BGC0000153 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000153 (Stigmatella aurantiaca Sg a15)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS	1	66,808	High	stigmatellin	NRPS:Type I+PKS:Type I

BGC0000153 - Region 1 - T1PKS

Download region GenBank file

Download region SVG

Location: 1 - 66,808 nt. (total: 66,808 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 66,809
Label		BGC0000153

Tailoring functions

Oxidoreductases: oxidoreductase oxygenase

CAD19077.1: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

CAD19081.1: aldo/keto reductase family oxidoreductase

smcogs: SMCOG1039: aldo/keto reductase family oxidoreductase

stiL: monooxygenase FAD-binding

smcogs: SMCOG1050: monooxygenase FAD-binding

Transferases: acylase methyltransferase

CAD19079.1: methyltransferase

smcogs: SMCOG1089: methyltransferase

stiB: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

stiC: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

stiE: methyltransferase

smcogs: SMCOG1089: methyltransferase

stiG: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

stiH: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

stiK: methyltransferase

smcogs: SMCOG1089: methyltransferase

Ligases: synthase

stiA: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

stiD: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

stiF: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

stiJ: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000153.5	1.00	NRPS, PKS	stigmatellin	Stigmatella aurantiaca Sg a15
BGC0001276.3	0.77	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001273.3	0.67	PKS	asperlactone	Aspergillus ochraceus
BGC0001244.3	0.67	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001284.4	0.67	PKS	alternariol	Parastagonospora nodorum SN15
BGC0002191.2	0.67	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001275.3	0.67	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001164.3	0.65	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001254.3	0.65	PKS	ACT-Toxin II	Alternaria alternata
BGC0001160.5	0.65	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000153.5	1.00	NRPS, PKS	stigmatellin	Stigmatella aurantiaca Sg a15
BGC0001276.3	0.77	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001273.3	0.67	PKS	asperlactone	Aspergillus ochraceus
BGC0001244.3	0.67	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001284.4	0.67	PKS	alternariol	Parastagonospora nodorum SN15
BGC0002191.2	0.67	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001275.3	0.67	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001164.3	0.65	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001254.3	0.65	PKS	ACT-Toxin II	Alternaria alternata
BGC0001160.5	0.65	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002

Similar known clusters from MIBiG 4.0

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 66808:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (mal) + (ohmal) + (OMe-Me-ohmal) + (redmal) + (Me-mal) + (ohmal) + (ccmal) + (OMe-Me-ohmal)
Non-colinear ordering:: stiJ + stiB + stiE + stiF + stiG + stiH + stiC + stiD

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

stiA: Acetyl-CoA - mmal

PKS_AT (606..903): Acetyl-CoA

ATSignature: Acetyl-CoA

Top 3 matches:: Acetyl-CoA: 100.0%; CE-Malonyl-CoA: 58.3%; Methylmalonyl-CoA: 58.3%

minowa: Acetyl-CoA

Prediction, score:

Acetyl-CoA: 230.5
Methylmalonyl-CoA: 125.9
Methoxymalonyl-CoA: 104.0
Isobutyryl-CoA: 103.5
Ethylmalonyl-CoA: 100.6
Malonyl-CoA: 78.8
Propionyl-CoA: 77.7
Benzoyl-CoA: 75.4
trans-1,2-CPDA: 65.4
2-Methylbutyryl-CoA: 62.6
3-Methylbutyryl-CoA: 52.4
fatty_acid: 35.3
CHC-CoA: 34.1
inactive: 21.0

PKS_AT (1047..1343): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Malonyl-CoA: 79.2%; Methoxymalonyl-CoA: 79.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 188.9
Ethylmalonyl-CoA: 159.4
Methoxymalonyl-CoA: 139.7
Isobutyryl-CoA: 113.3
Benzoyl-CoA: 106.8
2-Methylbutyryl-CoA: 99.9
Malonyl-CoA: 97.6
trans-1,2-CPDA: 92.8
Propionyl-CoA: 86.2
CHC-CoA: 73.4
Acetyl-CoA: 69.3
3-Methylbutyryl-CoA: 52.4
fatty_acid: 42.1
inactive: 28.8

stiB: mal

PKS_AT (563..856): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 216.3
inactive: 162.9
Methylmalonyl-CoA: 83.0
Methoxymalonyl-CoA: 82.4
Propionyl-CoA: 63.2
Isobutyryl-CoA: 52.2
2-Methylbutyryl-CoA: 47.6
Ethylmalonyl-CoA: 45.5
Acetyl-CoA: 40.2
Benzoyl-CoA: 39.5
CHC-CoA: 29.8
trans-1,2-CPDA: 24.0
fatty_acid: 22.4
3-Methylbutyryl-CoA: 19.3

stiC: mal

PKS_AT (557..853): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 87.5%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 200.5
inactive: 146.7
Methoxymalonyl-CoA: 61.5
Methylmalonyl-CoA: 56.4
Isobutyryl-CoA: 39.4
Propionyl-CoA: 37.6
Acetyl-CoA: 32.2
Benzoyl-CoA: 27.2
CHC-CoA: 23.1
2-Methylbutyryl-CoA: 19.9
Ethylmalonyl-CoA: 18.8
trans-1,2-CPDA: 18.4
3-Methylbutyryl-CoA: 13.9
fatty_acid: 12.9

stiD: mmal

PKS_AT (581..878): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 83.3%; Malonyl-CoA: 79.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 180.8
Ethylmalonyl-CoA: 169.0
Methoxymalonyl-CoA: 139.5
Malonyl-CoA: 108.3
2-Methylbutyryl-CoA: 93.3
Propionyl-CoA: 88.6
Isobutyryl-CoA: 87.8
Benzoyl-CoA: 77.9
trans-1,2-CPDA: 76.8
CHC-CoA: 70.3
Acetyl-CoA: 63.3
fatty_acid: 52.4
3-Methylbutyryl-CoA: 31.4
inactive: 27.9

stiE: mmal

PKS_AT (566..862): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Ethylmalonyl-CoA: 83.3%; Malonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 190.2
Ethylmalonyl-CoA: 172.2
Methoxymalonyl-CoA: 152.8
Isobutyryl-CoA: 124.5
Benzoyl-CoA: 109.7
Malonyl-CoA: 104.3
trans-1,2-CPDA: 99.0
2-Methylbutyryl-CoA: 98.7
Acetyl-CoA: 80.1
CHC-CoA: 79.7
Propionyl-CoA: 75.3
3-Methylbutyryl-CoA: 48.3
inactive: 45.0
fatty_acid: 38.8

stiF: mal

PKS_AT (558..851): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 91.7%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 200.5
inactive: 156.1
Methylmalonyl-CoA: 59.5
Methoxymalonyl-CoA: 59.3
Isobutyryl-CoA: 40.7
Propionyl-CoA: 39.2
Benzoyl-CoA: 33.1
Acetyl-CoA: 20.4
Ethylmalonyl-CoA: 18.2
2-Methylbutyryl-CoA: 17.4
trans-1,2-CPDA: 16.8
CHC-CoA: 14.6
fatty_acid: 12.3
3-Methylbutyryl-CoA: 10.1

stiG: mmal

PKS_AT (578..875): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Ethylmalonyl-CoA: 83.3%; Malonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 185.2
Ethylmalonyl-CoA: 163.1
Methoxymalonyl-CoA: 145.5
Isobutyryl-CoA: 118.8
Malonyl-CoA: 106.3
trans-1,2-CPDA: 106.1
Benzoyl-CoA: 101.7
2-Methylbutyryl-CoA: 89.0
Propionyl-CoA: 74.8
Acetyl-CoA: 71.0
CHC-CoA: 68.5
3-Methylbutyryl-CoA: 53.2
fatty_acid: 33.5
inactive: 26.8

stiH: mal

PKS_AT (570..867): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 91.7%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 205.0
inactive: 155.2
Methoxymalonyl-CoA: 73.0
Methylmalonyl-CoA: 72.0
Propionyl-CoA: 58.5
2-Methylbutyryl-CoA: 49.8
Ethylmalonyl-CoA: 49.6
Isobutyryl-CoA: 46.1
Benzoyl-CoA: 42.1
CHC-CoA: 29.0
fatty_acid: 28.5
Acetyl-CoA: 26.5
trans-1,2-CPDA: 22.1
3-Methylbutyryl-CoA: 10.5

stiJ: mal

PKS_AT (565..861): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 91.7%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 200.0
inactive: 156.5
Methoxymalonyl-CoA: 74.4
Methylmalonyl-CoA: 70.0
Propionyl-CoA: 67.7
2-Methylbutyryl-CoA: 49.6
Ethylmalonyl-CoA: 41.5
Isobutyryl-CoA: 38.3
Benzoyl-CoA: 37.6
CHC-CoA: 32.4
trans-1,2-CPDA: 31.5
Acetyl-CoA: 21.5
fatty_acid: 19.2
3-Methylbutyryl-CoA: 11.1

Link to PARAS substrate predictor