BGC0000092 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000092 (Micromonospora megalomicea subsp. nigra)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS	1	47,981	High	megalomicin A/megalomicin B/megalomicin C1/megalomicin C2	PKS

BGC0000092 - Region 1 - T1PKS

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Location: 1 - 47,981 nt. (total: 47,981 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

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TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 47,982
Label		BGC0000092

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase oxygenase

megAII: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

megBII: aldo/keto reductase family oxidoreductase

smcogs: SMCOG1039: aldo/keto reductase family oxidoreductase

megCII: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

megDVI: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

megDVII: aldo/keto reductase family oxidoreductase

smcogs: SMCOG1039: aldo/keto reductase family oxidoreductase

megF: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

Transferases: acylase acyltransferase aminotransferase glycosyltransferase methyltransferase

megAI: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

megBV: glycosyltransferase, MGT family

smcogs: SMCOG1062: glycosyltransferase, MGT family

megCIII: glycosyltransferase, MGT family

smcogs: SMCOG1062: glycosyltransferase, MGT family

megDI: glycosyltransferase, MGT family

smcogs: SMCOG1062: glycosyltransferase, MGT family

megDII: DegT/DnrJ/EryC1/StrS aminotransferase

smcogs: SMCOG1056: DegT/DnrJ/EryC1/StrS aminotransferase

megDIII: methyltransferase

smcogs: SMCOG1248: methyltransferase

megY: acyltransferase 3

smcogs: SMCOG1088: acyltransferase 3

Hydrolases: esterase

megH: thioesterase

smcogs: SMCOG1004: thioesterase

Lyases: dehydratase

megBIV: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

megDV: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

Ligases: synthase

megAIII: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000092.5	1.00	PKS	megalomicin A, megalomicin B, megalomicin C1, megalomicin C2	Micromonospora megalomicea subsp. nigra
BGC0001817.5	0.67	other (other)	vancosamine	Streptomyces sp. WAC2288
BGC0001275.3	0.67	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0002192.3	0.64	PKS	FR901512	fungal sp. No.14919
BGC0000037.4	0.63	PKS	cichorine	Aspergillus nidulans FGSC A4
BGC0001160.5	0.63	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001164.3	0.63	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0000041.3	0.62	PKS	oronofacic acid	Pseudomonas syringae
BGC0001268.4	0.60	NRPS, PKS	fusarin C	Fusarium fujikuroi
BGC0000067.5	0.60	PKS	geldanamycin	Streptomyces hygroscopicus subsp. duamyceticus

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000092.5	1.00	PKS	megalomicin A, megalomicin B, megalomicin C1, megalomicin C2	Micromonospora megalomicea subsp. nigra
BGC0001817.5	0.67	other (other)	vancosamine	Streptomyces sp. WAC2288
BGC0001275.3	0.67	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0002192.3	0.64	PKS	FR901512	fungal sp. No.14919
BGC0000037.4	0.63	PKS	cichorine	Aspergillus nidulans FGSC A4
BGC0001160.5	0.63	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001164.3	0.63	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0000041.3	0.62	PKS	oronofacic acid	Pseudomonas syringae
BGC0001268.4	0.60	NRPS, PKS	fusarin C	Fusarium fujikuroi
BGC0000067.5	0.60	PKS	geldanamycin	Streptomyces hygroscopicus subsp. duamyceticus

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 47981:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (pk - Me-ohmal - Me-ohmal) + (Me-ohmal - Me-redmal) + (Me-ohmal - Me-ohmal)
Non-colinear ordering:: megAI + megAII + megAIII

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

megAI: pk - mmal - mmal

PKS_AT (110..404): pk

ATSignature: Propionyl-CoA

Top 3 matches:: Propionyl-CoA: 100.0%; 2-Methylbutyryl-CoA: 75.0%; Ethylmalonyl-CoA: 66.7%

minowa: Propionyl-CoA

Prediction, score:

Propionyl-CoA: 218.3
2-Methylbutyryl-CoA: 166.0
Methylmalonyl-CoA: 126.5
Ethylmalonyl-CoA: 117.4
Methoxymalonyl-CoA: 108.1
CHC-CoA: 85.5
trans-1,2-CPDA: 77.0
Malonyl-CoA: 70.4
Isobutyryl-CoA: 42.1
Acetyl-CoA: 41.5
fatty_acid: 29.0
Benzoyl-CoA: 25.9
3-Methylbutyryl-CoA: 20.1
inactive: 18.0

PKS_AT (1084..1384): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 215.0
Ethylmalonyl-CoA: 202.7
Methoxymalonyl-CoA: 180.2
2-Methylbutyryl-CoA: 143.8
trans-1,2-CPDA: 122.6
Propionyl-CoA: 121.7
Malonyl-CoA: 109.6
CHC-CoA: 94.3
Isobutyryl-CoA: 86.5
Benzoyl-CoA: 60.2
Acetyl-CoA: 45.6
fatty_acid: 44.8
3-Methylbutyryl-CoA: 42.1
inactive: 29.3

PKS_AT (2567..2864): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 219.5
Ethylmalonyl-CoA: 212.6
Methoxymalonyl-CoA: 177.0
2-Methylbutyryl-CoA: 142.3
trans-1,2-CPDA: 130.3
Propionyl-CoA: 117.0
Malonyl-CoA: 109.9
CHC-CoA: 98.1
Isobutyryl-CoA: 73.8
Benzoyl-CoA: 70.7
Acetyl-CoA: 60.7
inactive: 39.8
3-Methylbutyryl-CoA: 31.8
fatty_acid: 31.7

megAII: mmal - mmal

PKS_AT (561..859): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 214.7
Ethylmalonyl-CoA: 205.7
Methoxymalonyl-CoA: 179.9
2-Methylbutyryl-CoA: 130.0
trans-1,2-CPDA: 117.5
Malonyl-CoA: 110.5
Propionyl-CoA: 102.7
CHC-CoA: 86.2
Isobutyryl-CoA: 78.9
Benzoyl-CoA: 59.8
Acetyl-CoA: 45.1
3-Methylbutyryl-CoA: 39.8
fatty_acid: 32.0
inactive: 31.8

PKS_AT (2012..2309): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 210.4
Ethylmalonyl-CoA: 192.2
Methoxymalonyl-CoA: 170.6
2-Methylbutyryl-CoA: 135.7
trans-1,2-CPDA: 117.0
Propionyl-CoA: 110.3
Malonyl-CoA: 98.9
CHC-CoA: 88.1
Isobutyryl-CoA: 72.9
Benzoyl-CoA: 64.8
Acetyl-CoA: 54.7
fatty_acid: 37.3
inactive: 34.9
3-Methylbutyryl-CoA: 13.6

megAIII: mmal - mmal

PKS_AT (575..871): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 212.2
Ethylmalonyl-CoA: 197.2
Methoxymalonyl-CoA: 162.9
2-Methylbutyryl-CoA: 128.5
trans-1,2-CPDA: 113.8
Propionyl-CoA: 107.6
Malonyl-CoA: 102.0
CHC-CoA: 91.2
Isobutyryl-CoA: 82.4
Benzoyl-CoA: 69.4
Acetyl-CoA: 47.4
3-Methylbutyryl-CoA: 47.4
fatty_acid: 36.5
inactive: 33.7

PKS_AT (2045..2336): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 218.8
Ethylmalonyl-CoA: 208.2
Methoxymalonyl-CoA: 177.7
2-Methylbutyryl-CoA: 142.6
trans-1,2-CPDA: 122.2
Malonyl-CoA: 119.5
Propionyl-CoA: 103.4
CHC-CoA: 102.5
Isobutyryl-CoA: 75.5
Benzoyl-CoA: 73.6
Acetyl-CoA: 58.8
inactive: 46.0
3-Methylbutyryl-CoA: 35.8
fatty_acid: 35.5

Link to PARAS substrate predictor