BGC0000440 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000440 (Actinoplanes teichomyceticus)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	NRPS,T3PKS	1	89,713	High	Teicoplanin A2-1/Teicoplanin A2-2/Teicoplanin A2-3/Teicoplanin A2-4/Teicoplanin A2-5	NRPS:Type I

BGC0000440 - Region 1 - NRPS,T3PKS

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Location: 1 - 89,713 nt. (total: 89,713 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 89,714
Label		BGC0000440

Tailoring functions

Oxidoreductases: dehydrogenase oxygenase

tcp18: Cyclization

MITE: MITE0000038Cyclization (identity: 74.90%)
smcogs: SMCOG1007: cytochrome P450

tcp19: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

tcp2: dehydrogenase

smcogs: SMCOG1072: dehydrogenase

tcp20: Cyclization

MITE: MITE0000041Cyclization (identity: 73.10%)
smcogs: SMCOG1007: cytochrome P450

tcp22: Cyclization

MITE: MITE0000027Cyclization (identity: 70.80%)
smcogs: SMCOG1007: cytochrome P450

tei9: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Transferases: acyltransferase aminotransferase glycosyltransferase halogenase

tcp11: polyketide synthase associated protein papA3

smcogs: SMCOG1209: polyketide synthase associated protein papA3

tcp21: flavin-dependent tyrosine-like hpg halogenase

halogenases: flavin-dependent tyrosine-like hpg halogenase
MITE: MITE0000028Halogenation (identity: 84.50%)
smcogs: SMCOG1119: halogenase

tcp23: Glycosylation

MITE: MITE0000033Glycosylation (identity: 100.00%)
smcogs: SMCOG1102: glycosyltransferase

tcp36: aminotransferase

smcogs: SMCOG1019: aminotransferase

tcp8: Glycosylation

MITE: MITE0000034Glycosylation (identity: 100.00%)
smcogs: SMCOG1102: glycosyltransferase

Hydrolases: Acylation esterase

tcp24: Acylation, Hydrolysis

MITE: MITE0000035Acylation, Hydrolysis (identity: 100.00%)

tcp39: thioesterase

smcogs: SMCOG1004: thioesterase

Lyases: hydratase

tcp32: enoyl-CoA hydratase

smcogs: SMCOG1023: enoyl-CoA hydratase

tcp33: enoyl-CoA hydratase

smcogs: SMCOG1023: enoyl-CoA hydratase

Ligases: Acylation ligase synthase

tcp10: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

tcp12: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

tcp24: Acylation, Hydrolysis

MITE: MITE0000035Acylation, Hydrolysis (identity: 100.00%)

tcp26: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

tcp30: chalcone and stilbene synthase domain protein

smcogs: SMCOG1024: chalcone and stilbene synthase domain protein

tcp9: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000440.5	1.18	NRPS	Teicoplanin A2-1, Teicoplanin A2-2, Teicoplanin A2-3, Teicoplanin A2-4, Teicoplanin A2-5	Actinoplanes teichomyceticus
BGC0000441.4	0.97	NRPS	teicoplanin	Actinoplanes teichomyceticus
BGC0002341.2	0.94	other (other)	3,5-dibromo-p-anisic acid	Planctomycetales bacterium 10988
BGC0002608.2	0.91	NRPS, PKS	ochratoxin A	Aspergillus steynii
BGC0001164.3	0.53	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001160.5	0.53	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0002347.3	0.47	other (other)	cyanogramide, cyanogramide B, cyanogramide C, cyanogramide D	Actinoalloteichus caeruleus WH1-2216-6
BGC0002084.4	0.46	ribosomal	biarylitide YYH	Planomonospora sp. ID82291
BGC0002099.3	0.46	ribosomal	biarylitide YFH	Planomonospora sp. ID107089
BGC0000322.3	0.45	NRPS	chloroeremomycin	Amycolatopsis orientalis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000440.5	0.99	NRPS	Teicoplanin A2-1, Teicoplanin A2-2, Teicoplanin A2-3, Teicoplanin A2-4, Teicoplanin A2-5	Actinoplanes teichomyceticus
BGC0000441.4	0.92	NRPS	teicoplanin	Actinoplanes teichomyceticus
BGC0002341.2	0.77	other (other)	3,5-dibromo-p-anisic acid	Planctomycetales bacterium 10988
BGC0002608.2	0.76	NRPS, PKS	ochratoxin A	Aspergillus steynii
BGC0001261.3	0.75	NRPS	AM-toxin	Alternaria alternata
BGC0001249.5	0.75	NRPS	dimethylcoprogen	Alternaria alternata
BGC0001132.4	0.74	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002135.2	0.73	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001128.4	0.73	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002286.3	0.73	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 621 - 89713:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Hpg - D-Tyr) + (Dhpg) + (D-Hpg - D-Hpg - Tyr) + (Dhpg)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 621 - 67898:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Hpg - D-Tyr) + (Dhpg) + (D-Hpg - D-Hpg - Tyr) + (Dhpg)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

tcp9: Hpg - Tyr

Search NORINE for peptide: strict or relaxed

AMP-binding (15..402): Hpg

nrpys: Hpg

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: Polar, uncharged (hydroxy-phenyl) (Dhpg, Hpg)
Single AA prediction:: hpg (Hpg)

Stachelhaus prediction details:

Stachelhaus sequence:: DAFHLGLLCK
Nearest Stachelhaus code(s):: Hpg DAFHLGLLCK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1037..1423): Tyr

nrpys: Tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Polar aromatic ring (Tyr, bOH-Tyr)
Single AA prediction:: tyr (Tyr)

Stachelhaus prediction details:

Stachelhaus sequence:: DASTVAAVCK
Nearest Stachelhaus code(s):: Tyr DASTVAAVCK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

tcp10: Dhpg

Search NORINE for peptide: strict or relaxed

AMP-binding (466..854): Dhpg

nrpys: Dhpg

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: Polar, uncharged (hydroxy-phenyl) (Dhpg, Hpg)
Single AA prediction:: dhpg (Dhpg)

Stachelhaus prediction details:

Stachelhaus sequence:: DAYNLGTLCK
Nearest Stachelhaus code(s):: Dhpg DAYNLGTLCK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

tcp11: Hpg - Hpg - Tyr

Search NORINE for peptide: strict or relaxed

AMP-binding (459..852): Hpg

nrpys: Hpg

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: Polar, uncharged (hydroxy-phenyl) (Dhpg, Hpg)
Single AA prediction:: hpg (Hpg)

Stachelhaus prediction details:

Stachelhaus sequence:: DIFHLGLLCK
Nearest Stachelhaus code(s):: Hpg DIFHLGLLCK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1956..2348): Hpg

nrpys: Hpg

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: Polar, uncharged (hydroxy-phenyl) (Dhpg, Hpg)
Single AA prediction:: hpg (Hpg)

Stachelhaus prediction details:

Stachelhaus sequence:: DALHLGLLCK
Nearest Stachelhaus code(s):: Hpg DALHLGLLCK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (3467..3853): Tyr

nrpys: Tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Polar aromatic ring (Tyr, bOH-Tyr)
Single AA prediction:: bht (bOH-Tyr)

Stachelhaus prediction details:

Stachelhaus sequence:: DASTIAGVCK
Nearest Stachelhaus code(s):: Tyr DASTIAGVCK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

tcp12: Dhpg

Search NORINE for peptide: strict or relaxed

AMP-binding (470..870): Dhpg

nrpys: Dhpg

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: Polar, uncharged (hydroxy-phenyl) (Dhpg, Hpg)
Single AA prediction:: dhpg (Dhpg)

Stachelhaus prediction details:

Stachelhaus sequence:: DPYHGGTLCK
Nearest Stachelhaus code(s):: Dhpg DPYHGGTLCK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

tcp26: NH2

CAL_domain (44..471): NH2

minowa: NH2

Prediction, score:

NH2: 94.7
fatty_acid: 48.3
AHBA: 3.8
Acetyl-CoA: 3.4
shikimic_acid: 0.0

Link to PARAS substrate predictor