BGC0000130 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000130 (Wenjunlia vitaminophila)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS	1	55,655	High	pyrrolomycin A/pyrrolomycin B/pyrrolomycin C/pyrrolomycin D	PKS

BGC0000130 - Region 1 - T1PKS

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Location: 1 - 55,655 nt. (total: 55,655 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 55,656
Label		BGC0000130

Tailoring functions

Oxidoreductases: dehydrogenase electron transfer oxidoreductase oxygenase

ABO15870.1: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

pyr13: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

pyr15: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

pyr18: flavodoxin

smcogs: SMCOG1134: flavodoxin

pyr19: pyridine nucleotide-disulfide oxidoreductase

smcogs: SMCOG1175: pyridine nucleotide-disulfide oxidoreductase

pyr2: monooxygenase FAD-binding

smcogs: SMCOG1050: monooxygenase FAD-binding

pyr20: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

pyr31: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

pyr7: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

pyr9: flavin reductase domain protein FMN-binding

smcogs: SMCOG1160: flavin reductase domain protein FMN-binding

Transferases: PPTase halogenase methyltransferase

pyr11: ambiguous flavin-dependent halogenase

halogenases: ambiguous flavin-dependent halogenase
smcogs: SMCOG1119: halogenase

pyr16: conventional flavin-dependent halogenase

halogenases: conventional flavin-dependent halogenase
smcogs: SMCOG1119: halogenase

pyr17: conventional flavin-dependent halogenase

halogenases: conventional flavin-dependent halogenase
smcogs: SMCOG1119: halogenase

pyr22: methyltransferase

smcogs: SMCOG1089: methyltransferase

pyr29: flavin-dependent pyrrole halogenase

halogenases: flavin-dependent pyrrole halogenase
smcogs: SMCOG1119: halogenase

pyr30: 4'-phosphopantetheinyl transferase

smcogs: SMCOG1012: 4'-phosphopantetheinyl transferase

Hydrolases: esterase

pyr26: thioesterase

smcogs: SMCOG1004: thioesterase

Lyases: dehydratase

ABO15871.1: L-carnitine dehydratase/bile acid-inducible

smcogs: SMCOG1189: L-carnitine dehydratase/bile acid-inducible

Ligases: ligase synthase

pyr24: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

pyr25: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

pyr8: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000130.5	0.95	PKS	pyrrolomycin A, pyrrolomycin B, pyrrolomycin C, pyrrolomycin D	Streptomyces vitaminophilus
BGC0002341.2	0.75	other (other)	3,5-dibromo-p-anisic acid	Planctomycetales bacterium 10988
BGC0001276.3	0.67	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001273.3	0.67	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	0.67	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.67	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001257.3	0.66	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0000107.3	0.66	PKS	naphthopyrone	Aspergillus nidulans FGSC A4
BGC0001284.4	0.66	PKS	alternariol	Parastagonospora nodorum SN15
BGC0002154.2	0.66	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000130.5	1.00	PKS	pyrrolomycin A, pyrrolomycin B, pyrrolomycin C, pyrrolomycin D	Streptomyces vitaminophilus
BGC0000131.5	0.82	PKS	pyrrolomycin	Streptomyces sp. UC 11065
BGC0002341.2	0.75	other (other)	3,5-dibromo-p-anisic acid	Planctomycetales bacterium 10988
BGC0001276.3	0.67	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001273.3	0.67	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	0.67	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.67	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001257.3	0.66	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0000107.3	0.66	PKS	naphthopyrone	Aspergillus nidulans FGSC A4
BGC0001284.4	0.66	PKS	alternariol	Parastagonospora nodorum SN15

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 10892 - 55655:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (mal) + (ccmal)
Non-colinear ordering:: pyr25 + pyr24

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

pyr8: X

AMP-binding (6..373): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DLFYIAWVCK
Nearest Stachelhaus code(s):: Pro DLFYLAWVCK (94% 8Å match)
Stachelhaus code match:: 90% (moderate)

pyr24: mal

PKS_AT (572..866): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 87.5%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 199.4
inactive: 115.0
Methoxymalonyl-CoA: 74.5
Methylmalonyl-CoA: 74.4
Propionyl-CoA: 48.9
Isobutyryl-CoA: 48.1
Ethylmalonyl-CoA: 38.1
CHC-CoA: 27.2
2-Methylbutyryl-CoA: 26.2
Benzoyl-CoA: 24.3
Acetyl-CoA: 18.1
fatty_acid: 12.5
trans-1,2-CPDA: 12.0
3-Methylbutyryl-CoA: 0.0

pyr25: mal

PKS_AT (563..858): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; Methylmalonyl-CoA: 79.2%; Ethylmalonyl-CoA: 66.7%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 132.4
Methylmalonyl-CoA: 131.3
Methoxymalonyl-CoA: 105.5
Ethylmalonyl-CoA: 91.1
Isobutyryl-CoA: 76.4
Propionyl-CoA: 73.7
2-Methylbutyryl-CoA: 61.5
Benzoyl-CoA: 53.7
inactive: 52.6
Acetyl-CoA: 52.5
trans-1,2-CPDA: 50.3
fatty_acid: 47.1
CHC-CoA: 46.9
3-Methylbutyryl-CoA: 21.6

Link to PARAS substrate predictor