BGC0000355: fumiquinazoline A biosynthetic gene cluster from Aspergillus fumigatus Af293
Shows the layout of the region, marking coding sequences and areas of interest. Clicking a gene will select it and show any relevant details. Clicking an area feature (e.g. a candidate cluster) will select all coding sequences within that area. Double clicking an area feature will zoom to that area. Multiple genes and area features can be selected by clicking them while holding the Ctrl key.
View antiSMASH-generated output
Download Cluster GenBank file
Download region SVG
Location: 3,010,494 - 3,035,305 nt. (total: 24,812 nt).
This entry is originally from NCBI GenBank CM000174.1.

Legend:

core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
reset zoomreset view
zoomzoom to selection
Gene details
Shows details of the most recently selected gene, including names, products, location, and other annotations.
Select a gene to view the details available for it
General
Compounds
Genes
Biosynthesis
History
NRPS/PKS domains
KnownClusterBlast
General information about the BGC
MIBiG accession BGC0000355
Short description fumiquinazoline A biosynthetic gene cluster from Aspergillus fumigatus Af293
Status Quality: questionable
The quality level of this entry.

Status: active
The status of this entry.

Completeness: complete
Whether the entry covers everything needed for the pathway producing the compound(s)
Biosynthetic class(es)
  • NRPS (Type I)
Loci
CM000174.1
3010494 - 3035305
via Knock-out studies, Enzymatic assays, Heterologous expression
Compounds
  • fumiquinazoline A
  • fumiquinazoline C
  • fumiquinazoline D
Species Aspergillus fumigatus Af293 [taxonomy]
References
Chemical products information
fumiquinazoline A Evidence:
Copy SMILES
C24H23N5O4
Chemical database entries
NPAtlas
PubCHEM
fumiquinazoline C Evidence:
Copy SMILES
C24H21N5O4
Chemical database entries
NPAtlas
PubCHEM
fumiquinazoline D Evidence:
Copy SMILES
C24H21N5O4
Chemical database entries
PubCHEM
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • AFUA_6G12040
  • EAL89045.1
 3010494 - 3012341 (+) MFS transporter, putative
    copy AA seq
    copy Nt seq
    • AFUA_6G12050
    • EAL89046.1
    		 3013593 - 3017507 (+) nonribosomal peptide synthetase
    • Tailoring
    • Knock-out Activity assay
    copy AA seq
    copy Nt seq
    • AFUA_6G12060
    • EAL89047.1
    		 3017908 - 3019274 (-) FAD-dependent monooxygenase
    • Tailoring
    • Knock-out Activity assay
    copy AA seq
    copy Nt seq
    • AFUA_6G12070
    • EAL89048.1
    		 3020103 - 3021926 (+) FAD-dependent oxidoreductase
    • Tailoring
    • Knock-out Activity assay
    copy AA seq
    copy Nt seq
    • AFUA_6G12080
    • EAL89049.1
    		 3023316 - 3035305 (-) nonribosomal peptide synthetase
    • Scaffold biosynthesis
    • Knock-out Activity assay
    copy AA seq
    copy Nt seq
    Biosynthesis information

    Biosynthetic modules

    Name
    1
    Type
    nrps-type1
    Genes
    AFUA_6G12080 AFUA_6G12050
    Substrates
    anthanillic acid, benzoic acid, 2-chlorobenzoic acid, 4-chlorobenzoic acid, 3-hydroxybenzoic acid, 3-aminobenzoic acid (evidence: Structure-based inference, ATP-PPi exchange assay)
    Integrated Monomers
    Domains
    condensation (LCL), adenylation
    Name
    2
    Type
    nrps-type1
    Genes
    AFUA_6G12080
    Substrates
    tryptophan (evidence: Structure-based inference [1])
    Integrated Monomers
    Domains
    condensation (LCL), adenylation, epimerase
    Name
    3
    Type
    nrps-type1
    Genes
    AFUA_6G12080
    Substrates
    alanine (evidence: Structure-based inference [1])
    Integrated Monomers
    Domains
    condensation (Heterocyclization), adenylation
    Annotation changelog

    Entry version: 4

    Date
    Changes
    Submitters
    Reviewers
    2022-10-07
    Update chemical activity to schema version 2.11
    • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
    • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

    Entry version: 3

    Date
    Changes
    Submitters
    Reviewers
    2022-09-15
    Cleaned up synonym list
    • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
    • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
    2022-09-15
    Corrected NRP module activity
    • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
    • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
    2022-09-15
    Corrected gene names referencing other accessions
    • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
    • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
    2022-09-15
    Removed thioesterase annotation for unknown gene
    • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
    • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
    2022-09-15
    Removed inconsistent additional gene names
    • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
    • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
    2022-09-15
    Updated NRP substrate specificities
    • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
    • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

    Entry version: 2

    Date
    Changes
    Submitters
    Reviewers
    2019-10-16
    Migrated from v1.4
    • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
    • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
    2019-10-16
    Updated compound(s) information (NPAtlas curation)
    • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
    • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

    Entry version: 1

    Date
    Changes
    Submitters
    Reviewers
    2015-06-12
    Submitted
    • (ID: J4R6BDENZAM7XVDGVEEHZB2Y)
    • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
    Detailed domain annotation
    Shows NRPS- and PKS-related domains for each feature that contains them. Click on each domain for more information about the domain's location, consensus monomer prediction, and other details.
    A domain glossary is available here, and an explanation of the visualisation is available here.
    Selected features only
    Show module domains
    Similar known gene clusters from MIBiG 4.0
    Shows clusters from the MiBIG database that are similar to the current region. Genes marked with the same colour are interrelated. White genes have no relationship.
    Click on reference genes to show details of similarities to genes within the current region.
    Click on an accession to open that entry in the MiBIG database.