BGC0000399: orfamide A biosynthetic gene cluster from Pseudomonas protegens Pf-5
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Location: 2,364,934 - 2,411,953 nt. (total: 47,020 nt).
This entry is originally from NCBI GenBank CP000076.1.

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core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
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General
Compounds
Genes
Biosynthesis
History
NRPS/PKS domains
KnownClusterBlast
General information about the BGC
MIBiG accession BGC0000399
Short description orfamide A biosynthetic gene cluster from Pseudomonas protegens Pf-5
Status Quality: questionable
The quality level of this entry.

Status: active
The status of this entry.

Completeness: complete
Whether the entry covers everything needed for the pathway producing the compound(s)
Biosynthetic class(es)
  • NRPS (Type I)
Loci
CP000076.1
2364934 - 2411953
via Knock-out studies, Gene expression correlated with compound production
Compounds
  • orfamide A
  • orfamide C
Species Pseudomonas protegens Pf-5 [taxonomy]
References
Chemical products information
orfamide A Evidence:
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C64H114N10O17
Chemical database entries
NPAtlas
orfamide C Evidence:
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C62H110N10O17
Chemical database entries
NPAtlas
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • PFL_2142
  • AAY91416.1
 2364934 - 2366364 (-) efflux transporter, outer membrane factor lipoprotein, NodT family
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    • PFL_2143
    • AAY91417.1
    		 2366424 - 2367224 (-) transcriptional regulator, LuxR family
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      • PFL_2144
      • AAY91418.1
      		 2367557 - 2367703 (-) conserved hypothetical protein
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        • PFL_2145
        • AAY91419.3
        • ofaA
        		 2367819 - 2374184 (+) non-ribosomal peptide synthetase OfaA
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          • PFL_2146
          • AAY91420.2
          • ofaB
          		 2374502 - 2387605 (+) non-ribosomal peptide synthetase OfaB
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            • PFL_2147
            • AAY91421.3
            • ofaC
            		 2387602 - 2402307 (+) non-ribosomal peptide synthetase OfaC
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              • PFL_2148
              • AAY91422.1
              		 2402458 - 2403609 (+) efflux transporter, RND family, MFP subunit
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                • PFL_2149
                • AAY91423.1
                		 2403612 - 2405570 (+) efflux ABC transporter, permease/ATP-binding protein
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                  • PFL_2150
                  • AAY91424.1
                  		 2405631 - 2406419 (-) transcriptional regulator, LuxR family
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                    • PFL_2151
                    • AAY91425.1
                    		 2406647 - 2407060 (-) glyoxalase family protein
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                      • PFL_2152
                      • AAY91426.1
                      		 2407236 - 2408744 (-) radical SAM domain protein
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                        • PFL_2153
                        • AAY91427.1
                        		 2408835 - 2409443 (-) conserved hypothetical protein
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                          • PFL_2154
                          • AAY91428.1
                          		 2409495 - 2410130 (-) conserved hypothetical protein
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                            • PFL_2155
                            • AAY91429.1
                            		 2410167 - 2410355 (-) conserved hypothetical protein
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                              • PFL_2156
                              • AAY91430.1
                              		 2410673 - 2411302 (-) HAD hydrolase, family IA, variant 3
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                                • PFL_2157
                                • AAY91431.1
                                		 2411415 - 2411672 (+) conserved hypothetical protein
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                                  • PFL_2158
                                  • AAY91432.1
                                  		 2411768 - 2411953 (+) conserved hypothetical protein
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                                    Biosynthesis information

                                    Biosynthetic modules

                                    Name
                                    1
                                    Type
                                    nrps-type1
                                    Genes
                                    ofaA
                                    Substrates
                                    leucine (evidence: Structure-based inference, Sequence-based prediction)
                                    Integrated Monomers
                                    Domains
                                    condensation (Starter), adenylation
                                    Name
                                    2
                                    Type
                                    nrps-type1
                                    Genes
                                    ofaA
                                    Substrates
                                    glutamic acid (evidence: Structure-based inference, Sequence-based prediction)
                                    Integrated Monomers
                                    Domains
                                    condensation (Dual), adenylation, epimerase
                                    Name
                                    3
                                    Type
                                    nrps-type1
                                    Genes
                                    ofaB
                                    Substrates
                                    threonine (evidence: Structure-based inference, Sequence-based prediction)
                                    Integrated Monomers
                                    Domains
                                    condensation (Dual), adenylation, epimerase
                                    Name
                                    4
                                    Type
                                    nrps-type1
                                    Genes
                                    ofaB
                                    Substrates
                                    isoleucine (evidence: Structure-based inference, Sequence-based prediction)
                                    Integrated Monomers
                                    Domains
                                    condensation (Dual), adenylation, epimerase
                                    Name
                                    5
                                    Type
                                    nrps-type1
                                    Genes
                                    ofaB
                                    Substrates
                                    leucine (evidence: Structure-based inference, Sequence-based prediction)
                                    Integrated Monomers
                                    Domains
                                    condensation (Dual), adenylation
                                    Name
                                    6
                                    Type
                                    nrps-type1
                                    Genes
                                    ofaB
                                    Substrates
                                    serine (evidence: Structure-based inference, Sequence-based prediction)
                                    Integrated Monomers
                                    Domains
                                    condensation (Dual), adenylation, epimerase
                                    Name
                                    7
                                    Type
                                    nrps-type1
                                    Genes
                                    ofaC
                                    Substrates
                                    leucine (evidence: Structure-based inference, Sequence-based prediction)
                                    Integrated Monomers
                                    Domains
                                    condensation (Dual), adenylation
                                    Name
                                    8
                                    Type
                                    nrps-type1
                                    Genes
                                    ofaC
                                    Substrates
                                    leucine (evidence: Structure-based inference, Sequence-based prediction)
                                    Integrated Monomers
                                    Domains
                                    condensation (LCL), adenylation
                                    Name
                                    9
                                    Type
                                    nrps-type1
                                    Genes
                                    ofaC
                                    Substrates
                                    serine (evidence: Structure-based inference, Sequence-based prediction)
                                    Integrated Monomers
                                    Domains
                                    condensation (LCL), adenylation, epimerase
                                    Name
                                    10
                                    Type
                                    nrps-type1
                                    Genes
                                    ofaC
                                    Substrates
                                    valine (evidence: Structure-based inference, Sequence-based prediction)
                                    Integrated Monomers
                                    Domains
                                    condensation (Dual), adenylation
                                    Annotation changelog

                                    Entry version: 4

                                    Date
                                    Changes
                                    Submitters
                                    Reviewers
                                    2022-10-07
                                    Update chemical activity to schema version 2.11
                                    • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                    • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                                    Entry version: 3

                                    Date
                                    Changes
                                    Submitters
                                    Reviewers
                                    2022-09-15
                                    Corrected NRP module activity
                                    • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                    • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                    2022-09-15
                                    Corrected gene identifiers
                                    • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                    • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                    2022-09-15
                                    Updated NRP substrate specificities
                                    • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                    • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                                    Entry version: 2

                                    Date
                                    Changes
                                    Submitters
                                    Reviewers
                                    2019-10-16
                                    Migrated from v1.4
                                    • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                    • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                    2019-10-16
                                    Updated compound(s) information (NPAtlas curation)
                                    • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                    • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                                    Entry version: 1

                                    Date
                                    Changes
                                    Submitters
                                    Reviewers
                                    2015-06-12
                                    Submitted
                                    • (ID: KRKW7VUNYGF36O3Z7FAQ6YXG)
                                    • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                    Detailed domain annotation
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