BGC0000103 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000103 (Mycobacterium ulcerans Agy99)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS	1	147,218	High	mycolactone B	PKS

BGC0000103 - Region 1 - T1PKS

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Location: 1 - 147,218 nt. (total: 147,218 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 147,219
Label		BGC0000103

Tailoring functions

Ligases: synthase

MUP032c: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

MUP039c: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

MUP040c: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000103.5	1.00	PKS	mycolactone B	Mycobacterium ulcerans Agy99
BGC0001276.3	0.76	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001273.3	0.66	PKS	asperlactone	Aspergillus ochraceus
BGC0001244.3	0.66	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001275.3	0.66	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001284.4	0.66	PKS	alternariol	Parastagonospora nodorum SN15
BGC0001164.3	0.66	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001160.5	0.65	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0002192.3	0.63	PKS	FR901512	fungal sp. No.14919
BGC0001254.3	0.63	PKS	ACT-Toxin II	Alternaria alternata

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000103.5	1.00	PKS	mycolactone B	Mycobacterium ulcerans Agy99
BGC0001276.3	0.76	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001273.3	0.66	PKS	asperlactone	Aspergillus ochraceus
BGC0001244.3	0.66	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001275.3	0.66	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001284.4	0.66	PKS	alternariol	Parastagonospora nodorum SN15
BGC0001164.3	0.66	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001160.5	0.65	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0002192.3	0.63	PKS	FR901512	fungal sp. No.14919
BGC0001254.3	0.63	PKS	ACT-Toxin II	Alternaria alternata

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 147218:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (ccmal - ohmal - ohmal - Me-ccmal - ccmal - Me-ccmal - Me-ccmal - ccmal) + (ccmal - ccmal - Me-redmal - Me-ccmal - Me-redmal - ohmal - Me-ccmal - Me-redmal - ccmal) + (redmal)
Non-colinear ordering:: MUP032c + MUP040c + MUP039c

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

MUP032c: mal - mal - mal - mmal - mal - mmal - mmal - mal

PKS_AT (568..868): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 79.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 177.4
inactive: 95.1
Methoxymalonyl-CoA: 81.1
Methylmalonyl-CoA: 69.7
Propionyl-CoA: 54.7
CHC-CoA: 25.7
2-Methylbutyryl-CoA: 25.4
Ethylmalonyl-CoA: 24.3
trans-1,2-CPDA: 14.9
Isobutyryl-CoA: 14.9
Acetyl-CoA: 13.1
fatty_acid: 8.7
Benzoyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (2360..2649): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 184.7
inactive: 99.1
Methoxymalonyl-CoA: 73.7
Methylmalonyl-CoA: 60.0
Propionyl-CoA: 32.8
Ethylmalonyl-CoA: 30.7
fatty_acid: 27.8
Acetyl-CoA: 27.8
Benzoyl-CoA: 27.2
Isobutyryl-CoA: 24.4
2-Methylbutyryl-CoA: 22.8
CHC-CoA: 20.2
trans-1,2-CPDA: 18.4
3-Methylbutyryl-CoA: 0.0

PKS_AT (3899..4188): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 184.7
inactive: 99.1
Methoxymalonyl-CoA: 73.7
Methylmalonyl-CoA: 60.0
Propionyl-CoA: 32.8
Ethylmalonyl-CoA: 30.7
fatty_acid: 27.8
Acetyl-CoA: 27.8
Benzoyl-CoA: 27.2
Isobutyryl-CoA: 24.4
2-Methylbutyryl-CoA: 22.8
CHC-CoA: 20.2
trans-1,2-CPDA: 18.4
3-Methylbutyryl-CoA: 0.0

PKS_AT (5445..5745): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 87.5%; Ethylmalonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 200.6
Ethylmalonyl-CoA: 191.2
Methoxymalonyl-CoA: 172.8
2-Methylbutyryl-CoA: 134.1
trans-1,2-CPDA: 109.6
Malonyl-CoA: 105.4
Propionyl-CoA: 104.8
CHC-CoA: 92.9
Isobutyryl-CoA: 71.4
Acetyl-CoA: 66.1
3-Methylbutyryl-CoA: 57.6
Benzoyl-CoA: 48.8
inactive: 40.2
fatty_acid: 23.8

PKS_AT (7244..7544): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 79.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 177.4
inactive: 95.1
Methoxymalonyl-CoA: 81.1
Methylmalonyl-CoA: 69.7
Propionyl-CoA: 54.7
CHC-CoA: 25.7
2-Methylbutyryl-CoA: 25.4
Ethylmalonyl-CoA: 24.3
trans-1,2-CPDA: 14.9
Isobutyryl-CoA: 14.9
Acetyl-CoA: 13.1
fatty_acid: 8.7
Benzoyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (9043..9343): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 87.5%; Ethylmalonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 200.6
Ethylmalonyl-CoA: 191.2
Methoxymalonyl-CoA: 172.8
2-Methylbutyryl-CoA: 134.1
trans-1,2-CPDA: 109.6
Malonyl-CoA: 105.4
Propionyl-CoA: 104.8
CHC-CoA: 92.9
Isobutyryl-CoA: 71.4
Acetyl-CoA: 66.1
3-Methylbutyryl-CoA: 57.6
Benzoyl-CoA: 48.8
inactive: 40.2
fatty_acid: 23.8

PKS_AT (10842..11142): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 87.5%; Ethylmalonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 200.6
Ethylmalonyl-CoA: 191.2
Methoxymalonyl-CoA: 172.8
2-Methylbutyryl-CoA: 134.1
trans-1,2-CPDA: 109.6
Malonyl-CoA: 105.4
Propionyl-CoA: 104.8
CHC-CoA: 92.9
Isobutyryl-CoA: 71.4
Acetyl-CoA: 66.1
3-Methylbutyryl-CoA: 57.6
Benzoyl-CoA: 48.8
inactive: 40.2
fatty_acid: 23.8

PKS_AT (12641..12941): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 79.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 177.4
inactive: 95.1
Methoxymalonyl-CoA: 81.1
Methylmalonyl-CoA: 69.7
Propionyl-CoA: 54.7
CHC-CoA: 25.7
2-Methylbutyryl-CoA: 25.4
Ethylmalonyl-CoA: 24.3
trans-1,2-CPDA: 14.9
Isobutyryl-CoA: 14.9
Acetyl-CoA: 13.1
fatty_acid: 8.7
Benzoyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

MUP039c: mal

PKS_AT (591..891): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 79.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 177.4
inactive: 95.1
Methoxymalonyl-CoA: 81.1
Methylmalonyl-CoA: 69.7
Propionyl-CoA: 54.7
CHC-CoA: 25.7
2-Methylbutyryl-CoA: 25.4
Ethylmalonyl-CoA: 24.3
trans-1,2-CPDA: 14.9
Isobutyryl-CoA: 14.9
Acetyl-CoA: 13.1
fatty_acid: 8.7
Benzoyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

MUP040c: mal - mal - mmal - mmal - mmal - mal - mmal - mmal - mal

PKS_AT (568..868): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 79.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 177.4
inactive: 95.1
Methoxymalonyl-CoA: 81.1
Methylmalonyl-CoA: 69.7
Propionyl-CoA: 54.7
CHC-CoA: 25.7
2-Methylbutyryl-CoA: 25.4
Ethylmalonyl-CoA: 24.3
trans-1,2-CPDA: 14.9
Isobutyryl-CoA: 14.9
Acetyl-CoA: 13.1
fatty_acid: 8.7
Benzoyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (2373..2673): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 79.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 177.4
inactive: 95.1
Methoxymalonyl-CoA: 81.1
Methylmalonyl-CoA: 69.7
Propionyl-CoA: 54.7
CHC-CoA: 25.7
2-Methylbutyryl-CoA: 25.4
Ethylmalonyl-CoA: 24.3
trans-1,2-CPDA: 14.9
Isobutyryl-CoA: 14.9
Acetyl-CoA: 13.1
fatty_acid: 8.7
Benzoyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (4178..4478): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 87.5%; Ethylmalonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 200.6
Ethylmalonyl-CoA: 191.2
Methoxymalonyl-CoA: 172.8
2-Methylbutyryl-CoA: 134.1
trans-1,2-CPDA: 109.6
Malonyl-CoA: 105.4
Propionyl-CoA: 104.8
CHC-CoA: 92.9
Isobutyryl-CoA: 71.4
Acetyl-CoA: 66.1
3-Methylbutyryl-CoA: 57.6
Benzoyl-CoA: 48.8
inactive: 40.2
fatty_acid: 23.8

PKS_AT (6299..6599): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 87.5%; Ethylmalonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 200.6
Ethylmalonyl-CoA: 191.2
Methoxymalonyl-CoA: 172.8
2-Methylbutyryl-CoA: 134.1
trans-1,2-CPDA: 109.6
Malonyl-CoA: 105.4
Propionyl-CoA: 104.8
CHC-CoA: 92.9
Isobutyryl-CoA: 71.4
Acetyl-CoA: 66.1
3-Methylbutyryl-CoA: 57.6
Benzoyl-CoA: 48.8
inactive: 40.2
fatty_acid: 23.8

PKS_AT (8097..8397): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 87.5%; Ethylmalonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 200.6
Ethylmalonyl-CoA: 191.2
Methoxymalonyl-CoA: 172.8
2-Methylbutyryl-CoA: 134.1
trans-1,2-CPDA: 109.6
Malonyl-CoA: 105.4
Propionyl-CoA: 104.8
CHC-CoA: 92.9
Isobutyryl-CoA: 71.4
Acetyl-CoA: 66.1
3-Methylbutyryl-CoA: 57.6
Benzoyl-CoA: 48.8
inactive: 40.2
fatty_acid: 23.8

PKS_AT (10205..10494): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 184.7
inactive: 99.1
Methoxymalonyl-CoA: 73.7
Methylmalonyl-CoA: 60.0
Propionyl-CoA: 32.8
Ethylmalonyl-CoA: 30.7
fatty_acid: 27.8
Acetyl-CoA: 27.8
Benzoyl-CoA: 27.2
Isobutyryl-CoA: 24.4
2-Methylbutyryl-CoA: 22.8
CHC-CoA: 20.2
trans-1,2-CPDA: 18.4
3-Methylbutyryl-CoA: 0.0

PKS_AT (11751..12051): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 87.5%; Ethylmalonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 200.6
Ethylmalonyl-CoA: 191.2
Methoxymalonyl-CoA: 172.8
2-Methylbutyryl-CoA: 134.1
trans-1,2-CPDA: 109.6
Malonyl-CoA: 105.4
Propionyl-CoA: 104.8
CHC-CoA: 92.9
Isobutyryl-CoA: 71.4
Acetyl-CoA: 66.1
3-Methylbutyryl-CoA: 57.6
Benzoyl-CoA: 48.8
inactive: 40.2
fatty_acid: 23.8

PKS_AT (13544..13844): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 87.5%; Ethylmalonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 200.6
Ethylmalonyl-CoA: 191.2
Methoxymalonyl-CoA: 172.8
2-Methylbutyryl-CoA: 134.1
trans-1,2-CPDA: 109.6
Malonyl-CoA: 105.4
Propionyl-CoA: 104.8
CHC-CoA: 92.9
Isobutyryl-CoA: 71.4
Acetyl-CoA: 66.1
3-Methylbutyryl-CoA: 57.6
Benzoyl-CoA: 48.8
inactive: 40.2
fatty_acid: 23.8

PKS_AT (15664..15964): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 79.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 177.4
inactive: 95.1
Methoxymalonyl-CoA: 81.1
Methylmalonyl-CoA: 69.7
Propionyl-CoA: 54.7
CHC-CoA: 25.7
2-Methylbutyryl-CoA: 25.4
Ethylmalonyl-CoA: 24.3
trans-1,2-CPDA: 14.9
Isobutyryl-CoA: 14.9
Acetyl-CoA: 13.1
fatty_acid: 8.7
Benzoyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

Link to PARAS substrate predictor