BGC0000164 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000164 (Streptomyces sp. NRRL 11266)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS	1	113,234	High	tetronomycin	PKS

BGC0000164 - Region 1 - T1PKS

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Location: 1 - 113,234 nt. (total: 113,234 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 113,235
Label		BGC0000164

Tailoring functions

Oxidoreductases: oxygenase

tmn14: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

tmn9: monooxygenase FAD-binding

smcogs: SMCOG1050: monooxygenase FAD-binding

tmnC: monooxygenase FAD-binding

smcogs: SMCOG1246: monooxygenase FAD-binding

Transferases: PPTase acylase methyltransferase

tmn12: methyltransferase

smcogs: SMCOG1089: methyltransferase

tmn23: 4'-phosphopantetheinyl transferase

smcogs: SMCOG1060: 4'-phosphopantetheinyl transferase

tmnVI: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

Hydrolases: esterase phosphatase

tmn16: FkbH like protein

smcogs: SMCOG1256: FkbH like protein

tmn6: thioesterase

smcogs: SMCOG1004: thioesterase

Lyases: cyclase lyase

tmn24: argininosuccinate lyase/adenylosuccinate lyase

smcogs: SMCOG1063: argininosuccinate lyase/adenylosuccinate lyase

tmn8: Diels-Alderase cyclase

extras: DA_cyclase: Diels-Alderase cyclase

Ligases: ligase synthase

tmn15: 3-oxoacyl-(acyl carrier protein) synthase III

smcogs: SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III

tmn20: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

tmnA1: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

tmnAII: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

tmnAIII: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

tmnAIV: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

tmnAV: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

tmnDI: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

tmnDII: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000164.5	1.00	PKS	tetronomycin	Streptomyces sp. NRRL 11266
BGC0001273.3	0.77	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	0.77	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001276.3	0.77	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001244.3	0.75	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001160.5	0.68	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0000067.5	0.68	PKS	geldanamycin	Streptomyces hygroscopicus subsp. duamyceticus
BGC0001164.3	0.68	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001254.3	0.67	PKS	ACT-Toxin II	Alternaria alternata
BGC0001284.4	0.67	PKS	alternariol	Parastagonospora nodorum SN15

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000164.5	1.00	PKS	tetronomycin	Streptomyces sp. NRRL 11266
BGC0001273.3	0.77	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	0.77	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001276.3	0.77	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001244.3	0.75	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001160.5	0.68	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0000067.5	0.68	PKS	geldanamycin	Streptomyces hygroscopicus subsp. duamyceticus
BGC0001164.3	0.68	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001254.3	0.67	PKS	ACT-Toxin II	Alternaria alternata
BGC0001284.4	0.67	PKS	alternariol	Parastagonospora nodorum SN15

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 113234:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (mal - mal - ohmal) + (ohmal) + (mal - ccmal - redmal) + (Me-ohmal) + (ccmal - ohmal) + (Me-redmal - Me-ccmal - ccmal) + (redmal - Me-redmal - Me-ccmal)
Non-colinear ordering:: tmnDI + tmnVI + tmnA1 + tmnAII + tmnAIII + tmnAIV + tmnAV

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

tmnA1: mal - mal - mal

PKS_AT (567..858): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 227.5
inactive: 107.8
Methoxymalonyl-CoA: 64.5
Methylmalonyl-CoA: 57.8
Propionyl-CoA: 32.2
Isobutyryl-CoA: 31.2
Ethylmalonyl-CoA: 28.3
Benzoyl-CoA: 17.5
Acetyl-CoA: 16.2
2-Methylbutyryl-CoA: 16.0
fatty_acid: 14.8
CHC-CoA: 13.2
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (1576..1861): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 193.7
inactive: 111.7
Methoxymalonyl-CoA: 78.8
Methylmalonyl-CoA: 70.7
Propionyl-CoA: 45.3
Isobutyryl-CoA: 36.5
fatty_acid: 31.8
CHC-CoA: 27.9
trans-1,2-CPDA: 22.1
Ethylmalonyl-CoA: 20.4
2-Methylbutyryl-CoA: 20.2
Acetyl-CoA: 20.1
Benzoyl-CoA: 18.5
3-Methylbutyryl-CoA: 0.0

PKS_AT (3334..3618): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 198.9
inactive: 116.1
Methoxymalonyl-CoA: 84.6
Methylmalonyl-CoA: 80.1
Propionyl-CoA: 57.3
Isobutyryl-CoA: 39.0
fatty_acid: 35.6
Ethylmalonyl-CoA: 33.8
CHC-CoA: 30.0
2-Methylbutyryl-CoA: 27.5
trans-1,2-CPDA: 21.3
Acetyl-CoA: 20.1
Benzoyl-CoA: 18.8
3-Methylbutyryl-CoA: 0.0

tmnVI: mal

PKS_AT (568..855): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 75.0%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 147.0
inactive: 60.5
Methoxymalonyl-CoA: 57.1
Methylmalonyl-CoA: 51.6
Propionyl-CoA: 25.7
fatty_acid: 21.6
Ethylmalonyl-CoA: 20.2
CHC-CoA: 12.5
Isobutyryl-CoA: 12.1
trans-1,2-CPDA: 11.3
Acetyl-CoA: 10.5
2-Methylbutyryl-CoA: 10.1
Benzoyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

tmnAII: mmal

PKS_AT (571..868): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Methoxymalonyl-CoA: 87.5%; Malonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 180.0
Ethylmalonyl-CoA: 151.3
Methoxymalonyl-CoA: 146.1
2-Methylbutyryl-CoA: 97.5
Malonyl-CoA: 87.7
trans-1,2-CPDA: 86.7
CHC-CoA: 85.9
Propionyl-CoA: 77.2
Isobutyryl-CoA: 58.8
Acetyl-CoA: 48.4
Benzoyl-CoA: 46.9
inactive: 30.5
3-Methylbutyryl-CoA: 26.2
fatty_acid: 24.7

tmnAIII: mal - mal

PKS_AT (566..875): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 79.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 167.3
inactive: 75.2
Methoxymalonyl-CoA: 55.3
Methylmalonyl-CoA: 42.7
Propionyl-CoA: 27.3
fatty_acid: 19.1
Ethylmalonyl-CoA: 18.7
trans-1,2-CPDA: 10.5
Isobutyryl-CoA: 10.4
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

PKS_AT (2336..2634): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 169.8
inactive: 90.5
Methoxymalonyl-CoA: 48.1
Methylmalonyl-CoA: 30.9
Propionyl-CoA: 25.9
fatty_acid: 25.6
Benzoyl-CoA: 16.9
Ethylmalonyl-CoA: 16.8
Acetyl-CoA: 16.5
2-Methylbutyryl-CoA: 14.3
Isobutyryl-CoA: 12.4
trans-1,2-CPDA: 10.6
CHC-CoA: 8.3
3-Methylbutyryl-CoA: 0.0

tmnAIV: mmal - mmal - mal

PKS_AT (577..868): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Malonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 176.5
Ethylmalonyl-CoA: 156.4
Methoxymalonyl-CoA: 145.4
trans-1,2-CPDA: 110.7
2-Methylbutyryl-CoA: 103.8
CHC-CoA: 100.9
Propionyl-CoA: 91.1
Malonyl-CoA: 84.8
Isobutyryl-CoA: 62.7
Benzoyl-CoA: 42.4
fatty_acid: 28.2
3-Methylbutyryl-CoA: 27.0
Acetyl-CoA: 20.9
inactive: 11.9

PKS_AT (2650..2941): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Malonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 178.9
Ethylmalonyl-CoA: 161.3
Methoxymalonyl-CoA: 146.3
2-Methylbutyryl-CoA: 106.2
trans-1,2-CPDA: 105.2
CHC-CoA: 101.2
Propionyl-CoA: 93.4
Malonyl-CoA: 87.3
Isobutyryl-CoA: 60.3
fatty_acid: 42.4
Benzoyl-CoA: 37.5
3-Methylbutyryl-CoA: 25.7
Acetyl-CoA: 24.7
inactive: 12.0

PKS_AT (4396..4681): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 75.0%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 149.4
Methoxymalonyl-CoA: 63.9
inactive: 62.1
Methylmalonyl-CoA: 50.4
Propionyl-CoA: 25.8
Ethylmalonyl-CoA: 23.2
fatty_acid: 20.0
Acetyl-CoA: 16.0
Isobutyryl-CoA: 15.8
trans-1,2-CPDA: 14.9
CHC-CoA: 12.0
2-Methylbutyryl-CoA: 7.5
Benzoyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

tmnAV: mal - mmal - mmal

PKS_AT (567..853): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 75.0%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 150.7
inactive: 66.4
Methoxymalonyl-CoA: 64.6
Methylmalonyl-CoA: 52.9
Ethylmalonyl-CoA: 25.6
Propionyl-CoA: 23.8
fatty_acid: 17.5
trans-1,2-CPDA: 13.5
CHC-CoA: 11.9
Acetyl-CoA: 11.3
Isobutyryl-CoA: 11.2
2-Methylbutyryl-CoA: 4.2
Benzoyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (2641..2935): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Malonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 177.5
Ethylmalonyl-CoA: 157.7
Methoxymalonyl-CoA: 145.0
2-Methylbutyryl-CoA: 107.5
trans-1,2-CPDA: 105.5
CHC-CoA: 101.3
Propionyl-CoA: 93.8
Malonyl-CoA: 87.0
Isobutyryl-CoA: 60.5
fatty_acid: 42.8
Benzoyl-CoA: 29.5
3-Methylbutyryl-CoA: 27.1
Acetyl-CoA: 26.1
inactive: 11.9

PKS_AT (4702..4992): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Malonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 177.5
Ethylmalonyl-CoA: 152.3
Methoxymalonyl-CoA: 147.5
trans-1,2-CPDA: 114.6
2-Methylbutyryl-CoA: 108.6
Propionyl-CoA: 95.6
CHC-CoA: 95.5
Malonyl-CoA: 87.7
Isobutyryl-CoA: 60.4
Benzoyl-CoA: 43.2
fatty_acid: 39.3
3-Methylbutyryl-CoA: 26.3
Acetyl-CoA: 17.5
inactive: 11.6

tmnDI: mal

PKS_AT (5..284): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 83.3%; inactive: 66.7%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 106.9
inactive: 38.5
Methoxymalonyl-CoA: 35.5
Methylmalonyl-CoA: 32.4
Propionyl-CoA: 28.9
fatty_acid: 22.2
2-Methylbutyryl-CoA: 17.2
Acetyl-CoA: 9.9
Isobutyryl-CoA: 5.5
trans-1,2-CPDA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

tmn20: NH2

CAL_domain (180..494): NH2

minowa: NH2

Prediction, score:

NH2: 46.0
fatty_acid: 25.6
shikimic_acid: 0.0
Acetyl-CoA: 0.0
AHBA: 0.0

Link to PARAS substrate predictor