxygenase FAD-binding

lsd9: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

Transferases: acylase

lsd13: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

lsd15: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

lsd16: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

lsd17: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

Hydrolases: esterase hydrolase

BAG85035.1: beta-lactamase

smcogs: SMCOG1053: beta-lactamase

lsd4: thioesterase

smcogs: SMCOG1004: thioesterase

Ligases: synthase

lsd11: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

lsd12: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

lsd14: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

lsd3: 3-oxoacyl-(acyl carrier protein) synthase III

smcogs: SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000086.5	1.00	PKS	lasalocid	Streptomyces lasaliensis
BGC0000087.5	0.82	PKS	lasalocid	Streptomyces lasaliensis
BGC0001273.3	0.77	PKS	asperlactone	Aspergillus ochraceus
BGC0001276.3	0.76	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001275.3	0.76	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.75	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0000041.3	0.67	PKS	oronofacic acid	Pseudomonas syringae
BGC0002154.2	0.66	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0002191.2	0.66	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001284.4	0.66	PKS	alternariol	Parastagonospora nodorum SN15

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000086.5	1.00	PKS	lasalocid	Streptomyces lasaliensis
BGC0000087.5	0.82	PKS	lasalocid	Streptomyces lasaliensis
BGC0001273.3	0.77	PKS	asperlactone	Aspergillus ochraceus
BGC0001276.3	0.76	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001275.3	0.76	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.75	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0000041.3	0.67	PKS	oronofacic acid	Pseudomonas syringae
BGC0002154.2	0.66	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0002191.2	0.66	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001284.4	0.66	PKS	alternariol	Parastagonospora nodorum SN15

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 81959:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (mal - pk - redmal) + (pk - Me-redmal - pk) + (Me-mal) + (Me-ohmal) + (redmal - ohmal) + (Me-ccmal) + (mal)
Non-colinear ordering:: lsd11 + lsd12 + lsd13 + lsd14 + lsd15 + lsd16 + lsd17

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

lsd11: mal - pk - mal

PKS_AT (443..742): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 87.5%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 232.3
inactive: 106.5
Methoxymalonyl-CoA: 83.3
Methylmalonyl-CoA: 66.9
Propionyl-CoA: 52.9
Isobutyryl-CoA: 43.2
Ethylmalonyl-CoA: 30.0
2-Methylbutyryl-CoA: 29.3
Benzoyl-CoA: 22.7
fatty_acid: 20.6
Acetyl-CoA: 20.3
CHC-CoA: 14.7
3-Methylbutyryl-CoA: 13.0
trans-1,2-CPDA: 0.0

PKS_AT (1489..1780): pk

ATSignature: Ethylmalonyl-CoA

Top 3 matches:: Ethylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 87.5%; Methylmalonyl-CoA: 79.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 184.0
Ethylmalonyl-CoA: 174.0
Methoxymalonyl-CoA: 148.4
2-Methylbutyryl-CoA: 119.6
trans-1,2-CPDA: 114.4
CHC-CoA: 104.0
Malonyl-CoA: 96.8
Propionyl-CoA: 90.6
Isobutyryl-CoA: 66.8
Benzoyl-CoA: 32.1
fatty_acid: 30.5
inactive: 27.7
Acetyl-CoA: 26.1
3-Methylbutyryl-CoA: 21.3

PKS_AT (3292..3578): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 189.8
inactive: 87.2
Methoxymalonyl-CoA: 49.0
Methylmalonyl-CoA: 34.4
Propionyl-CoA: 27.2
Acetyl-CoA: 17.7
fatty_acid: 17.5
Benzoyl-CoA: 14.7
trans-1,2-CPDA: 14.0
Isobutyryl-CoA: 12.3
2-Methylbutyryl-CoA: 9.4
CHC-CoA: 7.1
Ethylmalonyl-CoA: 6.0
3-Methylbutyryl-CoA: 0.0

lsd12: pk - mmal - pk

PKS_AT (526..817): pk

ATSignature: Ethylmalonyl-CoA

Top 3 matches:: Ethylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 87.5%; Methylmalonyl-CoA: 79.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 187.0
Ethylmalonyl-CoA: 175.0
Methoxymalonyl-CoA: 152.0
2-Methylbutyryl-CoA: 118.5
trans-1,2-CPDA: 116.8
CHC-CoA: 105.1
Malonyl-CoA: 97.8
Propionyl-CoA: 90.6
Isobutyryl-CoA: 64.8
Benzoyl-CoA: 34.7
fatty_acid: 30.8
inactive: 30.6
Acetyl-CoA: 27.0
3-Methylbutyryl-CoA: 26.9

PKS_AT (2283..2580): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 200.7
Ethylmalonyl-CoA: 186.2
Methoxymalonyl-CoA: 160.7
2-Methylbutyryl-CoA: 133.6
trans-1,2-CPDA: 124.5
CHC-CoA: 97.3
Propionyl-CoA: 95.5
Malonyl-CoA: 80.7
Isobutyryl-CoA: 58.1
Benzoyl-CoA: 45.4
3-Methylbutyryl-CoA: 23.5
Acetyl-CoA: 17.5
fatty_acid: 16.4
inactive: 13.9

PKS_AT (4349..4640): pk

ATSignature: Ethylmalonyl-CoA

Top 3 matches:: Ethylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 87.5%; Methylmalonyl-CoA: 79.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 186.2
Ethylmalonyl-CoA: 174.2
Methoxymalonyl-CoA: 151.1
2-Methylbutyryl-CoA: 123.7
trans-1,2-CPDA: 111.6
CHC-CoA: 106.7
Malonyl-CoA: 99.3
Propionyl-CoA: 90.6
Isobutyryl-CoA: 68.0
Benzoyl-CoA: 34.2
inactive: 32.8
fatty_acid: 31.2
Acetyl-CoA: 24.8
3-Methylbutyryl-CoA: 24.5

lsd13: mmal

PKS_AT (569..866): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 197.1
Ethylmalonyl-CoA: 183.6
Methoxymalonyl-CoA: 156.0
2-Methylbutyryl-CoA: 131.7
trans-1,2-CPDA: 122.6
Propionyl-CoA: 94.1
CHC-CoA: 93.9
Malonyl-CoA: 78.9
Isobutyryl-CoA: 58.2
Benzoyl-CoA: 44.5
3-Methylbutyryl-CoA: 22.0
Acetyl-CoA: 18.9
fatty_acid: 15.4
inactive: 12.3

lsd14: mmal

PKS_AT (567..864): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 193.8
Ethylmalonyl-CoA: 178.6
Methoxymalonyl-CoA: 153.7
2-Methylbutyryl-CoA: 130.4
trans-1,2-CPDA: 121.5
Propionyl-CoA: 93.8
CHC-CoA: 92.0
Malonyl-CoA: 79.4
Isobutyryl-CoA: 53.7
Benzoyl-CoA: 42.3
3-Methylbutyryl-CoA: 24.5
Acetyl-CoA: 22.5
fatty_acid: 16.4
inactive: 11.2

lsd15: mal - mal

PKS_AT (564..850): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 180.5
inactive: 81.5
Methoxymalonyl-CoA: 43.8
Methylmalonyl-CoA: 29.9
Propionyl-CoA: 22.4
Acetyl-CoA: 15.2
fatty_acid: 12.5
2-Methylbutyryl-CoA: 12.1
Benzoyl-CoA: 11.9
Isobutyryl-CoA: 11.7
trans-1,2-CPDA: 9.8
CHC-CoA: 0.5
Ethylmalonyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (2654..2939): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 180.2
inactive: 81.2
Methoxymalonyl-CoA: 42.4
Methylmalonyl-CoA: 30.7
Propionyl-CoA: 23.0
Acetyl-CoA: 15.2
fatty_acid: 12.5
2-Methylbutyryl-CoA: 12.1
CHC-CoA: 12.0
Benzoyl-CoA: 11.9
Isobutyryl-CoA: 11.7
trans-1,2-CPDA: 11.3
Ethylmalonyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

lsd16: mmal

PKS_AT (605..902): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 196.4
Ethylmalonyl-CoA: 182.0
Methoxymalonyl-CoA: 155.0
2-Methylbutyryl-CoA: 138.1
trans-1,2-CPDA: 121.6
Propionyl-CoA: 96.9
CHC-CoA: 93.7
Malonyl-CoA: 81.3
Isobutyryl-CoA: 55.4
Benzoyl-CoA: 50.2
3-Methylbutyryl-CoA: 25.4
fatty_acid: 21.6
Acetyl-CoA: 18.1
inactive: 14.7

lsd17: mal

PKS_AT (525..804): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 184.5
inactive: 82.7
Methoxymalonyl-CoA: 43.7
Methylmalonyl-CoA: 30.1
Propionyl-CoA: 23.1
Acetyl-CoA: 14.9
fatty_acid: 13.9
Benzoyl-CoA: 12.1
Isobutyryl-CoA: 12.0
trans-1,2-CPDA: 9.7
2-Methylbutyryl-CoA: 8.9
CHC-CoA: 6.0
Ethylmalonyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

Link to PARAS substrate predictor