BGC0000153: stigmatellin biosynthetic gene cluster from Stigmatella aurantiaca Sg a15
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Location: 1 - 66,808 nt. (total: 66,808 nt).
This entry is originally from NCBI GenBank AJ421825.1.

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core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
TTA codons
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Gene details
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General
Compounds
Genes
Polyketide
NRP
History
NRPS/PKS domains
KnownClusterBlast
General information about the BGC
MIBiG accession BGC0000153
Short description stigmatellin biosynthetic gene cluster from Stigmatella aurantiaca Sg a15
Status Minimal annotation: no
A minimal annotation only contains information on the BGC loci and one or more linked chemical product(s)

Completeness: complete
Whether the loci encodes everything needed for the pathway producing the compound(s)
Biosynthetic class(es)
  • NRP
  • Polyketide (Other)
Loci NCBI GenBank: AJ421825.1
Compounds
  • stigmatellin
Species Stigmatella aurantiaca Sg a15 [taxonomy]
References
Chemical products information
stigmatellin
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C30H42O7
Chemical database entries
NPAtlas
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • CAD19077.1
 4978 - 6231 (-) cytochrome P450 monooxygenase
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  • CAD19078.1
 6369 - 7730 (+) protein kinase
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  • CAD19079.1
 7849 - 8577 (-) methyl transferase
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  • CAD19080.1
 8622 - 8879 (-) acyl-CoA binding protein
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  • CAD19081.1
 8961 - 10070 (+) potassium channel beta chain
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  • CAD19082.1
 10428 - 10862 (+) ribosome binding factor
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  • CAD19083.1
 10859 - 12016 (+)
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  • CAD19084.1
 12082 - 13974 (-) cellulase
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  • CAD19085.1
  • stiA
 14345 - 21466 (+) StiA protein
  • Scaffold biosynthesis
Sequence-based prediction
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  • CAD19086.1
  • stiB
 21463 - 26220 (+) StiB protein
  • Scaffold biosynthesis
Sequence-based prediction
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  • CAD19087.1
  • stiC
 26284 - 31941 (+) StiC protein
  • Scaffold biosynthesis
Sequence-based prediction
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  • CAD19088.1
  • stiD
 31943 - 37738 (+) StiD protein
  • Scaffold biosynthesis
Sequence-based prediction
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  • CAD19089.1
  • stiE
 37740 - 43553 (+) StiE protein
  • Scaffold biosynthesis
Sequence-based prediction
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  • CAD19090.1
  • stiF
 43546 - 50202 (+) StiF protein
  • Scaffold biosynthesis
Sequence-based prediction
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  • CAD19091.1
  • stiG
 50205 - 54401 (+) StiG protein
  • Scaffold biosynthesis
Sequence-based prediction
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  • CAD19092.1
  • stiH
 54398 - 59212 (+) StiH protein
  • Scaffold biosynthesis
Sequence-based prediction
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  • CAD19093.1
  • stiJ
 59223 - 63002 (+) StiJ protein
  • Scaffold biosynthesis
Sequence-based prediction
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  • CAD19094.1
  • stiK
 63070 - 63840 (+) methyl transferase
  • Tailoring (Methylation)
Sequence-based prediction
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  • CAD19095.1
  • stiL
 63899 - 65470 (+) cytochrome P450 dependent monooxygenase
  • Tailoring (Oxidation)
Knock-out
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  • CAD19096.1
 65537 - 66808 (+) polynucleotide adenyltransferase
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Polyketide-specific information
Subclass Other
Starter unit Acetyl-CoA
Cyclic? no
Polyketide-synthases
Genes Properties Modules
stiB
+
stiD
+
stiG
+
stiE
+
stiF
+
stiH
+
stiA
+
stiC 		Synthase subclass: Modular type I
Module 1
Specificity: Methylmalonyl-CoA
Evidence for specificity: Structure-based inference
Genes: stiA
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Dehydratase, Enoylreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: L-OH
Module 2
Specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
Genes: stiB
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: L-OH
Module 3
Specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
Genes: stiC
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Dehydratase, Thiolation (ACP/PCP)
KR-domain stereochemistry: D-OH
Module 4
Specificity: Methylmalonyl-CoA
Evidence for specificity: Structure-based inference
Genes: stiD
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Thiolation (ACP/PCP)
Scaffold-modifying domains: Methylation
KR-domain stereochemistry: Unknown
Module 5
Specificity: Methylmalonyl-CoA
Evidence for specificity: Structure-based inference
Genes: stiD
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Thiolation (ACP/PCP)
Scaffold-modifying domains: Methylation
KR-domain stereochemistry: L-OH
Module 6
Specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
Genes: stiE
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Dehydratase, Enoylreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: L-OH
Module 7
Specificity: Methylmalonyl-CoA
Evidence for specificity: Structure-based inference
Genes: stiF
Core domains: Ketosynthase, Acyltransferase, Dehydratase, Thiolation (ACP/PCP)
Module 8
Specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
Genes: stiG
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: D-OH
Non-canonical activity:
  • Iterated
Evidence for non-canonical activity:
  • Structure-based inference
Module 9
Specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
Genes: stiH
Core domains: Ketosynthase, Acyltransferase, Thiolation (ACP/PCP)
Non-canonical activity:
  • Iterated
Evidence for non-canonical activity:
  • Structure-based inference
NRP-specific information
Subclass N/A
Cyclic? no
Annotation changelog
MIBiG version Submitter Notes
1.0
  • Hidden contributor (ID: 7SR74ARK6JXRBGJRGO2LUHE7, no GDPR consent given).
  • Submitted
2.0
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Migrated from v1.4
  • Updated compound(s) information (NPAtlas curation)
3.0
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Fixed incorrect gene identifiers
  • Removed ketoreductase stereochemistry annotation from modules without ketoreductases
  • Updated bioactivity data
3.1
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Update chemical activity to schema version 2.11
Detailed domain annotation
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