BGC0000261 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000261 (Streptomyces sp. R1128)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T2PKS	1	17,200	High	R1128	PKS

BGC0000261 - Region 1 - T2PKS

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Location: 1 - 17,200 nt. (total: 17,200 nt) Show pHMM detection rules used

Region on contig edge.

T2PKS: (t2ks and t2clf)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 17,201
Label		BGC0000261

Tailoring functions

Oxidoreductases: oxygenase

zhuK: monooxygenase FAD-binding

smcogs: SMCOG1050: monooxygenase FAD-binding

zhuM: monooxygenase FAD-binding

smcogs: SMCOG1050: monooxygenase FAD-binding

Transferases: acylase carboxylase transferase

zhuC: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

zhuD: acetyl-CoA carboxylase biotin carboxylase

smcogs: SMCOG1090: acetyl-CoA carboxylase biotin carboxylase

zhuF: acetyl-CoA carboxylase, carboxyl transferase,

smcogs: SMCOG1059: acetyl-CoA carboxylase, carboxyl transferase,

Hydrolases: amidase

zhuL: amidase

smcogs: SMCOG1105: amidase

Lyases: cyclase

zhuI: cyclase/dehydrase

smcogs: SMCOG1128: cyclase/dehydrase

Ligases: synthase

zhuA: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

zhuB: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

zhuH: 3-oxoacyl-(acyl carrier protein) synthase III

smcogs: SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000261.5	1.00	PKS	R1128	Streptomyces sp. R1128
BGC0001284.4	0.65	PKS	alternariol	Parastagonospora nodorum SN15
BGC0000272.3	0.61	PKS	spore pigment	Streptomyces collinus
BGC0001197.5	0.55	PKS	frankiamicin	Frankia sp. EAN1pec
BGC0000271.5	0.54	PKS	spore pigment	Streptomyces avermitilis
BGC0000231.5	0.54	PKS	griseusin A, griseusin B	Streptomyces griseus
BGC0001513.3	0.52	PKS	anthrabenzoxocinone	Actinobacteria bacterium
BGC0000215.5	0.51	PKS	curamycin	Streptomyces cyaneus
BGC0001366.5	0.51	PKS	BE-24566B, zunyimycin A	Streptomyces sp. FJS31-2
BGC0000217.5	0.51	PKS	daunorubicin	Streptomyces sp.

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000261.5	1.00	PKS	R1128	Streptomyces sp. R1128
BGC0001284.4	0.65	PKS	alternariol	Parastagonospora nodorum SN15
BGC0000272.3	0.61	PKS	spore pigment	Streptomyces collinus
BGC0001197.5	0.55	PKS	frankiamicin	Frankia sp. EAN1pec
BGC0000271.5	0.54	PKS	spore pigment	Streptomyces avermitilis
BGC0000231.5	0.54	PKS	griseusin A, griseusin B	Streptomyces griseus
BGC0001513.3	0.52	PKS	anthrabenzoxocinone	Actinobacteria bacterium
BGC0000215.5	0.51	PKS	curamycin	Streptomyces cyaneus
BGC0001366.5	0.51	PKS	BE-24566B, zunyimycin A	Streptomyces sp. FJS31-2
BGC0000217.5	0.51	PKS	daunorubicin	Streptomyces sp.

Similar known clusters from MIBiG 4.0

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS substrates

Type II PKS

NRPS/PKS substrate predictions

zhuC: mal

PKS_AT (15..303): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 70.8%; inactive: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 68.7
Methoxymalonyl-CoA: 34.7
Methylmalonyl-CoA: 29.6
inactive: 19.5
Isobutyryl-CoA: 12.3
Propionyl-CoA: 12.1
2-Methylbutyryl-CoA: 11.1
CHC-CoA: 9.3
trans-1,2-CPDA: 9.1
fatty_acid: 0.0
Ethylmalonyl-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

Link to PARAS substrate predictor

Type II PKS gene cluster prediction details

Cluster section:: 0 to 17200
Starter unit(s):: acetyl-CoA (Score: 335.5; E-value: 0)
propionyl-CoA (Score: 335.5; E-value: 0)
isobutyryl-CoA (Score: 335.5; E-value: 0)
butyryl-CoA (Score: 335.5; E-value: 0)
Malonyl elongations:: 7 (Score: 747.1; E-value: 9.5e-228)
Product molecular weight(s):: acetyl-CoA_7: 321 Da
butyryl-CoA_7: 349 Da
isobutyryl-CoA_7: 349 Da
propionyl-CoA_7: 335 Da

zhuA: CLF 7 (Score: 747.1; E-value: 9.5e-228)
zhuB: KS (Score: 759.4; E-value: 2.1e-231)
zhuC: AT (Score: 415.9; E-value: 2.7e-127); AT AAG30190.1_R1128_acetyl-CoA/propionyl-CoA/isobutyryl-CoA/butyryl-CoA (Score: 674.0; E-value: 0.0)
zhuG: ACP (Score: 88.5; E-value: 5.1e-28)
zhuH: KSIII (Score: 413.3; E-value: 1.8e-126); KSIII AAG30195.1_R1128_acetyl-CoA/propionyl-CoA/isobutyryl-CoA/butyryl-CoA (Score: 668.0; E-value: 0.0)
zhuI: CYC C7-C12 (Score: 129.7; E-value: 2.4e-40)
zhuJ: CYC C5-C14/C3-C16 (Score: 340.2; E-value: 1.7e-104)
zhuK: OXY (Score: 508.4; E-value: 4.9e-155)
zhuM: OXY (Score: 488.4; E-value: 5.6e-149)
zhuN: ACP (Score: 123.2; E-value: 7.8e-39)