BGC0000153: stigmatellin biosynthetic gene cluster from Stigmatella aurantiaca Sg a15
Shows the layout of the region, marking coding sequences and areas of interest. Clicking a gene will select it and show any relevant details. Clicking an area feature (e.g. a candidate cluster) will select all coding sequences within that area. Double clicking an area feature will zoom to that area. Multiple genes and area features can be selected by clicking them while holding the Ctrl key.
Location: 1 - 66,808 nt. (total: 66,808 nt).
This entry is originally from NCBI GenBank AJ421825.1.

Legend:

core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
TTA codons
reset zoomreset view
zoomzoom to selection
Gene details
Shows details of the most recently selected gene, including names, products, location, and other annotations.
Select a gene to view the details available for it
General
Compounds
Genes
Polyketide
NRP
History
NRPS/PKS domains
KnownClusterBlast
General information about the BGC
MIBiG accession BGC0000153
Short description stigmatellin biosynthetic gene cluster from Stigmatella aurantiaca Sg a15
Status Minimal annotation: no
A minimal annotation only contains information on the BGC loci and one or more linked chemical product(s)

Completeness: complete
Whether the loci encodes everything needed for the pathway producing the compound(s)
Biosynthetic class(es)
  • NRP
  • Polyketide (Other)
Loci NCBI GenBank: AJ421825.1
Compounds
  • stigmatellin
Species Stigmatella aurantiaca Sg a15 [taxonomy]
References
Chemical products information
stigmatellin
Copy SMILES
C30H42O7
Chemical database entries
NPAtlas
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • CAD19077.1
4978 - 6231 (-) cytochrome P450 monooxygenase
copy AA seq
copy Nt seq
  • CAD19078.1
6369 - 7730 (+) protein kinase
copy AA seq
copy Nt seq
  • CAD19079.1
7849 - 8577 (-) methyl transferase
copy AA seq
copy Nt seq
  • CAD19080.1
8622 - 8879 (-) acyl-CoA binding protein
copy AA seq
copy Nt seq
  • CAD19081.1
8961 - 10070 (+) potassium channel beta chain
copy AA seq
copy Nt seq
  • CAD19082.1
10428 - 10862 (+) ribosome binding factor
copy AA seq
copy Nt seq
  • CAD19083.1
10859 - 12016 (+)
copy AA seq
copy Nt seq
  • CAD19084.1
12082 - 13974 (-) cellulase
copy AA seq
copy Nt seq
  • CAD19085.1
  • stiA
14345 - 21466 (+) StiA protein
  • Scaffold biosynthesis
Sequence-based prediction
copy AA seq
copy Nt seq
  • CAD19086.1
  • stiB
21463 - 26220 (+) StiB protein
  • Scaffold biosynthesis
Sequence-based prediction
copy AA seq
copy Nt seq
  • CAD19087.1
  • stiC
26284 - 31941 (+) StiC protein
  • Scaffold biosynthesis
Sequence-based prediction
copy AA seq
copy Nt seq
  • CAD19088.1
  • stiD
31943 - 37738 (+) StiD protein
  • Scaffold biosynthesis
Sequence-based prediction
copy AA seq
copy Nt seq
  • CAD19089.1
  • stiE
37740 - 43553 (+) StiE protein
  • Scaffold biosynthesis
Sequence-based prediction
copy AA seq
copy Nt seq
  • CAD19090.1
  • stiF
43546 - 50202 (+) StiF protein
  • Scaffold biosynthesis
Sequence-based prediction
copy AA seq
copy Nt seq
  • CAD19091.1
  • stiG
50205 - 54401 (+) StiG protein
  • Scaffold biosynthesis
Sequence-based prediction
copy AA seq
copy Nt seq
  • CAD19092.1
  • stiH
54398 - 59212 (+) StiH protein
  • Scaffold biosynthesis
Sequence-based prediction
copy AA seq
copy Nt seq
  • CAD19093.1
  • stiJ
59223 - 63002 (+) StiJ protein
  • Scaffold biosynthesis
Sequence-based prediction
copy AA seq
copy Nt seq
  • CAD19094.1
  • stiK
63070 - 63840 (+) methyl transferase
  • Tailoring (Methylation)
Sequence-based prediction
copy AA seq
copy Nt seq
  • CAD19095.1
  • stiL
63899 - 65470 (+) cytochrome P450 dependent monooxygenase
  • Tailoring (Oxidation)
Knock-out
copy AA seq
copy Nt seq
  • CAD19096.1
65537 - 66808 (+) polynucleotide adenyltransferase
copy AA seq
copy Nt seq
Polyketide-specific information
Subclass Other
Starter unit Acetyl-CoA
Cyclic? no
Polyketide-synthases
Genes Properties Modules
stiB
+
stiD
+
stiG
+
stiE
+
stiF
+
stiH
+
stiA
+
stiC
Synthase subclass: Modular type I
Module 1
Specificity: Methylmalonyl-CoA
Evidence for specificity: Structure-based inference
Genes: stiA
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Dehydratase, Enoylreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: L-OH
Module 2
Specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
Genes: stiB
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: L-OH
Module 3
Specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
Genes: stiC
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Dehydratase, Thiolation (ACP/PCP)
KR-domain stereochemistry: D-OH
Module 4
Specificity: Methylmalonyl-CoA
Evidence for specificity: Structure-based inference
Genes: stiD
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Thiolation (ACP/PCP)
Scaffold-modifying domains: Methylation
KR-domain stereochemistry: Unknown
Module 5
Specificity: Methylmalonyl-CoA
Evidence for specificity: Structure-based inference
Genes: stiD
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Thiolation (ACP/PCP)
Scaffold-modifying domains: Methylation
KR-domain stereochemistry: L-OH
Module 6
Specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
Genes: stiE
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Dehydratase, Enoylreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: L-OH
Module 7
Specificity: Methylmalonyl-CoA
Evidence for specificity: Structure-based inference
Genes: stiF
Core domains: Ketosynthase, Acyltransferase, Dehydratase, Thiolation (ACP/PCP)
Module 8
Specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
Genes: stiG
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: D-OH
Non-canonical activity:
  • Iterated
Evidence for non-canonical activity:
  • Structure-based inference
Module 9
Specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
Genes: stiH
Core domains: Ketosynthase, Acyltransferase, Thiolation (ACP/PCP)
Non-canonical activity:
  • Iterated
Evidence for non-canonical activity:
  • Structure-based inference
NRP-specific information
Subclass N/A
Cyclic? no
Annotation changelog
MIBiG version Submitter Notes
1.0
  • Hidden contributor (ID: 7SR74ARK6JXRBGJRGO2LUHE7, no GDPR consent given).
  • Submitted
2.0
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Migrated from v1.4
  • Updated compound(s) information (NPAtlas curation)
3.0
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Fixed incorrect gene identifiers
  • Removed ketoreductase stereochemistry annotation from modules without ketoreductases
  • Updated bioactivity data
3.1
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Update chemical activity to schema version 2.11
Detailed domain annotation
Shows NRPS- and PKS-related domains for each feature that contains them. Click on each domain for more information about the domain's location, consensus monomer prediction, and other details.
A glossary is available here.
Selected features only
Show module domains
Similar known gene clusters
Shows clusters from the MiBIG database that are similar to the current region. Genes marked with the same colour are interrelated. White genes have no relationship.
Click on reference genes to show details of similarities to genes within the current region.
Click on an accession to open that entry in the MiBIG database.