BGC0000125 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000125 (Streptomyces natalensis)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS,NRPS-like	1	84,985	High	pimaricin	PKS

BGC0000125 - Region 1 - NRPS-like,T1PKS

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Location: 1 - 84,985 nt. (total: 84,985 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX) and not alpha_am_amid)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 84,986
Label		BGC0000125

Tailoring functions

Oxidoreductases: oxygenase

pimD: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

pimG: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

Transferases: aminotransferase glycosyltransferase

pimC: DegT/DnrJ/EryC1/StrS aminotransferase

smcogs: SMCOG1056: DegT/DnrJ/EryC1/StrS aminotransferase

pimK: glycosyltransferase, MGT family

smcogs: SMCOG1062: glycosyltransferase, MGT family

Hydrolases: esterase

pimI: thioesterase

smcogs: SMCOG1004: thioesterase

Lyases: dehydratase

pimJ: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

Ligases: synthase

pimS0: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

pimS1: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

pimS2: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

pimS3: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

pimS4: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000125.5	1.36	PKS	pimaricin	Streptomyces natalensis
BGC0001690.5	1.12	PKS	natamycin	Streptomyces gilvosporeus
BGC0001273.3	0.77	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	0.76	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001257.3	0.66	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0002154.2	0.66	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001284.4	0.66	PKS	alternariol	Parastagonospora nodorum SN15
BGC0001258.3	0.65	PKS	1,3,6,8-tetrahydroxynaphthalene	Glarea lozoyensis
BGC0002191.2	0.63	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0000138.5	0.60	PKS	rimocidin	Streptomyces diastaticus

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000125.5	1.00	PKS	pimaricin	Streptomyces natalensis
BGC0001690.5	0.94	PKS	natamycin	Streptomyces gilvosporeus
BGC0001273.3	0.76	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	0.76	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001276.3	0.75	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001244.3	0.75	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001257.3	0.67	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0002154.2	0.66	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0002175.3	0.66	PKS	YWA1	Aspergillus oryzae RIB40
BGC0002191.2	0.66	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 84985:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (ohmal - ccmal - ccmal - ccmal) + (X - mal) + (ccmal - ohmal - Me-ohmal - ohmal - ohmal - ohmal) + (ccmal) + (ccmal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

pimS4: mal

PKS_AT (550..835): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 229.7
inactive: 105.0
Methoxymalonyl-CoA: 59.9
Methylmalonyl-CoA: 56.0
Propionyl-CoA: 28.2
Acetyl-CoA: 25.0
Isobutyryl-CoA: 23.9
Benzoyl-CoA: 20.8
Ethylmalonyl-CoA: 15.4
CHC-CoA: 8.5
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

pimS3: mal

PKS_AT (551..836): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 229.0
inactive: 104.2
Methoxymalonyl-CoA: 62.2
Methylmalonyl-CoA: 54.3
Propionyl-CoA: 31.4
Isobutyryl-CoA: 26.7
Acetyl-CoA: 23.6
Ethylmalonyl-CoA: 17.9
Benzoyl-CoA: 17.3
2-Methylbutyryl-CoA: 13.9
fatty_acid: 7.0
CHC-CoA: 5.4
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

pimS2: mal - mal - mmal - mal - mal - mal

PKS_AT (561..851): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 79.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 208.7
inactive: 88.5
Methoxymalonyl-CoA: 56.7
Methylmalonyl-CoA: 47.2
Acetyl-CoA: 27.7
Propionyl-CoA: 27.3
Benzoyl-CoA: 23.6
Ethylmalonyl-CoA: 19.2
Isobutyryl-CoA: 18.8
2-Methylbutyryl-CoA: 18.7
CHC-CoA: 16.6
trans-1,2-CPDA: 10.1
fatty_acid: 9.3
3-Methylbutyryl-CoA: 0.0

PKS_AT (2317..2616): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 70.8%; Propionyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 199.6
inactive: 85.8
Methoxymalonyl-CoA: 70.3
Methylmalonyl-CoA: 60.0
Isobutyryl-CoA: 33.6
Propionyl-CoA: 29.9
Ethylmalonyl-CoA: 22.6
CHC-CoA: 22.1
Acetyl-CoA: 21.2
Benzoyl-CoA: 19.3
fatty_acid: 13.1
2-Methylbutyryl-CoA: 11.2
trans-1,2-CPDA: 9.3
3-Methylbutyryl-CoA: 0.0

PKS_AT (3860..4157): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 209.6
Ethylmalonyl-CoA: 193.4
Methoxymalonyl-CoA: 165.6
2-Methylbutyryl-CoA: 128.4
trans-1,2-CPDA: 126.0
Propionyl-CoA: 105.8
CHC-CoA: 94.8
Malonyl-CoA: 91.5
Isobutyryl-CoA: 73.1
Benzoyl-CoA: 62.1
Acetyl-CoA: 34.7
fatty_acid: 28.8
3-Methylbutyryl-CoA: 26.6
inactive: 17.4

PKS_AT (5393..5693): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 75.0%; Propionyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 216.8
inactive: 98.9
Methoxymalonyl-CoA: 72.9
Methylmalonyl-CoA: 61.0
Isobutyryl-CoA: 44.3
Propionyl-CoA: 30.0
Benzoyl-CoA: 19.9
Ethylmalonyl-CoA: 17.4
2-Methylbutyryl-CoA: 17.3
Acetyl-CoA: 15.8
fatty_acid: 13.5
3-Methylbutyryl-CoA: 10.5
CHC-CoA: 8.8
trans-1,2-CPDA: 0.0

PKS_AT (6913..7198): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 223.8
inactive: 100.2
Methoxymalonyl-CoA: 56.7
Methylmalonyl-CoA: 50.6
Isobutyryl-CoA: 27.9
Propionyl-CoA: 23.0
Acetyl-CoA: 22.7
Benzoyl-CoA: 21.3
Ethylmalonyl-CoA: 14.6
fatty_acid: 8.3
trans-1,2-CPDA: 0.0
CHC-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

PKS_AT (8430..8723): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 226.6
inactive: 106.3
Methoxymalonyl-CoA: 62.1
Methylmalonyl-CoA: 60.0
Isobutyryl-CoA: 38.2
Propionyl-CoA: 29.9
Benzoyl-CoA: 23.1
Ethylmalonyl-CoA: 22.5
Acetyl-CoA: 20.6
2-Methylbutyryl-CoA: 14.6
CHC-CoA: 11.2
fatty_acid: 10.7
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

pimS0: X - mal

AMP-binding (29..446): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LFQFRAVTGK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

PKS_AT (1209..1511): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 70.8%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 182.5
inactive: 72.9
Methoxymalonyl-CoA: 70.4
Methylmalonyl-CoA: 60.7
Isobutyryl-CoA: 35.1
Ethylmalonyl-CoA: 33.6
Propionyl-CoA: 32.9
Acetyl-CoA: 29.9
fatty_acid: 25.2
Benzoyl-CoA: 24.0
CHC-CoA: 23.1
2-Methylbutyryl-CoA: 15.3
trans-1,2-CPDA: 15.1
3-Methylbutyryl-CoA: 0.0

pimS1: mal - mal - mal - mal

PKS_AT (565..864): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 211.1
inactive: 101.9
Methoxymalonyl-CoA: 63.8
Methylmalonyl-CoA: 54.0
Isobutyryl-CoA: 46.0
Propionyl-CoA: 31.7
Benzoyl-CoA: 22.7
Acetyl-CoA: 19.9
Ethylmalonyl-CoA: 19.3
2-Methylbutyryl-CoA: 14.8
trans-1,2-CPDA: 12.6
CHC-CoA: 7.3
3-Methylbutyryl-CoA: 6.9
fatty_acid: 0.0

PKS_AT (2103..2393): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 213.5
inactive: 101.6
Methoxymalonyl-CoA: 51.1
Methylmalonyl-CoA: 42.0
Acetyl-CoA: 19.8
Propionyl-CoA: 18.9
Benzoyl-CoA: 15.5
2-Methylbutyryl-CoA: 11.5
fatty_acid: 7.7
CHC-CoA: 5.1
Isobutyryl-CoA: 4.5
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0
Ethylmalonyl-CoA: -3.9

PKS_AT (3829..4119): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 87.5%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 228.7
inactive: 108.0
Methoxymalonyl-CoA: 51.3
Methylmalonyl-CoA: 38.8
Propionyl-CoA: 28.9
Acetyl-CoA: 22.0
Benzoyl-CoA: 16.2
Ethylmalonyl-CoA: 12.9
fatty_acid: 11.7
Isobutyryl-CoA: 11.0
trans-1,2-CPDA: 0.0
CHC-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: -2.6

PKS_AT (5543..5833): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 227.8
inactive: 108.1
Methoxymalonyl-CoA: 63.1
Methylmalonyl-CoA: 47.4
Propionyl-CoA: 27.6
Acetyl-CoA: 24.7
Ethylmalonyl-CoA: 21.0
2-Methylbutyryl-CoA: 18.1
Benzoyl-CoA: 17.6
fatty_acid: 17.4
Isobutyryl-CoA: 13.1
CHC-CoA: 10.4
trans-1,2-CPDA: 6.9
3-Methylbutyryl-CoA: 0.0

Link to PARAS substrate predictor