Genomic loci for this biosynthetic pathway

Cluster Type From To
The following clusters are from record BGC0000330.1:
Cluster 1NRP136362

BGC0000330, cupriachelin biosynthetic gene cluster from Ralstonia eutropha. Locus 1. Full MIBiG entry.

Chemical compounds

Compound: cupriachelin
SMILES string: Copy to clipboard
Molecular formula: C33H57N7O16
Exact molecular mass: 806.3788 Da ([M-H]-)
Molecular activity: Antibacterial, Siderophore

Class-specific details

Biosynthetic class(es):
NRP
NRP siderophore (linear)
Thioesterase type:
Type II
Release / cyclization type:
Hydrolysis

Nonribosomal peptide synthetases:
cucF (CAJ96472.1)
Module 0
A specificity: None
Evidence for specificity: Structure-based inference
C domain subtype: Starter
Scaffold-modifying domain: Oxidation
Module 1
A specificity: Aspartate
Evidence for specificity: Structure-based inference
C domain subtype: LCL
Scaffold-modifying domain: Oxidation
Module 2
A specificity: 2,4-Diaminobutyric acid
Evidence for specificity: Structure-based inference
C domain subtype: LCL
Scaffold-modifying domain: Oxidation
cucG (CAJ96471.1)
Module 3
A specificity: Aspartate
Evidence for specificity: Activity assay
C domain subtype: LCL
cucJ (CAJ96468.1)
Module 4
A specificity: Glycine
Evidence for specificity: Sequence-based prediction
C domain subtype: LCL
cucH (CAJ96470.1)
Module 5
A specificity: N5-Hydroxyornithine
Evidence for specificity: Sequence-based prediction
C domain subtype: LCL

Gene cluster description

cupriachelin (BGC0000330). Gene Cluster 1. Biosynthetic class = NRP. GenBank AM260480, positions 1886949-1923310. Click on genes for more information.

Legend:

biosynthetic genes
transport-related genes
regulatory genes
other genes

Domain annotation

Homologous known gene clusters

General MIBiG information on this cluster

Complete gene cluster sequence?complete
Evidence for cluster-compound connection:Knock-out studies, Enzymatic assays, Heterologous expression
MIxS-compliance:Unknown
Contact for this cluster:Markus Nett (Hans-Knoell-Institute)

Literature references

1. Kreutzer MF et al. (2012) Structure and biosynthetic assembly of cupriachelin, a photoreactive siderophore from the bioplastic producer Cupriavidus necator H16. J Am Chem Soc 134(11):5415-22. doi: 10.1021/ja300620z. Epub 2012 Mar