BGC0000319: cephamycin C biosynthetic gene cluster from Streptomyces clavuligerus ATCC 27064
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Location: 142,995 - 177,446 nt. (total: 34,452 nt).
This entry is originally from NCBI GenBank DS570624.1.

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core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
TTA codons
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Gene details
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General
Compounds
Genes
Biosynthesis
History
NRPS/PKS domains
KnownClusterBlast
General information about the BGC
MIBiG accession BGC0000319
Short description cephamycin C biosynthetic gene cluster from Streptomyces clavuligerus ATCC 27064
Status Quality: questionable
The quality level of this entry.

Status: active
The status of this entry.

Completeness: complete
Whether the entry covers everything needed for the pathway producing the compound(s)
Biosynthetic class(es)
  • NRPS (Type I)
Loci
DS570624.1
142995 - 177446
via Knock-out studies, Enzymatic assays, Heterologous expression
Compounds
  • cephamycin C
Species Streptomyces clavuligerus ATCC 27064 [taxonomy]
References
Chemical products information
cephamycin C Evidence:
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C16H22N4O9S1
Chemical database entries
NPAtlas
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • SSCG_00130
  • EDY47102.1
 142995 - 143993 (+) beta-lactamase
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    • SSCG_00131
    • EDY47103.1
    		 144030 - 146111 (-) D-alanyl-D-alanine carboxypeptidase
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      • SSCG_00132
      • EDY47104.1
      		 146465 - 147934 (-) cephamycin export protein cmcT
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        • SSCG_00133
        • EDY47105.1
        		 148322 - 149860 (-) aldehyde dehydrogenase
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          • SSCG_00134
          • EDY47106.1
          		 149876 - 150811 (-) deacetoxycephalosporin C synthetase
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            • SSCG_00135
            • EDY47107.1
            		 150890 - 152086 (-) isopenicillin N epimerase
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              • SSCG_00136
              • EDY47108.1
              		 152449 - 152568 (-) hypothetical protein
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                • SSCG_00137
                • EDY47109.1
                		 152861 - 153571 (+) conserved hypothetical protein
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                  • SSCG_00138
                  • EDY47110.1
                  		 153620 - 154552 (+) 7-alpha-cephem-methoxylase P8 chain
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                    • SSCG_00139
                    • EDY47111.1
                    		 154649 - 155605 (+) deacetoxycephalosporin C hydroxylase
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                      • SSCG_00140
                      • EDY47112.1
                      		 155681 - 157246 (+) 3'-hydroxymethylcephem-O-carbamoyltransferase
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                        • SSCG_00141
                        • EDY47113.1
                        		 158162 - 158950 (+) positive regulator
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                          • SSCG_00142
                          • EDY47114.1
                          		 159019 - 159195 (-) hypothetical protein
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                            • SSCG_00143
                            • EDY47115.1
                            		 159273 - 160289 (+) secreted protein
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                              • SSCG_00144
                              • EDY47116.1
                              		 160636 - 161184 (+) BLP
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                                • SSCG_00145
                                • EDY47117.1
                                		 161790 - 163163 (+) L-lysine-epsilon aminotransferase
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                                  • SSCG_00146
                                  • EDY47118.1
                                  		 163400 - 174661 (+) N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D- valine synthase
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                                    • SSCG_00147
                                    • EDY47119.1
                                    		 174693 - 175682 (+) isopenicillin N synthetase
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                                      • SSCG_00148
                                      • EDY47121.1
                                      		 175791 - 177446 (-) secreted penicillin binding protein
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                                        Biosynthesis information

                                        Biosynthetic modules

                                        Name
                                        0
                                        Type
                                        nrps-type1
                                        Genes
                                        SSCG_00146
                                        Substrates
                                        2-aminoadipic acid (evidence: Activity assay)
                                        Integrated Monomers
                                        Domains
                                        adenylation
                                        Name
                                        1
                                        Type
                                        nrps-type1
                                        Genes
                                        SSCG_00146
                                        Substrates
                                        cysteine (evidence: Activity assay)
                                        Integrated Monomers
                                        Domains
                                        condensation (DCL), adenylation
                                        Name
                                        2
                                        Type
                                        nrps-type1
                                        Genes
                                        SSCG_00146
                                        Substrates
                                        valine (evidence: Activity assay)
                                        Integrated Monomers
                                        Domains
                                        condensation (LCL), adenylation, epimerase
                                        Annotation changelog

                                        Entry version: 4

                                        Date
                                        Changes
                                        Submitters
                                        Reviewers
                                        2022-10-07
                                        Update chemical activity to schema version 2.11
                                        • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                        • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                                        Entry version: 3

                                        Date
                                        Changes
                                        Submitters
                                        Reviewers
                                        2022-09-15
                                        Corrected NRP module activity
                                        • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                        • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                        2022-09-15
                                        Updated NRP substrate specificities
                                        • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                        • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                                        Entry version: 2

                                        Date
                                        Changes
                                        Submitters
                                        Reviewers
                                        2019-10-16
                                        Migrated from v1.4
                                        • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                        • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                        2019-10-16
                                        Updated compound(s) information (NPAtlas curation)
                                        • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                        • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                                        Entry version: 1

                                        Date
                                        Changes
                                        Submitters
                                        Reviewers
                                        2015-06-12
                                        Submitted
                                        • (ID: SDHXYXULZ6ZTMHSITTYHRAHA)
                                        • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                        Detailed domain annotation
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