Genomic loci for this biosynthetic pathway

Cluster Type From To
The following clusters are from record BGC0000159.1:
Cluster 1Polyketide194050

BGC0000159, tautomycin biosynthetic gene cluster from Streptomyces spiroverticillatus. Locus 1. Full MIBiG entry.

Chemical compounds

Compound: tautomycin
PubChem ID: 3034761
SMILES string: Copy to clipboard
Molecular formula: C41H66O13
Average molecular mass: 766.966 Da
Molecular activity: Inhibitor
Molecular target: Protein Phosphatase

Class-specific details

Biosynthetic class(es):
Polyketide
Polyketide subclass:
Other (linear)
Polyketide synthase subclass:
Modular type I
Starter unit:
Isobutyryl-CoA
Thioesterase type:
Type I
Release / cyclization type:
Unknown

Modular polyketide synthases:
ttmJ (ABW96542)
Module 0
AT specificity: Isobutyryl-CoA
Evidence for specificity: None
Scaffold-modifying domain: None
Module 1
AT specificity: Methoxymalonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: Unknown
Scaffold-modifying domain: None
Module 2
AT specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: Unknown
Scaffold-modifying domain: None
Module 3
AT specificity: Methylmalonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: Unknown
Scaffold-modifying domain: None
Module 4
AT specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: Unknown
Scaffold-modifying domain: None
Module 5
AT specificity: Methylmalonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: Unknown
Scaffold-modifying domain: None
Module 6
AT specificity: Methylmalonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: Unknown
Scaffold-modifying domain: None

ttmI (ABW96541)
Module 7
AT specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: Unknown
Scaffold-modifying domain: None
Module 8
AT specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: Unknown
Scaffold-modifying domain: None
Module 9
AT specificity: Methylmalonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: Unknown
Scaffold-modifying domain: None

ttmH (ABW96540)
Module 10
AT specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: Unknown
Scaffold-modifying domain: None
Module 11
AT specificity: Methylmalonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: Unknown
Scaffold-modifying domain: None
Module 12
AT specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
Scaffold-modifying domain: None

Gene cluster description

tautomycin (BGC0000159). Gene Cluster 1. Biosynthetic class = Polyketide. GenBank EF990140, positions 7322-92509. Click on genes for more information.

Legend:

biosynthetic genes
transport-related genes
regulatory genes
other genes

Domain annotation

Homologous known gene clusters

General MIBiG information on this cluster

Complete gene cluster sequence?complete
Evidence for cluster-compound connection:Knock-out studies
MIxS-compliance:Unknown
Contact for this cluster:Xiaohui Yan (The Scripps Research Institute)

Literature references

1. Li W et al. (2006) Utilization of the methoxymalonyl-acyl carrier protein biosynthesis locus for cloning of the tautomycin biosynthetic gene cluster from Streptomyces spiroverticillatus. J Bacteriol 188(11):4148-52. doi: 10.1128/JB.00172-06.
2. Li W et al. (2008) Characterization of the tautomycin biosynthetic gene cluster from Streptomyces spiroverticillatus unveiling new insights into dialkylmaleic anhydride and polyketide biosynthesis. J Biol Chem 283(42):28607-17. doi: 10.1074/jbc.M804279200. Epub 2008
3. Ju J et al. (2009) Functional characterization of ttmM unveils new tautomycin analogs and insight into tautomycin biosynthesis and activity. Org Lett 11(7):1639-42. doi: 10.1021/ol900293j.