BGC0000399 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000399 (Pseudomonas protegens Pf-5)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	NRPS	1	47,020	orfamide A/orfamide C	NRPS:Type I	100%

BGC0000399 - Region 1 - NRPS

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Location: 1 - 47,020 nt. (total: 47,020 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))

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core biosynthetic genes

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transport-related genes

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Gene details

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Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 47,021
Label		BGC0000399

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000399	1.00	NRP	orfamide A, orfamide C	Pseudomonas protegens Pf-5
BGC0002522	0.54	NRP	viscosinamide A, pseudodesmin A	Pseudomonas sp.
BGC0000398	0.53	NRP	orfamide B	Pseudomonas sp. CMR12a
BGC0001346	0.52	NRP	bananamide 1, bananamide 2, bananamide 3	Pseudomonas fluorescens
BGC0002548	0.52	NRP	bananamide F, bananamide D, bananamide G, bananamide E	Pseudomonas sp.
BGC0001842	0.51	NRP	gacamide A	Pseudomonas fluorescens Pf0-1
BGC0002092	0.50	NRP	stechlisin B2, stechlisin C3, stechlisin D3, tensin, stechlisin E2, stechlisin F	Pseudomonas sp.
BGC0000411	0.48	NRP	putisolvin	Pseudomonas putida
BGC0000435	0.45	NRP	syringafactin	Pseudomonas syringae pv. tomato str. DC3000
BGC0000323	0.45	NRP	cichofactin A, cichofactin B	Pseudomonas cichorii

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000399	1.00	NRP	orfamide A, orfamide C	Pseudomonas protegens Pf-5
BGC0002522	0.81	NRP	viscosinamide A, pseudodesmin A	Pseudomonas sp.
BGC0000398	0.80	NRP	orfamide B	Pseudomonas sp. CMR12a
BGC0001346	0.80	NRP	bananamide 1, bananamide 2, bananamide 3	Pseudomonas fluorescens
BGC0002548	0.80	NRP	bananamide F, bananamide D, bananamide G, bananamide E	Pseudomonas sp.
BGC0001842	0.79	NRP	gacamide A	Pseudomonas fluorescens Pf0-1
BGC0002092	0.79	NRP	stechlisin B2, stechlisin C3, stechlisin D3, tensin, stechlisin E2, stechlisin F	Pseudomonas sp.
BGC0000411	0.77	NRP	putisolvin	Pseudomonas putida
BGC0000435	0.75	NRP	syringafactin	Pseudomonas syringae pv. tomato str. DC3000
BGC0000323	0.75	NRP	cichofactin A, cichofactin B	Pseudomonas cichorii

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 47020:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Leu - Glu) + (Thr - Ile - Leu - Ser) + (Leu - Leu - Ser - Val)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

PFL_2145

Search NORINE for peptide: strict or relaxed

AMP-binding (473..861): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: leu (Leu)

Stachelhaus prediction details:

Stachelhaus sequence:: DAWFLGNVVK
Nearest Stachelhaus code(s):: Leu DAWFLGNVVK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1528..1928): Glu

nrpys: Glu

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asp (Asp)

Stachelhaus prediction details:

Stachelhaus sequence:: DGWKFGVVDK
Nearest Stachelhaus code(s):: Glu DGWKFGVVDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

PFL_2146

Search NORINE for peptide: strict or relaxed

AMP-binding (554..958): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: DFWNIGMVHK
Nearest Stachelhaus code(s):: Thr DFWNIGMVHK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1639..2034): Ile

nrpys: Ile

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: ile (Ile)

Stachelhaus prediction details:

Stachelhaus sequence:: DAMFLGCTYK
Nearest Stachelhaus code(s):: Ile DAMFLGCTYK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2716..3106): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: leu (Leu)

Stachelhaus prediction details:

Stachelhaus sequence:: DAWFLGNVVK
Nearest Stachelhaus code(s):: Leu DAWFLGNVVK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (3783..4185): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: DVWHMSLVDK
Nearest Stachelhaus code(s):: Ser DVWHMSLVDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

PFL_2147

Search NORINE for peptide: strict or relaxed

AMP-binding (568..957): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: leu (Leu)

Stachelhaus prediction details:

Stachelhaus sequence:: DAWFLGNVVK
Nearest Stachelhaus code(s):: Leu DAWFLGNVVK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1611..2001): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: leu (Leu)

Stachelhaus prediction details:

Stachelhaus sequence:: DAWFLGNVVK
Nearest Stachelhaus code(s):: Leu DAWFLGNVVK (100% 8Å match); Leu or Ile DAWFLGNVVK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2658..3060): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: DVWHMSLVDK
Nearest Stachelhaus code(s):: Ser DVWHMSLVDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (3743..4143): Val

nrpys: Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: ile (Ile)

Stachelhaus prediction details:

Stachelhaus sequence:: DALFLGGTFK
Nearest Stachelhaus code(s):: Val DALFLGGTFK (100% 8Å match)
Stachelhaus code match:: 100% (strong)