Genomic loci for this biosynthetic pathway

Cluster Type From To
The following clusters are from record BGC0000419.1:
Cluster 1NRP / Saccharide168710

BGC0000419, ristomycin A biosynthetic gene cluster from Amycolatopsis japonica. Locus 1. Full MIBiG entry.

Chemical compounds

Compound: Ristomycin A
PubChem ID: 16131418
SMILES string: Copy to clipboard
Molecular formula: C95H110N8O44
Average molecular mass: 2067.937 Da
Molecular activity: Antibacterial
Molecular target: Cell wall, specifically the D-alanyl-D-alanine terminus of peptidoglycan

Class-specific details

Biosynthetic class(es):
NRP / Saccharide
Glycopeptide (linear)
Thioesterase type:
Unknown
Release / cyclization type:
None

Nonribosomal peptide synthetases:
AIG79243.1
Module 1
A specificity: Hydroxyphenylglycine
Evidence for specificity: Structure-based inference
C domain subtype: Other
Module 2
A specificity: Beta-hydroxytyrosine
Evidence for specificity: Structure-based inference
AA is epimerized
C domain subtype: N/A
AIG79242.1
Module 2
A specificity: Unknown
Evidence for specificity: Unknown
C domain subtype: Other
Module 3
A specificity: 3,5-Dihydroxyphenylglycine
Evidence for specificity: Structure-based inference
C domain subtype: N/A
AIG79241.1
Module 3
A specificity: Unknown
Evidence for specificity: Unknown
C domain subtype: Other
Module 4
A specificity: 4-Hydroxyphenylglycine
Evidence for specificity: Structure-based inference
AA is epimerized
C domain subtype: Other
Module 5
A specificity: 4-Hydroxyphenylglycine
Evidence for specificity: Structure-based inference
AA is epimerized
C domain subtype: Other
Module 6
A specificity: Beta-hydroxytyrosine
Evidence for specificity: Structure-based inference
C domain subtype: N/A
AIG79240.1
Module 6
A specificity: Unknown
Evidence for specificity: Unknown
C domain subtype: Other
Module 7
A specificity: 3,5-Dihydroxyphenylglycine
Evidence for specificity: Structure-based inference
C domain subtype: Other
Saccharide subclass:
other
Glycosyltransferases:
ABJ90158.1:
Specificity: D-galacturonic acid
Evidence for specificity: Activity assay
Sugar biosynthesis subcluster: MOEE5

Gene cluster description

ristomycin A (BGC0000419). Gene Cluster 1. Biosynthetic class = NRP/Saccharide. GenBank CP008953, positions 6816619-6885328. Click on genes for more information.

Legend:

biosynthetic genes
transport-related genes
regulatory genes
other genes

Domain annotation

Homologous known gene clusters

General MIBiG information on this cluster

Complete gene cluster sequence?complete
Evidence for cluster-compound connection:Gene expression correlated with compound production, Sequence-based prediction
MIxS-compliance:Unknown
Contact for this cluster:Dr. Evi Stegmann (University of Tuebingen, Department of Microbiology/Biotechnology)

Literature references

1. Spohn M et al. (2014) Overproduction of Ristomycin A by activation of a silent gene cluster in Amycolatopsis japonicum MG417-CF17. Antimicrob Agents Chemother 58(10):6185-96. doi: 10.1128/AAC.03512-14.