BGC0000165 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000165 (Dactylosporangium aurantiacum subsp. hamdenensis)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS,PKS-like	1	101,746	High	tiacumicin B	PKS:Type I

BGC0000165 - Region 1 - PKS-like,T1PKS

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Location: 1 - 101,746 nt. (total: 101,746 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
PKS-like: (t2pks2 or ksIII)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

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TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 101,747
Label		BGC0000165

Tailoring functions

Oxidoreductases: dehydrogenase oxygenase

tiaJ: 3-hydroxybutyryl-CoA dehydrogenase

smcogs: SMCOG1095: 3-hydroxybutyryl-CoA dehydrogenase

tiaK: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

tiaP1: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

tiaP2: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

Transferases: acyltransferase glycosyltransferase halogenase transferase

tiaG1: glycosyltransferase, MGT family

smcogs: SMCOG1062: glycosyltransferase, MGT family

tiaG2: glycosyltransferase, MGT family

smcogs: SMCOG1062: glycosyltransferase, MGT family

tiaL: acetyl-CoA carboxylase, carboxyl transferase,

smcogs: SMCOG1059: acetyl-CoA carboxylase, carboxyl transferase,

tiaM: flavin-dependent cycline orsellinic halogenase

halogenases: flavin-dependent cycline orsellinic halogenase
smcogs: SMCOG1119: halogenase

tiaS6: acyltransferase 3

smcogs: SMCOG1088: acyltransferase 3

Hydrolases: esterase

tiaE: thioesterase

smcogs: SMCOG1004: thioesterase

Lyases: dehydratase

tiaS1: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

tiaS3: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

tiaS4: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

Ligases: synthase

tiaA1: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

tiaA2: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

tiaA3: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

tiaA4: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

tiaB: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

tiaD: 1-deoxy-D-xylulose-5-phosphate synthase

smcogs: SMCOG1110: 1-deoxy-D-xylulose-5-phosphate synthase

tiaF: 3-oxoacyl-(acyl carrier protein) synthase III

smcogs: SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000165.4	1.63	PKS	tiacumicin B	Dactylosporangium aurantiacum subsp. hamdenensis
BGC0001273.3	1.34	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	1.33	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	1.33	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001276.3	1.08	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001284.4	0.98	PKS	alternariol	Parastagonospora nodorum SN15
BGC0002154.2	0.98	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001257.3	0.98	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0001258.3	0.97	PKS	1,3,6,8-tetrahydroxynaphthalene	Glarea lozoyensis
BGC0002341.2	0.94	other (other)	3,5-dibromo-p-anisic acid	Planctomycetales bacterium 10988

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000165.4	1.00	PKS	tiacumicin B	Dactylosporangium aurantiacum subsp. hamdenensis
BGC0001276.3	0.77	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001275.3	0.77	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0002341.2	0.77	other (other)	3,5-dibromo-p-anisic acid	Planctomycetales bacterium 10988
BGC0001273.3	0.77	PKS	asperlactone	Aspergillus ochraceus
BGC0001244.3	0.76	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0002608.2	0.76	NRPS, PKS	ochratoxin A	Aspergillus steynii
BGC0000041.3	0.72	PKS	oronofacic acid	Pseudomonas syringae
BGC0002191.2	0.67	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001257.3	0.66	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 101746:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (pk - ohmal - Me-ccmal - Me-ccmal) + (pk - Me-ccmal) + (pk) + (ohmal) + (ccmal - Me-ccmal)
Non-colinear ordering:: tiaA1 + tiaA2 + tiaB + tiaA3 + tiaA4

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 0 - 40349:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 21490 - 101746:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (pk - ohmal - Me-ccmal - Me-ccmal) + (pk - Me-ccmal) + (ohmal) + (ccmal - Me-ccmal)
Non-colinear ordering:: tiaA1 + tiaA2 + tiaA3 + tiaA4

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

tiaB: pk

PKS_AT (542..827): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 79.2%; Methylmalonyl-CoA: 66.7%; Propionyl-CoA: 62.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 117.8
Ethylmalonyl-CoA: 94.2
Methoxymalonyl-CoA: 91.5
Isobutyryl-CoA: 72.8
Malonyl-CoA: 67.1
Propionyl-CoA: 53.6
fatty_acid: 48.2
trans-1,2-CPDA: 41.7
3-Methylbutyryl-CoA: 40.1
2-Methylbutyryl-CoA: 37.9
Benzoyl-CoA: 36.8
Acetyl-CoA: 35.0
CHC-CoA: 29.6
inactive: 17.4

tiaA1: pk - mal - mmal - mmal

PKS_AT (99..396): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 62.5%; Propionyl-CoA: 58.3%; Methylmalonyl-CoA: 58.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 129.5
Ethylmalonyl-CoA: 121.6
2-Methylbutyryl-CoA: 115.5
Methoxymalonyl-CoA: 112.5
Propionyl-CoA: 95.4
CHC-CoA: 84.3
Malonyl-CoA: 78.7
Isobutyryl-CoA: 68.1
trans-1,2-CPDA: 62.3
Acetyl-CoA: 54.4
Benzoyl-CoA: 38.2
inactive: 36.7
fatty_acid: 9.7
3-Methylbutyryl-CoA: 9.1

PKS_AT (1069..1332): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 66.7%; Propionyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 165.2
Methoxymalonyl-CoA: 66.2
inactive: 65.0
Methylmalonyl-CoA: 47.7
Isobutyryl-CoA: 40.9
fatty_acid: 33.4
Acetyl-CoA: 31.4
Propionyl-CoA: 28.3
Ethylmalonyl-CoA: 20.9
Benzoyl-CoA: 17.4
CHC-CoA: 16.9
3-Methylbutyryl-CoA: 11.8
2-Methylbutyryl-CoA: 11.3
trans-1,2-CPDA: 7.9

PKS_AT (2502..2779): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 91.7%; Methoxymalonyl-CoA: 87.5%; Ethylmalonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 169.2
Ethylmalonyl-CoA: 139.6
Methoxymalonyl-CoA: 129.6
2-Methylbutyryl-CoA: 74.1
trans-1,2-CPDA: 73.4
Propionyl-CoA: 72.3
Malonyl-CoA: 69.2
CHC-CoA: 56.2
Isobutyryl-CoA: 48.5
Benzoyl-CoA: 34.4
inactive: 24.6
Acetyl-CoA: 23.9
fatty_acid: 17.3
3-Methylbutyryl-CoA: 15.2

PKS_AT (4087..4364): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 91.7%; Methoxymalonyl-CoA: 87.5%; Ethylmalonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 169.0
Ethylmalonyl-CoA: 135.9
Methoxymalonyl-CoA: 131.1
2-Methylbutyryl-CoA: 84.2
Propionyl-CoA: 77.1
Malonyl-CoA: 74.7
trans-1,2-CPDA: 69.7
CHC-CoA: 59.0
Isobutyryl-CoA: 54.2
Benzoyl-CoA: 39.3
Acetyl-CoA: 34.6
fatty_acid: 28.8
inactive: 23.7
3-Methylbutyryl-CoA: 21.9

tiaA2: pk - mmal

PKS_AT (575..873): pk

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 87.5%; Ethylmalonyl-CoA: 83.3%; Methoxymalonyl-CoA: 83.3%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 174.5
Methylmalonyl-CoA: 174.4
Methoxymalonyl-CoA: 145.0
2-Methylbutyryl-CoA: 130.9
CHC-CoA: 106.8
Propionyl-CoA: 105.8
trans-1,2-CPDA: 101.8
Malonyl-CoA: 90.2
Isobutyryl-CoA: 81.0
Benzoyl-CoA: 55.9
fatty_acid: 46.8
Acetyl-CoA: 44.5
3-Methylbutyryl-CoA: 27.5
inactive: 11.2

PKS_AT (2072..2349): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 91.7%; Methoxymalonyl-CoA: 87.5%; Ethylmalonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 167.8
Ethylmalonyl-CoA: 141.6
Methoxymalonyl-CoA: 128.9
Propionyl-CoA: 74.7
2-Methylbutyryl-CoA: 73.6
trans-1,2-CPDA: 68.6
Malonyl-CoA: 67.0
CHC-CoA: 59.7
Isobutyryl-CoA: 45.4
Benzoyl-CoA: 31.1
Acetyl-CoA: 29.5
inactive: 28.2
fatty_acid: 24.0
3-Methylbutyryl-CoA: 12.1

tiaA3: mal

PKS_AT (545..809): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 66.7%; Propionyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 172.3
Methoxymalonyl-CoA: 72.8
inactive: 68.5
Methylmalonyl-CoA: 55.3
Isobutyryl-CoA: 48.0
fatty_acid: 44.6
Propionyl-CoA: 29.5
Acetyl-CoA: 26.6
Ethylmalonyl-CoA: 25.6
CHC-CoA: 22.3
Benzoyl-CoA: 19.4
2-Methylbutyryl-CoA: 17.1
trans-1,2-CPDA: 11.5
3-Methylbutyryl-CoA: 10.2

tiaA4: mal - mmal

PKS_AT (544..808): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 66.7%; Propionyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 170.2
Methoxymalonyl-CoA: 70.6
inactive: 68.8
Methylmalonyl-CoA: 53.6
Isobutyryl-CoA: 47.3
fatty_acid: 39.6
Propionyl-CoA: 30.2
Acetyl-CoA: 27.1
Ethylmalonyl-CoA: 22.7
CHC-CoA: 22.2
Benzoyl-CoA: 18.9
2-Methylbutyryl-CoA: 17.0
trans-1,2-CPDA: 11.1
3-Methylbutyryl-CoA: 10.5

PKS_AT (2130..2407): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 91.7%; Methoxymalonyl-CoA: 87.5%; Ethylmalonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 165.9
Ethylmalonyl-CoA: 130.4
Methoxymalonyl-CoA: 127.7
2-Methylbutyryl-CoA: 71.1
Propionyl-CoA: 68.5
trans-1,2-CPDA: 67.3
Malonyl-CoA: 66.8
CHC-CoA: 54.0
Isobutyryl-CoA: 47.9
Benzoyl-CoA: 33.4
fatty_acid: 26.9
Acetyl-CoA: 25.8
inactive: 22.4
3-Methylbutyryl-CoA: 16.6

Link to PARAS substrate predictor