BGC0000128 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000128 (Pseudomonas sp. M18)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS	1	32,800	High	pyoluteorin	PKS

BGC0000128 - Region 1 - T1PKS

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Location: 1 - 32,800 nt. (total: 32,800 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 32,801
Label		BGC0000128

Tailoring functions

Oxidoreductases: dehydrogenase

pltE: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

Transferases: acylase halogenase

pltA: flavin-dependent pyrrole halogenase

halogenases: flavin-dependent pyrrole halogenase
smcogs: SMCOG1119: halogenase

pltB: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

pltD: ambiguous flavin-dependent halogenase

halogenases: ambiguous flavin-dependent halogenase
smcogs: SMCOG1119: halogenase

pltM: ambiguous flavin-dependent halogenase

halogenases: ambiguous flavin-dependent halogenase
smcogs: SMCOG1119: halogenase

Hydrolases: esterase

pltG: thioesterase

smcogs: SMCOG1004: thioesterase

Ligases: ligase synthase

pltC: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

pltF: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000128.3	0.99	PKS	pyoluteorin	Pseudomonas sp. M18
BGC0002341.2	0.75	other (other)	3,5-dibromo-p-anisic acid	Planctomycetales bacterium 10988
BGC0000127.5	0.74	PKS	pyoluteorin	Pseudomonas protegens Pf-5
BGC0001244.3	0.67	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001276.3	0.67	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001273.3	0.67	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	0.66	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0002191.2	0.66	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001257.3	0.66	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0002154.2	0.66	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000128.3	0.99	PKS	pyoluteorin	Pseudomonas sp. M18
BGC0002341.2	0.75	other (other)	3,5-dibromo-p-anisic acid	Planctomycetales bacterium 10988
BGC0000127.5	0.74	PKS	pyoluteorin	Pseudomonas protegens Pf-5
BGC0001244.3	0.67	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001276.3	0.67	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001273.3	0.67	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	0.66	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0002191.2	0.66	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001257.3	0.66	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0002154.2	0.66	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 32800:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (mal) + (ohmal)
Non-colinear ordering:: pltB + pltC

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

pltB: mal

PKS_AT (534..826): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; 2-Methylbutyryl-CoA: 66.7%; Methylmalonyl-CoA: 66.7%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 121.7
Methylmalonyl-CoA: 104.7
Methoxymalonyl-CoA: 87.9
Isobutyryl-CoA: 71.3
2-Methylbutyryl-CoA: 66.7
Ethylmalonyl-CoA: 63.1
Propionyl-CoA: 55.0
CHC-CoA: 46.0
fatty_acid: 44.4
trans-1,2-CPDA: 39.0
inactive: 36.2
Benzoyl-CoA: 30.9
Acetyl-CoA: 25.9
3-Methylbutyryl-CoA: 14.5

pltC: mal

PKS_AT (570..868): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 176.6
inactive: 100.1
Methylmalonyl-CoA: 65.1
Methoxymalonyl-CoA: 63.7
Propionyl-CoA: 45.6
2-Methylbutyryl-CoA: 44.4
Isobutyryl-CoA: 32.6
CHC-CoA: 29.4
trans-1,2-CPDA: 24.5
Ethylmalonyl-CoA: 23.9
Benzoyl-CoA: 19.2
fatty_acid: 18.3
Acetyl-CoA: 16.3
3-Methylbutyryl-CoA: 15.5

pltF: Pro

Search NORINE for peptide: strict or relaxed

AMP-binding (9..403): Pro

nrpys: Pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DLLYLALVCK
Nearest Stachelhaus code(s):: Pro DLLYLALVCK (88% 8Å match)
Stachelhaus code match:: 100% (strong)

Link to PARAS substrate predictor